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6 results for “Spelaeonaias”
FIGURE 6. The 50 in Spelaeonaias gen. nov., a new true-branched cyanobacterium from Cave Vlychada (Diros, Peloponnese, Greece)
FIGURE 6. The 50% majority rule consensus tree as inferred from MrBayes. Posterior probalities are provided on or below the nodes. The GenBank accession numbers of sequences are given on the right of the taxa names. The scale corresponds to substitutions/site. Characters in bold indicate the taxon from Vlychada Cave.
FIGURE 5 in Spelaeonaias gen. nov., a new true-branched cyanobacterium from Cave Vlychada (Diros, Peloponnese, Greece)
FIGURE 5. Fluorescence emission spectrum of cells obtained by the Lambda scan module of CLSM. Two-dimensional spectral characteristic of fluorescence is illuminated with the blue laser (488 nm) and recorded as arbitrary units. Note the first peak for phycoerythrin (PE) (582 nm) and the second for phycobiliproteins (PC: phycocyanin and APC: allophycocyanin) (669 nm) including the small shoulder for chlorophyll a.
FIGURE 4. A–C in Spelaeonaias gen. nov., a new true-branched cyanobacterium from Cave Vlychada (Diros, Peloponnese, Greece)
FIGURE 4. A–C. Confocal (CLSM) photomicrographs showing filaments of Spelaeonaias floccida covered by sheaths. A. 41 x-y optical sections (z step= 0.13 μm) showing the Y-type of true branching; note the central spaces devoid of fluorescence in the filaments, corresponding to the area of nucleoids. B. 30 x-y optical sections (z step= 0.13 μm) showing a degraded basal cell (dc) promoting a false branching. C. Main filament with secondary branches; division of initial cells (at arrows) implying further formation of secondary branches. Color allocation: DNA nucleic acids labelled with Hoechst 33258, cyan; reflection from minerals, white; autofluorescence of extracellular polymeric substances, green; colocalized autofluorescence of cyanobacteria in the red (phycobilins) and blue channels (chlorophylls), magenta. Scale bars = 10 μm.
FIGURE 3. A–D in Spelaeonaias gen. nov., a new true-branched cyanobacterium from Cave Vlychada (Diros, Peloponnese, Greece)
FIGURE 3. A–D. TEM micrographs of Spelaeonaias floccida showing cell division and mode of branching. A. Filament with short barrel cells showing a general view of dividing cells for branch formation; the oblique division of an initial cell at arrow. B. Oblique section at a particular part of a filament showing one heterocyte (h) with a polar plug of cyanophycin (cy) connected to the adjacent cell by a neck; note the extra wall layer (wl) surrounding the heterocyte. C. Cell division at a certain region of the filament and formation of a new septum (s); D–E. After the first division of the initial cell (ic) the resulting cell (arrow) changes polarity and is further dividing for the formation of a secondary branch. Scale bars = 2 μm (B) and 5 μm (A–D).
FIGURE 1. A–J in Spelaeonaias gen. nov., a new true-branched cyanobacterium from Cave Vlychada (Diros, Peloponnese, Greece)
FIGURE 1. A–J. LM and SEM micrographs of Spelaeonaias floccida. A. Filaments of Spelaeonaias floccida showing the main filament and the secondary branches. B. Division of an initial cell, giving rise to two cells that change division polarity for the formation of a secondary branch. C. Secondary filament with a necridic cell (nc). D–G. Main and secondary filaments with the characteristic Y-type of branching; heterocytes at arrows. H. Hormogonia with an intercalary heterocyte at arrow. I. A single hormogonium with a terminal heterocyte (arrow). J. Single hormogonium as seen under SEM; the heterocyte (arrow) in a secondary filament is also obvious. Figs A–B from fresh material. Figs C–J from cultures. Scale bars = 10 μm (A–D, F, H, I) and 20 μm (Ε, G, J).
FIGURE 2. A–F in Spelaeonaias gen. nov., a new true-branched cyanobacterium from Cave Vlychada (Diros, Peloponnese, Greece)
FIGURE 2. A–F. TEM micrographs of Spelaeonaias floccida. A. Longitudinal section showing a septum (s) without intercellular connection between vegetative cells, the outer membrane (om) and the plasma membrane (pm). Note the thick and compact sheath near the cell wall, becoming diffluent externally. the thylakoids are scattered throughout the cyatoplasm forming curled bundles of 2–4 parallel arranged membranes, with phycobilisomes (pb) on their surface. B. Grazing thin section providing a top view of phycobilisomes as stellate structures, associated with thylakoidal membranes; the plasma membrane is followed by a thin layer of peptidoglycan (pl) and an outer membrane (om). C. Carboxysomes (cb) seen as polyhedral bodies. D. Peptidoglycan layer crossed by pore structures (arrow). E–F. Nucleoid regions surrounded by thylakoids and scattered through the cytoplasm; cy = cyanophycin granule. Scale bars = 500 nm (A, C–F) and 100 nm (B).
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