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opencc-by-4.0Jul 2019View details →

DS_LH_Tullii et al._ACS Appl. Mater. Interfaces_2019_SEM images

Scanning electron microscopy images showing P3HT pillar arrays with and without living cells on top

opencc-by-nc-sa-4.0Dec 2023View details →

SEM images of SiO2 and juniper charcoal powders (pure samples and intimate binary mixtures).

Summary: These images are those from SiO2 and juniper charcoal (JChc) powder samples observed with a SEM. The powders were obtained from commercial sources and these samples were prepared at the Bern University (Switzerland) as part of the D-A-CH/CoPhyLab project (https://www.cophylab.space/index.php?id=home) Details: ¤ SEM images from the sample of pure SiO2:     001-St5607t0_00.tif     002-St5607t0_01.tif     003-St5607t0_03.tif: ¤ SEM images from the sample of pure juniper charcoal powder:     004-St5607t6_00.tif     005-St5607t6_01.tif     006-St5607t6_04.tif     007-St5607t6_05.tif     008-St5607t6_07.tif: ¤ SEM images from the sample of the intimate mixture with 90% SiO2 - 10% JChc by mass:     009-St5606t1_00.tif     010-St5607t1_01.tif:¤ SEM images from the sample of the intimate mixture with 70% SiO2 - 30% JChc by mass:      011-St5607t2_00.tif      012-St5607t2_02.tif ¤ Zoom-in on the 70% SiO2 - 30% JChc sample at lignin fragment peppered with smaller JChc and SiO2 particles and agglomerates:      013-St5607t2_03.tif: ¤ Zoom-in on the 70% SiO2 - 30% JChc sample with apparent large fragment of lignin structure:      014-St5607t2_07.tif: ¤ SEM images from the sample of the intimate mixture with 50% SiO2 - 50% JChc by mass:      015-St5607t3_00_St5606t3_00.tif      016-St5607t3_03.tif ¤ SEM images from the sample of the intimate mixture with 30% SiO2 - 70% JChc by mass:      017-St5607t4_00.tif      018-St5607t4_01.tif,  ¤ SEM images from the sample of the intimate mixture with 10% SiO2 - 90% JChc by mass:      019-St5607t5_00.tif Addendum: These SEM images are associated with the spectroscopic and photometric data available at the following addresses:      https://doi.org/10.26302/SSHADE/EXPERIMENT_CF_20200723_000      https://doi.org/10.26302/SSHADE/EXPERIMENT_CF_20200813_000

opencc-by-4.0Aug 2021View details →

RAW SEM images mosaics dataset for the HRDIC strain localization study in shot peened Ni superalloy

We used a FEI Magellan HR 400L FE-SEM with a theoretical resolution of ≤ 0.9 nm at <1kV and ≤ 0.8 nm at ≥ 5kV to take backscattered electron images of the fine, homogeneous distributed gold speckle pattern obtained by remodelling of a thin gold layer previously deposited on the polished sample surface. The images were obtained at a working distance of 3.5 mm, 5 kV and 0.8 nA beam current. Mosaics of 30x15 images were used to cover 950x420 µm2. Each image contains 2048 x 1768 pixels and has a horizontal field of view of 43 µm. The images were overlapped by 20% to enable easy stitching prior to the digital image correlation. We obtained 7 mosaics, one before tensile testing and 6 after each deformation step. This set of images at different strain steps is coupled with the EBSD data set in https://doi.org/10.5281/zenodo.4730184, the HRDIC strain maps in http://doi.org/10.5281/zenodo.4728016 and data visualisation scripts in http://doi.org/10.5281/zenodo.4727939 0_def corresponds to the undeformed sample, while 1_def to 6_def were obtained after each deformation step.

opencc-by-4.0Apr 2023View details →

SEM image of SiO2 coated PyC tribocolloid

This SEM image shows SiO2 spheres with 4.5 micron diameter coated by Pyrolytic carbon (PyC) at 950 C for 20 min

opencc-by-4.0Jul 2023View details →

Large SEM-BSE images of hydrated belite of ages from 14 days up to 1 year

This dataset contains 8-Bit SEM-BSE images of commercially available dicalcium silicate (C2S; belite; Vustah, Czech Republic). The belite was mixed with a water/binder ratio of 0.5. The paste was the cast in small sealed containers, which were submersed with water. The specimens were stored at 22 ± 2°C. After the desired hydration times (14, 28, 84, 365 days) the hydration was stopped by immersing the prisms in isopropanol and drying them at 60°C for 12 hours. The dried prisms were then embedded in low viscosity epoxy resin and mechanically polished using diamond paste with a grain size down to 0.25 µm. Finally, the specimens were coatet with a thin layer of carbon to avoid charging. The images were acquired at 12 kV using a CBS (concentric backscatter) detector within a Thermofischer Helios G4 UX. Table 1: Basic information like resolution, size and phase composition of the images. <table> <tbody> <tr> <td>file</td> <td>age</td> <td>size</td> <td>size</td> <td>area</td> <td>pores</td> <td>hydrates</td> <td>clinker</td> </tr> <tr> <td> </td> <td>in days</td> <td>in px</td> <td>in µm</td> <td>in mm²</td> <td>area-%</td> <td>area-%</td> <td>area-%</td> </tr> <tr> <td>C2S 14d.tif</td> <td>14</td> <td>36864 x 36864</td> <td>1554.0 x 1554.0</td> <td>2.41</td> <td>31.4</td> <td>43.5</td> <td>25.2</td> </tr> <tr> <td>C2S 28d.tif</td> <td>28</td> <td>36864 x 36864</td> <td>1554.0 x 1554.0</td> <td>2.41</td> <td>15.9</td> <td>62.6</td> <td>21.5</td> </tr> <tr> <td>C2S 84d.tif</td> <td>84</td> <td>36864 x 36864</td> <td>1554.0 x 1554.0</td> <td>2.41</td> <td>19.3</td> <td>65.6</td> <td>15.2</td> </tr> <tr> <td>C2S 365d.tif</td> <td>365</td> <td>36864 x 36864</td> <td>1554.0 x 1554.0</td> <td>2.41</td> <td>23.8</td> <td>64.4</td> <td>12.0</td> </tr> </tbody> </table> The proportions of pores, hydrates and unhydrated clinker shown in table 1 are the result of manual thresholding of denoised versions of these images and may therefore differ to own measurements. The scaling is backed into the file and can be read using ImageJ/Fiji. The unstitched files are provded as 7z archives. The sub-images were arranged in a 10 x 10 grid. The pixel scaling of these files is the same as in the larger files. Internal note These files are included in the following MAPS datasets: <ul> <li>2023_05_24 C32-C2S 14-84 d</li> <li>2023_06_08 C2S-C3S 28d-1year</li> </ul> Funding The research was supported by the Deutsche Forschungsgemeinschaft (DFG), grant number <a href="https://gepris.dfg.de/gepris/projekt/344069666">344069666</a>.

opencc-by-4.0Dec 2020View details →

SEM images of PAAO samples (AJ series 1-to-5)

Scanning electron microscopy (SEM) images of porous anodized aluminum oxide (PAAO) samples. The samples were made by anodization of aluminum monocrystal in 0.3 mol/L oxalic acid at 40 V for: 3 min 16 s (AJ-1), 3 min 37 s (AJ-2), 4 min 8 s (AJ-3), 4 min 30 s (AJ-4), 4 min 57 s (AJ-5). The imaging was performed on the surface (top) of the sample, darker parts represent pores. SEM: FEI Quanta 200 FEG (FEI). Vacuum mode: high vacuum (< 6·10-4 Pa). Accelerating voltage: 20 kV. Magnification: 50 000, 100 000, 200 000.

Text-fig. 3. Upper Cretaceous to Oligocene/Miocene Sciadopityspollenites taxa, all scale bars in LM and SEM overview images 10 µm, scale bars in SEM detailed images 2 µm. a–f: cf. Sciadopityspollenites serratus from Vilui basin (Siberia), a – LM image, equatorial view, b – SEM equatorial overview with leptoma, c – detail SEM of leptoma? and echinate verrucae, d – LM image equatorial view, e – SEM of distal polar view, f – SEM detailed view of verrucate, echinate perforate sexine sculpturing; g–i: Sciadopityspollenites serratus from Bayerhof Maar (Germany), g – LM image of proximal polar side, h – SEM overview of distal polar side, i – SEM detail of verrucate, echinate, perforate sculpturing. in The Occurrence Of Pollen Of Sciadopityaceae Luerss. Through Time

Text-fig. 3. Upper Cretaceous to Oligocene/Miocene Sciadopityspollenites taxa, all scale bars in LM and SEM overview images 10 µm, scale bars in SEM detailed images 2 µm. a–f: cf. Sciadopityspollenites serratus from Vilui basin (Siberia), a – LM image, equatorial view, b – SEM equatorial overview with leptoma, c – detail SEM of leptoma? and echinate verrucae, d – LM image equatorial view, e – SEM of distal polar view, f – SEM detailed view of verrucate, echinate perforate sexine sculpturing; g–i: Sciadopityspollenites serratus from Bayerhof Maar (Germany), g – LM image of proximal polar side, h – SEM overview of distal polar side, i – SEM detail of verrucate, echinate, perforate sculpturing.

Text-fig. 2. Aptian to Albian Cerebropollenites taxa, all scale bars in LM and SEM overview images 10 µm, scale bars in SEM detailed images 2 µm. a–c: Cerebropollenites thiergartii from St. Pölten (Austria), a – LM image, equatorial view, b – SEM equatorial overview with visible, less sculptured leptoma, c – detail of echinate verrucae; d–f: Cerebropollenites thiergartii from Khovil basin (Mongolia), d – LM image polar view with well visible thin-walled leptoma, e – SEM of proximal polar view with faintly sculptures leptoma, f – SEM detailed view of transition from leptoma to normal sexine sculpturing; g–i: Cerebropollenites macroverrucosus from Khovil basin (Mongolia), g – LM image of oblique equatorial view, h – SEM of oblique equatorial view with concave leptoma, i – SEM detail of the rugulate to verrucate sexine and smaller sculpturing in leptoma area. in The Occurrence Of Pollen Of Sciadopityaceae Luerss. Through Time

Text-fig. 2. Aptian to Albian Cerebropollenites taxa, all scale bars in LM and SEM overview images 10 µm, scale bars in SEM detailed images 2 µm. a–c: Cerebropollenites thiergartii from St. Pölten (Austria), a – LM image, equatorial view, b – SEM equatorial overview with visible, less sculptured leptoma, c – detail of echinate verrucae; d–f: Cerebropollenites thiergartii from Khovil basin (Mongolia), d – LM image polar view with well visible thin-walled leptoma, e – SEM of proximal polar view with faintly sculptures leptoma, f – SEM detailed view of transition from leptoma to normal sexine sculpturing; g–i: Cerebropollenites macroverrucosus from Khovil basin (Mongolia), g – LM image of oblique equatorial view, h – SEM of oblique equatorial view with concave leptoma, i – SEM detail of the rugulate to verrucate sexine and smaller sculpturing in leptoma area.

Text-fig. 1. Extant Sciadopitys verticillata pollen. a–c: LM images (scale bars 10 µm), a – polar view, b – equatorial view, c – equatorial view with well visible thinning of proximal leptoma; d–e: SEM overview images (scale bar 10 µm), d – distal polar view, e – oblique equatorial view; f – equatorial view with leptoma at top; g–i: SEM detailed images (scale bars 2 µm), g – detail of verrucate, echinate, perforate sexine of distal pol, h – wall break displaying thin nexine and verrucate, echinate sexine, i – ripped open leptoma displaying transition from verrucate sculpturing to nearly psilate state. in The Occurrence Of Pollen Of Sciadopityaceae Luerss. Through Time

Text-fig. 1. Extant Sciadopitys verticillata pollen. a–c: LM images (scale bars 10 µm), a – polar view, b – equatorial view, c – equatorial view with well visible thinning of proximal leptoma; d–e: SEM overview images (scale bar 10 µm), d – distal polar view, e – oblique equatorial view; f – equatorial view with leptoma at top; g–i: SEM detailed images (scale bars 2 µm), g – detail of verrucate, echinate, perforate sexine of distal pol, h – wall break displaying thin nexine and verrucate, echinate sexine, i – ripped open leptoma displaying transition from verrucate sculpturing to nearly psilate state.

Text-fig. 8. SEM (a, b) and SRXTM (c, d) images of fruit associated with Miranthus elegans and Miranthus kvacekii; Mira locality, Portugal. a: Apical view of capsular fruit with five, partly open valves revealing the enclosed reticulate seeds (arrows). b: Detail of fruit wall showing an enclosed seed (arrow). c: Transverse section (orthoslice xy1200) of fruit showing central column (cc) of placenta and numerous angular and bitegmic seeds; note that the outer integument (black arrow) is thicker than inner integument (white arrow). d: Longitudinal section (orthoslice yz1239) of fruit showing perigynous attachment of calyx, central column (cc) of the placenta and sections through seeds. Specimen, Mira 99-S156331 (a–d). Scale bars = 600 µm (a, c, d), 200 µm (b). in Early Flowers Of Primuloid Ericales From The Late Cretaceous Of Portugal And Their Ecological And Phytogeographic Implications

Text-fig. 8. SEM (a, b) and SRXTM (c, d) images of fruit associated with Miranthus elegans and Miranthus kvacekii; Mira locality, Portugal. a: Apical view of capsular fruit with five, partly open valves revealing the enclosed reticulate seeds (arrows). b: Detail of fruit wall showing an enclosed seed (arrow). c: Transverse section (orthoslice xy1200) of fruit showing central column (cc) of placenta and numerous angular and bitegmic seeds; note that the outer integument (black arrow) is thicker than inner integument (white arrow). d: Longitudinal section (orthoslice yz1239) of fruit showing perigynous attachment of calyx, central column (cc) of the placenta and sections through seeds. Specimen, Mira 99-S156331 (a–d). Scale bars = 600 µm (a, c, d), 200 µm (b).

Text-fig. 7. SEM (a) and SRXTM (b–e) images of Miranthus kvacekii sp. nov.; Mira locality, Portugal. a: Lateral view of flower bud showing corolla lobes extending beyond calyx; note surface of pedicel, calyx and corolla with small equiaxial epidermal cells and indumentum of densely spaced, short stiff trichomes. b, c: Longitudinal sections through floral bud in two directions perpendicular to each other (a, orthoslice yz1024; b, orthoslice xz0950) showing corolla (co), calyx (ca), stamens (st) and semi-inferior ovary with thin ovary wall (ow) and central mushroom-shaped globose placenta (pl) bearing numerous ovules (ov). d, e: Transverse sections through floral bud above placenta (d, orthoslice xy0915; e, orthoslice xy1095) showing calyx (ca), corolla (co), ovary wall (ow) and ovules (ov); yellow outlines indicate the positions of anthers (d) and filaments (e); orange outlines indicate the position of three of the possible staminodes. Specimen, Mira 100-S170157 (a–e, holotype). Scale bars = 600 µm (a–c), 300 µm (d, e). in Early Flowers Of Primuloid Ericales From The Late Cretaceous Of Portugal And Their Ecological And Phytogeographic Implications

Text-fig. 7. SEM (a) and SRXTM (b–e) images of Miranthus kvacekii sp. nov.; Mira locality, Portugal. a: Lateral view of flower bud showing corolla lobes extending beyond calyx; note surface of pedicel, calyx and corolla with small equiaxial epidermal cells and indumentum of densely spaced, short stiff trichomes. b, c: Longitudinal sections through floral bud in two directions perpendicular to each other (a, orthoslice yz1024; b, orthoslice xz0950) showing corolla (co), calyx (ca), stamens (st) and semi-inferior ovary with thin ovary wall (ow) and central mushroom-shaped globose placenta (pl) bearing numerous ovules (ov). d, e: Transverse sections through floral bud above placenta (d, orthoslice xy0915; e, orthoslice xy1095) showing calyx (ca), corolla (co), ovary wall (ow) and ovules (ov); yellow outlines indicate the positions of anthers (d) and filaments (e); orange outlines indicate the position of three of the possible staminodes. Specimen, Mira 100-S170157 (a–e, holotype). Scale bars = 600 µm (a–c), 300 µm (d, e).

Text-fig. 2. SEM images of Miranthus elegans gen. et sp. nov.; Mira locality, Portugal. a, b: Flowers in oblique lateral view showing remains of calyx and slightly semi-inferior ovary with elongated apical style (a); note larger openings in the floral tissue (asterisk) interpreted as schizogenous secretory cavities and the stomata-like secretory structures on the upper portion of the ovary (arrows) that are interpreted as nectariferous (b). c: Detail of ovary surface showing secretory stomata-like structures (arrows). d: Flower in lateral view showing fragmentary calyx and broken slightly semi-inferior ovary with secretory stomata-like structures; note the point of attachment of the central placenta (pl). e: Cluster of seeds removed from the ovary in (d) showing reticulate surface. f: Outer (abaxial) surface of calyx lobe showing the slightly pointed papillae and scattered, fine trichomes (arrows). g: Triaperturate pollen grains from the ovary surface. Specimens, Mira 100-S153146 (a, b), Mira 100-S170155 (c), Mira 100-S101266 (d, e), Mira 105-S100732 (f), Mira 100-S170125 (g). Scale bars = 600 µm (a, b, d), 300 µm (f), 100 µm (c, e), 10 µm (g). in Early Flowers Of Primuloid Ericales From The Late Cretaceous Of Portugal And Their Ecological And Phytogeographic Implications

Text-fig. 2. SEM images of Miranthus elegans gen. et sp. nov.; Mira locality, Portugal. a, b: Flowers in oblique lateral view showing remains of calyx and slightly semi-inferior ovary with elongated apical style (a); note larger openings in the floral tissue (asterisk) interpreted as schizogenous secretory cavities and the stomata-like secretory structures on the upper portion of the ovary (arrows) that are interpreted as nectariferous (b). c: Detail of ovary surface showing secretory stomata-like structures (arrows). d: Flower in lateral view showing fragmentary calyx and broken slightly semi-inferior ovary with secretory stomata-like structures; note the point of attachment of the central placenta (pl). e: Cluster of seeds removed from the ovary in (d) showing reticulate surface. f: Outer (abaxial) surface of calyx lobe showing the slightly pointed papillae and scattered, fine trichomes (arrows). g: Triaperturate pollen grains from the ovary surface. Specimens, Mira 100-S153146 (a, b), Mira 100-S170155 (c), Mira 100-S101266 (d, e), Mira 105-S100732 (f), Mira 100-S170125 (g). Scale bars = 600 µm (a, b, d), 300 µm (f), 100 µm (c, e), 10 µm (g).

Text-fig. 1. SEM images of flowers of Miranthus elegans gen. et sp. nov.; Mira locality, Portugal. a, b: Flowers in lateral view showing elongated pedicel, narrowly triangular sepals and elongated protruding style (a); note the large openings in the floral tissue and pedicel (asterisks) interpreted as schizogenous secretory cavities. c: Flower in lateral view with portion of the calyx missing exposing the ovary wall and slightly raised nectariferous ring with probable stomata-like secretory structures (arrow). d: Flower in lateral view showing long pedicel and three of the five tepals; note the elongated narrowly triangular form of the sepals. e: Flower in oblique lateral view with portion of the calyx missing exposing the ovary and elongated style. f, g: Flowers in apical view showing the bases of five sepals (f) and apex of the five-parted ovary; note larger openings in the floral tissue (asterisk) interpreted as schizogenous secretory cavities. Specimens, Mira 100-S170155 (a, holotype), Mira 100-S153145 (b, c, g), Mira 100- S101267 (d), Mira 105-S100732 (e), Mira 100-S101268 (f). Scale bars = 600 µm (a–g). in Early Flowers Of Primuloid Ericales From The Late Cretaceous Of Portugal And Their Ecological And Phytogeographic Implications

Text-fig. 1. SEM images of flowers of Miranthus elegans gen. et sp. nov.; Mira locality, Portugal. a, b: Flowers in lateral view showing elongated pedicel, narrowly triangular sepals and elongated protruding style (a); note the large openings in the floral tissue and pedicel (asterisks) interpreted as schizogenous secretory cavities. c: Flower in lateral view with portion of the calyx missing exposing the ovary wall and slightly raised nectariferous ring with probable stomata-like secretory structures (arrow). d: Flower in lateral view showing long pedicel and three of the five tepals; note the elongated narrowly triangular form of the sepals. e: Flower in oblique lateral view with portion of the calyx missing exposing the ovary and elongated style. f, g: Flowers in apical view showing the bases of five sepals (f) and apex of the five-parted ovary; note larger openings in the floral tissue (asterisk) interpreted as schizogenous secretory cavities. Specimens, Mira 100-S170155 (a, holotype), Mira 100-S153145 (b, c, g), Mira 100- S101267 (d), Mira 105-S100732 (e), Mira 100-S101268 (f). Scale bars = 600 µm (a–g).

opencc-by-4.0Aug 2022View details →

Text-fig. 2. Charred fern from diatomite of Saint-Bauzile. a: Overview of diatomite slab with large fragment of charred fern; SM.B 22258; scale bar = 1 cm. b: SEM overview image of charred fern pinnule. c: Enlargement of (b) showing details of undulating anticlinal walls of the epidermis. in Evidence For Wildfires During Deposition Of The Late Miocene Diatomites Of The Konservat-Lagerstätte Lake Saint-Bauzile (Ardèche, France) - Preliminary Results

Text-fig. 2. Charred fern from diatomite of Saint-Bauzile. a: Overview of diatomite slab with large fragment of charred fern; SM.B 22258; scale bar = 1 cm. b: SEM overview image of charred fern pinnule. c: Enlargement of (b) showing details of undulating anticlinal walls of the epidermis.

opencc-by-4.0Aug 2022View details →

Text-fig. 1. a: Map of France showing geographic position of Saint-Bauzile (source: http://d-maps.com/m/europa/france/france/ france09.gif). b: Overview of the active diatomite quarry at the Montagne d'Andance, photograph taken in 2017. c: SEM image of a frustule of pennate diatom (cf. Navicula sp.) from Saint-Bauzile. d) SEM image of frustules forming a colony of centric diatoms (cf. Diatoma sp.) from Saint-Bauzile. in Evidence For Wildfires During Deposition Of The Late Miocene Diatomites Of The Konservat-Lagerstätte Lake Saint-Bauzile (Ardèche, France) - Preliminary Results

Text-fig. 1. a: Map of France showing geographic position of Saint-Bauzile (source: http://d-maps.com/m/europa/france/france/ france09.gif). b: Overview of the active diatomite quarry at the Montagne d'Andance, photograph taken in 2017. c: SEM image of a frustule of pennate diatom (cf. Navicula sp.) from Saint-Bauzile. d) SEM image of frustules forming a colony of centric diatoms (cf. Diatoma sp.) from Saint-Bauzile.

opencc-by-4.0Oct 2022View details →

Fig. 3. SEM images. A–D in New Coccinellidae (Coleoptera, Coccinelloidea) from Napo Province in Ecuador

Fig. 3. SEM images. A–D. Eupalea borowieci sp. nov., paratype (MIZ). E. Hyperaspis rutai sp. nov., paratype (MIZ). F. Hinda ecuadorica Gordon & Canepari, 2013, ♂, (MIZ). A. Head and pronotum. B. Head, ventral. C. Prosternum and mesoventrite. D. Habitus, ventral view. E. Head, dorsal view. F. Distal part of abdomen, ventral view.

opencc-by-4.0Jan 2022View details →

РИС. 8. Примеры проблем с иЗображением при работе на СЭМ. А, В. Засветка раЗличных частей раковин глохидиев (А. Anodonta anatina (=Colletopterum), оЗ. Красное, ХакасиЯ. В. Inversiunio reinianus, оЗ. Бива, о-в Хонсю, ЯпониЯ). C. РаЗнаЯ скорость сканированиЯ (слева – очень быстраЯ, справа – медленнаЯ) наружной поверхности глохидиЯ (Anodonta cygnea, р. Ялма, МосковскаЯ обл.). D. Артефакты в виде гориЗонтальных полос вследствие накоплениЯ отрицательного ЗарЯда при недостаточном напылении внутренней поверхности глохидиЯ (Nodularia douglasiae, ПетровскаЯ протока, бассейн р. Амур, Хабаровский кр.). МасШтаб 50 мкм (А, В), 2 мкм (С), 5 мкм (D). Микроскопы Zeiss EVO 40 (А, С, D), Zeiss MERLIN (В), напыление углеродом (А, С), хромом (В, D). FIG. 8. Illustration of different problems with SEM images. A, B. Overall illumination of some glochidia shells parts (A. Anodonta anatina (= Colletopterum), Krasnoe Lake, Khakassia. B. Inversiunio reinianus, Biwa Lake, Honshu Island, Japan). C. Different scanning speed (faster on the left and slower on the right) of the exterior glochidia valve (Anodonta cygnea, Yalma River, Moscow Oblast). D. Artifacts as horizontal stripes because of additional accumulation of a negative charge due to insufficient coating of the interior glochidia valve (Nodularia douglasiae, Petrovskaya channel, Amur River basin, Khabarovsk Krai). Scale bars 50 μm (A, B), 2 μm (C), 5 μm (D). Zeiss EVO 40 (A, C, D) and Zeiss MERLIN (B) microscopes, sputter coating with carbon (A, C) and chromium (B, D). in Методика подготовки раковин глохидиев (Bivalvia, Unionidae) длЯ работы на сканируюЩем Электронном микроскопе

РИС. 8. Примеры проблем с иЗображением при работе на СЭМ. А, В. Засветка раЗличных частей раковин глохидиев (А. Anodonta anatina (=Colletopterum), оЗ. Красное, ХакасиЯ. В. Inversiunio reinianus, оЗ. Бива, о-в Хонсю, ЯпониЯ). C. РаЗнаЯ скорость сканированиЯ (слева – очень быстраЯ, справа – медленнаЯ) наружной поверхности глохидиЯ (Anodonta cygnea, р. Ялма, МосковскаЯ обл.). D. Артефакты в виде гориЗонтальных полос вследствие накоплениЯ отрицательного ЗарЯда при недостаточном напылении внутренней поверхности глохидиЯ (Nodularia douglasiae, ПетровскаЯ протока, бассейн р. Амур, Хабаровский кр.). МасШтаб 50 мкм (А, В), 2 мкм (С), 5 мкм (D). Микроскопы Zeiss EVO 40 (А, С, D), Zeiss MERLIN (В), напыление углеродом (А, С), хромом (В, D). FIG. 8. Illustration of different problems with SEM images. A, B. Overall illumination of some glochidia shells parts (A. Anodonta anatina (= Colletopterum), Krasnoe Lake, Khakassia. B. Inversiunio reinianus, Biwa Lake, Honshu Island, Japan). C. Different scanning speed (faster on the left and slower on the right) of the exterior glochidia valve (Anodonta cygnea, Yalma River, Moscow Oblast). D. Artifacts as horizontal stripes because of additional accumulation of a negative charge due to insufficient coating of the interior glochidia valve (Nodularia douglasiae, Petrovskaya channel, Amur River basin, Khabarovsk Krai). Scale bars 50 μm (A, B), 2 μm (C), 5 μm (D). Zeiss EVO 40 (A, C, D) and Zeiss MERLIN (B) microscopes, sputter coating with carbon (A, C) and chromium (B, D).

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SI Figure 4: SEM images of either unwashed (left) or washed (right) E. antarcticus nematodes. A. Unwashed head region with arrows pointing to attached material and possible fungal hyphae. B. Washed head region with arrows pointing to the remaining attached material. C. Unwashed annules with arrows pointing to commonly attached foreign material. D. Washed annules with arrows pointing to remaining attached material. E. Unwashed somatic pore with arrows pointing to the common organic material. F. Washed vulva with an arrow pointing to remaining attached organic material. G. Unwashed cuticle with arrows showing a possible biofilm. H. Washed cuticle showing single attached cells indicated with arrows. I. Unwashed cuticle showing an off-axis line of attached material. J. Washed cuticle showing a similar off-axis line of material (as indicated with arrow) but reduced in quantity compared to the unwashed. in External and internal microbiomes of Antarctic nematodes are distinct, but more similar to each other than the surrounding environment

SI Figure 4: SEM images of either unwashed (left) or washed (right) E. antarcticus nematodes. A. Unwashed head region with arrows pointing to attached material and possible fungal hyphae. B. Washed head region with arrows pointing to the remaining attached material. C. Unwashed annules with arrows pointing to commonly attached foreign material. D. Washed annules with arrows pointing to remaining attached material. E. Unwashed somatic pore with arrows pointing to the common organic material. F. Washed vulva with an arrow pointing to remaining attached organic material. G. Unwashed cuticle with arrows showing a possible biofilm. H. Washed cuticle showing single attached cells indicated with arrows. I. Unwashed cuticle showing an off-axis line of attached material. J. Washed cuticle showing a similar off-axis line of material (as indicated with arrow) but reduced in quantity compared to the unwashed.