Find research datasets worth reusing

opencc-by-4.0Apr 2018View details →

Fig. 1 in The identification of Theileria bicornis in captive rhinoceros in Australia

Fig. 1. Photomicrograph of Diff-Quik stained blood smear from black rhinoceros, Siabuwa infected with T. bicornis. The morphology of T. bicornis is not well-described in the literature. We observed comma-shaped piroplasms approximately 1.5 μm in length closely resembling T. orientalis. Ring forms were also occasionally observed (not shown). Bar = 5 μm.

opencc-by-4.0Apr 2018View details →

Fig. 2 in The identification of Theileria bicornis in captive rhinoceros in Australia

Fig. 2. Molecular phylogenetic analysis of the piroplasm 18S rRNA gene, including the three rhinoceros samples (Aluka, Umfana and Siabuwa) used in this study. The 18S rRNA sequences were extracted from Genbank during BLAST analysis. The rooted phylogenetic tree was constructed using the neighbour-joining method with T. gondii forming the outgroup. Bootstrap percentages are represented on each node based on 1000 replicates. Phylogenetic analyses were conducted using the PHYLIP packages (Felsenstein, 2005). The haplotypes of the three T. bicornis sequences from this study are indicated at the end of the sequence label.

opencc-by-4.0Apr 2018View details →

Fig. 3 in The identification of Theileria bicornis in captive rhinoceros in Australia

Fig. 3. Truncated alignment of the 18S rRNA T. bicornis haplotypes including the new haplotype H4 identified in this study. Geneious version (7.1.9) (Kearse et al., 2012) was used to generate alignments to highlight the differences between the four T. bicornis haplotypes. 18S rRNA sequences of the three previously described T. bicornis haplotypes H1 to H3 (accession numbers KC771140 to KC771142 respectively) were extracted from Genbank for this analysis. The new T. bicornis haplotype H4 was submitted to Genbank and assigned accession number MF567493.

opencc-by-4.0Jun 2020View details →

Fig. 1 in The Rhipicephalus appendiculatus tick vector of Theileria parva is absent from cape buffalo (Syncerus caffer) populations and associated ecosystems in northern Uganda

Fig. 1 Map showing the sampling sites. The three national parks are indicated with red dots and the cattle sampling sites adjacent to the parks depicted as green dots

Fig. 2 Infected R in Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system

Fig. 2 Infected R. appendiculatus adult ticks feeding on a silicone membrane. a A representation of adult ticks attached to a silicone membrane. b Detection of tick salivary gland infected with T. parva via p104 PCR. Amplicons were visualized in 2% agarose gel

Fig. 5 in Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system

Fig. 5 Determination of the minimum dose of T. parva sporozoites from the in vitro tick feeding system sufficient to infect bovine lymphocytes in vitro. Lymphocytes were probed with anti-PIM monoclonal antibody. a Unstimulated and ConA-stimulated negative controls. b Flow cytometric detection of infected lymphocytes exposed to tenfold serial dilutions of T. parva sporozoites isolated from the in vitro tick feeding system

Fig. 4 in Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system

Fig. 4 Demonstration of infectivity of secreted T. parva sporozoites collected from the in vitro tick feeding system. a Flow cytometric detection of cultured T. parva-infected lymphocytes. b Immunocytochemistry demonstrating T. parva schizont formation in bovine lymphocytes. Lymphocytes were probed with monoclonal antibodies: left panel, isotype control; right panel, anti-PIM. Red indicates antibody-specific reactivity to T. parva within bovine lymphocytes. Scale bar: 20 µm

Fig. 3 in Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system

Fig. 3 Immunohistochemical detection of T. parva salivary gland acinus colonization in adult ticks. Tick sections were probed with monoclonal antibodies: a anti-PIM; b anti-p67; c isotype control. Red indicates antibody-specific reactivity to T. parva colonies. Scale bar: 50 µm

Fig. 1 in Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system

Fig. 1 Infection of R. appendiculatus via acquisition feeding on a T. parva-infected calf. a Calf was infected via subcutaneous inoculation with T. parva salivary gland stabilate. The calf developed severe fever beginning 10 days post-infection. Red arrow indicates nymphal tick application and green arrows indicate collection of replete nymphs. b Detection of T. parva via p104 PCR. Amplicons were visualized in 2% agarose gel

opencc-by-4.0Mar 2015View details →

Fig. 2. Kimura 2 in First record of a spotted fever group Rickettsia sp. and Theileria annulata in Hyalomma dromedarii (Acari: Ixodidae) ticks in the United Arab Emirates

Fig. 2. Kimura 2-parameter distance Neighbor Joining (NJ) tree of 12 nucleotide sequences belonging to the genus Theileria. Codes denote the accession numbers of NCBI GenBank database. * Sequence from Al-Ain, UAE. Numbers next to branches are the percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) (Felsenstein 1985).

Fig. 2. Post mortem examination. Post mortem examination for typical calves showing signs associated with Theileria infection. A in Exposure of vaccinated and naive cattle to natural challenge from buffalo-derived Theileria parva

Fig. 2. Post mortem examination. Post mortem examination for typical calves showing signs associated with Theileria infection. A: Copious frothy exudate from nasal cavities of BJ031 and BJ037. B: Pleural exudate in thoracic cavities of BJ033 and BJ041. C: Frothing in trachea of BJ026 and BJ033.

Fig. 1 in Exposure of vaccinated and naive cattle to natural challenge from buffalo-derived Theileria parva

Fig. 1. Kaplan–Meier survival analysis. Kaplan–Meier survival analysis of vaccinated (dashed line) and control (solid line) calves following field exposure. The black circle and red cross at day 30 represent the animals remaining alive at the end of the experiment (censored). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Fig. 2 in Identification of novel Theileria genotypes from Grant's gazelle

Fig. 2. Phylogenetic analysis of Theileria genotypes isolated from Grant's gazelles (Nanger granti) in Kenya. Bayesian analysis of a 400 nucleotide fragment of the 18S ribosomal RNA gene from 1 sequence of Toxoplasma gondii, 33 Theileria sequences from GenBank, and 3 representative Theileria sequences from Grant's gazelles (GG1, GG2, GG3) in this study (bold font). The tree is rooted on the lineage of T.gondii. Numbers above the branches indicate bootstrap support based on 1000 replicates. Host species, geographic location of isolation, and GenBank accession numbers of the sequences are provided where known. Numbered sequences are listed in Table 1.

Fig. 1 in Identification of novel Theileria genotypes from Grant's gazelle

Fig. 1. Light microscopy of a blood smear stained with Giemsa showing single and paired hemoparasites (highlighted by arrows).

opencc-by-4.0Apr 2015View details →

Fig. 2 in A review of Theileria diagnostics and epidemiology

Fig. 2. Phylogenetic analysis of Theileria genotypes. The 18S ribosomal RNA sequences were extracted from GenBank using text based queries and BLAST analysis. Final analysis and manual curating. The dataset were trimmed to include the V4 hyper-variable region, aligned using MAFFT (auto, 200PAM/k = 2; Katoh and Standley, 2013) and matrix = number of differences) with Mega5 (Tamura et al., 2011). Host and geographic data for genotypes are indicated and were extracted from GenBank files or literature

Fig. 4. Maximum Likelihood phylogenetic tree generated using N in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes

Fig. 4. Maximum Likelihood phylogenetic tree generated using N-terminal sequences of T. sp. (buffalo) and T. parva PIM antigen genes. Maximum composite likelihood trees were constructed using 1000 bootstrap replicates as implemented in MEGA5; the optimal nucleotide substitution model was identified using data monkey. The tree constructed with RAxML (Stamatakis et al., 2014) using a GTR/G/I model with 100 bootstrap iterations.

Fig. 5 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes

Fig. 5. Maximum Likelihood Phylogenetic trees illustrating the genetic relationships of T. parva CD8 T target antigen gene orthologues from T. sp. (buffalo). Panel (A) Tp6; Panel B Tp7: Panel C Tp8. Sequences were aligned and used to construct a maximum likelihood tree, at which the nodes were confirmed using 1000 bootstrap replications. The bootstrap values indicating the degree of support for each node are shown and also the GenBank accession numbers of the sequences. For Tp6, the tree was rooted using the prohibitin gene sequences present in Babesia bovis (XM001609045) and Theileria orientalis (AB161472). For Tp7, the tree was rooted using the putative Heat shock protein 90 gene sequences from Toxoplasma gondii (AY344115), Babesia bovis (AK442026) and Theileria annulata (XM_947380). For Tp8, the tree was rooted using an orthologue of Tp8 found in Theileria equi (CP001669).

Fig. 3 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes

Fig. 3. PCR amplification of genes encoding Theileria parva antigens from Marula schizont-infected leukocyte cultures. Panel A, p104 primers; Panel B PIM, primers; Panel C p67 primers. The order of the schizont-infected lymphocyte samples is (1) N6; (2). N13; (3). N18; (4). N20; (5). N33; (6). N36; (7). N38; (8). N43; (9). N50; (10). N55; (11). N69; (12). N76; (13). N77, (14). N79; (15). N86, (16). N88; (17). N99; (18). N100; (19). N102; (20). N103; (21). N106; (22). N107.

Fig. 2 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes

Fig. 2. Results of a semi-nested PCR assay used to amplify 18S ribosomal subunit DNA using primers specific for T. parva and T. sp. (buffalo). Samples are as follows: 1)N13 2)N18 3) N20 4)N33 5)N36 6) N43 7)N50 8)N55 9) N69 10)N76 11) N79 12) N86 13) N88 14) N99 15)N100 16) N102 17) N103 18)N107 19—21) T. parva clones 22—24) T. sp. (buffalo) clones (documented in Table 2).

Fig. 1 in The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes

Fig. 1. Reverse line blot analysis of schizont cultures containing parasites isolated from Marula farm. The following species-specific oligonucleotide probes were used (a) T. annulata, (b) T. parva, (c) T. mutans, (d) T. velifera, (e) T. taurotragi, (f) T. buffeli, (g) T. sp. (buffalo). (h) B. bigemina, (i) B. bovis. The order of the experimental samples hybridized is DNA from cell culture isolates in lanes 1—22 was lane 1; (1) N6, (2) N13, (3) N18, (4) N20, (5) N33, (6) N36, (7) N38 (8) N43, (9) N50 (10) N55, (11) N69, (12) N76, (13) N77, (14) N79, (15) N88, (16) N99, (17) N100 (18) N103, (19) N106, (20) N107, (21) N86, (22) N102 and DNA extracted from whole cattle blood (23) N106 (24) N69 (25) N86.