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20 results for “antibody validation”
Antibody Validation for Cyclical Immunofluorescence Microscopy of Human Kidneys (Part 1, Figures 31 and 33)
<p>This dataset includes Cyclical Immunofluorescnce (CyCIF) images (15-20 channels) of frozen human kidney sections interogated with a panel of 12 validated antibodies designed to evaluate renal tubular cell segmentation (Figures 31 and 32), an a panel of 12 validated antibodies and a lectin designed to evaluate glomerular and glomerulus-associated structures in the normal human kidney (Figure 33). We have attached an excel file (Supplemental Table 2) that includes all of the experimental and (de-identified) patient metadata associated with these images, with information and comments about each of the channel images, the antibodies used, CyCIF cycles, and the cell types and extracellular matrix compartments identified with these combinations of antibodies. Because of the sixze of the images, we have divided this into two separate datasets (Antibody Validation for Cyclical Immunofluorescence Parts 1 and 2). </p>
Antibody Validation for Immunofluorescence Microscopy of Human Kidneys (Part 2, Figures 16-30)
<p>This dataset includes multiplex immunofluorescence images (mostly 3+1 channels) of frozen human kidney sections that have been used to validate a panel of 27 antibodies and 1 lectin designed to define the main cellular and extracellular matrix (ECM) comparments in the normal human kidney. We have attached an excel file (Supplemental Table 1) that includes all of the experimental and (de-identified) patient metadata associated with these images with information, and comments about each of the images, the antibodies used, and the cell types and ECM compoartments identified using these antibodies. Because of the size and number of images used for these studies, we have divided this into two separate datasets (Antibody validation studies Parts 1 and 2). </p> <p>A subset of these antibodies have also been evaluated for both 2D and 3D cyclical immunofluorescence studies that have been included in separate datasets under this umbrella "community". These are identified in the "Antibodies used" tab in Supplemental Table 1 (CyCIF Cycles). </p>
Antibody Validation for Immunofluorescence Microscopy of Human Kidneys (Part 1, Figures 1-15)
<p>This dataset includes multiplex immunofloresence images (mostly 3+1 channels) of frozen human kidney sections that have been used to validate a panel of 27 antibodies and 1 lectin designed to define the main cellular and extracellular matrix compartments in the normal human kidney. We have attached an excel file (Supplemental Table 1) that includes all of the experimental and (deidentified) patient metadata associated with these images with information and comments about each of the images, the antibodies used, and the cell types and ECM compartments identified using these antibodies. Because of the size and number of images used for these validation studies, we have divided this into two separate datasets (Antibody validation studies Parts 1 and 2). </p> <p>A subset of these antibodies have also been evaluated for both 2D and 3D cyclical immunofluoresecnce studies that have been included in separate datasets under this umbrella "community". These are identfied in the "Antibodies used" tab in Supplemental Table 1 (CyCIF Cycles). </p> <p> </p> <p> </p> <p> </p>
Validation of a SARS-CoV-2 surrogate neutralization test detecting neutralizing antibodies against the major variants of concern
<p>SARS-CoV-2 infection and/or vaccination elicit a broad range of neutralizing antibody responses against the different variants of concern (VOC). We established a new variant-adapted surrogate neutralization test (sVNT) and assessed the neutralization activity against the ancestral B.1 (WT) as well as VOC Delta, Omicron BA.1, BA.2 and BA.5. Performances were compared to the reference virus neutralization test (VNT) against the respective VOC using three different cohorts collected during the COVID waves. Pre-COVID samples confirmed 100% specificity of the sVNT. Correlation analyses showed moderate to strong correlation for Omicron sub-variants (Spearman’s r=0.7081 for BA.1, r=0.7205 for BA.2 and r=0.6042 for BA.5), and for WT (r=0.8458) and Delta-sVNT (r=0.8158), respectively. Comparison of the WT-sVNT performance with two CE-IVD commercial kits “Icosagen SARS-CoV-2 Neutralizing Antibody ELISA kit” and “Genscript cPass kit” revealed an overall good correlation ranging from 0.8673 to -0.8773 and a midway profile between both commercial kits with 87.76% sensitivity and 90.48% clinical specificity resulting in a Youden Index of 78.24. This midway trend was further confirmed on 100 double-vaccinated individuals. The BA.2-sVNT performance was similar to that of the Genscript test. Finally, sVNT ability to assess neutralizing antibodies against Omicron BA.5 was validated on a double-vaccinated cohort (n=100) and an Omicron-breakthrough infection cohort (n=91). Correlation analysis revealed a strong association (r=0.8583) between BA.5-sVNT and VNT. Accurate classification was confirmed by receiving operating characteristic (ROC) analysis reporting an area under the curve (AUC) of 0.9543. In conclusion, the sVNT allows for efficient prediction of immune protection against the various VOCs.</p>
Immunohistochemistry validation by comparison of two antibodies
<p><strong><span>Supplementary figure 1. Immunohistochemistry validation by comparison of two antibodies. </span></strong><span>The panels show immunostaining results obtained by two independent TFF1 antibodies. Using MSVA-482M, a distinct cytoplasmic staining was seen in surface epithelial cells (but not of glands) in the stomach (A), subsets of goblet cells in the duodenum (B) and the colon (C), a subset of mucinous cells in the submandibulary gland (D), a subset of luminal epithelial cells and intraluminal mucus in the breast (E), a small subset of urothelial cells (mostly umbrella cells) in the renal pelvis (F), a large subset of urothelial cells in an inflamed urinary bladder (G), and in epithelial cells of the gallbladder (H). Using clone EPR3972, a comparable staining was seen in the stomach (a), the duodenum (b), the colon (c), the submandibulary gland (d), the breast (e), the renal pelvis (f), the bladder (g), and the gallbladder (h). The images A-H and a-h are from consecutive tissue sections.</span></p>
Antibody Validation for Cyclical Immunofluorescence of Human Kidneys (Part 2, Figure 32)
<p>This dataset includes Cyclical Immunofluorescnce (CyCIF) images (15-20 channels) of frozen human kidney sections interogated with a panel of 12 validated antibodies designed to evaluate renal tubular cell segmentation (Figures 31 and 32), an a panel of 12 validated antibodies and a lectin designed to evaluate glomerular and glomerulus-associated structures in the normal human kidney (Figure 33). We have attached an excel file (Supplemental Table 2) that includes all of the experimental and (de-identified) patient metadata associated with these images, with information and comments about each of the channel images, the antibodies used, CyCIF cycles, and the cell types and extracellular matrix compartments identified with these combinations of antibodies. Because of the sixze of the images, we have divided this into two separate datasets (Antibody Validation for Cyclical Immunofluorescence Parts 1 and 2). </p>
Verifying Antibodies After Live Immunization Delivery (VALID): A Study of Measles Vaccine Immunogenicity in Children With Sickle Cell Disease
ClinicalTrials.gov study NCT07356050. IPD Sharing: NO. Countries: 3. Publications: 0.
COVID-19 in Baselland: Investigation and Validation of Serological Diagnostic Assays and Epidemiological Study of Sars-CoV-2 Specific Antibody Responses
ClinicalTrials.gov study NCT04483908. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Comparison of OraQuick HCV Rapid Antibody Test and Standard Serologic Screening for Hepatitis C: Validity, Acceptability and Impact on Linkage to Care
ClinicalTrials.gov study NCT02084719. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Safety and Validation of Efficacy of Oral Administration of the Food Additive Colostrum Derived Anti Clostridium Difficile Antibodies
ClinicalTrials.gov study NCT00747071. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Validation of an Immunochromatographic Assay for IgG/IgM Antibodies to 2019- nCoV
ClinicalTrials.gov study NCT04434417. IPD Sharing: YES. Countries: 1. Publications: 0.
Baseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation Total Ig 1:200]
GEO Series GSE42179. Quercus alba; Bothrops moojeni; Pachymenia carnosa; Medicago sativa; Gallus gallus; Megathura crenulata; Rhodotorula; Bos taurus; Ovis aries; Mus musculus; Frangula alnus; Senegalia senegal; Dermestes lardarius; Gossypium hirsutum; Pandalus borealis; Homo sapiens. 113 samples. Type: Protein profiling by protein array.
Baseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation IgM 1:50]
GEO Series GSE42175. Pandalus borealis; Ovis aries; Frangula alnus; Senegalia senegal; Dermestes lardarius; Gossypium hirsutum; Rhodotorula; Homo sapiens; Bos taurus; Mus musculus; Quercus alba; Megathura crenulata; Medicago sativa; Gallus gallus; Bothrops moojeni; Pachymenia carnosa. 113 samples. Type: Protein profiling by protein array.
ChIP-seq validation of recombinant antibodies to histone post-translational modifications
GEO Series GSE66530. Homo sapiens. 10 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Interactive design and validation of antibody panels using single-cell RNA-seq atlases
Open the record for dataset details and reuse information.
Validation of Self Testing Using the INSTI Multiplex HIV1/2 Syphilis Antibody Test
ClinicalTrials.gov study NCT06953024. IPD Sharing: Not stated. Countries: 0. Publications: 0.
Baseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation IgG 1:50]
GEO Series GSE42163. Bothrops moojeni; Pachymenia carnosa; Medicago sativa; Gallus gallus; Ovis aries; Megathura crenulata; Frangula alnus; Rhodotorula; Bos taurus; Mus musculus; Senegalia senegal; Dermestes lardarius; Quercus alba; Gossypium hirsutum; Pandalus borealis; Homo sapiens. 113 samples. Type: Protein profiling by protein array.
Baseline serum anti-glycan antibodies of 113 subjects in a clinical trial of PROSTVAC-VF, a therapeutic cancer vaccine [Validation Total Ig 1:50]
GEO Series GSE42176. Quercus alba; Pandalus borealis; Bothrops moojeni; Senegalia senegal; Pachymenia carnosa; Dermestes lardarius; Gossypium hirsutum; Rhodotorula; Homo sapiens; Bos taurus; Mus musculus; Medicago sativa; Gallus gallus; Ovis aries; Megathura crenulata; Frangula alnus. 113 samples. Type: Protein profiling by protein array.
Spatial transcriptomic validation of a biomimetic model of fibrosis enables re-evaluation of a therapeutic antibody targeting LOXL2
GEO Series GSE255705. Homo sapiens. 48 samples. Type: Expression profiling by high throughput sequencing.
A Study to Validate a New Oral Fluid/Saliva Antibody Test for Novel H1N1v Influenza
ClinicalTrials.gov study NCT01192958. IPD Sharing: Not stated. Countries: 0. Publications: 0.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.