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27 results for “Serratia marcescens”
Data from: Serratia marcescens Shapes Cutaneous Bacterial Communities and Influences Survival of an Amphibian Host
A changing understanding of the functional interactions between microbial communities and their associated hosts is influencing how disease is perceived and ameliorated. Of the numerous host-microbiome-disease systems of study, the emergence of chytridiomycosis in anurans (caused by Batrachochytrium dendrobatidis, hereafter Bd), has been implicated in ongoing declines and extinction events of amphibians across the planet. Interestingly, there has been differential survival among amphibians in resisting Bd infection and subsequent disease. One factor thought to contribute to this resistance is the host-associated cutaneous microbiota. This has raised the possibility of utilizing genetically modified probiotics to restructure the host-associated microbiota for desired anti-fungal outcomes. Here, we utilize a previously described strain of Serratia marcescens (Sm) for manipulation of amphibian cutaneous microbiota. Sm was genetically altered to have a dysfunctional pathway for the production of the extracellular metabolite prodigiosin. This genetically altered strain (Δpig) and the functional prodigiosin producing strain (WT-pig) were compared for their microbial community and anti-Bd effects both in vitro and in vivo. In vitro, Bd growth was significantly repressed in the presence of prodigiosin. In vivo, the inoculation of both Sm strains was shown to significantly influence amphibian microbiota diversity with the Δpig-Sm treatment showing increasing α-diversity, and the WT-pig S. marcescens having no temporal effect on diversity. Differences were also seen in host mortality with Δpig-Sm exhibiting significantly decreased survival probability as compared to a no¬-Sm control in the presence of Bd. These results are important evidence for the potential of genetic-level manipulation of bacteria in a host microbial community, which may provide a way to alter disease outcomes and address critical frontiers in disease and microbial ecology.
Phenotypic, genetic, and epigenetic data from 29 Serratia marcescens strains from an evolution experiment
<p>This dataset contains phenotypic, genetic, and epigenetic data from 29 <em>Serratia marcescens</em> strains from an evolution experiment. In a previous study (https://doi.org/10.1111/evo.12148), the bacterium <em>S. marcescens</em> was left to evolve from a common ancestor culture in replicated populations kept under different temperature regimes, and evolved clones were isolated and their phenotypes measured. Here, we randomly selected 28 evolved clones from this experiment, as well as the original reference strain, and used PacBio single molecule real-time (SMRT) sequencing to obtain genetic and epigenetic (N6-methyladenine modifications, m6A) data. The goal of our study was to obtain a detailed description of the methylation landscape of <em>S. marcescens</em> and to examine the potential contributions of genetic and epigenetic changes to phenotypic adaptation.</p>
Genome assemblies: Horizontal transfer of pOXA-48 from a hypervirulent Klebsiella pneumoniae ST23/KL57 to Serratia marcescens
<p>Hypervirulent Klebsiella pneumonia isolates express a range of virulence factors, often encoded on virulence plasmids. Occasionally these hypervirulent lineages acquire antimicrobial resistance genes rendering them multidrug-resistant. We describe the nosocomial transmission of a hypervirulent K. pneumoniae ST23/KL57 isolate carrying an NDM-1 and OXA-48 beta-lactamase among COVID-19 patients in a Danish university hospital. Furthermore, we characterize the plasmid structure and describe a within-patient horizontal transfer of a plasmid carrying a blaOXA-48 gene from K. pneumoniae ST23/KL57 to a Serratia marcescens isolate.</p>
Phenotypic, genetic, and epigenetic data from 29 Serratia marcescens strains from an evolution experiment
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Data from: Serratia marcescens Shapes Cutaneous Bacterial Communities and Influences Survival of an Amphibian Host
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Data from: Dauer life stage of Caenorhabditis elegans induces elevated levels of defense against the parasite Serratia marcescens
Host-parasite research often focuses on a single host life stage, yet different life stages may exhibit different defenses. The nematode Caenorhabditis elegans has an alternate dispersal life stage, dauer. Despite dauer's importance in nature, we know little of how it responds to parasites. Previous research indicates that non-dauer C. elegans prefer to consume the virulent bacterial parasite, Serratia marcescens, when given a choice between the parasite and benign Escherichia coli. Here, we compared the preferences of dauer individuals from six strains of C. elegans to the preferences of other life stages. We found that dauer individuals exhibited reduced preference for S. marcescens, and dauers from some strains preferred E. coli to S. marcescens. In addition to testing food preference, a mechanism of parasite avoidance, we also measured host mortality rates after direct parasite exposure to determine if life stage also altered host survival. Overall, dauer individuals exhibited reduced mortality rates. However, dauer versus non-dauer larvae mortality rates also varied significantly by host strain. Collectively, we found evidence of dauer-induced parasite avoidance and reduced mortality in the presence of a parasite, but these effects were strain-specific. These results demonstrate the importance of host life stage and genotype when assessing infection dynamics.
Application strategies by selective medium treated with entomopathogenic bacteria Serratia marcescens and Pseudomonas aeruginosa as potential biocontrol against Coptotermes curvignathus
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Data from: Dauer life stage of Caenorhabditis elegans induces elevated levels of defense against the parasite Serratia marcescens
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Data from: No measurable fitness cost to experimentally evolved host defense in the Caenorhabditis elegans-Serratia marcescens host-parasite system
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Spaceflight and simulated microgravity conditions increase virulence of Serratia marcescens in the Drosophila melanogaster infection model
GEO Series GSE138116. Drosophila melanogaster. 12 samples. Type: Expression profiling by high throughput sequencing.
Competitive behaviours in Serratia marcescens are coordinately regulated by a lifestyle switch frequently inactivated in the clinical environment
GEO Series GSE273522. Serratia marcescens. 20 samples. Type: Expression profiling by high throughput sequencing.
Transcriptomic Analysis of the Serratia marcescens Response to Batrachochytrium dendrobatidis
GEO Series GSE84057. Serratia marcescens. 6 samples. Type: Expression profiling by high throughput sequencing.
Drosophila melanogaster midgut transcriptome analysis after an oral infection by the entomopathogen bacteria Serratia marcescens
GEO Series GSE60504. Drosophila melanogaster. 6 samples. Type: Expression profiling by high throughput sequencing.
C. elegans young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours
GEO Series GSE36499. Caenorhabditis elegans. 4 samples. Type: Expression profiling by array.
The Serratia marcescens siderophore, serratiochelin, is necessary for full virulence during bloodstream infection
GEO Series GSE145587. Serratia marcescens. 4 samples. Type: Expression profiling by high throughput sequencing.
P. pacificus young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours
GEO Series GSE36521. Pristionchus pacificus. 4 samples. Type: Expression profiling by array.
Spaceflight and simulated microgravity conditions increase virulence of Serratia marcescens in the Drosophila melanogaster infection model
While it has been shown that astronauts suffer immune disorders after spaceflight, the underlying causes are still poorly understood and there are many variables to consider when investigating the immune system in a complex environment. Additionally, there is growing evidence that suggests that not only is the immune system being altered, but the pathogens that infect the host are significantly influenced by spaceflight and ground-based spaceflight conditions. In this study, we demonstrate that Serratia marcescens (strain Db11) was significantly more lethal to Drosophila melanogaster after growth on the International Space Station than ground-based controls, but that the host immune system is not significantly altered amongst known immune genes. High-throughput sequencing of wild-type (w1118) adult hosts infected with either space or ground-reared S. marcescens revealed few changes in gene expression, with 11 genes significantly differentially expressed (q-values less than 0.05) and only one gene related to the immune system. This data supports the main findings of the paper, which state that both spaceflight and low-shear modeled microgravity conditions increase the virulence of this pathogen, independent of the host immune system. This data, which shows that there are no significant immune-related changes to the host when infected with space-grown sample compared to ground-grown sample, provides further evidence that there are likely phenotypic changes to the pathogen itself that is causing increased virulence in spaceflight and in low-shear modeled microgravity. RNA was extracted in triplicate from 2 pooled adult (2-3 day old female) Drosophila melanogaster (w1118) per treatment, with 4 total treatment groups (no injection control, sham injection with PBS, ground bacteria-injected, and space bacteria-injected) with poly(A)+ RNA libraries. Samples were multiplexed and sequenced 100bp paired-end ready were sequenced on one lane of the Illumina HiSeq-4000.
An XRE-superfamily transcriptional regulator XrpA controls low pH tolerance of Serratia marcescens
GEO Series GSE137185. Serratia marcescens WW4. 2 samples. Type: Expression profiling by high throughput sequencing.
Transcriptional impact of eepR mutation in Serratia marcescens
GEO Series GSE155648. Serratia marcescens. 8 samples. Type: Expression profiling by high throughput sequencing.
Response of human corneal epithelial cells to the secretomes of wild-type Serratia marcescens and an eepR mutant strain
GEO Series GSE71320. Homo sapiens. 6 samples. Type: Expression profiling by array.
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Allen Brain Atlas
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International Brain Laboratory public data
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OpenNeuro
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