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Dataset results
13 results for “Sugar Signaling”
Data from: Transcriptome analysis of apical meristem enriched bud samples for size dependent flowering commitment in Crocus sativus reveal role of sugar and auxin signalling
<p><strong>Background</strong></p> <p>Cultivation of <em>Crocus sativus</em> (saffron) faces challenges due to inconsistent flowering patterns and variations in yield. Flowering takes place in a graded way with smaller corms unable to produce flowers. Enhancing the productivity requires a comprehensive understanding of the underlying genetic mechanisms that govern this size based flowering initiation and commitment. Therefore, samples enriched with non-flowering and flowering apical buds from small (<6g) and large (>14g) corms were sequenced. </p> <p><strong>Methods and Results</strong></p> <p>Apical bud enriched samples from small and large corms were collected immediately after break of dormancy in July. RNA sequencing was performed using Illumina Novaseq 6000. <em>De-novo</em> transcriptome assembly and analysis using flowering committed buds from large corms at post-dormancy and their comparison with vegetative shoot primordia from small corms pointed out the major role of Auxin and ABA hormonal regulation. Many genes with known dual responses in flowering development and circadian rhythm like Flowering locus T and Cryptochrome 1 along with a transcript showing homology with small auxin upregulated RNA (SAUR) exhibited induced expression in flowering buds. Thorough prediction of <em>Crocus sativus</em> non-coding RNA repertoire has been carried out for the first time. Enolase was found to be acting as a major hub with protein-protein interaction analysis using Arabidopsis counterparts.</p> <p><strong>Conclusion</strong></p> <p>Transcripts belong to key pathways including phenylpropanoid biosynthesis, hormone signaling and carbon metabolism were found significantly modulated. KEGG assessment and protein-protein interaction analysis confirm the expression data. Findings unravel the genetic determinants driving the size-dependent flowering in <em>Crocus sativus</em>.</p>
Sugar signal manipulation by T6P for yield on wheat grain - whole grain RNA-seq
<p>Trehalose 6-phosphate (T6P) is a powerful internal sugar signal in plants yet cannot be directly added nor<br>fully genetically controlled. A timed microdose of a plant-permeable T6P signalling precursor, DMNB-T6P, causes substantial yield improvements. This repository contains normalised counts table for a RNA-seq of wheat (Triticum aestivum) whole grains after spray with mock/DMNB-T6P.</p> <p>DESeq2 normalised count table for raw reads deposited in ncbi SRA under Bioproject PRJNA1007614. Related manuscript in press in Nature Biotechnology.</p> <p> </p> <p>Methods extract below:</p> <p>(...) Following RNA integrity analysis and quantitation (Bioanalyser; Agilent, USA), poly-A enriched cDNA libraries were generated and sequenced on an Illumina Novaseq 6000 sequencing platform generating 30–50 million 150 bp paired-end reads per sample. Low-quality reads and adaptor sequences were removed with Trimmomatic (trimmomatic-0.39.jar PE ILLUMINACLIP:TruSeq3-PE.fa:2:30:10:2:True TRAILING:30 MINLEN:40). The reads were aligned to the wheat reference genome (<em>Triticum aestivum</em> iwgsc_refseqv2.1 using HISAT2/2.2.1-foss-2019b with default parameters and converted to Bam format with SAMtools. Gene abundance was quantified using featureCounts with the High Confidence iwgsc_refseqv2.1 annotation (counting only primary alignments of read pairs with a quality cut-off of 10). Raw counts were normalized using the trimmed mean of M-values method by DESeq2.</p>
RNA-seq of Arabidopsis seedlings treated with chemical modifiers of sugar signalling at dawn
GEO Series GSE188596. Arabidopsis thaliana. 18 samples. Type: Expression profiling by high throughput sequencing.
The TaSnRK1-TabHLH489 module integrates brassinosteroid and sugar signaling to regulate grain length in bread wheat
GEO Series GSE253343. Triticum aestivum. 6 samples. Type: Expression profiling by high throughput sequencing.
Transforming growth factor β/activin signaling functions as a sugar-sensing feedback loop to regulate digestive enzyme expression.
GEO Series GSE54755. Drosophila melanogaster. 9 samples. Type: Expression profiling by high throughput sequencing.
Sugar activates defense signaling in Arabidopsis
GEO Series GSE169473. Arabidopsis thaliana. 36 samples. Type: Expression profiling by high throughput sequencing.
Resveratrol improves adipose insulin signaling and reduces the inflammatory response in adipose tissue of rhesus monkeys on a high-fat, high-sugar diet.
GEO Series GSE50005. Macaca mulatta; Homo sapiens. 45 samples. Type: Expression profiling by array.
Clone-specific transcript profiling of ‘Nebbiolo’ grape berries unveils environmental responses mediated by sugar and secondary metabolite signalling
GEO Series GSE116238. Vitis vinifera. 81 samples. Type: Expression profiling by high throughput sequencing.
The vacuolar sugar transporter EARLY RESPONSE TO DEHYDRATION 6-LIKE4 regulates fructose signaling and plant growth
GEO Series GSE222562. Arabidopsis thaliana. 18 samples. Type: Expression profiling by high throughput sequencing.
bzip9-Energetic Homeostasis and Sugar Signaling
GEO Series GSE26041. Arabidopsis thaliana. 24 samples. Type: Expression profiling by array.
Expression Profile of Signal Transduction Components in a Sugarcane Population Segregated for Sugar Content
GEO Series GSE4233. Saccharum officinarum. 4 samples. Type: Expression profiling by array.
cordycepin-Energetic Homeostasis and Sugar Signaling
GEO Series GSE26045. Arabidopsis thaliana. 24 samples. Type: Expression profiling by array.
abi4-Energetic Homeostasis and Sugar Signaling
GEO Series GSE26044. Arabidopsis thaliana. 24 samples. Type: Expression profiling by array.
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