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Supplementary data for "The subgenual organ complex in stick insects: Functional morphology and mechanical coupling of a complex mechanosensory organ"
<p>µCT-scans of the upper tibial regions of the foreleg (T1) and the midleg (T2) of <em>Ramulus artemis</em> (Westwood, 1859), <em>Carausius morosus</em> (Sinéty, 1901), and <em>Sipyloidea sipylus</em> (Westwood, 1859). For use of scans, please cite the following publication:</p> <p>Strauß, J., Moritz, L. & Rühr, P.T. (<strong>2021</strong>): The subgenual organ complex in stick insects: Functional morphology and mechanical coupling of a complex mechanosensory organ. <em>Frontiers in Ecology and Evolution (Research Topic “Evolutionary Biomechanics of Sound Production and Reception”)</em>. doi: <a href="https://doi.org/10.3389/fevo.2021.632493">10.3389/fevo.2021.632493</a>.</p> <p>All scans were performed with a commercial μCT desktop system (Skyscan 1272, Bruker microCT, Kontich, Belgium) at the Zoological Research Museum Alexander Koenig, Leibniz Institute for Animal Biodiversity, Bonn, Germany.</p> <p><strong>µCT scan settings of all samples:</strong></p> <p><em>Ramulus artemis:</em></p> <ul> <li>tube voltage = 30 kV</li> <li>ube current = 200 μA</li> <li>target = tungsten</li> <li>no filter</li> <li>total sample rotation = 360°</li> <li>angular step size = 0.2°</li> <li>exposure time = 1980 ms</li> <li>binning = 1x1</li> <li>averaging = 8</li> <li>random movement = 15 px</li> <li>voxel size = 1.8 μm</li> <li>fixation: Bouin's solution (24 hours)</li> <li>staining: 0.3% PTA (21 days)</li> <li>storage: 70% EtOH</li> <li>surrounding medium in scan: 70% EtOH</li> <li>filenames: Ramulus_artemis_T1.tif; Ramulus_artemis_T2.tif</li> </ul> <p><em>Carausius morosus:</em></p> <ul> <li>tube voltage = 29 kV</li> <li>ube current = 200 μA</li> <li>target = tungsten</li> <li>no filter</li> <li>total sample rotation = 360°</li> <li>angular step size = 0.2°</li> <li>exposure time = 1900 ms</li> <li>binning = 1x1</li> <li>averaging = 5</li> <li>random movement = 15 px</li> <li>voxel size = 1.0 μm</li> <li>fixation: Bouin's solution (24 hours)</li> <li>staining: 0.3% PTA (21 days)</li> <li>storage: 70% EtOH</li> <li>surrounding medium in scan: 70% EtOH</li> <li>filenames: Carausius_morosus_T1.tif; Carausius_morosus_T2.tif</li> </ul> <p><em>Sipyloidea sipylus:</em></p> <ul> <li>tube voltage = 29 kV</li> <li>ube current = 200 μA</li> <li>target = tungsten</li> <li>no filter</li> <li>total sample rotation = 360°</li> <li>angular step size = 0.2°</li> <li>exposure time = 1900 ms</li> <li>binning = 1x1</li> <li>averaging = 7</li> <li>random movement = 15 px</li> <li>voxel size = 1.8 μm</li> <li>fixation: Bouin's solution (24 hours)</li> <li>staining: 0.3% PTA (21 days)</li> <li>storage: 70% EtOH</li> <li>surrounding medium in scan: 70% EtOH</li> <li>filenames: Sipyloidea_sipylus_T1.tif; Sipyloidea_sipylus_T2.tif</li> </ul>
Fig. 2 in The new genus of stick insect Lobofemora from Vietnam, with the description of three new species (Phasmida: Phasmatidae: Clitumnini)
Fig. 2. Lobofemora bachmaensis sp. nov. ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, lateral view. G. Apex of abdomen, ventral view. H. Anterior part of body, lateral view. D–H = not to scale.
Fig. 10 in The new genus of stick insect Lobofemora from Vietnam, with the description of three new species (Phasmida: Phasmatidae: Clitumnini)
Fig. 10. Lobofemora gen. nov. spp., photographs in natura by the authors. A–C. L. bachmaensis sp. nov., Bach Ma National Park. A–B. ♀, 11 Jul. 2011. C. ♀, 14 Jul. 2011. D–G. L. bidoupensis sp. nov., Bidoup-Nui Ba National Park. D. ♂, 22 Jul. 2014. E–F. ♀, 25 Jul. 2014. G. ♀, 23 Jul. 2014. H–J. L. scheirei sp. nov. H–I. Dong Nai Biosphere Reserve, 30 Jun. 2012. H. ♂. I. ♀. J. ♀, Cat Tien National Park, 9 Jul. 2012.
Fig. 6 in The new genus of stick insect Lobofemora from Vietnam, with the description of three new species (Phasmida: Phasmatidae: Clitumnini)
Fig. 6. Lobofemora scheirei sp. nov. ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, lateral view. G. Apex of abdomen, ventral view. H. Anterior part of body, lateral view. D–H = not to scale.
Fig. 4 in The new genus of stick insect Lobofemora from Vietnam, with the description of three new species (Phasmida: Phasmatidae: Clitumnini)
Fig. 4. Lobofemora bidoupensis sp. nov. ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, lateral view. G. Apex of abdomen, ventral view. H. Anterior part of body, lateral view. D–H = not to scale.
Fig. 3 in The new genus of stick insect Lobofemora from Vietnam, with the description of three new species (Phasmida: Phasmatidae: Clitumnini)
Fig. 3. Lobofemora bachmaensis sp. nov. ♀, paratype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Right median leg, detail. E. Anterior part of body, dorsal view. F. Apex of abdomen, dorsal view. G. Apex of abdomen, lateral view. H. Apex of abdomen, ventral view. I. Anterior part of body, lateral view. D–I = not to scale.
Fig. 9 in The new genus of stick insect Lobofemora from Vietnam, with the description of three new species (Phasmida: Phasmatidae: Clitumnini)
Fig. 9. Lobofemora scheirei sp. nov., captive reared specimens (photographs by Dr B. Kneubühler). A–H. ♀. A. Dorsal view. B. Ventral view. C. Lateral view. D. Dorsolateral view, morph whitish with head, apex of abdomen and legs chestnut brown. E. Head, lateral view. F. Apex of abdomen, dorsal view. G. Apex of abdomen, lateral view. H. Apex of abdomen, ventral view. I–M. ♂. I. Dorsal. J. Wing spread, lateral view. K. Head and thorax, lateral view. L. Apex of abdomen, lateral view. M. Apex of abdomen, dorsal view. N. Nymph. O. Eggs. P–S. Egg. P. Detail of micropylar plate. Q. Ventral view. R. Detail of operculum. S. Detail of polar area. T. Eggs laid in a slice of sponge.
Fig. 10 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 10. Otraleus spp., photographs in natura. — A–F. O. christianae sp. nov. A. ♂, 16 Nov. 2013. B. ♀, 16 Nov. 2013. C. ♂, 15 Mar. 2015. D. ♂, nymph, 15 Mar. 2015. E–F. ♀, nymph, 24 Aug. 2015. — G–H. O. elizabethae sp. nov., ♀, 11 Apr. 2013 (photographs: A–F © A. Kang; G–H © T. Heitzmann).
Fig. 2 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 2. Otraleus applai sp. nov., ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 6 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 6. Otraleus bellemansae sp. nov., ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 5 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 5. Otraleus spp., photographs in natura. — A–B. O. applai sp. nov., ♀, 15 Apr. 2014. — C–H. O. bellemansae sp. nov. C. ♂, 15 Apr. 2014. D. ♀, 15 Apr. 2014. E. ♀, nymph, 23 Oct. 2013. F. ♀, nymph, 24 Oct. 2013. G. ♂, nymph, 15 Apr. 2014. H. ♀, nymph, 15 Apr. 2014 (photographs: A–C, H © A. Lefèvre; D–G © A. Kang).
Fig. 3 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 3. Otraleus applai sp. nov., ♀, paratype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 1 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 1. Otraleus hypsimelathrus Günther, 1935, ♂, holotype (ZMHB). A. Habitus, lateral view. B. Apex of abdomen, lateral view. C. Distribution map in Sulawesi. (Photographs © O. Conle and F. Hennemann.)
Fig. 16 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 16. Capuyanus magwilangi gen. et sp. nov., ♀, paratype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 15 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 15. Capuyanus magwilangi gen. et sp. nov., ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 12 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 12. Otraleus elizabethae sp. nov., ♀, paratype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 9 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 9. Otraleus christianae sp. nov., ♀, paratype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 11 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 11. Otraleus elizabethae sp. nov., ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 8 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 8. Otraleus christianae sp. nov., ♂, holotype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
Fig. 7 in Philippine mossy forest stick insects: first record of the genus Otraleus Günther, 1935 in the country, with four new species, and the new genus Capuyanus gen. nov. (Phasmida, Diapheromeridae, Necrosciinae)
Fig. 7. Otraleus bellemansae sp. nov., ♀, paratype. A. Habitus, dorsal view. B. Habitus, lateral view. C. Habitus, ventral view. D. Anterior part of body, dorsal view. E. Apex of abdomen, dorsal view. F. Apex of abdomen, ventral view. G. Apex of abdomen, lateral view. H. Anterior part of body, lateral view. D–H: not to scale.
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