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152 results for “U2OS”
Microscope images of human cancer cell lines (U2OS and HL-60)
<p>This is a dataset that contains microscope images from two cell lines, namely, a human osteosarcoma cell line (U2OS) and a human leukemia cell line (HL-60). The dataset was originally prepared for the cell counting task. It contains 165 labeled images (training: 133, test: 32).</p> <p>The file contains three folders:</p> <p>- training: 165 labeled images in .tiff format;<br> - test: 32 labeled images in .tiff format.</p> <p>Each labeled image has the following name: X.Y.N.tiff</p> <p>where:<br> X - the name of the human cancer cell line;<br> Y - a condition identifier (irrelevant);<br> N - the cell count.</p> <p><br> If you use this dataset, please cite the following paper:</p> <ul> <li>Lavitt F, Rijlaarsdam DJ, van der Linden D, Weglarz-Tomczak E, Tomczak JM. Deep Learning and Transfer Learning for Automatic Cell Counting in Microscope Images of Human Cancer Cell Lines. <em>Applied Sciences</em>. 2021; 11(11):4912. https://doi.org/10.3390/app11114912</li> </ul>
2D STORM dataset U2OS Nup96-SNAP A647
<p>2D STORM dataset</p> <p>sample: U2OS Nup96-SNAP cells labelled with A647</p> <p>imaging: STORM, COT buffer, pxlsize 106 nm</p> <p>Cells from: Thevathasan, J.V., Kahnwald, M., Cieśliński, K. <em>et al.</em> Nuclear pores as versatile reference standards for quantitative superresolution microscopy. <em>Nat Methods</em> <strong>16, </strong>1045–1053 (2019) doi:10.1038/s41592-019-0574-9</p>
2D STORM dataset U2OS Nup96-SNAP A647 (Au Fid)
<p>2D STORM dataset</p> <p>sample: U2OS Nup96-SNAP cells labelled with A647</p> <p>imaging: STORM, COT buffer, pxlsize 106 nm</p> <p>drift correction: Au fiducials 100 nm</p> <p>Cells from: Thevathasan, J.V., Kahnwald, M., Cieśliński, K. <em>et al.</em> Nuclear pores as versatile reference standards for quantitative superresolution microscopy. <em>Nat Methods</em> <strong>16, </strong>1045–1053 (2019) doi:10.1038/s41592-019-0574-9</p>
STARR-seq_U2OS-GR
<p>Raw sequencing files for STARR-seq performed in U2OS-GR cells after a 4h dexamethasone treatment (100nM) and vehicle control (ethanol).</p>
DS3b_Thermodynamics_U2OS_RawData
<p>Raw data for thermodynamic analysis (Figs.1,2) in 'Oxaliplatin disrupts nucleolar function through biophysical disintegration' (Schmidt et al., 2022, Cell Reports).</p>
U2OS_p53_Carbon_Tracing
<p>We used mass spectrometry-based metabolite profiling to identify differential utilization of metabolic pathways between U2OS and U2OS<sup><em>p53KO</em></sup> cells. Labeled glucose was used to monitor pathway activity, both at steady state and following exposure to etoposide. </p> <p>Isotope tracing experiments were performed using heavy D-<sup>13</sup>C<sub>6</sub>-Glucose. Cells were seeded on 6-well plates at 200,000 cells per well. After adherence overnight, cells were treated with DMSO or 31.6 µM etoposide. Eight hours prior to the collection of each timepoint, the growth medium was swapped with glucose-free DMEM supplemented with 10 mM D-<sup>13</sup>C<sub>6</sub>-Glucose. At the indicated timepoints, samples were collected in parallel for metabolite extraction or protein quantification. For total protein quantification, cells were trypsinized and pelleted. Pellets were lysed using SDS lysis buffer and quantified using a BCA assay. For extraction of metabolites, growth media was removed, and cells were washed 2 times with ice-cold PBS. With the 6-well plate on dry ice, cells were submerged in 500 µL 80% MeOH. Samples were then incubated at -80°C for 15 minutes. Cell scrapers were used to harvest each sample, and sample wells were washed with an additional 300 µL of 80% MeOH. Samples were vortexed at 4°C for 10 minutes and then centrifuged at top speed for 10 minutes at 4°C. Supernatant was transferred to a new tube and samples were dried using a speed vac. Dried pellets were resuspended in 100 µL of water and vortexed for 10 minutes at 4°C. Samples were then spun for 10 minutes at top speed at 4°C, andsupernatant from each sample was transferred to an LC-MS vial. A QExactive Plus Quadrupole Orbitrap Mass Spectrometer equipped with a HESI II probe (Thermo Fisher Scientific) was then used to perform Mass Spectrometry. Metabolites were quantified by integrating peaks in TraceFinder 5.1 (Thermo Fisher Scientific). Mass tolerance was set to 5 ppm and expected retention times were benchmarked using an in-house library of chemical standards. Natural abundance of heavy isotopes was corrected using IsoCorrectoR (Bioconductor), and each sample was normalized to the amount of total protein. Fold-change and significance was determined using a custom MATLAB script.</p>
PARCLIP analysis of NONO and SFPQ from U2OS and Hela cells
GEO Series GSE113349. Homo sapiens. 4 samples. Type: Other.
Non-synchronized cell cycle transcriptomics in U2OS and HeLa cancer cells
GEO Series GSE104736. Homo sapiens. 15 samples. Type: Expression profiling by high throughput sequencing.
Genome wide expression analysis of ER alpha and beta with or without E2 treatment in U2OS cell line.
GEO Series GSE21769. Homo sapiens. 18 samples. Type: Expression profiling by array.
RNA-seq of U2OS treated with Harmine or INDY and their controls
GEO Series GSE168401. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing.
Nutlin-3a induced changes in the transcriptome of parental and RFX7-/- U2OS cells
GEO Series GSE173483. Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing.
Expression data of CHK1 shRNA knockdown and control shRNA in U2OS cells
GEO Series GSE54267. Homo sapiens. 9 samples. Type: Expression profiling by array.
Transcriptomics analysis of SUMO1 and SUMO2 U2OS knockout (KO) cell lines
GEO Series GSE163884. Homo sapiens. 9 samples. Type: Expression profiling by high throughput sequencing.
Gene expression analysis of mesenchymal stem cells (MSC), osteoblasts and the U2OS (osteosarcoma) cell line
GEO Series GSE27900. Homo sapiens. 8 samples. Type: Expression profiling by array.
Genome-wide analysis of SRC-1/NCOA1 and Erα/ESR1 binding in chromatin obtained from the ERα stably transfected U2OS osteosarcoma cell line (U2OS-ERα) with ChIP-SEQ.
GEO Series GSE26110. Homo sapiens. 7 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Effect of CCAR2 depletion on the gene expression profile of U2OS cells
GEO Series GSE87363. Homo sapiens. 6 samples. Type: Expression profiling by array.
Integrated analysis of H2A.Z isoforms reveals a complex interplay in gene regulation [U2OS_siZ1-siZ2 RNA-seq]
GEO Series GSE131571. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
APA modulator_U2OS_RNA-seq
GEO Series GSE99526. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing.
Expression data in U2OS cells transfected with control-, DBP5-, GLE1- or IPPK-siRNA
GEO Series GSE100424. Homo sapiens. 12 samples. Type: Expression profiling by array.
Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [ACKR3]
GEO Series GSE270077. Homo sapiens. 18 samples. Type: Expression profiling by RT-PCR.
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