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61 results for “autofluorescence”
Autofluorescence-Free In Vivo Imaging Using Polymer-Stabilized Nd3+-Doped YAG Nanocrystals
<p>Neodymium-doped yttrium aluminum garnet (YAG:Nd<sup>3+</sup>) has been widely developed during roughly the last sixty years and has been an outstanding fluorescent material. It has been considered as the gold standard among multipurpose solid-state lasers. Yet, the successful downsizing of this system into the nano regimen has been elusive, so far. Indeed, the synthesis of a garnet structure at the nanoscale, with enough crystalline quality for optical applications was found to be quite challenging. Here, we present an improved solvothermal synthesis method producing YAG:Nd<sup>3+</sup> nanocrystals of remarkably good structural quality. Adequate surface functionalization using asymmetric double-hydrophilic block copolymers, constituted of a metal-binding block and a neutral water soluble block, provides stabilized YAG:Nd<sup>3+</sup> nanocrystals with a long term colloidal stability in aqueous suspensions. These newly stabilized nanoprobes keep the spectroscopic quality (long lifetimes, narrow emission lines, and large Stokes shift) characteristic of bulk YAG:Nd<sup>3+</sup>. The narrow emission lines of YAG:Nd<sup>3+</sup> nanocrystals are exploited by differential infrared fluorescence imaging, thus achieving an autofluorescence-free <em>in vivo</em> readout. In addition, nanothermometry measurements, based on the ratiometric fluorescence of the stabilized YAG:Nd<sup>3+</sup> nanocrystals, are demonstrated. The progress here reported paves the way for the implementation of this new stabilized YAG:Nd<sup>3+</sup> system in the preclinical arena.</p>
Assessment of skin autofluorescence and its association with glycated hemoglobin, cardiovascular risk markers and concomitant chronic diseases in children with type 1 diabetes
<p>This is the dataset for the publication "Assessment of skin autofluorescence and its association with glycated hemoglobin, cardiovascular risk markers and concomitant chronic diseases in children with type 1 diabetes".</p>
Raw and processed autofluorescence neuroimaging data
<p>Raw and processed <em>in vivo</em> mouse whole-cortex autofluorescence imaging data.</p> <p>This release accompanies the pub <a href="https://doi.org/10.57844/arcadia-b963-15ac">"Label-free neuroimaging in mice captures sensory activity in response to tactile stimuli and acute pain</a>.</p> <p>The raw data is split into five zipfiles because it is quite large (70GB total). Each of these five zipfiles corresponds to one of the five subdirectories in the single zipfile of processed data.</p>
The near‐infrared autofluorescence fingerprint of the brain
<p>The brain is a vital organ involved in most of the central nervous system disorders. Their diagnosis and treatment require fast, cost‐effective, high‐resolution and high‐sensitivity imaging. The combination of a new generation of luminescent nanoparticles and imaging systems working in the second biological window (near‐infrared II [NIR‐II]) is emerging as a reliable alternative. For NIR‐II imaging to become a robust technique at the preclinical level, full knowledge of the NIR‐II brain autofluorescence, responsible for the loss of image resolution and contrast, is required. This work demonstrates that the brain shows a peculiar infrared autofluorescence spectrum that can be correlated with specific molecular components. The existence of particular structures within the brain with well‐defined NIR autofluorescence fingerprints is also evidenced, opening the door to in vivo anatomical imaging. Finally, we propose a rational selection of NIR luminescent probes suitable for low‐noise brain imaging based on their spectral overlap with brain autofluorescence.</p>
Development of low-cost instrumentation for single point autofluorescence lifetime measurements
<p>Dataset used for analysis in the paper. ABSTRACT: Autofluorescence lifetime measurements, which can provide label-free readouts in biological tissues, contrasting e.g. different types and states of tissue matrix components and different cellular metabolites, may have significant clinical potential for diagnosis and to provide surgical guidance. However, the cost of the instrumentation typically used currently presents a barrier to wider implementation. We describe a low-cost single point time-resolved autofluorescence instrument, exploiting modulated laser diodes for excitation and FPGA-based circuitry for detection, together with a custom constant fraction discriminator. Its temporal accuracy is compared against a “gold-standard” instrument incorporating commercial TCSPC circuitry by resolving the fluorescence decays of reference fluorophores presenting single and double exponential decay profiles. To illustrate the potential to read out intrinsic contrast in tissue, we present preliminary measurements of autofluorescence lifetime measurements of biological tissues <em>ex vivo.</em> We believe that the lower cost of this instrument could enhance the potential of autofluorescence lifetime metrology for clinical deployment and commercial development.</p>
Characterization of NAD(P)H and FAD autofluorescence signatures in an isolated-perfused rat heart model
<p>Raw data concerning publication titled "Characterization of NAD(P)H and FAD autofluorescence signatures in an isolated-perfused rat heart model"</p> <p>Abstract</p> <p>Autofluorescence spectroscopy is a promising label-free approach to characterize biological samples with demonstrated potential to report structural and biochemical alterations in tissues in a number of clinical applications. We report a characterization of the ex vivo autofluorescence fingerprint of cardiac tissue, exploiting a Langendorff-perfused isolated rat heart model to induce physiological insults to the heart, with a view to understanding how metabolic alterations affect the autofluorescence signals. Changes in the autofluorescence intensity and lifetime signatures associated with reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) were characterized during oxygen- or glucose-depletion protocols. Results suggest that both NAD(P)H and FAD autofluorescence intensity and lifetime parameters are sensitive to changes in the metabolic state of the heart owing to oxygen deprivation. We also observed changes in NAD(P)H fluorescence intensity and FAD lifetime parameter on reperfusion of oxygen, which might provide information on reperfusion injury, and permanent tissue damage or changes to the tissue during recovery from oxygen deprivation. We found that changes in the autofluorescence signature following glucose-depletion are, in general, less pronounced, and most clearly visible in NAD(P)H related parameters. Overall, the results reported in this investigation can serve as baseline for future investigations of cardiac tissue involving autofluorescence measurements.</p>
Near Infrared Autofluorescence (NIRAF) Detection for Identifying Parathyroid Glands During Parathyroidectomy
ClinicalTrials.gov study NCT05152927. IPD Sharing: NO. Countries: 1. Publications: 3.
Dual-Channel Near-Infrared Autofluorescence Imaging and AI Analysis to Locate Parathyroid Glands (PTFinder)
ClinicalTrials.gov study NCT07010380. IPD Sharing: YES. Countries: 1. Publications: 1.
Skin Autofluorescence as a Risk Marker in People Receiving Dialysis.
ClinicalTrials.gov study NCT02878317. IPD Sharing: NO. Countries: 1. Publications: 17.
Narrow-band Imaging, Autofluorescence Imaging and Gastroesophageal Reflux Disease
ClinicalTrials.gov study NCT01504971. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Evaluating Impact of Near Infrared Autofluorescence (NIRAF) Detection for Identifying Parathyroid Glands During Parathyroidectomy
ClinicalTrials.gov study NCT05022641. IPD Sharing: NO. Countries: 1. Publications: 1.
Assessing Benefits of Near Infrared Autofluorescence (NIRAF) Detection for Identifying Parathyroid Glands During Total Thyroidectomy
ClinicalTrials.gov study NCT05022667. IPD Sharing: NO. Countries: 1. Publications: 1.
Autofluorescence and Indocyanine Green to Avoid Hypocalcemia After Thyroidectomy
ClinicalTrials.gov study NCT05117853. IPD Sharing: YES. Countries: 1. Publications: 22.
Data from: Skin autofluorescence and subclinical atherosclerosis in mild to moderate chronic kidney disease: a case-control study
Advanced glycation end-products (AGEs) are increased and predict mortality in patients with chronic kidney disease (CKD) who are undergoing hemodialysis, irrespective of the presence of type 2 diabetes. However, little information exits about the relationship between AGEs and subclinical atherosclerosis at the early stages of CKD. A case-control study was performed including 87 patients with mild-to-moderate stages of CKD (glomerular filtration rate from 89 to 30 ml/min/per 1.73m2) and 87 non-diabetic non-CKD subjects matched by age, gender, body mass index, and waist circumference. Skin autofluorescence (AF), a non-invasive assessment of AGEs, was measured. The presence of atheromatous disease in carotid and femoral arteries was evaluated using vascular ultrasound, and vascular age and SCORE risk were estimated. Patients with mild-to-moderate stages of CKD showed an increase in skin AF compared with control subjects (2.5±0.6 vs. 2.2±0.4 AU, p<0.001). A skin AF value >2.0 AU was accompanied by a 3-fold increased risk of detecting the presence of an atheromathous plaque (OR 3.0, 95% CI 1.4–6.5, p = 0.006). When vascular age was assessed through skin AF, subjects with CKD were almost 12 years older than control subjects (70.3±25.5 vs. 58.5±20.2 years, p = 0.001). Skin AF was negatively correlated with glomerular filtration rate (r = -0.354, p<0.001) and LDL-cholesterol (r = -0.269, p = 0.001), and positively correlated with age (r = 0.472, p<0.001), pulse pressure (r = 0.238, p = 0.002), and SCORE risk (r = 0.451, p<0.001). A stepwise multivariate regression analysis showed that age and glomerular filtration rate independently predicted skin AF (R2 = 0.289, p<0.001). Skin AF is elevated in patients with mild-to-moderate CKD compared with control subjects. This finding may be independently associated with the glomerular filtration rate and the presence of subclinical atheromatous disease. Therefore, the use of skin AF may help to accurately evaluate the real cardiovascular risk at the early stages of CKD.
FIGURE 1 in Micromorphology of seeds of three Mexican species of Pinguicula (Lentibulariaceae) show autofluorescence using confocal laser scanning microscopy
FIGURE 1. Confocal laser scanning microscopy images showing the micromorphological features of Pinguicula seeds. A–D. Pinguicula casperi; E–H. P. parvifolia; I–L. P. oblongiloba. A, E and I are a general view of the entire seeds (20×, scale bar 100 μm); B, F and J show the micropylar end (60×, scale bar 20 μm); D, H, and L show the chalazal end of the seed (60×, scale bar 20 μm); C, G and K show the surface details of the seeds (100×, scale bar 10 μm).
Example sdt raw FLIM images of NAD(P)H autofluorescence in Drosophila melanogaster tissues
<p>Raw image files of fluorescence lifetime imaging microscopy (FLIM) of NAD(P)H autofluorescence in unstained living <em>Drosophila melanogaster</em> tissues for image analysis. See <a href="https://www.nature.com/articles/s41598-019-56067-w">publication</a> for technical details. Dataset is derived by reduction of original <a href="http://dx.doi.org/10.25532/OPARA-37">dataset</a> to a single channel and selection of individual images. A single 2 channel sdt FLIM image is included also in which the first channel corresponds to the proper NAD(P)H detection range.</p>
Autofluorescence-guided Hemithyroidectomy in a Low-volume, Non-parathyroid Insitution: A Randomized Clinical Trial
ClinicalTrials.gov study NCT05044351. IPD Sharing: NO. Countries: 1. Publications: 1.
Fundus Autofluorescence Imaging in Age-related Macular Degeneration Using Confocal Scanning Laser Ophthalmoscopy
ClinicalTrials.gov study NCT00393692. IPD Sharing: UNDECIDED. Countries: 1. Publications: 25.
Autofluorescence vs Clinical Assessment of Parathyroid Glands During Thyroid Surgery
ClinicalTrials.gov study NCT04509011. IPD Sharing: YES. Countries: 4. Publications: 1.
Near Infrared Autofluorescence Imaging in Minimally-Invasive Video-Assisted Parathyroidectomy (NIRAF MIVAP Study)
ClinicalTrials.gov study NCT06779760. IPD Sharing: NO. Countries: 1. Publications: 1.
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.