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223 results for “Streptomyces”
Raw data for crystal structure of the flavoprotein monooxygenase TrlE from Streptomyces cyaneofuscatus Soc7, PDB ID: 8RQH
<p>X-Ray raw data for crystal structure of the flavoprotein monooxygenase TrlE from Streptomyces cyaneofuscatus Soc7 (PDB ID: 8RQH). The data have been collected at the Swiss Light Source (2022-11-20) at the X06SA beamline at a wavelength of 1.00003A.</p>
Plant community richness and foliar fungicides impact soil Streptomyces inhibition, resistance, and resource use phenotypes
Data associated with "Plant community richness and foliar fungicides impact soil Streptomyces inhibition, resistance, and resource use phenotypes" (DOI: 10.3389/fmicb.2024.1452534). These data include soil resource measurements and various phenotypic measurements of associated Streptomyces isolates/populations. Specifically, these data note population level inhibition phenotypes according to Herr's Assays, isolate level antibiotic resistance phenotypes against 9 standard antibiotics, and isolate level resource use phenotypes quantified with Biolog SF-P2 96 well plates.
Fig. 1 in Streptomyces aquilus sp. nov., a novel actinomycete isolated from a Chinese medicinal plant
Fig. 1. Optical micrograph (a) and scanning electron micrograph (b) of GGCR-6T grown on Gause's synthetic medium at 28 °C after incubation for 14 days.
Portada AyTBUAP 7(27). Representación del crecimiento de Streptomyces sp.
<p><strong>Resumen</strong></p> <p>La figura de la portada de Alianzas y Tendencias 2(27), muestra la representación del crecimiento de <em>Streptpmyces</em> sp.; una bacteria con la capacidad de producir compuestos antimicrobianos y compuestos de tipo anticancerígeno (1). Varias cepas del género <em>Streptomyces</em> se usan para el control biológico de microorganismos fitopatógenos, además de poseer capacidad promotora del crecimiento de plantas en los campos agrícolas (2), haciéndola un modelo de estudio importante para la generación de nuevo conocimiento (3). El modelamiento de microorganismos usando metodologías de arte digital puede hacer atractivo el entendimiento y despertar la imaginación de los lectores de diferentes disciplinas.</p>
Streptomyces Biosynthetic Gene Clusters
<p>A collection of genbank files 11975 biosynthetic gene clusters from generated using antiSMASH v6.0.1</p>
16S rRNA phylogeny and clustering is not a reliable proxy for genome-based taxonomy in Streptomyces
<p>This file is intended as supplementary information for a forthcoming publication: 16S rRNA phylogeny and clustering is not a reliable proxy for genome-based taxonomy in <em>Streptomyces</em>. </p>
Data from Nicolle et al. LC-HRMS study of Streptomyces sp. AgN23 Culture Media Extract. Study of AgN23 exometabolome and analysis of Arabidopsis metabolomic responses to the bacteria
<p>This archive compiles several datasets related to studies of <i>Streptomyces</i> sp. AgN23 interaction with <i>Arabidopsis thaliana</i>. Ultra-high-performance liquid chromatography-high-resolution MS (UHPLC-HRMS) analyses were performed on a Q Exactive Plus quadrupole (Orbitrap) mass spectrometer, equipped with a heated electrospray probe (HESI II) coupled to a U-HPLC Ultimate 3000 RSLC system (Thermo Fisher Scientific, Hemel Hempstead, United Kigdom). For each biological sample, the RAW file obtained in ESI+ and ESI- mode were retrieved from the Xcalibur version 4.4 software and are deposited in separate sub-folders termed "RawPos" and "RawNeg". Each experimental cohort is grouped in a folder where the biological repeats can be retrieved, as well as QC (Quality Check, pool of all samples from the cohort), Blank samples and eventual alternative control such as Bennett, the mock control media of <i>Streptomyces</i> sp. AgN23. The details regarding samples preparation, analytic parameters and mass spectrometry, statistical treatment and visualization of the data will be made available in the publication relating to this archive. The folder " AgN23-WT_AgN23-pSC004" contains chromatograms related to metabolomic study of Wild-type and pSC004-1, pSC004-10, pSC004-16 and pSC004-22 mutants of <i>Streptomyces</i> sp. AgN23. The folder " Col-0_AgN23" contains chromatograms related to metabolomic study of <i>Arabidopsis thaliana</i> Col-0 responses to colonization by <i>Streptomyces</i> sp. AgN23-WT. The folder " Col-0_pad3-1_AgN23" contains chromatograms related to metabolomic study of <i>Arabidopsis thaliana</i> Col-0 and the <i>Arabidopsis</i> pad3-1 mutant responses to colonization by <i>Streptomyces</i> sp. AgN23-WT. The folder " Col-0_pSC004" contains chromatograms related to metabolomic study of <i>Arabidopsis thaliana</i> Col-0 responses to colonization by <i>Streptomyces</i> sp. AgN23-WT and the AgN23 mutants pSC004-10 and pSC004-22. It should be noted that in the publication associated with this archive, the pSC004-1, pSC004-10, pSC004-16 and pSC004-22 mutants are referred to as ΔgbnB-1, ΔgbnB-2, ΔgbnB-3 and ΔgbnB-4, respectively.</p>
Streptomyces pathogen suppressive activity in plant communities varying in diversity:Plant host and plant diversity effects on rhizosphere microbial community composition, diversity, structure, and function.
Effects of plant host and plant community diversity on soil rhizosphere microbial community composition, diversity, structure, and function were explored in long-term experimental plots. Soil samples were collected from the rhizosphere of one of 4 target plants species (Andropogon gerardii, Schizachyrium scoparium, Lespedeza capitata, or Lupinus perennis) growing in 1, 4, 8, 16, or 32-species plots. Pathogen-suppressive activities of soil streptomycetes were determined for every sample. In addition, soil metagenomic analyses were performed targeting bacterial or streptomycete communities. Soil edaphic characteristics were determined for every sample. Analyses consider both the effects of plant host and plant community diversity on microbial community structure and function, and the relationships of diverse taxa with plant host, plant community diversity, and soil edaphic characteristics.
Fig. 3 in Streptomyces gossypiisoli sp. nov., isolated from cotton soil in Xinjiang, PR China
Fig. 3. Neighbour-joining phylogenetic tree based on concatenated partial sequences of the housekeeping genes atpD, gyrB, recA, rpoB and trpB. The relationships between strain TRM 44567T and the type strains of phylogenetically closely related species of the genus Streptomyces were analysed. *, Branches that were also found using the maximum-likelihood method; +, branches that were also found using the maximum-parsimony method; *+, branches that were found using all three methods. Numbers at nodes are percentage bootstrap values based on 1000 replicates; only values>50% are given. Bar, 0.0100 substitutions per nucleotide position.
Fig. 2 in Streptomyces gossypiisoli sp. nov., isolated from cotton soil in Xinjiang, PR China
Fig. 2. Neighbour-joining phylogenetic tree based on nearly complete 16S rRNA gene sequences. The relationships between strain TRM 44567T and the type strains of phylogenetically closely related species of the genus Streptomyces were analysed. Actinomadura hibisca JCM 9627T (AF163115) was used as the outgroup. *, Branches that were also found using the maximum- likelihood method; +, branches that were also found using the maximum-parsimony method; *+, branches that were found using all three methods. Numbers at nodes are percentage bootstrap values based on 1000 replicates; only values>50% are given. Bar, 0.0100 substitutions per nucleotide position.
Fig. 1 in Streptomyces gossypiisoli sp. nov., isolated from cotton soil in Xinjiang, PR China
Fig. 1. Scanning electron microscopy image of strain TRM 44567T grown on Gause's agar at 37 °C for 7 days. Bars, 5 µm (left) and 20 µm (right).
Fig. 3 in Streptomyces reniochalinae sp. nov. and Streptomyces diacarni sp. nov., from marine sponges
Fig. 3. Neighbour-joining tree based on five-gene concatenated sequences (atpD, gyrB, recA, rpoB and trpB, 2454 nt) showing the relationships between strains LHW50302T, LHW51701T and related members of the genus Streptomyces. Kitasatospora aburaviensis NRRL B-2218T was used as an outgroup. Numbers at nodes indicate levels of bootstrap support (%) based on neighbour-joining analysis of 1000 resampled datasets; only values above 50 % are shown. Asterisks (*) and crosses (‡) indicate branches that were also found using the maximum-likelihood method and the maximum-parsimony method, respectively. Bar, 0.02 substitutions per site.
Fig. 2 in Streptomyces reniochalinae sp. nov. and Streptomyces diacarni sp. nov., from marine sponges
Fig. 2. Neighbour-joining phylogenetic tree, based on almost-complete 16S rRNA gene sequences, showing the phylogenetic relationships of strains LHW63021T, LHW51701T and related members of the genus Streptomyces. Kitasatospora aburaviensis NRRL B-2218T was used as an outgroup. Numbers at nodes indicate levels of bootstrap support (%) based on neighbour-joining analysis of 1000 resampled datasets; only values above 50 % are shown. Asterisks (*) and crosses (‡) indicate branches that were also found using maximum-likelihood method and the maximum-parsimony method, respectively. Bar, 0.005 substitutions per nucleotide position.
Fig. 1 in Streptomyces reniochalinae sp. nov. and Streptomyces diacarni sp. nov., from marine sponges
Fig. 1. Scanning electron micrograph of strain LHW50302T (a) and strain LHW51701T (b) showing a hooked and looped spore arrangement and smooth spore ornamentation following growth on ISP 2 at 28 ǪC for 7 days. Bar, 1 µm.
Fig. 2 in Streptomyces krungchingensis sp. nov., isolated from soil
Fig. 2. Phylogenetic relationships based on an NJ analysis of 16S rRNA gene sequences of strain KC-035T and closely related Streptomyces species. Kitasatospora setae JCM 3304T was used as the outgroup. Symbols indicate that branches were also recovered in the ML (*) and MP (#) trees. The numbers at branch nodes indicate bootstrap percentages derived from 1000 replications (only values>50 % are shown). Bar, 0.005 substitutions per nucleotide position.
Fig. 1 in Streptomyces krungchingensis sp. nov., isolated from soil
Fig. 1. Scanning electron micrograph showing the flexuous spore chains and smooth spore surface of strain KC-035T after growing on ISP2 medium at 30 ǪC for 14 days. Bar, 1 µm.
Fig. 1 in Streptomyces ciscaucasicus Sveshnikova et al. 1983 is a later subjective synonym of Streptomyces canus Heinemann et al. 1953
Fig. 1. Phylogenetic relationship of Streptomyces canus DSM 40017T and Streptomyces ciscaucasicus DSM 40275T to each other and to the next closest related type strains in phylogenetic trees calculated based on nucleotide (a) and amino acid (b) sequences of shared protein coding genes and concatenated nucleotide (c) and amino acid sequences (d) used for MLSA (atpD–gyrB–recA–rpoB–trpB). For the genome-based analyses the data were validated by bootstrap analysis with 200 iterations; all branches showed at least 95 % bootstrap support. MLSA trees were based on 100 replications (bootstrap analysis). Genome accession numbers are listed in Table S1. Bars, 0.01 nucleotide or amino acid sequence exchange per sequence position.
Commercial standards of Streptomyces antibiotics (LC-MS-MS/MS)
<p>Commercial standards for 8 antibiotics produced by Streptomyces used for method validation for the untargeted metabolomics workflow UmetaFlow. Germicidin A, Germicidin B, Kanamycin, Tetracycline, Thiostreptone, Globomycin, Ampicillin, Apramycin. Publicly available also at MassIVE MSV000090047.</p>
LC-HRMS study of Arabidopsis root metabolome in wild-type Col-0 and npr1 mutant upon in vitro inoculation with Streptomyces sp. AgN23.
<p>This archive compiles datasets related to studies of <em>Streptomyces</em> sp. AgN23 interaction with <em>Arabidopsis thaliana</em>. Ultra-high-performance liquid chromatography-high-resolution MS (UHPLC-HRMS) analyses were performed on a Q Exactive Plus quadrupole (Orbitrap) mass spectrometer, equipped with a heated electrospray probe (HESI II) coupled to a U-HPLC Ultimate 3000 RSLC system (Thermo Fisher Scientific, Hemel Hempstead, United Kigdom). For each biological sample, the RAW file obtained in ESI+ and ESI- mode were retrieved from the Xcalibur version 4.4 software and are deposited in separate sub-folders termed "RawPos" and "RawNeg". Each folder contains all the data relative to the cohort comprising "Blank" samples (n=13), Quality Check samples (pool of all samples from the cohort) "QC" (n=8), Control Col-0 plant samples "Col0CTRL" (n=6), Col-0 plant inoculated with AgN23 samples "Col0AgN23WT" (n=6), Control npr1 plant samples"NPR1CTRL" (n=6), npr1 plant inoculated with AgN23 samples "NPR1AgN23WT" (n=6). The files belonging to Negative mode bare the "neg" suffix and those belonging to Positive mode "pos" suffix, i.e. "Col0AgN23WT1_neg" and Col0AgN23WT1_pos". The details regarding samples preparation, analytic parameters and mass spectrometry, statistical treatment and visualization of the data will be made available in the publication relating to this archive. </p>
Fig. 5 Insect-associated Streptomyces are a in The antimicrobial potential from insect microbiomes of Streptomyces
Fig. 5 Insect-associated Streptomyces are a source of active antimicrobials. a Fractionated extracts from insect microbiomes are active in multiple murine models of drug-resistant infection. Less infective burden is seen in intraperitoneally treated mice after 8 h of infection. Each dot represents a unique fraction in one mouse study. (n = 15, 11, and 8 for C. albicans, E. coli, and P. aeruginosa models, respectively; center, median; box, upper and lower quantiles; whiskers, 1.5× interquartile range. b Most fractions from insect microbiomes show no hemolysis in cell-based assays. Safe indicates no toxicity at>100× concentration associated with efficacy. c The antifungal cyphomycin is produced by Streptomyces isolated from d the fungus-growing ant Cyphomyrmex sp. Photo credit: Alexander Wild e Cyphomycin-containing fractions show potency against the ant pathogen Escovopsis sp. (top left, bottom). f Purified cyphomycin exhibits potency against resistant pathogens. g Mouse candidiasis (C. albicans) models showcase reduced infection and a dose-like response to cyphomycin. Dots indicate individual mice
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
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OpenNeuro
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