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123 results for “Steinernema”
FIGURE 3. A in Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy
FIGURE 3. A, SEM of first generation female of S. apuliae sp. n., head with 6 labial and 4 cephalic papillae; B, first generation female, Nomarski LM, tail with apical papillae or protuberance; C, SEM of first generation female, tail with apical papillae or protuberances; D, SEM of first generation female, lateral view of tail with conicallike tip and apical papilla or protuberance; E, second generation female, Nomarski LM, asymmetrical vulva; F, SEM of second generation female, asymmetrical vulva; G, SEM of second generation female, ventral view of tail with an apical protuberance; H, SEM of infective juvenile, lateral field with 8 identical ridges; I, infective juvenile, Nomarski LM, esophageal portion with excretory pore and hemizonid; L, infective juvenile, Nomarski LM, tail with hyaline portion.
FIGURE 2. A in Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy
FIGURE 2. A, SEM second generation male of S. apuliae sp. n, dorsal view of posterior region of male and 2 pairs of adcloacal papillae (a and b), 3 pairs of papillae near tail tip; B, second generation male, Nomarski LM, esophagus and excretory pore; C, second generation male, Nomarski LM, mail tale with spicules and gubernaculum.
FIGURE 4 in Steinernema apuliae sp. n. (Rhabditida: Steinernematidae): a new entomopathogenic nematode from southern Italy
FIGURE 4. RFLP profiles for (A) Steinernema apuliae sp. n., (B) S. arenarium and (C) S. glaseri. M molecular weight markers (band sizes shown in base pairs); Sf, the ITS region from S. feltiae (UK site 76) digested with Alu I; lanes 1 to 17 the ITS region for each isolate digested with the following restriction enzymes; 1, Alu I; 2, BstO I; 3, Dde I; 4, EcoR I; 5, Hae III; 6, Hha I; 7, Hind III; 8, Hinf I; 9, Hpa II; 10, Kpn I; 11, Pst I; 12, Pvu II; 13, Rsa I; 14, Sal I; 15, Sau 3 A I; 16, Sau 96 I; 17, Xba I.
FIGURE 5. Steinernema guangdongense n in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 5. Steinernema guangdongense n. sp. Lightmicroscope photographs. AB, epiptygma of the first generation females. CD, tails of infective juveniles showing dorsal constriction (arrows), compared to no dorsal constriction in S. longicaudum in EF. G, mature female of the first generation with prominent postanal swelling. H, second generation female with longer tail. Scales: A, B = 18 μm, C = 24 μm, D = 22 μm, E, F = 22 μm.
FIGURE 6 in Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum)
FIGURE 6. SEM photographs of Steinernema guangdongense n. sp. infective juvenile. AB, anterior region showing one lateral line, closed mouth (m), amphids (a) and cephalic (c) papillae. C, lateral field with 2 ridges (3 incisures). D, lateral field showing the change of lateral field pattern from 2 to 7 ridges. E, lateral field showing 7 ridges and the middle one (number 4) is divided into 2 making 8 ridges in lateral field. F, lateral field showing phasmid (p) and 7 ridges changing to 4 then 2. Scales: A = 6.67 μm, B = 5 μm, C = 8.60 μm, D F = 6.67 μm.
Fig. 1 in The Effect Of Temperature On The Development Of Adult Generations Of Entomopathogenic Nematode Steinernema Arenarium Isolate Ch
Fig. 1. Scatterplots of canonical scores for females of both generations. Legend: dots — 18 °С; squares — 22 °С; rhombs — 28 °С. Ranges of groups are ellipsed with coefficient 0.95.
Fig. 2 in The Effect Of Temperature On The Development Of Adult Generations Of Entomopathogenic Nematode Steinernema Arenarium Isolate Ch
Fig. 2. Scatterplots of canonical scores for males of both generations. Legend: dots — 18 °С; squares — 22 °С; rhombs — 28 °С. Ranges of groups are ellipsed with coefficient 0.95.
Figure 3 in Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae
Figure 3: Lateral left view (anterior to left) differential interference contrast (DIC) micrograph (top) and with transparent color overlay (bottom) of a 96 HET female Steinernema carpocapsae gonad posterior arm. The gonad morphology at 96 HET is similar to that seen at 72 HET. However, by 96 HET, numerous fertilized embryos are seen in the uterus (red). The spermathecal-uterine complex (green) is located at the farthest point away from the mid-body and contains a single oocyte (inset) surrounded by numerous sperm (arrowhead). The ovary (blue) is much longer than in 72 HET females, frequently extending beyond the mid-body plane. In this image, the distal tip cell of the anterior gonad arm (arrow) extends into the posterior half of the female. Scale bars, 50 µm (main figure) 10 µm (inset).
Figure 6 in Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae
Figure 6: Lateral right (A) and left (B) views of gonad (red) morphology in male Steinernema carpocapsae at 72 HET. In most males (A), the gonad migration pattern is similar to C. elegans. However, 30% of males examined have a gonad that never extends away from the ventral wall (B). Scale bar, 50 µm.
Figure 5 in Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae
Figure 5: Lateral left views (anterior to left) of female gonad (red overlay) development in Steinernema carpocapsae at various time points. The gonad of newly hatched S. carpocapsae (A) contained four nuclei with two central larger nuclei and two smaller cap nuclei. By 25 HET (B), the number of nuclei in the gonad noticeably increased and began elongating along the ventral body wall. At 33 HET (C), the gonad extended toward the dorsal surface. Between 44 HET (D) and 54 HET (E), we observed an expansion of the gonad along the dorsal surface. Note that the anterior arm typically wraps around the right side of the intestine and is out of frame at this focal plane, whereas the posterior arm wraps around the left side. Scale bar, 50 µm.
Figure 1 in Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae
Figure 1: Lateral left view (anterior to left) fluorescent micrographs of J2 (top) and J1 (bottom) Steinernema carpocapsae following 4′, 6-diamidino2-phenylindole (DAPI) staining. The ventral nerve cord (VNC) comprises a series of putative neurons extending from the retrovesicular ganglion (RVG) (arrowhead) to the preanal ganglion (PAG) (arrow) along the ventral edge of the animal. Between J1 and J2, there is a large increase in the number of VNC neuronal-like nuclei. Scale bar, 10 µm.
Figure 2 in Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae
Figure 2: Cartoons of Caenorhabditis elegans (A) and Steinernema carpocapsae (B) gonads and lateral left view (anterior to left) micrograph of posterior S. carpocapsae gonad arm (C): differential interference contrast (DIC) alone (top); DIC color overlay (bottom). Unlike C. elegans, which undergoes stereotypical development to a 'U-shaped' gonad, the most common S. carpocapsae gonad shape does not include extensive migration along the ventral wall. As shown in the stitched DIC micrograph (C) (top) and with transparent color overlay (bottom), the gonad extends from the central region of the animal near the vulva (position indicated with straight arrow) and immediately extends to the dorsal side where it extends away from the mid-body. It eventually makes a u-turn (curved arrow) and then extends along the dorsal wall back to the midbody where it then migrates back to the ventral wall. The posterior gonad arm typically wraps around the left side of the intestine, whereas the anterior arm typically wraps around the right side of the intestine. The proximal end of the gonad, including the uterus and spermathecal-uterine complex, is colored red. The distal end of the gonad following the turn includes the ovary and is colored blue. Scale bar, 50 µm.
Figure 4 in Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae
Figure 4: Cartoon depicting the variability in the gonad migration pattern among 72 HET Steinernema carpocapsae females that deviate from the most common phenotype seen in figure 2C. Numbers indicate the ratio of the particular gonad phenotype among the 28 females whose gonads were fully traced.
Fig. 1 in Effect of Colombian strains of Steinernema colombiense (Rhabditida: Steinernematidae) and Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) against Eurhizococcus colombianus (Hemiptera: Margarodidae) and Aeneolamia sp. (Hemiptera: Cercopidae)
Fig. 1. Percentage mortality (mean ± SE) of Aeneolamia sp. nymphs treated with Steinernema colombiense and Heterorhabditis bacteriophora strain Fresno at 12 d afer inoculation under greenhouse conditions. Different letters indicate significant differences (ANOVA and Tukey's HSD test at P <0.05).
Fig. 4 in Asilidae (Diptera: Brachycera) - natural hosts of Steinernema feltiae (Rhabditida: Steinernematidae) at the Zemen Gorge region
Fig. 4. Caudal view of the male S. feltiae Fig. 3. Head capsule of the host larva. Scale isolated from the host. Scale bar: 25 bar: 0.3 mm. µm.
Fig.1 in Asilidae (Diptera: Brachycera) - natural hosts of Steinernema feltiae (Rhabditida: Steinernematidae) at the Zemen Gorge region
Fig.1. Fragment of the investigated habitat Fig.2. Nematodes emerging from the asilid with Aegopodium podagraria. host larva. Scale bar: 1 mm.
Fig. 1 in Development of Steinernema feltiae (Rhabditida: Steinernematidae) in larvae of Chaetonyx robustus (Coleoptera: Orphnidae)
Fig. 1. Imago and larvae of Chaetonyx robustus, collected in soil of the investigated habitat (22.04.2011, D. Gradinarov leg.). Scale bar: 10 mm.
Fig. 3. First parasitic generation emerging Fig. 4. Juveniles migration from a in Development of Steinernema feltiae (Rhabditida: Steinernematidae) in larvae of Chaetonyx robustus (Coleoptera: Orphnidae)
Fig. 3. First parasitic generation emerging Fig. 4. Juveniles migration from a host. from host. Scale bar: 1 mm. Scale bar: 1 mm.
Figure 7 in Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
Figure 7. Scanning electron microscopy images of Steinernema beitlechemi infective juvenile and female. A–D: Infective juvenile. A: Head region with four papillae, amphid openings (a) and excretory pore (ep); B: Lateral field in mid-body (ridges numbered 1–6); C: Lateral field in tail region with anus and phasmid opening (arrow); D: Tail region with anus and phasmid openings (arrows), ventral view. E, F: First generation female. E: Vulva; F: Tail with mucron (m), ventro-lateral.
Figure 6 in Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
Figure 6. Storage of entomopathogenic nematodes in clear tissue culture flasks on the left and Tetrapak containers on the right.
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