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6 results for “Thrips palmi”
Association between susceptibility of Thrips palmi to spinetoram and frequency of G275E mutation provides basis for molecular diagnostics of field-evolved resistance
<p><b><span>Background</span></b><span>: Spinosyn resistance is an increasing problem in field control of targeted pests. While putative mechanisms underlying spinosyn resistance have been identified in controlled studies on many species, mechanisms underlying field-evolved resistance and the development of a molecular diagnostic method for monitoring field resistance have lagged behind. Here, we examined levels of resistance of melon thrips, <i>Thrips palmi,</i> to spinetoram as well as target site mutations in field populations across China to identify potential mechanisms and useful molecular markers for diagnostic purposes.</span></p> <p><b><span>Results</span></b><span>: LC<sub>50</sub> </span><span>of 16 field-collected populations to the spinetoram varied from 0.12 to 759.34 </span>mg L<sup>-1</sup><span>. In resistant populations, we identified the G275E mutation, which has previously been linked to spinosyns resistance, as well as another nonsynonymous mutation, F314V, both located in the <i>α</i>6 subunit of the nicotinic acetylcholine receptor. There was a strong correlation between levels of spinetoram resistance and allele frequency of G275E mutation in field-collected populations (<i>r<sup>2</sup></i> = 0.84) and those reared under laboratory conditions for two to five generations (<i>r<sup>2</sup></i> = 0.91). LC<sub>50</sub> ranged from 0.12 to 0.66 </span>mg L<sup>-1</sup><span> in populations without </span><span>G275E mutation, while it ranged from </span>33.12<span> to 39.91</span> mg L<sup>-1</sup><span> in most populations with a </span><span>G275E mutation frequency > 90%, with the exception of one field-collected population which had an G275E frequency of 92% and a very high LC<sub>50</sub> value of 759.34</span> mg L<sup>-1</sup><span>, suggesting additional mechanisms.</span></p> <p><b><span>Conclusions: </span></b><span>Our results indicate that field-evolved resistance of <i>T. palmi</i> to spinetoram in China is mainly conferred by the G275E mutation, with other mechanisms contributing to a higher level of resistance. The frequency of the G275E mutation provides a useful diagnostic for quantifying resistance levels in field populations of <i>T. palmi</i>.</span></p>
Thrips palmi ̶ Pest Report and Datasheet to support ranking of EU candidate priority pests
<p>These two files are part of the outputs produced under the mandate <a href="http://registerofquestions.efsa.europa.eu/roqFrontend/wicket/page?1-1.ILinkListener-contentPane-listContainer-pageable-21-mandateNumberLnk">M-2017-0056</a> of the European Commission requesting EFSA for technical assistance in the field of quarantine pests qualifying as priority pests as by Article 6(2) of the Regulation (EU) 2016/2031 <em>on protective measures against pests of plants</em>.</p> <p>Under the mandate EFSA produced: i) 1 methodology report (DOI available at the field "Related/alternate identifiers"), ii) 28 datasheets, one for each of the 28 candidate pests, and iii) 28 pest reports supporting the information provided in the datasheets.</p> <p>EFSA wishes to acknowledge the contribution of Antoon Loomans, Alan MacLeod, Lucia Zappalà to the EKE and the review conducted by Nikolaos Papadopoulos.</p>
Data from: Variable resistance to spinetoram in populations of Thrips palmi across a small area unconnected to genetic similarity
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Association between susceptibility of Thrips palmi to spinetoram and frequency of G275E mutation provides basis for molecular diagnostics of field-evolved resistance
Open the record for dataset details and reuse information.
FIGURES 179–193. Thrips species. T. palmi, female 179–183 in Review of the genus <i>Thrips</i> and related genera (Thysanoptera, Thripidae) from Japan
FIGURES 179–193. Thrips species. T. palmi, female 179–183; (179) Head & pronotum; (180) Antenna, right; (181) Metascutum; (182) Tergites VIII–X. T. palmi, male 183–184: (183) Tergites VIII–IX; (184) Sternite VI, sculpture omitted. T. pini, female 185–193: (185) Head & pronotum; (186) Anteromedian area of head; (187) Antenna, right; (188) Metascutum; (189) Tergite VI & pleurotergite, right side; (190) Tergites VIII–X; (191) Sternite VI. T. pini, male 192–193: (192) Tergites VIII–IX; (193) Sternite V, sculpture omitted.
FIGURES 72–83. Thrips species. T. palmi 72–74 in The genus Thrips (Thysanoptera, Thripidae) in Australia, New Caledonia and New Zealand
FIGURES 72–83. Thrips species. T. palmi 72–74: (72) Head & pronotum; (73) Mesometanota; (74) Tergites VI–VIII. T. parvispinus 75–76: (75) Mesometanota; (76) Sternites V–VII. T. phormiicola 77–79: (77) Head & pronotum; (78) Mesometanota; (79) sternites II–III. T. seticollis 80–83: (80) Mesometanota; (81) Pronotum; (82) Tergites VI–VIII; (83) fore tarsus.
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