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2 results for “bio-oil aqueous phase”
Data for: Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil, Iowa, USA 2023-2025
This dataset contains the experimental design, measurements, and derived variables used to parameterize a techno‑economic model of laccase production via two‑stage solid‑state fermentation of prairie biomass with bio‑oil aqueous phase induction. It includes nutrient screening data for Pleurotus ostreatus growth on prairie biomass with alternative nitrogen sources and a corn‑steep solids dose series; factorial/response‑surface experiments varying substrate bed depth, substrate‑to‑inoculum (S:I) ratio, and pre‑induction growth time; and time‑resolved induction measurements. For each run and replicate, the data record the full set of spectrophotometric absorbances at 0–210 s, fitted slopes and r-square values, dilution and volume factors, and laccase activities normalized per mL and per gram of biomass, alongside the exact culture timings and environmental conditions used in the ABTS assay at 420 nm. Results tables provide the fitted central‑composite design model terms (coefficients, F‑statistics, and p‑values) used directly as inputs to the minimum laccase selling price (MLSP) calculations, together with the underlying per‑condition raw results.
Bio-oil aqueous phase as a laccase inducer in Pleurotus ostreatus: laccase properties and applications, Iowa, USA 2021-2023
This dataset documents the experimental conditions, protocols, and measurements generated during the investigation of bio-oil aqueous phase (AP) as an inducer for laccase enzyme production by Pleurotus ostreatus using prairie biomass as a substrate. The data include detailed descriptions of AP preparation from corn stover pyrolysis, chemical characterization of AP and prairie biomass, and the design of two-stage solid-state fermentation experiments with variables such as AP and copper concentrations, fermentation times, and environmental parameters (pH, temperature). The dataset also contains raw and processed measurements of laccase activity, protein concentration, reducing capacity, and other relevant biochemical properties across multiple experimental runs. In addition to fermentation and enzyme production data, the dataset provides information on downstream application assays. These include quantitative data on dye decolorization (crystal violet, coomassie blue, methyl orange), saccharification yields from prairie biomass pretreated with laccase, and antibiotic detoxification assays using tetracycline.
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