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28 results for “TCA cycle”
Fig. 6 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development
Fig. 6 Phenotype associated with dynein, kinesin, isocitrate dehydrogenase and citrate synthase mRNA subjected to RNAi in female ticks via injection with the corresponding dsRNA. a Dynein dsRNA injection. b Kinesin dsRNA injection. c Isocitrate dehydrogenase dsRNA injection. d Citrate synthase dsRNA injection. e GFP dsRNA injection, control. f No injection, control. Scale-bars: 5 mm
Fig. 7 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development
Fig. 7 Digital micrographs of salivary gland acinar morphological changes in unfed female H. longicornis after RNAi.The time at which the tick bit the host and began sucking blood was recorded as day 0. a–e Dynein dsRNA injection. f–j Kinesin dsRNA injection. k–o Isocitrate dehydrogenase dsRNA injection. p–t Citrate synthase dsRNA injection. u–y GFP dsRNA injection, control. Scale-bars: 25 µm
Fig. 4 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development
Fig. 4 KEGG pathway enrichment analysis of the differentially expressed proteins in 5 different Clusters. Terms with a P-value <0.05 were used to draw the column diagrams. a–e KEGG pathway enrichment for the proteins in Cluster 1 to Cluster 5
Fig. 3 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development
Fig. 3 GO functional annotations for all the differentially expressed proteins. a–c GO annotations of differentially expressed proteins in the salivary glands of partially fed ticks compared with unfed ticks (115:114). d–f GO annotations of differentially expressed proteins in the salivary glands of mated semi-engorged ticks compared with partially fed ticks (116:115). g–i GO annotations of differentially expressed proteins in the salivary glands of engorged ticks compared with mated semi-engorged ticks (117:116). Abbreviations: BP, biological process; CC, cellular component; MF, molecular function; CO, cellular component organization or biogenesis
Fig. 5 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development
Fig. 5 RT-qPCR analyzed the mRNA expression levels of dynein, kinesin, isocitrate dehydrogenase, and citrate synthase during the four feeding stages of salivary gland development
Fig. 2 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development
Fig. 2 Statistics and cluster analysis for the identified proteins and their expression levels in the salivary glands of female H. longicornis. a Venn diagram showing the number of proteins (with CV <20%) identified in the three experiments. b Venn diagram showing the number of proteins with quantitative information. c Cluster analysis according to trends in protein expression in the salivary glands of female ticks
Fig. 1 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development
Fig. 1 Workflow for quantitative proteomics analysis of changes in protein expression in the salivary glands of female H. longicornis during the blood-feeding process
Quantification of phosphorylated metabolites, organic acids, and intermediates of the TCA cycle using capillary ion chromatography tandem mass spectrometry (capIC-MS/MS) following treatment of Escherichia coli with ciprofloxacin
<p>Capillary ion chromatography tandem mass spectrometry (capIC-MS/MS) was used to quantify phosphorylated metabolites, organic acids, and intermediates of the TCA cycle of Escherichia coli treated with ciprofloxacin, BTP-001 (a novel antimicrobial peptide), and a combination of the two . Metabolite extracts were analyzed with a Xevo TQ-XS triple quadrupole mass spectrometer (Waters, USA).</p><p>Samples were gathered from E. coli cultures grown in batch cultivations using 1 liter bioreactors. Briefly, intracellular metabolites were extracted by cycling samples between −20 °C EtOH and N2 (<i>l</i>) in three consecutive freeze–thaw cycles, with vortexing every 10 min during the thawing phase. Filters were removed and the cell debris was pelleted (4500 rcf, 10 min, -9 °C). The supernatants were transferred to a new tube, snap frozen in N2 (<i>l</i>), and lyophilized. Lyophilized extracts were reconstituted in 500 µL cold Milli-Q H2O and cleared by spin-filtration with a 10 kDa molecular cutoff (20817 rcf, 10 min, 0 °C). A mix of 80 µL centrifuged sample and 20 µL 13C-labeled ISTD extract from yeast was sent to analysis. </p><p>Data processing and absolute quantification was performed as earlier described using the TargetLynx application manager of MassLynx v 4.1 (Waters) to interpolate calibration curves made with appropriate dilutions of analytical grade standards (Sigma-Aldrich). The response factor of the corresponding U13C-isotopologues were used to correct the standard and sample extract response factors. Extract concentrations were normalized to the CDW, which was calculated from interpolation of the OD600 vs. CDW (g/L) curve. </p><p>Further statistical analysis in MetaboAnalyst v 5.0 replaced missing values with 1/5 of the minimum value of the respective metabolite. An unpaired T-test with unequal variance determined differential enriched metabolites with a false discovery rate (FDR) < 0.05 which are presented as log2 fold-change compared to control. </p>
Expanded TCA cycle (TriCarboxylic Acid Cycle, aka Citric Acid Cycle, aka Krebs cycle)
<p>Major metabolic pathways feeding into and out of the TCA cycle (TriCarboxylic Acid Cycle, aka Citric Acid Cycle, aka Krebs cycle)</p>
Disruption of the TCA cycle reveals an ATF4-mediated integration of redox and amino acid metabolism
<p>The Tricarboxylic Acid (TCA) cycle is arguably the most critical metabolic cycle in physiology and exists as an essential interface coordinating cellular metabolism, bioenergetics, and redox homeostasis. Despite decades of research, a comprehensive investigation into the consequences of TCA cycle dysfunction remains elusive. Here, we targeted two TCA cycle enzymes, fumarate hydratase (FH), using an FH inhibitor (FHIN-1), and succinate dehydrogenase (SDH), using two inhibitors Atpenin A5 (AA5) and thenoyltrifluoroacetone (TTFA), and combined transcriptomics, and proteomics analyses to fully appraise the consequences of TCA cycle inhibition (TCAi) in murine kidney epithelial cell line.</p> <p>Data from this study that is included in this Dryad submission is as follows:</p> <p>1. TruSeq mRNA stranded analysis of murine Fh1<sup>fl/fl</sup> kidney epithelial cells were treated with vehicle control (DMSO) or 20 micromolar fumarate hydratase inhibitor (FHIN-1) for 24 h. Three biological replicates per condition. </p> <p>2. TruSeq mRNA stranded analysis of murine Fh1<sup>fl/fl</sup> kidney epithelial cells treated with vehicle control (DMSO) or Atpenin A5 (AA5) for 24 h. Three biological replicates per condition. </p> <p>3. Label-free proteomic analysis of a murine Fh1<sup>fl/fl</sup> kidney epithelial cells treated with vehicle control (DMSO) or 20 micromolar FHIN-1 for 24 h. Five biological replicates per condition. </p> <p>4. Label-free proteomic analysis of a murine Fh1<sup>fl/fl</sup> kidney epithelial cells treated with vehicle control (DMSO) and 500 micromolar thenoyltrifluoroacetone (TTFA). Five biological replicates per condition. </p> <p>5. Western blot uncropped blots</p>
Single-cell TCA cycle proteomics of human embryos during early organogenesis
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Effect of Fatty Liver on TCA Cycle Flux and the Pentose Phosphate Pathway
ClinicalTrials.gov study NCT03480594. IPD Sharing: NO. Countries: 1. Publications: 21.
Disruption of the TCA cycle reveals an ATF4-mediated integration of redox and amino acid metabolism
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The TCA (TriCarboxylic Acid) Cycle/Citric Acid Cycle/Krebs cycle acts as a central metabolic hub
<p>Diagram depicting the TCA (TriCarboxylic Acid) Cycle/Citric Acid Cycle/Krebs cycle and its relation to a variety of macromolecules</p>
MITF controls the TCA cycle to modulate the melanoma hypoxia response
GEO Series GSE132624. Homo sapiens. 36 samples. Type: Expression profiling by high throughput sequencing.
Gene expression analysis of a double knockout of TCA cycle enzymes IDH and KDH
GEO Series GSE59015. Plasmodium falciparum. 16 samples. Type: Expression profiling by array.
Blocking the TCA Cycle in Cancer Cells Potentiates CD36+ T-cell-Mediated Antitumor Immunity by Suppressing ER Stress-Associated THBS2 Signaling
GEO Series GSE243460. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
O-GlcNAcylation regulates c-Myc/PDK2 axis to rewire TCA cycle and promote colorectal tumor growth
GEO Series GSE232258. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
TCA Cycle in the Dentate in Friedreich's Ataxia
ClinicalTrials.gov study NCT03122925. IPD Sharing: NO. Countries: 1. Publications: 0.
ABHD11 mediated deglutarylation regulates the TCA cycle and T cell metabolism
GEO Series GSE292544. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.