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18 results for “ant fungi”

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zenodo44/100

Data - Ant identity determines the fungi richness and composition of a myrmecochorous seed

<p>Data set and analyse used in the manuscript title "<span>Ant </span><span>identity determines the fungi richness and composition of myrmecochorous seeds". In this manuscript w<span>e explore the effects of seed manipulation on fungi communities promoted by two ants with contrasting effects on seed germination and antimicrobial strategies. We hypothesize that i) seeds manipulated by <em>Atta sexdens</em> (increase seed germination and has broad cleaning strategies) will present lower fungi richness than those manipulated by <em>Acromyrmex subterraneus</em> (impair seed germination and has narrow cleaning strategies); <span>ii) seeds manipulated by </span><em>A. sexdens </em>and<em> Ac. subterraneus </em>will present<em> </em>dissimilar<em> </em><span>fungi composition. </span>We tested the hypotheses by identifying fungi morphotypes present in three groups of seeds: i) manipulated by <em>Atta sexdens</em>; ii) manipulated by <em>Ac. subterraneus</em>; iii) unmanipulated. </span></span><span>From the seeds manipulated by ants, we randomly take a sub-sample of 20 seeds per nest to evaluate the fungi community. We also took 20 unmanipulated seeds (the ones left outside each experimental nest). Therefore, we had three seed treatment groups: <span><span>&nbsp;</span></span>i) manipulated by <em>A. sexdens</em> (20 seeds per nest = 80 seeds)<em>;</em> ii) manipulated by <em>Ac. Subterraneus</em> (20 seeds per nest = 80 seeds)<em> </em>and iii) control - unmanipulated seeds left outside of each experimental nest (20 seeds outside of each nest = 160 seeds).</span><span>To allow the fungi growth on seeds, we placed each seed separately on sterile Petri dishes (90 x15 mm) filled with 15 ml of Potato-Dextrose-Agar (PDA) culture medium. We then transported each Petri dish to a Bio-Oxygen-Demand incubator (BOD) at 25&deg;C for 28 days. After that period, we sampled the fungi and prepared microscope slides for each fungus morphotype found in each Petri dish. We identified the fungi to the lower taxonomic level possible using &ldquo;The genera of Hyphomycetes&rdquo; <span><span>(Seifert et al. 2011)</span></span> and the website mycobank.org . We used this method because it is widely used to identify pathogens in seeds, has a low cost and has good specificity to identify fungi<span>&nbsp;</span>. Furthermore, PDA medium is a non-selective fungi growth media suitable for a broad range of fungi species.</span></p> <p><span><span>&nbsp;</span></span></p>

opencc-by-4.0Jan 2024View details →
dryad40/100

Ant handling changes myrmecochore seed coat microbiomes and alters diversity of seed-borne plant pathogenic fungi

<p>The putative benefits to seeds in myrmecochory (ant-mediated seed dispersal) are often cast in a reward context. However, microbes have been mostly overlooked as seed mortality agents in myrmecochory, as have potential treatments provided by ant-handling. We investigated the effects of ant handling on the diversity of seed coat fungal communities of three myrmecochorous plant species. Ant-handling altered measures of both alpha and beta diversity of fungal communities. Ant-handled seeds harbored different overall fungal communities and plant pathogen communities than non-ant-handled seeds. The myrmecochore pathogenic fungal community showed high dissimilarity (high pairwise community turnover) between ant-handled and control seeds, while beta diversity measures for ant-handled seeds and seeds with manually-removed elaiosomes were less dissimilar. Ant handling may offer an additional benefit to myrmecochorous seeds via the reduction of the seed coat pathogenic community, which may be driven by elaiosome removal or as a byproduct of ant cleaning behaviors and chemical secretions. </p>

opencc-zeroJan 2024View details →
dryad40/100

Ant handling changes myrmecochore seed coat microbiomes and alters diversity of seed-borne plant pathogenic fungi

Open the record for dataset details and reuse information.

publicJan 2024View details →
zenodo32/100

FIGURE 8 in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 8. Scanning Electron Micrographs (SEM) of the infected ants. a) C. bispinosus infected by O. camponoti-bispinosi; b) Close-up of the O. camponoti-bispinosi ascoma; c) close-up of the O. camponoti-atricipis ascoma; d) infected C. atriceps; e) infected C. indianus; f) close-up of O. camponoti-indiani ascoma. Images: João Araújo.

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 6. Infected Ophiocordyceps camponoti-atricipis showing initial development. a in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 6. Infected Ophiocordyceps camponoti-atricipis showing initial development. a) Day 1 (24th March 2011): Ant attaching to the leaf and dying a few hours later; b) Day 3: Cottony white fungal mycelium arises from ant sutures and joints, the stroma emerges from behind the ant head; c) Day 5: the covering mycelium becomes light brown and the pink-tipped stroma continues to grow. In 2–3 weeks, the ascoma forms and matures over time depending on climatic conditions. Images: João Araújo.

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 7 in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 7. Unusual aggregation of different ant species biting on the same leaf and even onto the stoma from another infected ant. Arrows show the four different ants (two species) dead at the same spot. Image: João Araújo.

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 4. Ophiocordyceps camponoti-indiani a in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 4. Ophiocordyceps camponoti-indiani a) Camponotus indianus biting into a leaf, several stromata arising from dorsal pronotum, mesonotum and leg joints, with a characteristic purplish coloration. a-1) lateral, fertile cushion (ascoma); a-2) Close up of the dead ant's head showing the biting behavior. b) Section through ascoma showing perithecial arrangement (bar = 500 μm); c) Ascospore after 24 h, with very long capilliconidiophores (1-3) with capilliconidia at the tip (bar = 50 μm); c-1) Detail of fusoid capilliconidium (bar = 10 μm); d) Close up of perithecia showing asci arrangement and the semi-erumpent ostiole (bar = 50 μm); e) Ascus showing the spiral arrangement of ascospores (bar = 20 μm); e-1) Ascus cap detail (bar = 5 μm); f) Section of upper part of stroma showing asexual morph (Hirsutellalike A type), with long-necked phialides (bar = 10 μm); g) Phialides formed as mycelial cushions (sporodochia) on leg joints and antenna (Hirsutella-like C type) (bar = 10 μm). Images: João Araújo.

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 2. Ophiocordyceps camponoti-atricipis a in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 2. Ophiocordyceps camponoti-atricipis a) Single stroma, characteristic of Ophiocordyceps unilateralis sensu lato, with a single lateral ascoma, arising anteriorly from pronotum of Camponotus atriceps, firmly attached to the edge of the leaf (bar = 3 mm); b) Detail of fertile region (ascoma) (bar = 0.8 mm); c) Section through ascoma showing the mainly immersed perithecial arrangement (bar = 200 μm); d) Ascospore with a needle-like outgrowth (capilliconidiophore) producing terminal conidium (bar = 20 μm); e) Close-up of conidium (bar = 10 μm); f) Close-up of perithecium (bar = 50 μm); g) Ascus, clavate in shape and with a prominent cap (bar = 20 μm); h) Section of upper part of stroma showing asexual morph (Hirsutella-like A type), with a palisade of subulate phialides (bar = 10 μm) Images: João Araújo.

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 5. Maximum-likelihood tree obtained from a in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 5. Maximum-likelihood tree obtained from a concatenated dataset of three genes (nu-SSU, nu-LSU, ITS) showing the placement of O.camponoti-atricipis, O. camponoti-bispinosi and O. camponoti-indiani within Ophiocordyceps unilateralis complex and relative to other Ophiocordycipitaceae species. Numbers above branches indicate bootstrap scores&gt;70 (ML/MP).

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 1 in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 1. Map showing the forest reserves sampled in the central Brazilian Amazon: A) Reserva Adolpho Ducke; B) Parque Nacional de Anavilhanas; C) Parque Nacional do Viruá; D) Estação Ecológica de Maracá.

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 3. Ophiocordyceps camponoti-bispinosi a in Unravelling the diversity behind the Ophiocordyceps unilateralis (Ophiocordycipitaceae) complex: Three new species of zombie-ant fungi from the Brazilian Amazon

FIGURE 3. Ophiocordyceps camponoti-bispinosi a) Infected Camponotus bispinosus biting into tip of palm leaf; b) Close-up showing biting behavior; c) Section through ascoma showing perithecial arrangement (bar = 100 μm); d) Ascospore after 48 h germinating with a single capilliconidiophore with a capilliconidium at the tip (bar = 10 μm), upper right corner shows the capilliconidium in close-up (bar = 10 μm); e) Section of perithecium showing the arrangement of asci (bar = 50 μm); f-g) Detail showing the prominent ascus cap (bar = 10 μm); h) Section of the swollen stromatal tip with the asexual morph (Hirsutella-like A) (bar = 10 μm). Images: João Araújo.

opennotspecifiedJul 2015View details →
zenodo32/100

FIGURE 3 in Introducing Ophiocordyceps thanathonensis, a new species of entomogenous fungi on ants, and a reference specimen for O. pseudolloydii

FIGURE 3 Ophiocordyceps pseudolloydii (MFLU 16–2914, reference specimen). a Habitat of Ophiocordyceps pseudolloydii. b Ascostroma emerging from infected ant host. c Fertile head of ascostroma. d Vertical sections showing the completely embedded obliquely aligned perithecia. e Perithecia. f Peridium. g–i Immature to mature asci. j Asci in Indian ink. k Apical cap of ascus. l, m Secondary ascospore. n, o Culture on PDA medium, obverse and reverse view. Scale Bars: b, d–f = 1000 μm, c = 200 μm, g–j = 500 μm, k = 100 μm, l = 20 μm, m = 10 μm, n, o = 20 mm.

opennotspecifiedNov 2017View details →
zenodo32/100

FIGURE 2 in Introducing Ophiocordyceps thanathonensis, a new species of entomogenous fungi on ants, and a reference specimen for O. pseudolloydii

FIGURE 2 Ophiocordyceps thanathonensis (MFLU 16–2908, holotype). a Habitat of Ophiocordyceps thanathonensis. b Ascostroma emerging from infected ants. c, d Overview of host. e Fertile head of ascostroma. f Vertical section of the stroma. g, h Ascomata. i Peridium. j–l Immature to mature asci. m Apical cap of asci. n Parts of ascospores. o–q Secondary ascospore. Scale Bars: d–f = 1000 μm, g = 500 μm, h = 200 μm, i–l = 100 μm, m = 10 μm, n = 20 μm, o–q = 5 μm.

opennotspecifiedNov 2017View details →
zenodo32/100

FIGURE 1 in Introducing Ophiocordyceps thanathonensis, a new species of entomogenous fungi on ants, and a reference specimen for O. pseudolloydii

FIGURE 1. Phylogenetic tree of Ophiocordyceps thanathonensis and O. pseudolloydii generated from maximum likelihood analysis of ITS, SSU, LSU, RPB1, and TEF1α sequence data. Tolypocladium inflatum and T. ophioglossoides were used as outgroup taxa. Maximum likelihood bootstrap values greater than 75% and Bayesian posterior probabilities over 0.95 are indicated above the nodes. The new species were indicated in blue.he ex-types are indicated in bold. The pathogens of ants are marked with ant drawings.

opennotspecifiedNov 2017View details →
zenodo28/100

Supplementary material 1 from: Tang D, Zhao J, Lu Y, Wang Z, Sun T, Liu Z, Yu H (2023) Morphology, phylogeny and host specificity of two new Ophiocordyceps species belonging to the "zombie-ant fungi" clade (Ophiocordycipitaceae, Hypocreales). MycoKeys 99: 269-296. https://doi.org/10.3897/mycokeys.99.107565

Phylogenetic tree of Ophiocordyceps and related genera, based on single gene SSU sequence

opencc-zeroOct 2023View details →
zenodo28/100

Supplementary material 2 from: Tang D, Zhao J, Lu Y, Wang Z, Sun T, Liu Z, Yu H (2023) Morphology, phylogeny and host specificity of two new Ophiocordyceps species belonging to the "zombie-ant fungi" clade (Ophiocordycipitaceae, Hypocreales). MycoKeys 99: 269-296. https://doi.org/10.3897/mycokeys.99.107565

Phylogenetic tree of Ophiocordyceps and related genera, based on single gene TEF1a sequence

opencc-zeroOct 2023View details →
zenodo28/100

Supplementary material 3 from: Tang D, Zhao J, Lu Y, Wang Z, Sun T, Liu Z, Yu H (2023) Morphology, phylogeny and host specificity of two new Ophiocordyceps species belonging to the "zombie-ant fungi" clade (Ophiocordycipitaceae, Hypocreales). MycoKeys 99: 269-296. https://doi.org/10.3897/mycokeys.99.107565

Phylogenetic tree of Ophiocordyceps and related genera, based on single gene RPB1 sequence

opencc-zeroOct 2023View details →
dryad28/100

Data from: Do zombie ant fungi turn their hosts into light seekers?

Open the record for dataset details and reuse information.

publicDec 2018View details →

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