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210 results for “Wolbachia”
Supplementary data files for Manzano-Marín 2020 "No evidence for Wolbachia as a nutritional co-obligate endosymbiont in the aphid Pentalonia nigronervosa"
<p>Supplementary data for Manzano-Marín 2019 "No evidence for Wolbachia as a nutritional co-obligate endosymbiont in the aphid Pentalonia nigronervosa".</p> <p>The data in "supplementary_data.tar.gz" consists of six folders:</p> <p>1) "Buchnera_GenBank_annotation”: GenBank-formatted file of the annotated genes of <em>Buchnera</em> from <em>Pentalonia nigronervosa</em> (BPn).</p> <p>2) "Buchnera_gene_BLAST_search”: Tabular BLAST output files and FASTA-formatted files of the identified <em>Buchnera</em> Bpn genes.</p> <p>3) "P_nigronervosa_blastx_binning”: Tabular BLAST output files and FASTA-formatted files of the <em>Buchnera</em>, <em>Wolbachia</em>, and mitochondrion bins.</p> <p>4) "P_nigronervosa_BOWTIE_map_vs_bins": BAM-formatted alignment files for read libraries vs. <em>Buchnera</em> and <em>Wolbachia</em> bins from <em>Pentalonia nigronervosa</em>.</p> <p>5) "P_nigronervosa_BOWTIE_map_vs_genes": BAM-formatted alignment files for read libraries vs. genes from <em>Buchnera</em> and <em>Wolbachia</em> bin from <em>Pentalonia nigronervosa</em>.</p> <p>6) "P_nigronervosa_SPAdes_assembly": Output files for pooled SPAdes assembly.</p> <p>Also, the filtered and trimmed FASTQ files used for genome assembly can be found in the comrpessed file "read_files_clean.tar.gz".</p>
Read-mapping for next-generation sequencing data (Wolbachia)
<p>Code, log files and QC data. NCBI SRA accession number SRP091004. Note that sequencing Wolbachia was not a central aim of the project, and was undertaken in order to maximise the amount of information that could be extracted from the raw genome sequence data targetted at the fruit-fly host.</p> <p>The pre-print manuscript for this data is available on biorxiv: "Whole genome resequencing of a laboratory-adapted Drosophila melanogaster population sample" http://biorxiv.org/content/early/2016/10/17/081554 doi: http://dx.doi.org/10.1101/081554</p>
Merged anvio profiles for Wolbachia MAGs from Culex pipiens midgut and ovary samples
<p>Anvi’o merged profile databases for <em>Wolbachia</em> MAGs from <em>Culex pipiens</em> midgut and ovary metagenomes. </p>
Fig. 3 in The Wolbachia endosymbiont of Brugia malayi has an active phosphoglycerate mutase: a candidate target for anti-filarial therapies
Fig. 3 Activity of recombinant wBm–iPGM. Conversion of 3-PG to 2-PG by MBP–wBm–iPGM (filled circle) is measured indirectly by a decrease in NADH concentration, determined spectrophotometrically at 340 nm. Consumption of NADH is directly proportional to PGM activity. A baseline control lacking wBm–iPGM (open circle) is shown
Fig. 1 in The Wolbachia endosymbiont of Brugia malayi has an active phosphoglycerate mutase: a candidate target for anti-filarial therapies
Fig. 1 Alignment of the deduced amino acid sequences of various iPGM enzymes. The sequence of iPGM from the Wolbachia endosymbiont of Brugia malayi (wBm; GenBank accession no. AAW70991) is aligned with the extensively studied and structurally characterized iPGM from Bacillus stearothermophilus (Bs; GenBank accession no. Q9X519; Jedrzejas et al. 2000a,b) and the characterized iPGM from Brugia malayi (Bm; GenBank accession no. AAQ97626; Zhang et al. 2004; Raverdy et al. 2007). Residues that are identical in at least two of the three sequences are shaded in black, while conserved amino acid changes are grey. The catalytic serine (@) and 13 other residues (*) involved in catalysis (Jedrzejas 2000) are conserved in all three enzymes. Alignment generated with ClustalW and displayed with BOXSHADE (www.ch.embnet.org/software/ BOX_form.html)
Fig. 2 in The Wolbachia endosymbiont of Brugia malayi has an active phosphoglycerate mutase: a candidate target for anti-filarial therapies
Fig. 2 Purification of MBP–wBm–iPGM expressed intracellularly in the yeast K. lactis. Fractions from the purification were analyzed by SDS-PAGE and the gel stained with Coomassie Blue. Lane 1 Protein Ladder (New England Biolabs), lane 2 K. lactis lysate, lane 3 supernatant of K. lactis lysate, lane 4 pellet of K. lactis lysate, lane 5 flow-through from amylose column, lane 6 column wash, lanes 7, 8, and 9, elution fractions. The arrowhead indicates the protein band corresponding to recombinant MBP–wBm–iPGM
Fig. 3 in Molecular detection of Wolbachia endosymbiont in reptiles and their ectoparasites
Fig. 3 Phylogenetic relationship of Wolbachia detected in this study (in bold) and other available from GenBank belonging to different supergroups based on a partial sequence of the 16S rRNA gene. Evolutionary analysis was conducted on 1000 bootstrap replications using Maximum Likelihood method and Kimura 2-parameter model with discrete Gamma distribution (+ G) to model evolutionary rate differences among sites selected by best-fit model. GenBank accession number and host species are indicated
Figure 1. Prostaglandin E 2 in The role of a novel Wolbachia (Rickettsiales: Anaplasmataceae) synthetic peptide, WolFar, in regulating prostaglandin levels in the hemolymph of Acheta domesticus (Orthoptera: Gryllidae)
Figure 1. Prostaglandin E 2 activity of Acheta domesticus at different postinjection times relative to the concentrations of WolFar applied. Each point represents the mean ± SE.
Figure 2 in The role of a novel Wolbachia (Rickettsiales: Anaplasmataceae) synthetic peptide, WolFar, in regulating prostaglandin levels in the hemolymph of Acheta domesticus (Orthoptera: Gryllidae)
Figure 2. Formation of nodules in the internal system and fat body of Acheta domesticus following injections of 100% concentration of WolFar. The red triangles indicate the positions of the nodules. A: Negative control; B: at 24 h; C: at 48 h; D: at 72 h.
Figure 2 in Autophagic chemicals effect to Atg8 and rice stripe virus relative expressions, and Wolbachia relative density in Laodelphax striatellus (Hemiptera: Delphacidae)
Figure 2. Comparison of Atg8, Wolbachia, and RSV relative expressions and densities. Vertical bar lines indicate the ± of SD. Significant difference was determined by the Student's t-tests accompanied by Bonferroni correction (0.05). Control was used as a reference. Asterisks represent a significant difference in genes between treatment and control (*p <0.05).
Figure 1 in Autophagic chemicals effect to Atg8 and rice stripe virus relative expressions, and Wolbachia relative density in Laodelphax striatellus (Hemiptera: Delphacidae)
Figure 1. Comparison of Atg8, Wolbachia, and RSV relative expressions and densities. Vertical bar lines indicate the ± of SD. Significant difference was determined by the Student's t-tests accompanied by Bonferroni correction (0.05). Control was used as a reference. Asterisks represent a significant difference in genes between treatment and control (*p <0.05).
Fig. 3 in Mansonella sp. and associated Wolbachia endosymbionts in ring-tailed coatis (Nasua nasua) in periurban areas from Midwestern Brazil
Fig. 3. Phylogenetic tree based on Maximum likelihood method from 16S rRNA of Wolbachia sp. detected in blood samples from coatis (Nasua nasua) from Campo Grande city, Mato Grosso do Sul state, Brazil. Rickettsia sp. was used as outgroup. Analyses were performed based on an alignment of 337 bp and K2P + G4 as evolutionary model.
Fig. 2 in Mansonella sp. and associated Wolbachia endosymbionts in ring-tailed coatis (Nasua nasua) in periurban areas from Midwestern Brazil
Fig. 2. Phylogenetic tree based on Maximum likelihood method from filarioids detected in blood samples from coatis (Nasua nasua) from Campo Grande city, Mato Grosso do Sul state, Brazil. A) cox1 gene, Thelazia callipaeda was used as outgroup (alignment of 681 bp and TIM3+G as evolutionary model); accession numbers from the sequences detected at the present study: OQ261670-OQ261693; B) myoHC gene, Filaria latala and Protospirura muricola were used as outgroups (alignment of 480 pb and HKY + G as evolutionary model); C) hsp70 gene, Protospirura muricola was used as outgroup (alignment of 545 pb and TN + G as evolutionary model).
Fig. 1 in Mansonella sp. and associated Wolbachia endosymbionts in ring-tailed coatis (Nasua nasua) in periurban areas from Midwestern Brazil
Fig. 1. Microfilaria of Mansonella sp. from a coati (Nasua nasua) blood smear stained with Diffy Quicky. Body of the microfilariae with compact column of the nuclei purple in color, sparse osmogenes in the region of the inner body with exception of two big round areas without nuclei (arrow head), with anterior end rounded with short head space and cephalic space with scattered fine nuclei (arrow). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1 in Polymerase chain reaction and gyrA nucleotide sequence analysis of Wolbachia endosymbionts (Rickettsiales: Anaplasmataceae) in various species of Culicidae, Cimex lectularius (Hemiptera: Cimicidae) and Dirofilaria immitis (Rhabditida: Onchocercidae)
Fig. 1. Phylogenetic tree based on Maximum Likelihood depicting the grouping of Wolbachia from various hosts based on analysis of the gyrA gene. The numerical value displayed on branches is the bootstrap value (1,000 replicates), and branches with values below 50% are collapsed. The tree illustrates that gyrA sequences distinguish Wolbachia subtypes based on host taxonomy, demonstrating that this gene may contribute to Wolbachia strain typing projects and future phylogenetic analysis.
Fig. 3 in Two parthenogenetic millipede species/lines of the genus Poratia Cook & Cook, 1894 (Diplopoda, Polydesmida, Pyrgodesmidae) found free from Wolbachia bacteria
Fig. 3. Agarose gel picture showing the results of PCR analysis of Poratia digitata egg DNA using specific WOB76f/WOB1012r primers, to illustrate the presence of eubacteria, but no Wolbachia.
Fig. 1 in Two parthenogenetic millipede species/lines of the genus Poratia Cook & Cook, 1894 (Diplopoda, Polydesmida, Pyrgodesmidae) found free from Wolbachia bacteria
Fig. 1. Agarose gel picture showing the results of PCR analysis of Poratia DNA using primers specific to the wsp gene of Wolbachia, but with traces of a band of the same molecular weight (ca 600 pairs of nucleotides) as in Wolbachia.
Fig. 2 in Two parthenogenetic millipede species/lines of the genus Poratia Cook & Cook, 1894 (Diplopoda, Polydesmida, Pyrgodesmidae) found free from Wolbachia bacteria
Fig. 2. Agarose gel picture showing PCR analysis of Poratia DNA using general eubacterial primers, with a band corresponding to 1459 pairs of nucleotides (eubacteria) being particularly distinct.
Data from: Wolbachia impacts microbiome diversity and fitness-associated traits for Drosophila melanogaster in a seasonally fluctuating environment
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Data from: Transinfection of Wolbachia wAlbB into Culex quinquefasciatus mosquitoes does not alter vector competence for Hawaiian avian malaria (Plasmodium relictum GRW4)
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