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Dataset results
17 results for “Scophthalmus maximus”
Proteomics reveals multiple effects of titanium dioxide and silver nanoparticles in the metabolism of turbot, Scophthalmus maximus
<p>Titanium dioxide (TiO2) and silver (Ag) NPs are among the most used engineered inorganic nanoparticles (NPs);<br> however, their potential effects to marine demersal fish species, are not fully understood. Therefore, this study<br> aimed to assess the proteomic alterations induced by sub-lethal concentrations citrate-coated 25 nm (“P25”) TiO2<br> or polyvinylpyrrolidone (PVP) coated 15 nm Ag NPs to turbot, Scophthalmus maximus. Juvenile fish were exposed<br> to the NPs through daily feeding for 14 days. The tested concentrations were 0, 0.75 or 1.5 mg of each NPs per kg of fish per day. The determination of NPs, Titanium and Ag levels (sp-ICP-MS/ICP-MS) and histological alterations<br> (Transmission Electron Microscopy) supported proteomic analysis performed in the liver and kidney.<br> Proteomic sample preparation procedure (SP3) was followed by LC-MS/MS. Label-free MS quantification<br> methods were employed to assess differences in protein expression. Functional analysis was performed using<br> STRING web-tool. KEGG Gene Ontology suggested terms were discussed and potential biomarkers of exposure<br> were proposed. Overall, data shows that liver accumulated more elements than kidney, presented more histological<br> alterations (lipid droplets counts and size) and proteomic alterations. The Differentially Expressed Proteins<br> (DEPs) were higher in Ag NPs trial. The functional analysis revealed that both NPs caused enrichment of<br> proteins related to generic processes (metabolic pathways). Ag NPs also affected protein synthesis and nucleic<br> acid transcription, among other processes. Proteins related to thyroid hormone transport (Serpina7) and calcium<br> ion binding (FAT2) were suggested as biomarkers of TiO2 NPs in liver. For Ag NPs, in kidney (and at a lower<br> degree in liver) proteins related with metabolic activity, metabolism of exogenous substances and oxidative stress<br> (e.g.: NADH dehydrogenase and Cytochrome P450) were suggested as potential biomarkers. Data suggests<br> adverse effects in turbot after medium/long-term exposures and the need for additional studies to validate<br> specific biological applications of these NPs.</p>
Data from: Exploitation of a turbot (Scophthalmus maximus L.) immune-related expressed sequence tag (EST) database for microsatellite screening and validation
In this study, we identified and characterized 160 microsatellite loci from an expressed sequence tag (EST) database generated from immune-related organs of turbot (Scophthalmus maximus). A final set of 83 new polymorphic microsatellites were validated after the analysis of 40 individuals from Atlantic origin including both wild and farmed individuals. The allele number and the expected heterozygosity ranged from 2 to 18 and from 0.021 to 0.951, respectively. Evidences of null alleles at moderate-high frequencies were detected at six loci using population data. None of the analyzed loci showed deviations from Mendelian segregation after analysis of five full-sib families including ~92 individuals/family. The markers are used to consolidate the turbot genetic map and, since they are mostly EST-derived, they will be very useful for comparative genomic studies within flatfishes and with model fish species. Using an in silico approach, we detected significant homologies of microsatellite sequences with the EST databases of the flatfish species with highest genomic resources (Senegalese sole, Atlantic halibut, bastard halibut) at 31% of these turbot markers. The conservation of these microsatellites within Pleuronectiformes will pave the way for anchoring genetic maps of different species and identifying genomic regions related to productive traits.
Data from: Regional environmental pressure influences population differentiation in turbot (Scophthalmus maximus)
Unravelling the factors shaping the genetic structure of mobile marine species is challenging due to the high potential for gene flow. However, genetic inference can be greatly enhanced by increasing the genomic, geographic or environmental resolution of population genetic studies. Here we investigated the population structure of turbot (Scophthalmus maximus) by screening 17 random and gene-linked markers in 999 individuals at 290 geographical locations throughout the Northeast Atlantic Ocean. A seascape genetics approach with the inclusion of high resolution oceanographic data was used to quantify the association of genetic variation with spatial, temporal and environmental parameters. Neutral loci identified three subgroups: an Atlantic group, a Baltic Sea group and one on the Irish Shelf. The inclusion of loci putatively under selection suggested an additional break in the North Sea, subdividing southern from northern Atlantic individuals. Environmental and spatial seascape variables correlated marginally with neutral genetic variation, but explained significant proportions (respectively 8.7 % and 10.3 %) of adaptive genetic variation. Environmental variables associated with outlier allele frequencies included salinity, temperature, bottom shear stress, dissolved oxygen concentration and depth of the pycnocline. Furthermore, levels of explained adaptive genetic variation differed markedly among basins (3% vs 12% in the North and Baltic Sea, respectively). We suggest that stable environmental selection pressure contributes to relatively strong local adaptation in the Baltic Sea. Our seascape genetic approach using a large number of sampling locations and associated oceanographic data proved useful for the identification of population units as the basis of management decisions.
Data from: Parallel evolution and adaptation to environmental factors in a marine flatfish: implications for fisheries and aquaculture management of the turbot (Scophthalmus maximus)
Unraveling adaptive genetic variation represents, in addition to the estimate of population demographic parameters, a cornerstone for the management of aquatic natural living resources, which in turn, represent the raw material for breeding programs. The turbot (Scophthalmus maximus) is a marine flatfish of high commercial value living on the European continental shelf. While wild populations are declining, aquaculture is flourishing in Southern Europe. We evaluated the genetic structure of turbot throughout its natural distribution range (672 individuals; 20 populations) by analyzing allele frequency data from 755 Single Nucleotide Polymorphism discovered and genotyped by Double Digest RAD Sequencing. The species was structured into four main regions: Baltic Sea, Atlantic Ocean, Adriatic Sea and Black Sea, with subtle differentiation apparent at the distribution margins of the Atlantic region. Genetic diversity and effective population size estimates were highest in the Atlantic populations, the area of greatest occurrence, while turbot from other regions showed lower levels, reflecting geographical isolation and reduced abundance. Divergent selection was detected within and between the Atlantic Ocean and Baltic Sea regions, and also when comparing these two regions with the Black Sea. Evidence of parallel evolution was detected between the two low salinity regions, the Baltic and Black seas. Correlation between genetic and environmental variation indicated that temperature and salinity were probably the main environmental drivers of selection. Mining around the four genomic regions consistently inferred to be under selection identified candidate genes related to osmoregulation, growth and resistance to diseases. The new insights are useful for the management of turbot fisheries and aquaculture by providing the baseline for evaluating the consequences of turbot releases from restocking and farming.
Data from: Regional environmental pressure influences population differentiation in turbot (Scophthalmus maximus)
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PAML data from: Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus)
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Data from: Parallel evolution and adaptation to environmental factors in a marine flatfish: implications for fisheries and aquaculture management of the turbot (Scophthalmus maximus)
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Data from: Exploitation of a turbot (Scophthalmus maximus L.) immune-related expressed sequence tag (EST) database for microsatellite screening and validation
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Modulation of the immune response of turbot (Scophthalmus maximus) after infection with viral haemorragic septicaemia virus
GEO Series GSE35804. Scophthalmus maximus. 16 samples. Type: Expression profiling by array.
Gene expression profiles of spleen, liver and head kidney in turbot (Scophthalmus maximus) along the infection process with Philasterides dicentrarchi using an immune-enriched oligo-microarray
GEO Series GSE35184. Scophthalmus maximus. 18 samples. Type: Expression profiling by array.
Use of an immune-specific microarray for identifying transcriptome profiles associated to VHSV infection or vaccination with a G gene- DNA vaccine in turbot (Scophthalmus maximus)
GEO Series GSE56487. Scophthalmus maximus. 68 samples. Type: Expression profiling by array.
RNA-seq analysis reveals significant transcriptome changes in turbot (Scophthalmus maximus) suffering severe enteromyxosis
GEO Series GSE63911. Scophthalmus maximus. 12 samples. Type: Expression profiling by high throughput sequencing.
Design and performance of a turbot (Scophthalmus maximus) oligo-microarray based on ESTs from immune tissues
GEO Series GSE17357. Scophthalmus maximus. 32 samples. Type: Expression profiling by array.
HEPATIC GENE EXPRESSION PATTERNS IN JUVENILE TURBOTS (Scophthalmus maximus) AFTER EXPOSURE TO NO2 FUEL OIL (PRAGMA, a pragmatic and integrated approach for the evaluation of environmental impact of oi
GEO Series GSE43361. Scophthalmus maximus. 11 samples. Type: Expression profiling by array.
HEPATIC GENE EXPRESSION PATTERNS IN JUVENILE TURBOTS (Scophthalmus maximus) AFTER EXPOSURE TO STYRENE (PRAGMA, a pragmatic and integrated approach for the evaluation of environmental impact of oil an
GEO Series GSE43359. Scophthalmus maximus. 25 samples. Type: Expression profiling by array.
HEPATIC GENE EXPRESSION PATTERNS IN JUVENILE TURBOTS (Scophthalmus maximus) AFTER EXPOSURE TO NO2 FUEL OIL AND TO STYRENE (PRAGMA, a pragmatic and integrated approach for the evaluation of environment
GEO Series GSE43355. Scophthalmus maximus. 15 samples. Type: Expression profiling by array.
Gene expression profile at single cell level of blood leukocytes from the turbot (Scophthalmus maximus)
GEO Series GSE224979. Scophthalmus maximus. 1 samples. Type: Expression profiling by high throughput sequencing.
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International Brain Laboratory public data
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OpenNeuro
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