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Dataset results
111 results for “T47D”
A bioactive compound isolated from Duku (Lansium domesticum Corr) fruit peels exhibits cytotoxicity against T47D cell line
<p><strong>Background: </strong>Breast cancer is a major health problem for women globally. Many attempts have been promoted to cure cancer by finding new anticancer medicines from natural resources. Despite the richness of biodiversity discovered, there are some natural resources that remain unexplored. Fruit peels of Duku (<em>Lansium domesticum</em> Corr.) are rich with compounds that may have the potential to be developed as anticancer drugs. This study aimed to isolate cytotoxic compounds from the fruit peels of <em>L. domesticum</em> and assess their cytotoxic nature against T47D cells.</p> <p><strong>Methods: </strong>Powdered peels were macerated with ethyl acetate and the filtrate was evaporated to give EtOAc extract A. Dried extract A was triturated with n-hexane to give n-hexane soluble fraction B and insoluble fraction C. The cytotoxic nature of these three samples were assessed using MTT assay using T47D cells and doxorubicin as a control</p> <p><strong>Results: </strong>Fraction C that showed the smallest IC<sub>50</sub> (25.56 + 0.64µg/mL) value compared to extract A and fraction BFraction C was further fractionated by vacuum liquid chromatography to give 6 subfractions. Subfraction 2 showed a single compound based on thin layer chromatography, and this compound was identified as Lamesticumin A on the basis of its spectroscopic data. Lamesticumin A demonstrated cytotoxic activity against T47D cell lines with an IC<sub>50</sub> value of 15.68 + 0.30µg/mL.</p>
Transcriptome analysis of T47D cells and H2A.J-KO derivatives for the paper entitled: The histone variant H2A.J is enriched in luminal epithelial gland cells
<p>H2A.J is a poorly studied mammalian-specific variant of histone H2A. We used immunohistochemistry to study its localization in various human and mouse tissues. H2A.J showed cell-type specific expression with a striking enrichment in luminal epithelial cells of multiple glands including those of breast, prostate, pancreas, thyroid, stomach, and salivary glands. H2A.J was also highly expressed in many carcinoma cell lines and in particular, those derived from luminal breast and prostate cancer. H2A.J thus appears to be a novel marker for luminal epithelial cancers. Knocking-out the H2AFJ gene in T47D luminal breast cancer cells reduced the expression of several estrogen-responsive genes which may explain its putative tumorigenic role in luminal-B breast cancer.</p>
Cytotoxic activity of betulinic acid and ursolic acid againts T47D breast cancer cells
<p><em><span>Uncaria nervosa</span></em><span> Elmer is an Indonesian herbal plant that is traditionally used for breast cancer. The results of phytochemical screening contained alkaloids, flavonoids, and terpenoids in the ethanol extract of this plant. Based on literature searches, reports regarding the bioactive compounds responsible for breast cancer have not been found. </span><span>This study aims to determine the metabolite profiling of ethanol extract, the isolation, characterization of bioactive compounds, and their bioactivity in T47D breast cancer cells. The ethanol extract of <em>Uncaria nervosa</em> Elmer leaves contains nine compounds consisting of alkaloids, terpenoids, and fatty acid. The bioactive compounds that were successfully isolated were betulinic acid, and ursolic acid, with IC50 values of ˃100 and 14,70±4,50 µg/ml, respectively.</span></p>
Data from: Metabolites of n-Butylparaben and iso-Butylparaben exhibit estrogenic properties in MCF-7 and T47D human breast cancer cell lines
Two oxidized metabolites of n-butylparaben (BuP) and iso-butylparaben (IsoBuP) discovered in human urine samples exhibit structural similarity to endogenous estrogens. We hypothesized that these metabolites bind to the human estrogen receptor (ER) and promote estrogen signaling. We tested this using models of ER-mediated cellular proliferation. The estrogenic properties of 3-hydroxy n-butyl 4-hydroxybenzoate (3OH) and 2-hydroxy iso-butyl 4-hydroxybenzoate (2OH) were determined using the ER-positive, estrogen-dependent human breast cancer cell lines MCF-7, and T47D. The 3OH metabolite induced cellular proliferation with EC50 of 8.2 µM in MCF-7 cells. The EC50 for 3OH in T47D cells could not be reached. The 2OH metabolite induced proliferation with EC50 of 2.2 µM and 43.0 µM in MCF-7 and T47D cells, respectively. The EC50 for the parental IsoBuP and BuP was 0.30 and 1.2 µM in MCF-7 cells, respectively. The expression of a pro-proliferative, estrogen-inducible gene (GREB1) was induced by these compounds and blocked by co-administration of an ER antagonist (ICI 182, 780), confirming the ER-dependence of these effects. The metabolites promoted significant ER-dependent transcriptional activity of an ERE-luciferase reporter construct at 10 and 20 µM for 2OH and 10 µM for 3OH. Computational docking studies showed that the paraben compounds exhibited the potential for favorable ligand-binding domain interactions with human ERα in a manner similar to known x-ray crystal structures of 17ß-estradiol in complex with ERα. We conclude that the hydroxylated metabolites of BuP and IsoBuP are weak estrogens and should be considered as additional components of potential endocrine disrupting effects upon paraben exposure.
InfoHiC models for cancer Hi-C prediction (T47D, 1Mb window, 40kb resolution)
<p>InfoHiC models for cancer Hi-C prediction</p> <ul> <li>T47D</li> <li>1Mb window</li> <li>40kb resolution</li> <li>CSCN encoding</li> </ul>
InfoHiC dataset of T47D chromosomes 8 and 14
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InfoHiC dataset of T47D HiC reads
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InfoHiC T47D
<p>InfoHiC T47D data (Hi-C matrix)</p>
InfoGenomeR T47D
<p>T47D data for InfoGenomeR</p>
Data from: Metabolites of n-Butylparaben and iso-Butylparaben exhibit estrogenic properties in MCF-7 and T47D human breast cancer cell lines
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Expression profile of MCF7 and T47D human breast cancer cell lines upon treatment with the BET bromodomian inhibitor JQ1
GEO Series GSE95287. Homo sapiens. 12 samples. Type: Expression profiling by array.
Hypoxia-related transcription factor ChIP-seq and EglN2 knockdown expression profiling in T47D breast cancer cells
GEO Series GSE59937. Homo sapiens. 9 samples. Type: Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing.
aCGH hybridization of breast cancer cell line T47D
GEO Series GSE22547. Homo sapiens. 1 samples. Type: Genome variation profiling by genome tiling array.
Gene expression by high-throughput sequencing of T47D-MTVL human breast cancer cells upon H1.4 knock-down and multiple H1 variants
GEO Series GSE83277. Homo sapiens. 7 samples. Type: Expression profiling by high throughput sequencing.
RNA-seq analysis of ESR1 mutations in T47D and MCF7 cell lines
GEO Series GSE89888. Homo sapiens. 48 samples. Type: Expression profiling by high throughput sequencing.
Mapping of somatic linker histone H1 variants in T47D-MTVL and HeLa cells
GEO Series GSE49333. Homo sapiens. 10 samples. Type: Genome binding/occupancy profiling by genome tiling array.
polyA mRNA RNA-seq from T47D (ENCSR000BYE)
GEO Series GSE93442. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Hypoxia-related Transcription factor ChIP-Seq data in T47D breast cancer cells
GEO Series GSE59935. Homo sapiens. 5 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Effects of HOTAIR RNAi on Transcriptomes in T47D Cells
GEO Series GSE119511. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Estrogen Receptor Activation Time Course at 3, 6, 12 & 24h in MCF7, T47D and MDA-MB-231 with and without ZMIZ1 knockdown
GEO Series GSE133381. Homo sapiens. 286 samples. Type: Expression profiling by high throughput sequencing.
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