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FIGURE 3. Simplicillium coffeanum COAD 2057. A in Simplicillium coffeanum, a new endophytic species from Brazilian coffee plants, emitting antimicrobial volatiles

FIGURE 3. Simplicillium coffeanum COAD 2057. A, Host plant; B, colony; C, Colony reverse; D–F, Hypha, phialides and conidia. Scale bars = 10 μm

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FIGURE 1 in Simplicillium coffeanum, a new endophytic species from Brazilian coffee plants, emitting antimicrobial volatiles

FIGURE 1. Bayesian Inference tree showing the phylogenetic relationship between Simplicillium coffeanum and closely taxa based on partial 28S rDNA sequences. The posterior probability values are indicated at the nodes. The isolates from this study are highlighted in bold. The tree is rooted with Ceratocystis moniliformis. Asterisks indicate the type strains.

opennotspecifiedJan 2018View details →

FIGURE 2 in Simplicillium coffeanum, a new endophytic species from Brazilian coffee plants, emitting antimicrobial volatiles

FIGURE 2. Bayesian Inference tree of Simplicillium and closely Cordycipitaceae using ITS-5.8S sequences of rDNA. The posterior probability values are indicated at the nodes. The Simplicillium isolates from this study are highlighted in bold. The tree is rooted with Pochonia chlamydosporia. Asterisks indicate the type strains.

FIGURE 4 in Simplicillium pech-merlensis, a new fungal species isolated from the air of the Pech-Merle show cave

FIGURE 4. Phylogenetic placement of Simplicillium pech-merlensis within Simplicillium and Leptobacillium genera assessed through Bayesian inference. Unrooted tree obtained from a dataset including 84 taxa, leading to a concatenated alignment of 4725 bp from six nuclear regions (ITS, LSU, SSU, RPB1, RPB2 and TEF1-α). Bayesian posterior probabilities greater than or equal to 0.50 are located close to the corresponding node. The newly described species is indicated in red.

FIGURE 2 in Simplicillium pech-merlensis, a new fungal species isolated from the air of the Pech-Merle show cave

FIGURE 2. Isolation of Simplicillium pech-merlensis. The species was isolated near the "injured man" panel in the Pech-Merle show cave, via air samplings with impaction on Malt Extract Agar (MEA).

FIGURE 1 in Simplicillium pech-merlensis, a new fungal species isolated from the air of the Pech-Merle show cave

FIGURE 1. Pointed-horses panel in the Pech-Merle show cave The panel represents the paintings of dappled horses and negative hands for which the Pech-Merle cave is known. The representations were mainly drawn with black pigments composed of manganese and barium oxides as well as charcoal (Lorblanchet 2018). The drawing features the use of red pigments composed of red ochre.

FIGURE 3 in Simplicillium pech-merlensis, a new fungal species isolated from the air of the Pech-Merle show cave

FIGURE 3. Phylogenetic placement of Simplicillium pech-merlensis within Simplicillium genus and Simplicillium related genera assessed through maximum likelihood. Unrooted tree obtained from a dataset including 84 taxa, leading to a concatenated alignment of 4725 bp from seven nuclear regions (ITS, LSU, SSU, RPB1, RPB2 and TEF1-α). Bootstrap values greater than or equal to 50 % are located close to the corresponding node. The newly described species is indicated in red.

Figure 5 from: Chen W-H, Liu C, Han Y-F, Liang J-D, Tian W-Y, Liang Z-Q (2019) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83-102. https://doi.org/10.3897/mycokeys.58.37176

Figure 5 Simplicillium formicidaeA isolated substrate an infected ant (Hymenoptera) B–C culture plate, showing the front (B) and the reverse (C) of the colony, cultured on PDA medium D, E phialides solitary, conidia adhering globose slimy head and conidia F conidia. Scale bars: 10 mm (B, C), 10μm (D, E, F).

Figure 4 from: Chen W-H, Liu C, Han Y-F, Liang J-D, Tian W-Y, Liang Z-Q (2019) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83-102. https://doi.org/10.3897/mycokeys.58.37176

Figure 4 Simplicillium lepidopterorumA infected carpenterworm (Lepidoptera) B, C culture plate, showing the front (B) and the reverse (C) of the colony, cultured on PDA medium D, E, F phialides solitary and conidia in globose heads D conidia. Scale bars: 10 mm (B, C), 10μm (D, E, F, G).

Figure 3 from: Chen W-H, Liu C, Han Y-F, Liang J-D, Tian W-Y, Liang Z-Q (2019) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83-102. https://doi.org/10.3897/mycokeys.58.37176

Figure 3 Simplicillium cicadellidaeA infected leafhopper (Hemiptera) B–C culture plate, showing the front (B) and the reverse (C) of the colony, cultured on PDA medium D–F phialides solitary, conidia adhering ellipsoidal slimy head and conidia G conidia. Scale bars: 10 mm (B, C), 10μm (D, E, F, G).

Figure 1 from: Chen W-H, Liu C, Han Y-F, Liang J-D, Tian W-Y, Liang Z-Q (2019) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83-102. https://doi.org/10.3897/mycokeys.58.37176

Figure 1 Phylogenetic relationships among the genus Simplicillium and its allies in Cordycipitaceae based on multigene dataset (LSU, RPB1, RPB2 and TEF). Statistical support values (≥ 0.5/50%) are shown at the nodes for ML bootstrap support/BI posterior probabilities. The tree is rooted with Purpureocillium lilacinum (CBS 284.36 and CBS 431.87). The new species are in bold face. T in the upper right corner indicates the type strains.

Figure 2 from: Chen W-H, Liu C, Han Y-F, Liang J-D, Tian W-Y, Liang Z-Q (2019) Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China. MycoKeys 58: 83-102. https://doi.org/10.3897/mycokeys.58.37176

Figure 2 Phylogenetic relationships among the new taxa S. cicadellidae, S. formicidae, S. lepidopterorum and other Simplicillium species by ITS+LSU sequences. Statistical support values (≥ 0.5/50%) are shown at the nodes for ML bootstrap support/BI posterior probabilities. The tree is rooted with Pochonia chlamydosporia (CBS 103.65). The new species are in bold face. T in the upper right corner indicates the type strains.

Figure 1 from: Wei D-P, Wanasinghe DN, Hyde KD, Mortimer PE, Xu J, Xiao Y-P, Bhunjun CS, To-anun C (2019) The genus Simplicillium. MycoKeys 60: 69-92. https://doi.org/10.3897/mycokeys.60.38040

Figure 1 Phylogram generated from maximum likelihood analysis based on combined SSU, LSU, TEF and RPB1 sequence data. Bootstrap values for maximum likelihood (ML, left) and maximum parsimony (MP, right) equal to or greater than 50% and Bayesian posterior probabilities (BYPP, middle) equal to or greater than 0.90 are placed nearby the note. The newly generated sequences are indicated in red bold.

Figure 6 from: Wei D-P, Wanasinghe DN, Hyde KD, Mortimer PE, Xu J, Xiao Y-P, Bhunjun CS, To-anun C (2019) The genus Simplicillium. MycoKeys 60: 69-92. https://doi.org/10.3897/mycokeys.60.38040

Figure 6 Ophiocordyceps unilateralis (from HKAS 102447) a stroma emerging from host b ascomata on stroma c host (Formicidae) d, e decayed perithecia. Scale bars: 500 µm (b, c); 300 µm (d); 100µm (e).

Figure 3 from: Wei D-P, Wanasinghe DN, Hyde KD, Mortimer PE, Xu J, Xiao Y-P, Bhunjun CS, To-anun C (2019) The genus Simplicillium. MycoKeys 60: 69-92. https://doi.org/10.3897/mycokeys.60.38040

Figure 3 Simplicillium formicae (from HKAS 102459, holotype) a superficial hyphae associated with the ant host b–e flask-shaped synnemata f–k phialides bearing conidia l–p conidia. Scale bars: 1000 µm (a); 500 µm (b); 100 µm (d); 30 µm (e, f); 15 µm (j, k); 10 µm (l–p) (e stained with cotton blue solution).

Figure 4 from: Wei D-P, Wanasinghe DN, Hyde KD, Mortimer PE, Xu J, Xiao Y-P, Bhunjun CS, To-anun C (2019) The genus Simplicillium. MycoKeys 60: 69-92. https://doi.org/10.3897/mycokeys.60.38040

Figure 4 Simplicillium formicae (MFLUCC 18–1379, ex-type living culture) a upper and reverse view of cultures on PDA after 30 days e–g phialides indicated with black arrow c, d, h–j conidial mass on the tip of phialides k–m conidia. Scale bars: 10 µm (c, d, f, g); 20 µm (e); 3 µm (h–j); 1 µm (k–m) (e–j stained with cotton blue solution).

Figure 2 from: Wei D-P, Wanasinghe DN, Hyde KD, Mortimer PE, Xu J, Xiao Y-P, Bhunjun CS, To-anun C (2019) The genus Simplicillium. MycoKeys 60: 69-92. https://doi.org/10.3897/mycokeys.60.38040

Figure 2 Phylogram generated from maximum likelihood analysis based on ITS sequence data. Bootstrap values for maximum likelihood (ML, left) and maximum parsimony (MP, right) equal to or greater than 50% and Bayesian posterior probabilities (BYPP, middle) equal to or greater than 0.90 are placed nearby the branches, respectively. The newly generated sequences are indicated in red bold and the type species are highlighted in black bold.

Figure 5 from: Wei D-P, Wanasinghe DN, Hyde KD, Mortimer PE, Xu J, Xiao Y-P, Bhunjun CS, To-anun C (2019) The genus Simplicillium. MycoKeys 60: 69-92. https://doi.org/10.3897/mycokeys.60.38040

Figure 5 Simplicillium lanosoniveum(a–f from HKAS 102447, g–r from MFLUCC 18–1385) a host (Ophiocordyceps unilateralis); b, c hyphae associated with host indicated with black arrows g, h upper and reverse view of cultures on PDA after 40 days incubation i–l conidial mass on the tip of phialides m–o phialides bearing conidia p–r conidia. Scale bars: 15 µm (i–m); 10 µm (d–f, n, o); 3 µm (p–r) (i, l–n stained with cotton blue solution).