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199 results for “airway epithelial cells”
CompareRhythms data in healthy and asthmatic airway epithelial cells
<p><strong>Rationale: </strong>Cellular circadian rhythms regulate multiple cellular processes and have been hypothesized to influence epithelial function in asthma. Airway resistance has circadian variability in patients with asthma, profiling of circadian rhythms of gene expression in human airway epithelia has not been performed. Organotypic cultures of primary human airway epithelial cells differentiated at an air-liquid interface recapitulate the major cell types of human airway epithelia to allow for evaluation of gene expression not possible <em>in vivo</em> and demonstrate rhythmicity of core circadian genes after temperature synchronization.</p> <p><strong>Methods:</strong> Primary human airway epithelial cells from 6 healthy children and 6 children with asthma were differentiated an air-liquid interface and circadian rhythms were synchronized using cycled incubator temperature. RNA was harvested every 4 hours and RNA-sequencing performed to measure transcriptome-wide expression, with differential rhythmicity of genes identified using CompareRhythms.</p> <p><strong>Results:</strong> Core circadian genes <em>ARNTL</em> and <em>NR1D1</em> demonstrated rhythmicity in both healthy and asthmatic airway epithelial cells with maintained phase relationships. In RNAseq data from healthy and asthmatic airway epithelial cells, 646 (4%) of protein-coding genes were rhythmic, with 110 genes exhibiting differential rhythmicity in asthma. Gene set enrichment analysis using EnrichR revealed that genes in circadian rhythm, nuclear receptor, and cell adhesion pathways were rhythmic. Neutrophil chemotaxis, cytokine mediated signaling, and viral protein interactions with cytokine receptor pathways demonstrated differential (gain, loss, or change) rhythmicity in asthma.</p> <p><strong>Conclusions: </strong>Core circadian rhythm genes maintain rhythmicity in healthy and asthmatic human airway epithelia, and regulate cytokine mediated signaling and neutrophil chemotaxis pathways in asthmatic human airway epithelia.</p>
CompareRhythms data in healthy and asthmatic airway epithelial cells
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RNA sequencing of sorted conducting airway epithelial cells from FGF9-overexpressing and control mouse lungs at 1 day post-infection with IAV
<p><span>Influenza A virus (IAV) preferentially infects conducting airway and alveolar epithelial cells in the lung. The outcome of these infections is impacted by the host response, including the production of various cytokines, chemokines, and growth factors. Fibroblast growth factor-9 (FGF9) is required for lung development, can display antiviral activity <em>in vitro</em>, and is upregulated in asymptomatic patients during early IAV infection. We therefore hypothesized that FGF9 would protect the lungs from respiratory virus infection and evaluated IAV pathogenesis in mice that overexpress FGF9 in club cells in the conducting airway epithelium (FGF9-OE mice). However, we found that FGF9-OE mice were highly susceptible to IAV and Sendai virus infection compared to control mice. FGF9-OE mice displayed elevated and persistent viral loads, increased expression of cytokines and chemokines, and increased numbers of infiltrating immune cells as early as 1 day post-infection (dpi). Gene expression analysis showed an elevated type I interferon (IFN) signature in the conducting airway epithelium and analysis of IAV tropism uncovered a dramatic shift in infection from the conducting airway epithelium to the alveolar epithelium in FGF9-OE lungs. These results demonstrate that FGF9 signaling primes the conducting airway epithelium to rapidly induce a localized, protective IFN and proinflammatory cytokine response during viral infection. Although this response protects the airway epithelial cells from IAV infection, it allows for early and enhanced infection of the alveolar epithelium, ultimately leading to increased morbidity and mortality. Our study illuminates a novel role for FGF9 in regulating respiratory virus infection and pathogenesis.</span></p>
In Vitro Evaluation of a Novel Drug on Airway Epithelial Cells Obtained From Participants With Severe Asthma
ClinicalTrials.gov study NCT02740049. IPD Sharing: NO. Countries: 1. Publications: 0.
RNA sequencing of sorted conducting airway epithelial cells from FGF9-overexpressing and control mouse lungs at 1 day post-infection with IAV
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Type II Alveolar Epithelial Cell Aryl Hydrocarbon Receptor Protects Against Allergic Airway Inflammation through Controlling Cell Autophagy
GEO Series GSE205818. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
Gene Expression Effects of IL-13 on Primary Human Airway Epithelial Cells
GEO Series GSE37693. Homo sapiens. 8 samples. Type: Expression profiling by array.
MicroRNA-146a and glucocorticoids in inflamed airway epithelial cells
GEO Series GSE120758. Rattus norvegicus; Macaca mulatta; Homo sapiens; Mus musculus. 12 samples. Type: Non-coding RNA profiling by array.
Effects of Myb shRNA on Airway Epithelial Cells
GEO Series GSE60365. Mus musculus. 12 samples. Type: Expression profiling by array.
Transcriptomic profile of cystic fibrosis airway epithelial cells undergoing repair
GEO Series GSE127696. Homo sapiens. 78 samples. Type: Expression profiling by high throughput sequencing.
Human airway epithelial cells co-treated with IL-4 and IL-13 for seven days.
GEO Series GSE268072. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
Fungal allergen extracts-induced gene expression in airway epithelial cells (NCI-H292)
GEO Series GSE22323. Homo sapiens. 4 samples. Type: Expression profiling by array.
Analysis of Human Airway epithelial cells transcriptome after transfection of small RNAs from miR-34/449 family
GEO Series GSE77251. Homo sapiens. 9 samples. Type: Expression profiling by high throughput sequencing.
Intermittent exposure to whole cigarette smoke alters the differentiation of primary small airway epithelial cells in the air-liquid interface culture
GEO Series GSE135188. Homo sapiens. 36 samples. Type: Expression profiling by high throughput sequencing.
Exposure of bronchial airway epithelial cells to elexacaftor/tezacaftor/ivacaftor, Pseudomonas aeruginosa strain PA14 and outer membrane vesicles
GEO Series GSE268718. Homo sapiens. 60 samples. Type: Expression profiling by high throughput sequencing.
Inflammatory activation of airway epithelial cells by pyocyanin
GEO Series GSE22430. Homo sapiens. 4 samples. Type: Expression profiling by array.
RHINOVIRUS INFECTION OF THE AIRWAY EPITHELIUM ENHANCES MAST CELL IMMUNE RESPONSES VIA EPITHELIAL-DERIVED INTERFERONS
GEO Series GSE206680. Homo sapiens. 123 samples. Type: Expression profiling by high throughput sequencing.
The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Illumina]
GEO Series GSE32139. Homo sapiens. 12 samples. Type: Expression profiling by array.
Human airway epithelial cell response to LPS and house dust mite antigen
GEO Series GSE8190. Homo sapiens. 250 samples. Type: Expression profiling by array.
Cystic fibrosis Airway primary epithelial cells in air-liquid interrface culture show abnormal inflammation and lipid metabolism related RNA expresssion compared to non-CF
GEO Series GSE154802. Homo sapiens. 9 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.