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7 results for “Tamaricaceae”
Dataset: DNA barcodes and microsatellites: how they complement for species identification in the complex genus Tamarix (Tamaricaceae)
<p class="CuerpoA">DNA barcoding allows the identification of an organism by comparing the sequence of selected DNA regions (barcodes) with a previously compiled database, and it can be useful for taxonomic identification of species in complex genera, such as <i>Tamarix</i>. Many species of this genus show convergent morphology, which leads to frequent errors in their identification. Highly variable genetic markers, such as microsatellites or short sequence repeats (SSR), could be used to differentiate species where DNA barcodes fail. Here, we tested the ability of both, five different marker regions (<i>rbcL</i>, <i>matK</i>, ITS, <i>trnH</i>-<i>psbA</i>, and <i>ycf1</i>), and 14 microsatellites, to properly identify <i>Tamarix</i> species, especially those from the Mediterranean Basin, and compared the pros and cons of the different analytical methods for species identification. DNA barcoding allows the genetic identification of certain species in <i>Tamarix</i>. The two-locus barcodes <i>matK</i>+ITS and ITS+<i>ycf1</i> were the best-performing combinations, allowing up to 69% and 70%, respectively, correct identification. However, DNA barcoding failed in phylogenetically close groups, such as many Mediterranean species. The use of SSR can aid the identification of species, and the combination of both types of data (DNA barcoding and SSR) improved the success. The combination of data was especially relevant in detecting the presence of hybridization processes, which are common in the genus. However, caution must be exercised when choosing the clustering methods for the SSR data, since different methods can lead to very different results.</p>
Dataset: DNA barcodes and microsatellites: how they complement for species identification in the complex genus Tamarix (Tamaricaceae)
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FIGURE 3 in Spatiotemporal evolution of Reaumuria (Tamaricaceae) in Central Asia: insights from molecular biogeography
FIGURE 3. Reconstructions of ancestral areas performed with S-DIVA on the left, Lagrange on the right, for several nodes, and the most similar states (areas) with largest frequencies calculated by RASP, are illustrated on the right. Pie charts at the internal nodes represent the calculated probabilities (relative frequencies) of alternative ancestral area reconstructions. In the right figure, several dispersals are indicated by arrows on the branches. Node numbers (1~9) are in italics at the right of the node, the same as in Fig. 2. Area letters as stated in the text: A: Tianshan Mountains; B: Pamir-Alai mountains; C: eastern Central Asia; D: western Central Asia; E: Iran-Turkey; and F: Mediterranean. A detailed description is in the text.
FIGURE 2 in Spatiotemporal evolution of Reaumuria (Tamaricaceae) in Central Asia: insights from molecular biogeography
FIGURE 2. Phylogenetic tree and chronogram using BEAST Bayesian inference. Values at left of nodes on the tree are bootstrap support above, and posterior probability below. At the right of nodes are the estimated dating values and their 95% HPD from BEAST. Node numbers (1~9) are in italic at the right of nodes. The classification system derived from tree construction, including two sections and five series, is shown on the right of figure, and detailed in the Appendix.
FIGURE 1 in Spatiotemporal evolution of Reaumuria (Tamaricaceae) in Central Asia: insights from molecular biogeography
FIGURE 1. Distribution of Reaumuria, modified from Hao et al. (2013). Three species of series Kaschgaricae are detailed in Fig. 1c, six distribution areas, and westward and eastward idspersals conducted from biogeographical history reconstruction are illustrated in Fig. 1d.
FIGURE 4. For S in Spatiotemporal evolution of Reaumuria (Tamaricaceae) in Central Asia: insights from molecular biogeography
FIGURE 4. For S-DIVA results, the calculations of its biogeographical events including dispersal, vicariance and extinction, produced in RASP, are shown.
Figs. 1–2. Splendid tamarisk weevil, Coniatus splendidulus. 1 in First Record of Coniatus splendidulus (Fabricius) (Coleoptera: Curculionidae) in Baja California, Mexico, Collected from Tamarixhohenackeri Bunge (Tamaricaceae)
Figs. 1–2. Splendid tamarisk weevil, Coniatus splendidulus. 1) Habitus, lateral view; 2) Adult perched on a branch of Tamarix hohenackeri.
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