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19 results for “Trypanosomatidae”
Impacts of Food Limitation on Resistance of <i>Bombus impatiens</i> (Hymenoptera: Apidae) to the Gut Parasite <i>Crithidiai</i> (Trypanosomatida: Trypansomatidae)
<p>Data and R scripts for Conroy et al. experiment testing effects of nectar and pollen limitation on parasite load and survival of bumble bees (Bombus impatiens) infected with Crithidia</p>
Fig. 5 in Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
Fig. 5. kDNA minicircle amplicons presented by all KP1(+) and KP1(–) Trypanosoma rangeli strains examined in this study, and obtained by polymerase chain reaction using the primers 121/122. (M) molecular marker (100-bp DNA ladder).
Fig. 4 in Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
Fig. 4. Isoenzyme profiles at IDH locus presented by KP1(+) and KP1(–) Trypanosoma rangeli strains and T. lewisi.
Fig. 3 in Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
Fig. 3. Diagrammatic representation of the electrophoretic patterns of malate dehydrogenase (MDH), phosphoglucomutase (PGM), glucose phosphate isomerase (GPI) and malic enzyme (ME) displayed by KP1(–) and KP1(+) Trypanosoma rangeli strains, and T. lewisi.
Fig. 1 in Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
Fig. 1. Camera lucida drawings of representative bloodstream trypomastigotes of Trypanosoma rangeli KP1(–) and KP1(+) strains from experimentally infected mice by metacyclic trypomastigotes grown in DMEM medium. The position of the nucleus in each trypomastigote is indicated by arrowheads. Parasites were from Giemsa-stained smears of each strain, as seen under optical microscopy (×1,000). In a trypomastigote of the strain SC-61 are indicated the reference points for taking measurements: anterior end (A), posterior end (P), nucleus (N) and free flagellum (F).
Fig. 2 in Peculiarities of Trypanosoma rangeli KP1(-) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
Fig. 2. Comparative growth in axenic cultures of Trypanosoma rangeli strains [KP1(–) and KP1(+)] and T. lewisi. Data (106 cells/μL) are the averages and the maximum growth from the values taken at the 4th, 7th, 10th, 13th, 17th and 20th days of cultivation in liver infusion-tryptose broth supplemented with 20% fetal calf serum (LIT-20) at 27.3 ± 0.4°C.
Fig. 2. Multiplex PCR gel showing the 716 in Molecular diagnostics of the honey bee parasites Lotmaria passim and Crithidia spp. (Trypanosomatidae) using multiplex PCR
Fig. 2. Multiplex PCR gel showing the 716 to 724 bp amplicon for Lotmaria passim and Crithidia species, the L. passim specific 499 bp amplicon, and the Crithidia specific 245 bp amplicon.
Fig. 1 in Molecular diagnostics of the honey bee parasites Lotmaria passim and Crithidia spp. (Trypanosomatidae) using multiplex PCR
Fig. 1. Bayesian molecular phylogenetic tree showing relationship of 2 Hawaiian Lotmaria passim positive samples relative to other trypanosomes from Gen- Bank for a 608 bp region of the rDNA SSU gene.
Figure 3 in Ecological aspects and molecular detection of Leishmania DNA (Kinetoplastida: Trypanosomatidae) in phlebotomine sand flies (Diptera: Psychodidae) from a rural settlement in the Eastern Amazon, Brazil
Figure 3 Abundance of Phlebotominae Sand flies from Perimetral Norte Rural Settlement, Pedra Branca Municipality, Amapá State, Brazil, collected from February 2018 to February 2019, at the collection sites: ID intradomicile, PD peridomicile, F100m forest 100m from edge, F400m forest from edge. The letters "a" and "b" represent the significant difference.
Figure 2 in Ecological aspects and molecular detection of Leishmania DNA (Kinetoplastida: Trypanosomatidae) in phlebotomine sand flies (Diptera: Psychodidae) from a rural settlement in the Eastern Amazon, Brazil
Figure 2 Shannon's Index of Phlebotominae Sand Flies from Perimetral Norte Rural Settlement, Pedra Branca Municipality, Amapá State, Brazil, collected from February 2018 to February 2019, at the collection- sites: ID intradomicile, PD peridomicile, F100m forest 100m from edge, F400m forest from edge. The letters "a" and "b" represent the significant difference.
Fig. 4 in Utilising a novel surveillance system to investigate species of Forcipomyia (Lasiohelea) (Diptera: Ceratopogonidae) as the suspected vectors of Leishmania macropodum (Kinetoplastida: Trypanosomatidae) in the Darwin region of Australia
Fig. 4. Assessment of L. macropodum DNA using FTAṜ card technology. FTAṜ cards were exposed to field-collected F. (Lasiohelea). Cards with and without insects adhered were processed and parasite load was determined with qPCR. 4.83% (7/145) FTAṜ cards were positive for L. macropodum DNA. Black columns show parasite load detected on each positive FTAṜ card (numbered FTA.1 – FTA.7). Dashed columns show the number of insects adhered to each card. When dashed columns are absent, this signifies the absence of insects on the positive cards.
Fig. 1 in Utilising a novel surveillance system to investigate species of Forcipomyia (Lasiohelea) (Diptera: Ceratopogonidae) as the suspected vectors of Leishmania macropodum (Kinetoplastida: Trypanosomatidae) in the Darwin region of Australia
Fig. 1. Wax-paper cups were used to contain and maintain field-collected biting midges. (A) Honey-coated FTAṜ cards were left at room temperature for 48 h allowing even absorption of honey into the cards. (B) A 2.5 cm slit was carved into the bottom of disposable cup and sealed with adhesive tape. (C) Insects were aspirated directly into the bottom of the containers through a small perforation created before field collection. Once biting midges were collected from the macropods, the small perforation was sealed with a rubber plug. Gauze was used as a lid to seal the top of the containers and fastened securely with a rubber band. The honey-coated FTAṜ card was inserted through the bottom slit after insect collection and once again sealed with adhesive tape.
Fig. 2 in Utilising a novel surveillance system to investigate species of Forcipomyia (Lasiohelea) (Diptera: Ceratopogonidae) as the suspected vectors of Leishmania macropodum (Kinetoplastida: Trypanosomatidae) in the Darwin region of Australia
Fig. 2. Leishmania macropodum DNA detection by qPCR. Individual or pools of F. (Lasiohelea) species were assessed for the presence of L. macropodum DNA. Only positive samples are shown, with each pair of columns representing results from one sample. Black columns depict the parasitic load detected and the dashed columns show the number of insects processed in that sample. Asterisks represent groups that contained ≥ 5 × 106 F. (Lasiohelea) parasites.
Fig. 1 in Surveillance and genotype characterization of zoonotic trypanosomatidae in Didelphis marsupialis in two endemic sites of rural Panama
Fig. 1. Map showing the communities of Las Pavas (LP) (top set of images) and Trinidad de Las Minas (TM) (bottom set of images) with the number of opossums captured and infected with T. cruzi in the 3 collection sites in each community. A. Map with the geographic location of the LP and TM communities in the country of Panama. Satellite view of the P: Peridomicile (B), R1: remnant 1 (C) and R2: remnant 2 (D) collection site each with its 4 transects in the LP community. Satellite view of the P: Peridomicile (E), R1: remnant 1 (F) and R2: remnant 2 (G) collection site each with its 4 transects in the TM community.
Figure 3 from: Gómez-Sánchez EF, Ochoa-Díaz-López H, Espinoza-Medinilla EE, Velázquez-Ramírez DD, Santos-Hernandez NG, Ruiz-Castillejos C, Vidal-López DG, Moreno-Rodríguez A, Flores-Villegas AL, López-Argueta E, De Fuentes-Vicente JA (2022) Mini-exon gene reveals circulation of TcI Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) in bats and small mammals in an ecological reserve in southeastern Mexico. ZooKeys 1084: 139-150. https://doi.org/10.3897/zookeys.1084.78664
Figure 3 PCR products of the mini-exon gene in blood of smalls mammals from the "El Zapotal" Ecological Reserve. Amplification resulted in a PCR product of 350 bp and this confirms that these parasites belong to the TCI group. L: ladder; samples: Q positive control (Qro. strain); 1–6 D. marsupialis; 7–10 P. mexicanus; 11 H. desmarestianus; 12 D. mexicana.
Figure 1 from: Gómez-Sánchez EF, Ochoa-Díaz-López H, Espinoza-Medinilla EE, Velázquez-Ramírez DD, Santos-Hernandez NG, Ruiz-Castillejos C, Vidal-López DG, Moreno-Rodríguez A, Flores-Villegas AL, López-Argueta E, De Fuentes-Vicente JA (2022) Mini-exon gene reveals circulation of TcI Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) in bats and small mammals in an ecological reserve in southeastern Mexico. ZooKeys 1084: 139-150. https://doi.org/10.3897/zookeys.1084.78664
Figure 1 Location of the "El Zapotal" Ecological Reserve in southeastern Mexico, note the border of the reserve with the urban area.
Figure 2 from: Gómez-Sánchez EF, Ochoa-Díaz-López H, Espinoza-Medinilla EE, Velázquez-Ramírez DD, Santos-Hernandez NG, Ruiz-Castillejos C, Vidal-López DG, Moreno-Rodríguez A, Flores-Villegas AL, López-Argueta E, De Fuentes-Vicente JA (2022) Mini-exon gene reveals circulation of TcI Trypanosoma cruzi (Chagas, 1909) (Kinetoplastida, Trypanosomatidae) in bats and small mammals in an ecological reserve in southeastern Mexico. ZooKeys 1084: 139-150. https://doi.org/10.3897/zookeys.1084.78664
Figure 2 PCR products of the mini-exon gene in blood of bats from the "El Zapotal" Ecological Reserve. Amplification resulted in a PCR product of 350 bp and this confirms that these parasites belong to the TCI group. L: Ladder; Samples: 1 positive control (Qro. strain); 2–11 A. jamaicensis; 12–14 A. lituratus; 15–16 C. perspicillata; 17–18 S. lilium; 19 G. soricina.
Figure 1 in Ecological aspects and molecular detection of Leishmania DNA (Kinetoplastida: Trypanosomatidae) in phlebotomine sand flies (Diptera: Psychodidae) from a rural settlement in the Eastern Amazon, Brazil
Figure 1 Map of the study area and sand flies collection points, Perimetral Norte Rural Seattlement, Pedra Branca Municipality, Amapá State, Brazil.
Fig. 3 in Utilising a novel surveillance system to investigate species of Forcipomyia (Lasiohelea) (Diptera: Ceratopogonidae) as the suspected vectors of Leishmania macropodum (Kinetoplastida: Trypanosomatidae) in the Darwin region of Australia
Fig. 3. Evaluation of L. macropodum viability on FTAṜ cards and the effect of honey. To determine L. macropodum detection on honeycoated and plain FTAṜ cards, an experiment was designed over a 10-week time course. FTAṜ cards (2.5 × 2.5 cm) were inoculated with 10 6 L. macropodum per card in triplicates and DNA was tested at five time points (week 0, 2, 5, 8 and 10).
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Allen Brain Atlas
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