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4 results for “Strix occidentalis”
Supplemental dataset for Northern Spotted Owl (<i>Strix occidentalis caurina</i>) genome assembly version 1.0
<p><strong>StrOccCau_1.0_nuc.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the file that we deposited at DDBJ/ENA/GenBank as a Whole Genome Shotgun (WGS) project under accession NIFN00000000. It is is the file that you will most likely want to download if you would like to perform an alignment to this genome assembly. This file is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt and also without the contigs and scaffolds that we identified either as the mitochondrial genome sequence or as contaminant sequences.</p> <p><strong>StrOccCau_1.0_nuc_masked.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the repeat-masked (hard-masked) assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt and also without the contigs and scaffolds that we identified either as the mitochondrial genome sequence or as contaminant sequences.</p> <p><strong>StrOccCau_1.0_mito.fa</strong> : This FASTA format file is the mitochondrial-genome-derived scaffold from the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012).</p> <p><strong>StrOccCau_1.0.gff.bz2</strong> : This gff format file compressed this file with bzip2 contains the gene annotations of StrOccCau_1.0_nuc.fa.</p> <p><strong>StrOccCau_1.0_transcripts.fa.bz2</strong> : This FASTA format file compressed this file with bzip2 contains the sequences of the gene transcript sequences of the genes annotated in StrOccCau_1.0.gff.</p> <p><strong>StrOccCau_1.0_proteins.fa.bz2</strong> : This FASTA format file compressed this file with bzip2 contains the protein sequences of the genes annotated in StrOccCau_1.0.gff.</p> <p><strong>StrOccCau_1.0_RM_homology_includes_LowComplexity.out.bz2</strong> : This file provides the repeat annotations produced by the homology-based masking of StrOccCau_1.0_nuc.fa that included masking of low complexity regions and simple repeats. We compressed this file with bzip2.</p> <p><strong>StrOccCau_1.0_RM_DeNovo_includes_LowComplexity.out</strong> : This file provides the repeat annotations produced by the de novo masking (which followed after first performing homology-based masking) of StrOccCau_1.0_nuc.fa that included masking of low complexity regions and simple repeats.</p> <p><strong>StrOccCau_1.0_RM_homology_no_LowComplexity.out.bz2</strong> : This file provides the repeat annotations produced by the homology-based masking of StrOccCau_1.0_nuc.fa that did not include masking of low complexity regions and simple repeats. We compressed this file with bzip2.</p> <p><strong>StrOccCau_1.0_RM_DeNovo_no_LowComplexity.out</strong> : This file provides the repeat annotations produced by the de novo masking (which followed after first performing homology-based masking) of StrOccCau_1.0_nuc.fa that did not include masking of low complexity regions and simple repeats.</p> <p><strong>StrOccCau_1.0_alignments_of_light_associated_genes.txt</strong> : This file provides alignments of light-associated gene orthologs as well as assemblies of transcriptome sequences in NEXUS format.</p> <p><strong>StrOccCau_1.0_nuc_masked_SpottedBarredOwl_variant_file.vcf.bz2</strong> : This is a raw, unfiltered variant call format file compressed with bzip2 that was generated after aligning both spotted owl and barred owl short read data aligned to StrOccCau_1.0_nuc_masked.fa.</p> <p><strong>StrOccCau_0.1.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012).</p> <p><strong>StrOccCau_0.1_masked.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the repeat-masked assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012).</p> <p><strong>StrOccCau_0.2.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt.</p> <p><strong>StrOccCau_0.2_masked.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the repeat-masked assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without any contigs and scaffolds less than 1,000 nt.</p> <p><strong>StrOccCau_GapCloser_output_NoContamNoMito.fa.bz2</strong> : This FASTA format file compressed with bzip2 is the assembly output from SOAPdenovo2 toolkit GapCloser version 1.12-r6 (Luo et al. 2012) without the contigs and scaffolds that we later identified either as the mitochondrial genome sequence or as contaminant sequences.</p> <p><strong>Citations</strong> - if you utilize these data, please include these citations:</p> <p>Hanna ZR., Henderson JB., Wall JD., Emerling CA., Fuchs J., Runckel C., Mindell DP., Bowie RCK., DeRisi JL., Dumbacher JP. 2017a. Supplemental dataset for Northern Spotted Owl (<em>Strix occidentalis caurina</em>) genome assembly version 1.0. <em>Zenodo</em>. DOI: 10.5281/zenodo.822859.</p> <p>Hanna ZR., Henderson JB., Wall JD., Emerling CA., Fuchs J., Runckel C., Mindell DP., Bowie RCK., DeRisi JL., Dumbacher JP. 2017b. Northern Spotted Owl (Strix occidentalis caurina) Genome: Divergence with the Barred Owl (<em>Strix varia</em>) and Characterization of Light-Associated Genes. <em>Genome Biology and Evolution</em> 9:2522–2545. DOI: 10.1093/gbe/evx158.</p>
Adapter sequences used for trimming of genomic sequences in the assembly of the Northern Spotted Owl (<i>Strix occidentalis caurina</i>) genome assembly version 1.0
<p>These files provide the sequences of the adapters used in the construction of the genomic libraries Hanna et al. (2017a) sequenced and used to assemble the Northern Spotted Owl (<em>Strix occidentalis caurina</em>) genome assembly version 1.0 (Hanna et al. 2017b). These files also contain relevant supplemental adapter sequences from the adapter files included with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011595_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011595. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011596_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011596. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011597_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011597. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011614_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011614. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" and "NexteraPE-PE.fa" files distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011615_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011615. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" file distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p> <p><strong>SRR4011616_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011616. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" file distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).<br> <br> <strong>SRR4011617_adapters.fa</strong> : This FASTA format file contains the full length sequences of the adapters Hanna et al. (2017a) used to construct the genomic library they sequenced to produced the data uploaded as NCBI Sequence Read Archive (SRA) run accession SRR4011617. I have also included the partial adapter sequences provided in the "TruSeq3-PE-2.fa" file distributed with Trimmomatic version 0.36 (Bolger, Lohse & Usadel, 2014).</p>
Repeat-masked <i>Strix occidentalis caurina</i> nuclear genome version 1.0 and complete mitochondrial genome
<p><strong>StrOccCau_1.0_nuc_finalMito_RepeatMasked.fa.bz2</strong> : This file is homology-based and <em>de novo</em> model-based repeat-masking of the reference <em>Strix occidentalis caurina</em> genome StrOccCau_1.0_nuc.fa from Hanna et al., 2017a,b) with the mitochondrial genome from Hanna et al. (2017c).</p> <p><strong>StrOccCau_1.0_nuc_finalMito_RepeatMasked.bed.bgz</strong> : This is a file in Browser Extensible Data (BED) format that provides the genomic intervals of the N-regions in the above masked assembly. The N-regions include hard-masked low complexity and repeat regions as well as N-regions that were gaps in the original assembly. I compressed the file using the bgzip tool from HTSlib version 1.7 (Davies et al. 2018).</p> <p>See full details of the creation of these files in section 2.1 of the materials and methods at protocols.io (http://dx.doi.org/10.17504/protocols.io.rmrd456).</p> <p>If you use these data, please cite the following:</p> <p>Hanna ZR. 2018. Repeat-masked <em>Strix occidentalis caurina</em> nuclear genome version 1.0 and complete mitochondrial genome. Version 1.0.0. <em>Zenodo.</em></p> <p> </p> <p><strong>References</strong></p> <p>Davies R, Randall JC, McCarthy SA, Bonfield J, Pollard MO, Marshall J, et al. 2018. HTSlib. Version 1.7. [Accessed 2018 Mar 19]. Available from: https://github.com/samtools/htslib</p> <p>Hanna ZR, Henderson JB, Wall JD, Emerling CA, Fuchs J, Runckel C, et al. 2017a. Northern Spotted Owl (<em>Strix occidentalis caurina</em>) Genome: Divergence with the Barred Owl (<em>Strix varia</em>) and Characterization of Light-Associated Genes. Genome Biology and Evolution. 9: 2522–2545. DOI: 10.1093/gbe/evx158</p> <p>Hanna ZR, Henderson JB, Wall JD, Emerling CA, Fuchs J, Runckel C, et al. 2017b. Supplemental dataset for Northern Spotted Owl (<em>Strix occidentalis caurina</em>) genome assembly version 1.0. <em>Zenodo</em>. DOI: 10.5281/zenodo.822859</p> <p>Hanna ZR, Henderson JB, Sellas AB, Fuchs J, Bowie RCK, Dumbacher JP. 2017c. Complete mitochondrial genome sequences of the northern spotted owl (<em>Strix occidentalis caurina</em>) and the barred owl (<em>Strix varia</em>; Aves: Strigiformes: Strigidae) confirm the presence of a duplicated control region. <em>PeerJ</em>. 5: e3901. DOI: 10.7717/peerj.3901</p> <p> </p>
Gene annotations for <i>Strix occidentalis caurina</i> draft nuclear genome assembly version 1
<p><strong>NSO-wgs-v1-nuc.gff.bz</strong> : This file provides the gene annotations produced by the MAKER pipeline for the <em>Strix occidentalis caurina</em> draft whole nuclear genome assembly, NSO-wgs-v1-nuc.</p> <p><strong>NSO-wgs-v1-nuc-masked-homology-includes-LowComplexity.out.bz</strong> : This file provides the repeat annotations produced by the homology-based masking of NSO-wgs-v1-nuc that included masking of low complexity regions and simple repeats.</p> <p><strong>NSO-wgs-v1-nuc-masked-DeNovo-includes-LowComplexity.out</strong> : This file provides the repeat annotations produced by the <em>de novo</em> masking of NSO-wgs-v1-nuc that included masking of low complexity regions and simple repeats.</p> <p><strong>NSO-wgs-v1-nuc-masked-homology-no-LowComplexity.out.bz</strong> : This file provides the repeat annotations produced by the homology-based masking of NSO-wgs-v1-nuc that did not include masking of low complexity regions and simple repeats.</p> <p><strong>NSO-wgs-v1-nuc-masked-DeNovo-no-LowComplexity.out</strong> : This file provides the repeat annotations produced by the <em>de novo</em> masking of NSO-wgs-v1-nuc that did not include masking of low complexity regions and simple repeats.</p>
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