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22 results for “Urostylida”
Fig. 1 in Morphology and Molecular Analyses of a New Marine Ciliate, Arcuseries minima sp. nov. (Ciliophora: Urostylidae)
Fig. 1. Arcuseries minima sp. nov. in vivo (A–E) and after protargol impregnation (F, G). (A) Ventral view of a representative specimen. (B, C) Cortical granulation in ventral surface. (D, E) Cortical granulation in dorsal surface, three types of cortical granules: the large (arrow), medium-sized (arrowhead), small (double arrowhead). (F) Ventral view of holotype specimen. (G) Dorsal view of a paratype specimen, arrow indicates a basal body. AZM = adoral zone of membranelles; BC = buccal cirrus; E = endoral; FC = frontal cirri; FTC = frontoterminal cirri; LMC = left marginal cirri; Ma = macronuclear nodules; MC = midventral cirri; Mi = micronuclei; P = paroral; PTC = pretransverse cirri; RMC = right marginal cirri; TC = transverse cirri; 1–3 = dorsal kineties 1–3. Scale bars: 20 µm.
Fig. 1 in Morphology of Two Eschaneustyla Species (Ciliophora, Urostylida), with Notes on Morphogenesis of Eschaneustyla lugeri
Fig. 1. Eschaneustyla terricola (A, B) and E. lugeri (C–E) during interphase, and E. lugeri during morphogenesis (F–L), after protargol impregnation. (A, B) Ventral and dorsal view of same specimen, showing ciliature. Cirri formed from same anlage were connected by solid line. Asterisk denotes the distal end of AZM. (C) Schematic representations of midventral-complex of four E. lugeri specimens with two long midventral rows. Arrows and arrowheads denote midventral rows 1 and 2, respectively. (D, E) Ventral and dorsal view of same specimen, showing typical infraciliature. Cirri formed from same anlage were connected by solid line. Two leftmost cirri develop from anterior part of undulating membranes anlage (dotted line). (F) Ventral view of an early divider that twisted a little at the anterior end, to show the oral primordia of proter (arrow) and opisthe (arrowhead). (G) Enlarged image of proter's oral primordium (arrow) and undulating membranes anlage (arrowhead) of the individual shown F. Note that the former develops in deep of the cortex while the latter develops in the surface. (H) Ventral view of an early divider to show the frontoventral cirral anlagen (arrows). Arrowhead denotes the penultimate frontoventral cirral anlage which forms midventral row 1. Double-arrowhead denotes the frontoventral cirral anlage n that develops into frontoterminal row. Dashed circle represents the flurry area due to the hyperchromatism after protargol impregnation. (I, J) Ventral and dorsal view of an early divider to show the frontoventral cirral anlagen. Arrows and arrowheads in I mark the penultimate and the last frontoventral cirral anlagen, respectively. Note that marginal anlagen and dorsal kineties anlagen (arrows in J) develop intrakinetally. Macronuclear nodules are fusing (arrowheads in J). (K) Ventral view of a middle divider. Arrow marks the partly renewed adoral zone of membranelles of proter. Asterisk denotes two leftmost frontal cirri developed from the undulating membranes anlage. L. Nuclear apparatus of the same specimen as shown in K. Note that macronuclear nodules are fusing into a single mass. AZM, adoral zone of membranelles; CC, caudal cirri; E, endoral; FT, frontoterminal row; LMA, left marginal anlage; LMR, left marginal row; Ma, macronuclear nodules; Mi, micronuclei; MV, midventral row; MV 1, midventral row 1; 1–4, dorsal kineties 1–4; P, paroral; RMA, right marginal anlage; RMR, right marginal row. Scale bars: 100 μm (A, B, D, E); 70 μm (F–L).
Fig. 3 in Morphology and Molecular Analyses of a New Marine Ciliate, Arcuseries minima sp. nov. (Ciliophora: Urostylidae)
Fig. 3. Maximum likelihood and Bayesian inference analyses based on 18S rDNA sequences. The new sequence provided in the present work is indicated in bold and by a white arrow. Numbers at nodes indicate the bootstrap values of ML out of 1,000 replicates and the posterior probability of BI. Fully supported (100/1.00) branches are marked with solid circles. The scale bar corresponds to 2 substitutions per 100 nucleotide positions.
Fig. 2 in Morphology of Two Eschaneustyla Species (Ciliophora, Urostylida), with Notes on Morphogenesis of Eschaneustyla lugeri
Fig. 2. Photomicrographs of Eschaneustyla lugeri (A, B) and E. terricola (D, E) in life and E. lugeri after protargol impregnation (C, F–M). (A, B) Representative individuals. Arrow in A denotes the contractile vacuole. (C) Dorsal side to show the arrangement of extrusomes (arrows). (D) Arrangement of cortical granules. (E) Ventral view. Arrow marks the contractile vacuole. It should be noted that the specimen in E is slightly damaged, thus it doesn't present a narrowly rounded posterior end. (F–H) Ventral views of early dividers, to show the oral primordia of opisthe (F) and proter (arrow in G), the undulating membranes anlage (arrowhead in H) and the frontoventral cirral anlagen (arrow in H). (I) Dorsal view of an early divider to show the intrakinetal development of dorsal kineties anlagen (arrows). (J) Fusion of macronuclear nodules. (K–M) Ventral views of middle dividers. Note that right marginal anlage develops intrakinetally (arrow in K). Arrow in L denotes the formation of the leftmost frontal cirri from frontoventral cirral anlage I. In M, arrow marks the frontoventral cirral anlage n that becomes the frontoterminal row and arrowhead depicts the penultimate frontoventral cirral anlage that forms midventral row 1, respectively. Ma, macronuclear nodules. Scale bars: 100 μm (A, B, E); 40 μm (C, F, G, I); 15 μm (D, J); 25 μm (H, K–M).
Fig. 4 in A New Soil Ciliate, Birojimia soyaensis nov. spec. (Ciliophora: Urostylida) from South Korea
Fig. 4. Phylogenetic tree of 18S rRNA gene sequences, showing the position of Birojimia soyaensis nov. spec. on the basis of Maximum Likelihood (ML) and Bayesian Inference (BI). Bootstrap values for ML and posterior probability values for BI are represented on the inte- rior branches. Black circles indicate the species of the family Holostichidae (sensu Berger 2006 system).
Figs 1A–G in A New Soil Ciliate, Birojimia soyaensis nov. spec. (Ciliophora: Urostylida) from South Korea
Figs 1A–G. Morphology of Birojimia soyaensis nov. spec. from live (A, D–F) and protargol-impregnated (B, C, G) specimens. A, B, E, F – ventral view; C, D, E – dorsal view; B, C – ventral and dorsal views of the holotype specimen: B – arrow indicates pharynx; D – ex- trusive cortical granules (arrow), dorsal cilia (arrowhead); E – arrangement of cortical granules on ventral side; F – arrangement of cortical granules on dorsal side; G – ciliary pattern of cirral rows and dorsal kineties. AZM – adoral zone of membranelles; BC – buccal cirrus; CC – caudal cirri; DK – dorsal kineties; EM – endoral membrane; FC – frontal cirri; FTC – frontoterminal cirri; LMR – left marginal cirral row; Ma – macronuclei; Mi – micronuclei; MP – midventral pairs; PM – paroral membrane; PTC – pretransverse ventral cirri; TC – transverse cirri; 1, inner right marginal cirral row; 2–4, compound rows. Scale bars: 100 μm.
Figs 3A–F in A New Soil Ciliate, Birojimia soyaensis nov. spec. (Ciliophora: Urostylida) from South Korea
Figs 3A–F. Photomicrographs of Birojimia soyaensis nov. spec. from protargol-impregnated specimens. A, B, D – holotype specimen. A – ventral view of specimen; B – anterior portion of ventral view; C – posterior portion of ventral view; D – pharynx; E – anterior portion of dorsal view; F – posterior portion of dorsal view. AZM – adoral zone of membranelles; BC – buccal cirrus; CC – caudal cirri; DK – dorsal kineties; EM – endoral membrane; FC – frontal cirri; FTC – frontoterminal cirri; LMR – left marginal cirral row; Ma – macronuclei; Mi – micronuclei; MP – midventral pairs; PM – paroral membrane; PTC – pretransverse ventral cirri; TC – transverse cirri; 1 – inner right marginal cirral row; 2–4 – compound rows. Scale bars: 100 μm.
Figs 2A–J in A New Soil Ciliate, Birojimia soyaensis nov. spec. (Ciliophora: Urostylida) from South Korea
Figs 2A–J. Photomicrographs of Birojimia soyaensis nov. spec. from live specimens. A, D, E – ventral view; B, C, F, H – dorsal view: B, C – arrows indicate contractile vacuole and collecting canals; D – distribution of cortical granules (arrowhead) and inner right cirral row (arrow); E – distribution of cortical granules on the ventral side, arrowhead indicates a longitudinal row of cortical granules; F – arrows indicate cortical granules on the dorsal side; G – arrow indicates dorsal cilia; H – arrows indicate caudal cirri; I – macronuclear nodules (arrow), micronuclei (arrowhead); J – cortical granules in lateral view (arrow). Scale bars: 100 μm.
Figure 1 in Phylogenetic analyses suggest that Psammomitra (Ciliophora, Urostylida) should represent an urostylid family, based on small subunit rRNA and alpha-tubulin gene sequence information
Figure 1. Morphology and infraciliature of Psammomitra retractilis (F–J, from Song & Warren, 1996). A, B, F, individuals in extended states to show the typical body shapes. Arrowheads in (A) mark the long, dominant membranelles. C, lateral view of a contracted specimen. D, posterior part, to demonstrate the long dorsal cilia. E, anterior part. Arrowheads indicate the long membranelles, whereas arrows mark the dorsal cilia. G, H, dorsal and lateral views of contracted cells. I, J, ventral and dorsal views to show the infraciliature and macronuclear nodules. Scale bars: A, C, D, F = 40 Mm; E = 30 Mm.
Figure 3 in Phylogenetic analyses suggest that Psammomitra (Ciliophora, Urostylida) should represent an urostylid family, based on small subunit rRNA and alpha-tubulin gene sequence information
Figure 3. Maximum parsimony phylogeny of small subunit rRNA genes. Psammomitra is highlighted in black, and holostichids are enclosed in rectangles. Thick branches and arrows denote position of investigated species. Numbers on branches are values generated from 1000 bootstrap replicates.
Figure 2 in Phylogenetic analyses suggest that Psammomitra (Ciliophora, Urostylida) should represent an urostylid family, based on small subunit rRNA and alpha-tubulin gene sequence information
Figure 2. Phylogenetic tree based on small subunit rRNA sequences showing the position of Psammomitra retractilis, by Bayesian inferences applying the GTR + G + I model. '-' reflects disagreement between a method and the reference Bayesian tree at a given node. The fully supported (1.00/100%/100%) branches are marked with solid circles. Psammomitra is shaded black, and holostichids are enclosed in rectangles. Thick branches and arrows denote position of investigated species. The scale bar corresponds to five substitutions per 100 nucleotide positions. Infraciliature of Oxytricha and Uroleptus (from Foissner et al., 2004), Amphisiella (from Li et al., 2007), Trachelostyla (from Gong et al., 2006), and Holosticha (from Hu & Song, 2001) are also shown.
Figure 4 in Phylogenetic analyses suggest that Psammomitra (Ciliophora, Urostylida) should represent an urostylid family, based on small subunit rRNA and alpha-tubulin gene sequence information
Figure 4. Bayesian trees based on different data sets showing phylogenetic relationships amongst Spirotrichea. '-' reflects disagreement between the maximum likelihood/ maximum parsimony method and the reference Bayesian tree at a given node. The fully supported (1.00/100%/100%) branches are marked with solid circles. Species sequenced in the present study are shown in bold type. The scale bar corresponds to 10/2 substitutions per 100 nucleotide positions. A, phylogenetic analyses inferred from alpha-tubulin gene sequences data set. B, phylogenetic analyses inferred from alpha-tubulin amino acids data set.
FIGURE 5. Protargolimpregnated specimens. a in Pseudourostyla pelotensis sp. nov. (Ciliophora, Stichotrichia, Urostylida): a new psammophilic ciliate from the southern Brazil
FIGURE 5. Protargolimpregnated specimens. a. Ventral side; b. Dorsal side; c. Extrusomes (arrows); d. Oral apparatus and macronuclear nodules. Arrow points to bowshaped structure associated to the undulating membranes; e. Formation of the new adoral membranelles during reoganizational morphogenesis (arrow); f. Ventral side of specimen with a shortened left marginal row (arrows). aC—anterior corona; AZM—adoral zone of membranelles; BC—buccal cirrus; DK —dorsal kineties; eM—endoral membrane; FTC—frontoterminal cirri; LMR—left marginal cirral row; Ma — macronuclear nodules; MVR—midventral cirral row; pC—posterior corona; pM— paroral membrane; RMR— right marginal cirral rows; TC— transverse cirri. Scale bars: 10 µm.
FIGURE 2 in Pseudourostyla pelotensis sp. nov. (Ciliophora, Stichotrichia, Urostylida): a new psammophilic ciliate from the southern Brazil
FIGURE 2. Schematic drawings of protargolimpregnated specimens. a. Ventral region. Arrow shows a variation of the virtual intersection of the undulating membranes; b. Dorsal region showing macronuclear nodules. Chromatin granulations are depicted in the two macronuclear nodules pointed by arrows. Asterisk marks the bowshaped structure, which is visible at the macronucleus focal plane; c. Dorsal region showing dorsal kineties (arrow) and marginal cirral rows. LMR — left marginal cirral rows; Ma — macronuclear nodule; RMR — right marginal cirral rows. Scale bars: 10 µm.
FIGURE 1 in Pseudourostyla pelotensis sp. nov. (Ciliophora, Stichotrichia, Urostylida): a new psammophilic ciliate from the southern Brazil
FIGURE 1. Schematic drawings of Pseudourostyla pelotensis sp. nov. a. Living specimen in ventral view; b. Scheme of living specimen showing the contractile vacuoles and their aspect during pulsation; c. Extrusomes aligned close to the pelicle, as seen in specimens from protargol slides; d. Ventral region of protargolimpregnated specimen; e. Bowshaped structure associated with the undulating membranes. Arrow marks new adoral membranelles in formation. aC — anterior corona; aCV — anterior contractile vacuole; AZM — adoral zone of membranelles; BC — buccal cirrus; BS — bowlike structure; eM — endoral membrane; FTC — frontotermial cirri; LMR — left marginal cirral rows; MVR — midventral cirral row; pC — posterior corona; pCV — posterior contractile vacuole; pM — paroral membrane; RMR — right marginal cirral rows; TC — transverse cirri. Scale bars: 10 µm.
FIGURE 4 in Morphological studies and molecular data on a new marine ciliate, Apokeronopsis sinica n. sp. (Ciliophora: Urostylida), from the South China Sea
FIGURE 4. Unmatched sites from SSrRNA gene sequences alignment. Numbers represent the positions of the nucleotides. Missing sites are compensated by gaps (-). Matched sites are marked with dots (.).
FIGURE 3 in Morphological studies and molecular data on a new marine ciliate, Apokeronopsis sinica n. sp. (Ciliophora: Urostylida), from the South China Sea
FIGURE 3. Comparison of Apokeronopsis species. A. crassa (A, B, from Song et al. 2004), A. wrighti (C, D, from Long et al. 2008), A. antarctica (E, F, from Petz 1995), A. ovalis (G, H, from Shao et al. 2009), A. bergeri (I, J, from Li et al. 2008), and A. sinica (K, L, original), to show the morphology of live cells (A, C, E, G, I, K), infraciliature (B, D, F, H, J, L). Scale bars in (A, B, I, J) =100 µm, in (C–H, K, L) = 60 µm.
FIGURE 2 in Morphological studies and molecular data on a new marine ciliate, Apokeronopsis sinica n. sp. (Ciliophora: Urostylida), from the South China Sea
FIGURE 2. Photomicrographs of Apokeronopsis sinica n. sp. from live cells (A–E, I, J) and after protargol impregnation (K–O), and ventral views of live cells of A. crassa (F), A. bergeri (G) and A. wrighti (H). (A) Ventral view of the typical specimen. (B) Ventral view, showing the contractile vacuole (arrow). (C) Lateral view. (D) Ventral view of the anterior portion, arrows indicate the cilia of adoral zone of membranelles, arrowheads mark the paroral membranes and double-arrowheads depict the buccal cirri. (E) Lateral view to show the large cortical granules distributed on dorsal side. (I) Ventral view, to show the cortical granules: vermeil large (arrows), colorless large (arrowheads) and colorless small ones (double-arrowheads). (J) Ventral view, showing the cortical granules between the cirri rows. (K) infraciliature of the ventral side of a typical specimen. (L) Ventral view of the anterior portion, arrow indicates the paroral membrane and arrowhead marks the endoral membrane. (M) Ventral view of the anterior-right portion, arrowheads refer to frontoterminal cirri, and arrows show the buccal cirri. (N) Macronuclear nodules. (O) Ventral view of the posterior portion, note the transverse cirri (arrows). Scale bars in (A, B, C, F, G, H) = 100 µm, in (K) = 80 µm.
FIGURE 1. Apokeronopsis sinica n in Morphological studies and molecular data on a new marine ciliate, Apokeronopsis sinica n. sp. (Ciliophora: Urostylida), from the South China Sea
FIGURE 1. Apokeronopsis sinica n. sp. from live cells (A–D) and after protargol impregnation (E–F). (A, B) Ventral views of two typical specimens. (C) Dorsal view; to show the sparsely distributed large granules. (D) Details of the cortex, double-arrowheads indicate the smaller cortical granules, arrows mark the vermeil larger ones and arrowheads mark the colorless larger ones. (E, F) Ventral and dorsal views of the infraciliature and nuclear apparatus. AZM = adoral zone of membranelles; BC = buccal cirri; BiC = bicoronal cirri; DK = dorsal kineties; EM = endoral membrane; FTC = frontoterminal cirri; LMR = left marginal row; Ma = macronuclei; MC = midventral complex; PM = paroral membrane; RMR = right marginal row; TC = transverse cirri. Scale bars = 80 µm.
FIGURE 1 in Benthic ciliates from Sepetiba Bay (Rio de Janeiro, Brazil) with description of Pseudokeronopsis sepetibensis n. sp. (Spirotrichea: Urostylida)
FIGURE 1: Map of Sepetiba Bay located in the south region of Rio de Janeiro state, with the circle marks indicates the sampling stations.
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