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2 results for “analytical ultracentrifugation”
Analytical ultracentrifugation datasets of NT-2Rep-CT
<p>AUC datasets for "Metastable liquid-liquid phase separation and aging lead to strong processing path dependence in mini-spidroin solutions" publication.</p> <p>Dataset RunId 861 corresponds to Fig. 2a and Fig. 4a and contains information about NT-2Rep-CT sample 20 g/l concentration in 20 mM Tris pH 8.0 and 110 mM Tris pH 7.2 buffers.</p> <p>Datasets RunId 989 – 1032 correspond to Fig. 3.</p> <p>RunId 989, 991, 1000, 1018 and 1026 were measured using different protein concentrations: 989: 0.675, 1.25 and 2.5 g/l; 991: 10, 5 and 20 g/l; 1000: 0.1 and 2.5 g/l.</p> <p>Datasets RunId 1018 – 1024 contain information about time dependence of NT-2Rep-CT sample at 0.05, 0.1 and 1 g/l concentration in 20 mM Tris pH 8.0 buffer. RunId 1018 – first experiment (0 hour), RunId 1020 – after 24 hours, RunId 1022 – after 48 hours and RunId 1024 – after 120 hours.</p> <p>Datasets RunId 1026 – 1032 contain information about time dependence of NT-2Rep-CT sample at 1, 5 and 10 g/l concentration in 20 mM Tris pH 8.0 buffer. RunId 1026 – first experiment (0 hour), RunId 1028 – after 24 hours, RunId 1030 – after 48 hours and RunId 1032 – after 120 hours.</p>
Development of an advanced multiwavelength emission detector for the analytical ultracentrifuge
<p>This is the raw data for the manuscript:</p> <p>Development of an advanced multiwavelength emission detector for the analytical ultracentrifuge.</p> <p>A readme file containing all descriptions can be found in the main folder. All data are sorted according to their appearance in the figures of the main manuscript and electronic supplementary information.</p> <p>Abstract:</p> <p>An advanced design of the analytical ultracentrifuge (AUC) with multiwavelength emission detection (MWE-AUC) is presented which offers outstanding performance concerning the spectral resolution and range flexibility as well as the quality of the data acquired. The excitation by a 520 nm laser is complemented with a 405 nm laser. An external spectrograph with three switchable tunable gratings permits optimisation of the spectral resolution in an order of magnitude range while keeping the spectral region broad. The new system design leads also to a significant reduction of systematic signal noise and allows the assessment and control of inner filter effects. Details regarding the very large signal dynamic range are presented, an important aspect when studying samples in a broad concentration range of up to five orders of magnitude. Custom-made mono-sector sample cells with 3 mm titanium centerpiece have been developed and are applied as default system components. Our system is validated by complementary studies on two biological systems, fluorescent bovine serum albumin and green fluorescent protein, using the commercial Optima AUC with absorbance detection for comparison. Finally, we demonstrate the capabilities of our second generation MWE-AUC with respect to multiwavelength characterisation of gold nanoclusters, which exhibit specific fluorescence depending on their structure. Overall, this work depicts an important step-stone for the future in-depth studies of size-, shape- and composition-dependent multiwavelength emission properties of colloids.</p>
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