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73 results for “Target families”
Human Family With Sequence Similarity 83 Member B (FAM83B); A Target Enabling Package
<p>FAM83A-H are newly identified oncogenes characterised by a conserved DUF1669 domain. FAM83B can substitute for RAS to promote malignant transformation. Ablation of FAM83B or mutation of Lys230 inhibits malignant phenotypes, implicating FAM83B as potential therapeutic target. As part of this TEP, we solved the first crystal structures from the FAM83 family, including FAM83A and FAM83B. The structures of the DUF1669 domain reveal a phospholipase D-like fold lacking conservation of key catalytic residues. We deorphanise the FAM83 DUF1669 domain as a critical docking scaffold for binding of casein kinase 1 isoforms. Finally, using XChem fragment screening we report chemical fragments that bind to Lys230 in the central pocket of the DUF1669 and form starting points for potential drug development.</p>
Human Poly (ADP-ribose) Polymerase Family Member 14 (PARP14); A Target Enabling Package
<p>This work provides reagents to develop specific inhibitors of the macrodomains of PARP14, for potential use for cancer and/or inflammation. Targeting the macrodomains of PARP14 is an alternative targeting strategy to PARP catalytic domain inhibitors that may allow greater inhibitor selectivity, and an alternative cellular effect.</p> <p>This package includes protein purification protocols, crystal structures of the 2nd and 3rd macrodomain of PARP14 in complex with small molecule chemical starting points, <em>in vitro </em>assays to measure ligand binding to the macrodomains, as well as validation of a PARP14 antibody and reagents to generate PARP14 knock-out cell lines (CRISPR-Cas9).</p>
A target capture approach for phylogenomic analyses at multiple evolutionary timescales in rosewoods (Dalbergia spp.) and the legume family (Fabaceae)
<p>Understanding the genetic changes associated with the evolution of biological diversity is of fundamental interest to molecular ecologists. The assessment of genetic variation at hundreds or thousands of unlinked genetic loci forms a sound basis to address questions ranging from micro- to macro-evolutionary timescales, and is now possible thanks to advances in sequencing technology. Major difficulties are associated with i) the lack of genomic resources for many taxa, especially from tropical biodiversity hotspots, ii) scaling the numbers of individuals analyzed and loci sequenced, and iii) building tools for reproducible bioinformatic analyses of such datasets. To address these challenges, we developed a set of target capture probes for phylogenomic studies of the highly diverse, pantropically distributed and economically significant rosewoods (<em>Dalbergia</em> spp.), explored the performance of an overlapping probe set for target capture across the legume family (Fabaceae), and built a general-purpose bioinformatics pipeline. Phylogenomic analyses of <em>Dalbergia</em> species from Madagascar yielded highly resolved and well supported hypotheses of evolutionary relationships. Population genomic analyses identified differences between closely related species and revealed the existence of a potentially new species, suggesting that the diversity of Malagasy <em>Dalbergia</em> species has been underestimated. Analyses at the family level corroborated previous findings by the recovery of monophyletic subfamilies and many well-known clades, as well as high levels of gene tree discordance, especially near the root of the family. The new genomic and bioinformatics resources will hopefully advance systematics and ecological genetics research in legumes, and promote conservation of the highly diverse and endangered <em>Dalbergia</em> rosewoods.</p>
Fig. 4 in Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa
Fig. 4. Distribution of specimen ages and the number of loci recovered in the phylogenomic study of Ochnaceae. A, Histogram of the collection years of all Ochnaceae specimens; B & C, Relationship between the year of collection of the specimens and the number of loci recovered for tissue obtained from herbarium material (excluding specimens with silica-dried leaf material), analysed for Ochneae and all the remaining Ochnaceae separately, either using the consensus-alignment (B) or the sample-specific (C) reference-based assembly approach. Pearson correlation coefficients and confidence intervals are given for each group.
Fig. 2 in Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa
Fig. 2. RAxML trees based on the concatenated nuclear loci of Ochnaceae. A, Early-diverging branches of Ochnaceae and relationships within Quiinoideae based on the FAM dataset; B, Phylogenetic relationships of Sauvagesieae, Luxemburgieae and Testuleeae based on the SLT dataset. Numbers on the branches are bootstrap values>50%. Numbers in parentheses after species names correspond to the specimen IDs (only for species with multiple accessions). The indicated classification of subfamilies and tribes follows Schneider & al. (2014).
Fig. 1 in Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa
Fig. 1. Overview of the phylogenetic relationships of the major clades of Ochnaceae based on the FAM dataset together with images of representative species. The classification follows Schneider & al. (2014). Ochninae is by far the most species-rich clade comprising about two-thirds of the family's species and six genera (Brack. = Brackenridgea; Cmp. = Campylospermum, clades A and B; I. = Idertia; Ochna; Ouratea; Rh. = Rhabdophyllum). Letters around the tree refer to the photos (mostly flowers except where indicated) and the relative position of the displayed taxa on the tree. A, Medusagyne oppositifolia (Medusagynoideae); B, Froesia venezuelensis (Quiinoideae); C, Luxemburgia schwackeana (Luxemburgieae); D, Rhytidanthera sulcata; E, Cespedesia spathulata; F, Poecilandra retusa; G, Godoya antioquiensis; H, Wallacea insignis; I, Sauvagesia semicylindrifolia; J, Sauvagesia erecta (Sauvagesieae); K, Infructescence of Lophira lanceolata with accrescent sepals (Lophirinae); L, Flower of Elvasia kollmannii (Elvasiinae); M, Perissocarpa umbellifera; N, Fruiting Rhabdophyllum arnoldianum; O, Brackenridgea nitida; P, Campylospermum glaberrimum; Q, Ochna serrulata; R, Fruit of Ochna integerrima with drupelets sitting on enlarged receptacle; S, Fruit of Ouratea sp. with enlarged red receptable; T, Ouratea sp. — Photos: A, K & N from www.africanplants.senckenberg.de (Dressler & al., 2014–); B by Julio Schneider; C by William Milliken/ Royal Botanic Gardens, Kew; D by Sandra Reinales; E by Reinaldo Aguilar; F, H & M by Francisco Farroñay; G by John Clark; I, J, S & T by Domingos Cardoso; L by Claudio Nicoletti de Fraga; O by John Elliott; P by Warran McCleland; Q by Marja Broersma; R by Pierre Grard.
A target capture approach for phylogenomic analyses at multiple evolutionary timescales in rosewoods (Dalbergia spp.) and the legume family (Fabaceae)
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Data from: Targeted enrichment of large gene families for phylogenetic inference: phylogeny and molecular evolution of photosynthesis genes in the Portullugo clade (Caryophyllales)
Hybrid enrichment is an increasingly popular approach for obtaining hundreds of loci for phylogenetic analysis across many taxa quickly and cheaply. The genes targeted for sequencing are typically single-copy loci, which facilitate a more straightforward sequence assembly and homology assignment process. However, this approach limits the inclusion of most genes of functional interest, which often belong to multi-gene families. Here we demonstrate the feasibility of including large gene families in hybrid enrichment protocols for phylogeny reconstruction and subsequent analyses of molecular evolution, using a new set of bait sequences designed for the "portullugo" (Caryophyllales), a moderately sized lineage of flowering plants (∼2200 species) that includes the cacti and harbors many evolutionary transitions to C4 and CAM photosynthesis. Including multi-gene families allowed us to simultaneously infer a robust phylogeny and construct a dense sampling of sequences for a major enzyme of C4 and CAM photosynthesis, which revealed the accumulation of adaptive amino acid substitutions associated with C4 and CAM origins in particular paralogs. Our final set of matrices for phylogenetic analyses included 75–218 loci across 74 taxa, with ∼50% matrix completeness across datasets. Phylogenetic resolution was greatly improved across the tree, at both shallow and deep levels. Concatenation and coalescent-based approaches both resolve the sister lineage of the cacti with strong support: Anacampserotaceae + Portulacaceae, two lineages of mostly diminutive succulent herbs of warm, arid regions. In spite of this congruence, BUCKy concordance analyses demonstrated strong and conflicting signals across gene trees. Our results add to the growing number of examples illustrating the complexity of phylogenetic signals in genomic-scale data.
A target enrichment probe set for resolving phylogenetic relationships in the coffee family, Rubiaceae
<p><em>Rubiaceae </em>is among the most species-rich, morphologically and geographically diverse plant families. Phylogenies have been inferred for many different groups across the family, however these have mostly relied on few genomic and plastid loci, as opposed to large-scale genomic data. Target enrichment provides the ability to generate sequence data for hundreds to thousands of phylogenetically informative, single-copy loci, which often leads to improved phylogenetic resolution at both shallow and deep taxonomic scales; however, a publicly accessible <em>Rubiaceae</em>-specific probe set that allows for comparable phylogenetic inference across clades is lacking. Here, we use publicly accessible genomic resources to identify putatively single copy nuclear loci for target enrichment in two <em>Rubiaceae </em>tribes: Hillieae (<em>Cinchonoideae</em>) and Palicoureeae+Psychotrieae (<em>Rubioideae</em>). We sequenced 2270 exons corresponding to 1059 supercontigs in our target clades, and generated in silico target enrichment sequences for other <em>Rubiaceae </em>taxa using our designed probe set. Our probe set, which we call <em>Rubiaceae </em>2270, was effective for targeting loci in species across and even outside of <em>Rubiaceae</em>. This probe set will facilitate phylogenomic studies in <em>Rubiaceae </em>and advance systematics and macroevolutionary studies in the family.</p>
ER values of the targeted by-products as a function of their metallogenic family. The bold text highlights the most relevant metal association regarding the selected metallogenic families.
<p>This dataset is table 3 from the article "Predictive assessment of metallogenic signatures using the DataBase Querying (DBQ) method: A European application by Blandine Gourcerol *, Guillaume Bertrand, Laurent Bailly, Pauline Moreau, Isabelle Duhamel-Achin, Maxime Picault, Philippe Négrel</p> <p>DOI : https://doi.org/10.1016/j.gexplo.2022.106966</p>
Data for a preliminary molecular phylogeny of the family Hydroptilidae (Trichoptera): exploring the combination of targeted enrichment data and legacy Sanger sequence data
<p><span>The purpose of this study is to provide a proof-of-concept that the use of molecular data, particularly targeted enrichment data, and statistically supported methods of analysis can result in the construction of a stable phylogenetic framework for the microcaddisflies (Trichoptera: Hydroptilidae). Here, we use a combination of targeted enrichment data for ca. 300 nuclear protein-coding genes and legacy (Sanger-based) sequence data for the mitochondrial COI gene and partial sequence from the 28S rRNA gene.</span></p>
Target capture data resolve recalcitrant relationships in the coffee family (Rubioideae, Rubiaceae)
<p class="MsoNormal"><span>Subfamily Rubioideae is the largest of the main lineages in the coffee family (Rubiaceae), with over 8,000 species and 29 tribes. Phylogenetic relationships among tribes and other major clades within this group of plants are still only partly resolved despite considerable efforts. While previous studies have mainly utilized data from the organellar genomes and nuclear ribosomal DNA, we here use a large number of low-copy nuclear genes obtained via a target capture approach to infer phylogenetic relationships within Rubioideae. We included 101 Rubioideae species representing all but two (the monogeneric tribes Foonchewieae and Aitchinsonieae) of the currently recognized tribes, and all but one non-monogeneric tribe were represented by more than one genus. Using data from the 353 genes targeted with the universal Angiosperms353 probe set we investigated the impact of data type, analytical approach, and potential paralogs on phylogenetic reconstruction. We inferred a robust phylogenetic hypothesis of Rubioideae with the vast majority (or all) nodes being highly supported across all analyses and datasets and few incongruences between the inferred topologies. The results were similar to those of previous studies but novel relationships were also identified. We found that supercontigs (coding sequence [CDS] + noncoding sequence) clearly outperformed CDS data in levels of support and gene tree congruence. The full datasets (353 genes) outperformed the datasets with potential paralogous genes removed (186 genes) in levels of support but increased gene tree incongruence slightly. The pattern of gene tree conflict at short internal branches was often consistent with high levels of incomplete lineage sorting (ILS) due to rapid speciation in the group. While concatenation- and coalescence-based trees mainly agreed, the observed phylogenetic discordance between the two approaches may be best explained by their differences in accounting for ILS. The use of target capture data greatly improved our confidence and understanding of the Rubioideae phylogeny, highlighted by the increased support for previously uncertain relationships and the increased possibility to explore sources of underlying phylogenetic discordance.</span></p>
Fig. 3 in Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa
Fig. 3. Continues. For caption, see next part.
Data for a preliminary molecular phylogeny of the family Hydroptilidae (Trichoptera): exploring the combination of targeted enrichment data and legacy Sanger sequence data
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Data from: Targeted enrichment of large gene families for phylogenetic inference: phylogeny and molecular evolution of photosynthesis genes in the Portullugo clade (Caryophyllales)
Open the record for dataset details and reuse information.
A target enrichment probe set for resolving phylogenetic relationships in the coffee family, Rubiaceae
Open the record for dataset details and reuse information.
Target capture data resolve recalcitrant relationships in the coffee family (Rubioideae, Rubiaceae)
Open the record for dataset details and reuse information.
Impact of targetted health education and sensitization of family caregivers upon treatment maintenance and satisfaction among completely edentulous patients suffering from elder abuse and neglect
<p>the following is the raw and coded data associated with the research</p>
Appendix. The status of global taxonomic checklist preparation for flowering plant families (based on Angiosperm Phylogeny Group II but modified to reflect circumscriptions of existing checklists). If a checklist is complete and available on the Internet then the URL is also given. The species numbers (sp. no.) given are either based on actual working lists (WL) where they exist or are based on Stevens (2006) if no WL is available. Five categories are used to describe the status of a particular working list: 1, checklist complete and accessible via the Internet now; 2, checklist available on Internet by end of 2007 (Asteraceae 2010); 3, checklist complete but not online; 4, some online lists giving partial coverage may be available; 5, no global checklist being compiled so far as known. in Towards Target 1 of the Global Strategy for Plant Conservation: A working list of all known plant species - Progress and prospects
Appendix. The status of global taxonomic checklist preparation for flowering plant families (based on Angiosperm Phylogeny Group II but modified to reflect circumscriptions of existing checklists). If a checklist is complete and available on the Internet then the URL is also given. The species numbers (sp. no.) given are either based on actual working lists (WL) where they exist or are based on Stevens (2006) if no WL is available. Five categories are used to describe the status of a particular working list: 1, checklist complete and accessible via the Internet now; 2, checklist available on Internet by end of 2007 (Asteraceae 2010); 3, checklist complete but not online; 4, some online lists giving partial coverage may be available; 5, no global checklist being compiled so far as known.
Fig. 2 in Museomics: Phylogenomics of the Moth Family Epicopeiidae (Lepidoptera) Using Target Enrichment
Fig. 2. Number of raw loci recovered for each sample per year of collection. The dashed line is for reference and represents the trend. Plot made on R.
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Allen Brain Atlas
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.