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20 results for “Tuberaceae”
FIGURE 3 in Revision of the genus Reddellomyces (Tuberaceae): a combination of molecular and morphological analysis provides insights into species diversity
FIGURE 3. Phylogeny of Reddellomyces sp. derived from internal transcribed spacers (ITS) rRNA gene sequences rooted with Choiromyces meandriformis. Species with an asterisk are collected from Mediterranean countries Nodes with asterisks (*) are supported with 100 % Bayesian posterior probabilities and>70 % MP, ML bootstrap values.
FIGURE 2 in Revision of the genus Reddellomyces (Tuberaceae): a combination of molecular and morphological analysis provides insights into species diversity
FIGURE 2. Phylogeny of Reddellomyces sp. derived from nuclear large subunit (28S) rRNA gene sequences rooted with Gymnohydnotrya australiana. Nodes with asterisks (*) are supported with 100 % Bayesian posterior probabilities and>70 % MP, ML bootstrap values.
FIGURE 1 in Revision of the genus Reddellomyces (Tuberaceae): a combination of molecular and morphological analysis provides insights into species diversity
FIGURE 1. Light micrographs of MahSb1-3 truffle ascomata: a, overview of the prospected site; b, ascoma; c, cluster of 8 ascomata; d, cross section of the mature and non mature dried ascoma showing the glebal Trama, labyrinthes and peridium (MahSb1 specimens); e, cross section of fresh fruitbodies (MahSb2 specimens); f, cross section of the gleba with sterile veins (arrowheads); g, asci with 5 immature ascospores and paraphyses (MahSb1 specimens); h, mature ascospore (MahSb1 specimens); i, cross section of the gleba showing the hymenium (He) and paraphyses (Pr) (MahSb1 specimens). Scale bars: b = 1 cm; c = 5 cm; d = 3 mm; f = 4 mm; g = 25 μm; h = 15 μm; i = 100 μm.
FIGURE 4. Distinctive RFLP pattern obtained with pDRAW32 in Revision of the genus Reddellomyces (Tuberaceae): a combination of molecular and morphological analysis provides insights into species diversity
FIGURE 4. Distinctive RFLP pattern obtained with pDRAW32 from in silico digestion of ITS rDNA sequences from representative species. In the computer-simulated digestions, the set of seven enzymes AoxI, BtsCI, FatI, GlaI, HaeIII, Hpy1881, HpyCH4V, LmnI, NIaIII, PasI, StyI and TaqI were used. Lanes labelled MW represent Invitrogen 100 kb ladder.
FIGURE 1 in Tuber mixtecorum (Tuberaceae, Pezizales) a new truffle in the Maculatum clade from Mexico
FIGURE 1. Phylogenetic analysis based on Bayesian maximum likelihood and posterior probability phylogenetic analysis generated from ITS rDNA, showing Tuber mixtecorum within the clade Maculatum.
FIGURE 2 in Tuber mixtecorum (Tuberaceae, Pezizales) a new truffle in the Maculatum clade from Mexico
FIGURE 2. Tuber mixtecorum (Holotype). A. Details of the gleba. B. Ascospore showing the alveolate ornamentation. C. Asci and ascospores. D–E. Details of the terminal elements of the peridium. Scale bar: 10 mm (A); 20 µm (B–E).
FIGURE 2. Choiromyces cerebriformis. a in Choiromyces cerebriformis (Tuberaceae), a new hypogeous species from Yunnan, China
FIGURE 2. Choiromyces cerebriformis. a. The habitat of Choiromyces cerebriformis. b. Fresh ascomata. c. A sectioned ascoma showing the gleba. d. Peridium with two differentiated pseudoparenchymatous layers. e–f. Ascospores. g. SEM photos of ascospores. Scale bars c = 5 cm; d = 50 μm; e–f = 20 μm; g = 2 μm.
FIGURE 2–3 in A new species of Tuber (Tuberaceae, Pezizales) from Inner Mongolia, China
FIGURE 2–3. Ascomata of Tuber mongolicum (2. HMJAU65125; 3. HMJAU65126) on grassland and vertical section of Tuber mongolicum. Bars: a, b=1 cm, Photos by author.
FIGURE 5 in A new species of Tuber (Tuberaceae, Pezizales) from Inner Mongolia, China
FIGURE 5. Tuber mongolicum (HMJAU65125, holotypus!) a. Ascomata and vertical section; b. Asci and Ascospore; c. Peridium. Bars: a=1cm; b=10µm; c=100µm. Drawings by author.
FIGURE 1. MrBayes phylogram inferred from incomplete internal transcribed spacer rDNA sequence data. Choiromyces meandriformis and C in A new species of Tuber (Tuberaceae, Pezizales) from Inner Mongolia, China
FIGURE 1. MrBayes phylogram inferred from incomplete internal transcribed spacer rDNA sequence data. Choiromyces meandriformis and C. alveolatus were used to root the tree. The same outcome was shown via Maximum Likelihood. On each branch, Bayesian posterior probability values (PP> 0.75) and maximum likelihood bootstrap values (MLbs> 75%) are displayed. The new species sequences are denoted by an asterisk (*). Different colours are used to represent the six primary Tuber phylogroups. Our findings establish T. mongolicum sp. nov. based on phylogenetic studies as well as morphological observations.
FIGURE 4 in A new species of Tuber (Tuberaceae, Pezizales) from Inner Mongolia, China
FIGURE 4. Tuber mongolicum (HMJAU65125, holotypus!) a. Peridium; b-c. Asci and Ascospores; d. Ascospore observed under SEM. Bars: a=100µm; b-d=10µm. Photos by author.
Figure 2 from: Guevara-Guerrero G, Bonito G, Smith ME, Healy R, Grupe II AC, Cázares E, Castellano MA, Trappe JM (2018) Tuber aztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales). MycoKeys 30: 61-72. https://doi.org/10.3897/mycokeys.30.22887
Figure 2 a–i Tuber aztecorum (holotype ITCV 993). a; Two ascomata showing the peridial surface (bar = 1 cm) b Ascoma in cross-section showing peridial surface and glebal surface (bar = 1 cm) c Peridial surface magnified showing the verrucose surface (bar = 1 mm) d Clusters of erect hyphae emanating from the peridial surface (bar =10 µm) e A single surface hair-like hypha (bar = 10 µm) f Cystidium (bar = 10 µm) g Cross section of peridium showing pseudoparenchyma-like epicutis (bar = 20 µm) h Ascospores within asci in surface view showing the alveoli (bar = 20 µm) i Ascospore within asci in surface view showing the alveoli magnified (bar = 20 µm).
Figure 1 from: Guevara-Guerrero G, Bonito G, Smith ME, Healy R, Grupe II AC, Cázares E, Castellano MA, Trappe JM (2018) Tuber aztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales). MycoKeys 30: 61-72. https://doi.org/10.3897/mycokeys.30.22887
Figure 1 Phylogenetic tree inferred under the maximum-likelihood (ML) criterion from the ITS rDNA alignment corresponding to the Tuber dataset. The tree was rooted using midpoint rooting. Numbers on the branches represent support values from 1,000 ML bootstrap replicates. The branches are scaled in terms of the expected number of substitutions per site. The phylogeny is rooted with species belonging to the Latisporum clade. Accession numbers in the sequence labels indicate sequences from Genbank.
Figure 5 from: Polemis E, Konstantinidis G, Fryssouli V, Slavova M, Tsampazis T, Nakkas V, Assyov B, Kaounas V, Zervakis GI (2019) Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula. MycoKeys 47: 35-51. https://doi.org/10.3897/mycokeys.47.32085
Figure 5 Phylogenetic tree inferred from Bayesian analysis including 62 ITS sequences assigned to 31 Tuber taxa, including members of major clades of the genus. Sequences are labelled with Latin binomials, GenBank accession numbers and geographic origin. T.pulchrosporum sp. nov. is indicated in boldface. Reference sequences deriving from type material are underlined. Choiromycesalveolatus (Tuberaceae) was used as the outgroup. Bootstrap (BS) values from Maximum Likelihood (ML) analysis (≥ 70%) and Posterior Probabilities (PPs) from Bayesian Inference (≥ 0.95) are shown at the nodes of branches.
Figure 1 from: Polemis E, Konstantinidis G, Fryssouli V, Slavova M, Tsampazis T, Nakkas V, Assyov B, Kaounas V, Zervakis GI (2019) Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula. MycoKeys 47: 35-51. https://doi.org/10.3897/mycokeys.47.32085
Figure 1 T.pulchrosporum sp. nov.: a ascomata in situ (holotype) b ascomata in situ (paratype) c detail of peridium surface (paratype) d section of peridium (paratype).
Figure 2 from: Polemis E, Konstantinidis G, Fryssouli V, Slavova M, Tsampazis T, Nakkas V, Assyov B, Kaounas V, Zervakis GI (2019) Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula. MycoKeys 47: 35-51. https://doi.org/10.3897/mycokeys.47.32085
Figure 2 T.pulchrosporum sp. nov.: a, b peridium structure c, d hair-like hyphae on peridium surface.
FIGURE 2–3 in A new species of Tuber (Tuberaceae, Pezizales) from Inner Mongolia, China
FIGURE 2–3. (Continued) Ascomata of Tuber mongolicum.
Figure 4 from: Polemis E, Konstantinidis G, Fryssouli V, Slavova M, Tsampazis T, Nakkas V, Assyov B, Kaounas V, Zervakis GI (2019) Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula. MycoKeys 47: 35-51. https://doi.org/10.3897/mycokeys.47.32085
Figure 4 T.pulchrosporum sp. nov.: SEM of ascospores.
Figure 3 from: Polemis E, Konstantinidis G, Fryssouli V, Slavova M, Tsampazis T, Nakkas V, Assyov B, Kaounas V, Zervakis GI (2019) Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula. MycoKeys 47: 35-51. https://doi.org/10.3897/mycokeys.47.32085
Figure 3 T.pulchrosporum sp. nov.: asci and ascospores.
FIGURE 1 in Choiromyces cerebriformis (Tuberaceae), a new hypogeous species from Yunnan, China
FIGURE 1. Phylogeny derived from maximum likelihood (ML) analysis of the nrDNA-ITS sequences from the Chroiromyces species, using Dingleya sp. and Labyrinthomyces sp. as the outgroup. Values next to nodes are ML bootstrap support values (BS, left) and Bayesian posterior probabilities (PP, right). Names of novel species and samples with newly generated sequences are shown in bold.
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