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Water Quality Data (Grab Samples) from the Taylor Slough, just outside Everglades National Park (FCE), for August 1998 to November 2006
Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these montly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with a YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 1L sample every 15 minutes after the threshold has been reached and remains (previous to 2003- the autosampler was programmed to collect 500mL of water every 30 minutes while the threshold was being met). Rain event samples are collected, retrieved, and analyzed like out c
Soil Physical Data from the Taylor Slough, just outside Everglades National Park (FCE), from October 1998 to October 2006
Soil pH, Eh and temperature readings are taken at TS/Ph4 and TS/Ph5. These measurements are taken only when the marsh is wet. Measurements are taken using an Orion model 250A meter, and the probes attached to the meter are the Orion Thermo pH probe and the Orion Eh probe. All readings are recorded in field notebooks, and then transferred into Microsoft Excel. The Eh reading is taken when the Eh probe is attached to the meter and the word "Ready" appears on the meter screen. The pH and temperature reading are taken when the pH probe is attached to the meter and the word "Ready" appears on the meter screen.
Soil Physical Data from the Taylor Slough, within Everglades National Park (FCE), from September 1999 to November 2006
Soil pH, Eh and temperature readings are taken at TS/Ph1b,TS/Ph2,TS/Ph3 and TS/Ph6b. These measurements are taken only when the marsh is wet. Measurements are taken using an Orion model 250A meter, and the probes attached to the meter are the Orion Thermo pH probe and the Orion Eh probe. All readings are recorded in field notebooks, and then transferred into Microsoft Excel. The Eh reading is taken when the Eh probe is attached to the meter and the word "Ready" appears on the meter screen. The pH and temperature reading are taken when the pH probe is attached to the meter and the word "Ready" appears on the meter screen.
Soil Characteristic and Nutrient Data from the Taylor Slough, within Everglades National Park (FCE), from March 2002 to April 2004
Three random soil cores are collected once a year from TS/Ph 1b, 2, 3, 4, 5, & 6b. The collection of these soil cores is in the dry season (Dec.-May). The top 10cm of soil is collected using a soil core. The soil is then analyzed to obtain its bulk density, organic matter content, TC, TN, and TP.
Monthly fluorescence parallel factor analysis (PARAFAC) components for Shark River Slough, Taylor Slough, and Florida Bay, Everglades National Park (FCE LTER), Florida, USA, April 2011 - ongoing
Dissolved organic matter plays an important role in biogeochemical processes in aquatic environments such as elemental cycling, microbial loop energetics, and the transport of materials across landscapes. Since most of N (> 90%) and P (around 90%) is in the organic form in the oligotrophic subtropical Florida Coastal Everglades (FCE), study of the source and dynamics of dissolved organic matter (DOM) in the ecosystem is crucial for the better understanding of the biogeochemical cycling of nutrients. FCE are composed of estuaries with distinct regions with different biogeochemical processes. Freshwater marsh primarily receives terrestrial input and local autochthonous vegetation production. Mangrove ecotone, nevertheless, is affected by the tidal contributions from Florida Bay and local mangrove production. Florida Bay (FB) is a wedge-shaped shallow oligotrophic estuary which lays south of the Everglades, the bottom of which is covered with a dense biomass of seagrass. The sources of both freshwater and nutrients in FCE are difficult to quantify, owing to the non-point source nature of runoff from the Everglades and the dendritic cross channels in the mangroves. Furthermore, the combination of multiple DOM sources (freshwater marsh vegetation, mangroves, phytoplankton, seagrass, etc.), and the potential seasonal variability of their relative contribution, along with the history of (photo)chemical and microbial diagenetic processing, and complex advective circulation, makes the study of DOM dynamics in FCE particularly difficult using standard schemes of estuarine ecology. Quantitative information of DOM is very useful to investigate the biogeochemical cycling of DOM to a certain degree, however, qualitative information is necessary to better understand the source and dynamics of DOM. Since fluorescence spectroscopic techniques are very sensitive, quick and simple, they have been applied to investigate the fate of DOM in estuaries. Here, we have quantified a series of
Microbial Sampling from Shark River Slough and Taylor Slough, Everglades National Park, South Florida, USA (FCE LTER), January 2001 - December 2023
We collected monthly observations of water microbial characteristics from each of the FCE LTER sites beginning in February 2001. Bacteria counts using DAPI epifluorescence, heterotrophic bacteria production with tritiated Thymidine uptake, and algal energetics and chl a using the PAM fluorometer were determined from each of the water samples. Bacteria counts in Shark River Slough in the freshwater end of the transect revealed highest bacteria numbers upon marsh rewetting after the dry down. The highest overall count of bacteria was found at the most freshwater point adjacent to the perimeter of the water conservation area but only in March of 2001. Peak numbers of bacteria in the saline end of Shark River transect occurred in December and July. There is some evidence that these higher bacteria numbers in the SR transect are associated with higher salinities. Highest bacteria counts along the Taylor slough transect were observed at the mouth of the Taylor River with peak counts in the wet season in October. Bacteria densities also increased from the eastern to western end of the transect in Florida Bay. Heterotrophic bacterial production did not relate closely bacteria numbers and exhibited highest numbers in the dry season. There was no discernible relationship between salinity and bacteria production as seen in bacteria numbers. Monthly observations using PAM fluorometry revealed that brown algae was the greatest contribution to the algal chal a pool and this relationship was consistent across all FCE LTER sites. Converse to the pattern of bacteria abundance along the Shark River transect, there was evidence that algal biomass decreases from freshwater to estuary. In the Shark River, algal energetics increased from marsh to estuary as well as in the panhandle region of the Taylor River transect. From these observations we conclude the behavior of the microbial loop and interactions generated between functional guilds is highly variable along individual transects an
Water Quality Data (Extensive) from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, April 1996 - ongoing
Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these monthly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with an YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.
Water Quality Data (Grab Samples) from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, May 2001 - ongoing
Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these montly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with a YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.
Water Quality Data (Extensive) from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, July 1999 - ongoing
Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these monthly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with an YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.
Water Quality Data (Grab Samples) from the Taylor Slough, Everglades National Park (FCE), Florida, USA, September 1999 - ongoing
Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these montly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with a YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.
Sawgrass Above and Below Ground Total Phosphorus from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, March 2002 - ongoing
Once a year during the dry season (Dec-May) three live sawgrass plants are collected from each site. These plants are divided into their live above and live below ground parts. Then both the above and below ground parts are analyzed for TN, TC, and TP.
Sawgrass Above and Below Ground Total Nitrogen and Total Carbon from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, March 2002 - ongoing
Once a year during the dry season (Dec-May) three live sawgrass plants are collected from each site. These plants are divided into their live above and live below ground parts. Then both the above and below ground parts are analyzed for TN, TC, and TP.
Sawgrass above ground biomass from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, August 1999 - ongoing
Sawgrass biomass is measured for TS/Ph-1, 2, 3, & 6 since 1999; TS/Ph-4 & 5 since 1997 to 2006; SRS-1b, 2, & 3 since 2000; SRS1c for 2005 to 2006, and SRS1d since 2006. Three 1 m2 plots are placed on each site. The sites are measured every two months. The number of sawgrass plants is counted for each 1 m2 plot and one third or a minimum of fifteen plants is measured. For each measured plant, measurements of the total number of live leaves, length of the live leaves, and culm diameter at the base are taken. From these measurements we calculate the average leaf length, and the sum of the length of the leaves. We created a model for biomass using a stepwise regression to see which of the variables measured and calculated are more correlated to explain plant biomass. Obtaining the mean biomass for the plants within the plot and multiplying it by the number of plants counted in the plot calculates biomass for each plot. The total biomass for each plot is summed with the other two plots in the same site, and they are averaged to obtain one biomass number per site. To validate our model, plant clippings are obtained in which four plants are clipped (small, medium, large, inflorescence) from each site. The same measurements are applied to the clipped plants as those applied to the measured plants in the plots. The plant clippings are oven dried at 70 degrees C and weighed.
Periphyton Net Primary Productivity and Respiration Rates from the Taylor Slough, just outside Everglades National Park (FCE), South Florida from December 1998 to December 2004
Periphyton metabolism is being measured at TS/Ph1b, TS/Ph2, TS/Ph3, TS/Ph4, and TS/Ph5 every 6-8 weeks during the wet season. We quantify metabolic rates of periphyton assemblages using standard oxygen change techniques in 300mL light and dark BOD bottles in triplicate. Using YSI dissolved oxygen probes and meters, we measure dissolved oxygen change as the difference of initial and final oxygen concentrations during a two hour incubation period. We calculate net primary productivity and respiration rates in units of oxygen and carbon, then normalize to the organic content (ash free dry weight) of the incubated periphyton.
Precipitation from the Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, July 2000 - ongoing
Rain level data are collected using ISCO rain level gages attached to ISCO autosamplers, which also act as dataloggers. We condense these data into daily cumulative rainfall, although hourly rain level data are also available.
Water Depths and Water Temperatures near Soil Surface from Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, August 1999 - ongoing
Water depth (from August 1999 to present) and water temperature (from May 2021 to present) are recorded hourly at TS/Ph1a, TS/Ph2 and TS/Ph3 and every 30 minutes at TS/Ph6a and TS/Ph7a. Water depth is measured with pressure water level loggers (Infinities USA or HOBO) that record water height relative to the local soil surface. Water temperature near soil surface is measured with HOBO loggers. Note by IM (2021): The water meters at some of the TS sites have been moved over the years as boardwalks have been reconstructed. There is no set survey datum for these sites, so it is impossible to correct the data to an actual datum. For hydrologic applications, it may be better to use water level data from USGS stations.
Quantitative and qualitative aspects of dissolved organic carbon leached from plant biomass in Taylor Slough, Shark River and Florida Bay (FCE) for samples collected in July 2004
Plant biomass was collected from Taylor Slough, Shark River and Florida Bay in Everglades National Park. Samples were taken to the lab and incubated with Milli-Q water in the dark for a period of 36 days. NaN3 was added to half the bottles to test the role of microbial activity on the leaching rates and composition of leachate. Every three days the water was decanted and replaced with fresh Milli-Q water. The decanted samples were filtered and analyzed for DOC concentration, sugar content, and total phenol content.
Physical and microbial processing of dissolved organic nitrogen (DON) (Salinity Experiment) along an oligotrophic marsh/mangrove/estuary ecotone (Taylor Slough and Florida Bay) for August 2003 in Everglades National Park (FCE), South Florida, USA
A better understanding of the biogeochemical cycling of nutrients entering Florida Bay is a key issue regarding the restoration of the Everglades. In addition to precipitation, the other major source of freshwater to Florida Bay is from Taylor Slough and the C-111 Basin in the northeast section of the Bay. While it is known that these areas deliver significant amounts of N to the Bay, a significant portion of this is in the form of dissolved organic N (DON). The sources, environmental fate and bioavailability to microorganisms of this DON are however, not known. Should this DON be readily available, any increased load as a function of restoration changes might have an impact on internal phytoplankton bloom dynamics. No significant flocculation or precipitation of DOM occurred with increase in salinity, meaning that terrestrial DOM does not get trapped in the sediments but stays in the water column where it subjected to photolysis and advective transport. Sunlight has a significant effect on the chemical characteristics of DOM. While the DOC levels did not change significantly during photo-exposure, the optical characteristics of the DOM were modified. The environmental implications of this are conflicting: photo-induced polymerization may stabilize the DOM by reducing its bioavailability while photolysis may make the DOM more labile. Overall, DON bioavailability was relatively low in this region. Even though the amount of DON loaded to the bay may be significant, the fraction of DON available for microbial cycling is much smaller. The amount of N supplied by recycling may be a significant portion of the total DIN pool. All this must be considered in context with the proposed CERP modifications to flows. As of the latest initial Comprehensive Everglades Restoration Project (CERP) update, the flows to Taylor Slough and C-111/Panhandle Basis are not predicted to change very much from base conditions. Therefore we do not expect any great increases in TN loading in this
Physical and microbial processing of dissolved organic nitrogen (DON) (Photodegradation Experiment) along an oligotrophic marsh/mangrove/estuary ecotone (Taylor Slough and Florida Bay) for August 2003 in Everglades National Park (FCE), South Florida, USA
A better understanding of the biogeochemical cycling of nutrients entering Florida Bay is a key issue regarding the restoration of the Everglades. In addition to precipitation, the other major source of freshwater to Florida Bay is from Taylor Slough and the C-111 Basin in the northeast section of the Bay. While it is known that these areas deliver significant amounts of N to the Bay, a significant portion of this is in the form of dissolved organic N (DON). The sources, environmental fate and bioavailability to microorganisms of this DON are however, not known. Should this DON be readily available, any increased load as a function of restoration changes might have an impact on internal phytoplankton bloom dynamics. No significant flocculation or precipitation of DOM occurred with increase in salinity, meaning that terrestrial DOM does not get trapped in the sediments but stays in the water column where it subjected to photolysis and advective transport. Sunlight has a significant effect on the chemical characteristics of DOM. While the DOC levels did not change significantly during photo-exposure, the optical characteristics of the DOM were modified. The environmental implications of this are conflicting: photo-induced polymerization may stabilize the DOM by reducing its bioavailability while photolysis may make the DOM more labile. Overall, DON bioavailability was relatively low in this region. Even though the amount of DON loaded to the bay may be significant, the fraction of DON available for microbial cycling is much smaller. The amount of N supplied by recycling may be a significant portion of the total DIN pool. All this must be considered in context with the proposed CERP modifications to flows. As of the latest initial Comprehensive Everglades Restoration Project (CERP) update, the flows to Taylor Slough and C-111/Panhandle Basis are not predicted to change very much from base conditions. Therefore we do not expect any great increases in TN loading in this
Characterization of dissloved organic nitrogen in an oligotrophic subtropical coastal ecosystem (Taylor Slough and Shark River Slough) for December 2001 in Everglades National Park (FCE), South Florida, USA
A better understanding of the biogeochemical cycling of nutrients entering Florida Bay is a key issue regarding the restoration of the Everglades. In addition to precipitation, the other major source of freshwater to Florida Bay is from Taylor Slough and the C-111 Basin in the northeast section of the Bay. While it is known that these areas deliver significant amounts of N to the Bay, a significant portion of this is in the form of dissolved organic N (DON). The sources, environmental fate and bioavailability to microorganisms of this DON are however, not known. Should this DON be readily available, any increased load as a function of restoration changes might have an impact on internal phytoplankton bloom dynamics. No significant flocculation or precipitation of DOM occurred with increase in salinity, meaning that terrestrial DOM does not get trapped in the sediments but stays in the water column where it subjected to photolysis and advective transport. Sunlight has a significant effect on the chemical characteristics of DOM. While the DOC levels did not change significantly during photo-exposure, the optical characteristics of the DOM were modified. The environmental implications of this are conflicting: photo-induced polymerization may stabilize the DOM by reducing its bioavailability while photolysis may make the DOM more labile. Overall, DON bioavailability was relatively low in this region. Even though the amount of DON loaded to the bay may be significant, the fraction of DON available for microbial cycling is much smaller. The amount of N supplied by recycling may be a significant portion of the total DIN pool. All this must be considered in context with the proposed CERP modifications to flows. As of the latest initial Comprehensive Everglades Restoration Project (CERP) update, the flows to Taylor Slough and C-111/Panhandle Basis are not predicted to change very much from base conditions. Therefore we do not expect any great increases in TN loading in this
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