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76 results for “brassinosteroids”
Figure 7 in Mitigation of the effects of salt stress in cowpea bean through the exogenous aplication of brassinosteroid
Figure 7. Effect of 24-epibrasinolide in the activity of the enzyme nitrate reductase of cowpea roots under salt stress. Capital letters indicate statistical differences between EBL treatments (p <0.05) based on upon a Tukey's test; small letters indicate statistical differences between salt treatments (p <0.05) based on upon a Tukey's test.
Figure 6 in Mitigation of the effects of salt stress in cowpea bean through the exogenous aplication of brassinosteroid
Figure 6. Effect of 24-epibrasinolide in the activity of the enzyme nitrate reductase of cowpea leaves under salt stress. Capital letters indicate statistical differences between EBL treatments (p <0.05) based on upon a Tukey's test; small letters indicate statistical differences between salt treatments (p <0.05) based on upon a Tukey's test.
Figure 3 in Mitigation of the effects of salt stress in cowpea bean through the exogenous aplication of brassinosteroid
Figure 3. Effect of 24-epibrasinolide in the stem diameter of cowpea plants under salt stress. Capital letters indicate statistical differences between EBL treatments (p <0.05) based on upon a Tukey's test; small letters indicate statistical differences between salt treatments (p <0.05) based on upon a Tukey's test.
Data from: The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues
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Untargeted mutagenesis of brassinosteroid receptor SbBRI1 confers drought tolerance by altering phenylpropanoid metabolism in Sorghum bicolor
<p>Metabolomics data from the study: Untargeted mutagenesis of brassinosteroid receptor SbBRI1 confers drought tolerance by altering phenylpropanoid metabolism in Sorghum bicolor. Files are raw chromatograms.</p> <p>Metabolite profiling analysis<br>Metabolite Extraction and Quantification. The whole metabolite extraction and quantification pipeline followed the guidelines as described in Giavalisco et al. (2011) and Salem et al. (2020). Briefly, 10-25 mg of fresh plant tissue was harvested and immediately frozen in N2, and ground using zirconia beads with the help of Tissue Lyser Mixer-Mill (Qiagen) 3 min at 25 Hz. After samples were extracted in 100% cold methanol, they were centrifuged for 10 min at 14000 rpm (Room temperature) and 3:1 chloroform was added. After vortexing thoroughly, 1:1 volume of water was added and the samples were subsequently vortexed and centrifuged. The semi-polar phase was used for analysis of semi-polar secondary and primary metabolites. For secondary semi-polar metabolites, the dried polar aliquots were resuspended in water:methanol (1:1 v/v).<br>3 Analysis of semi-polar metabolites was performed using a Thermo Q Exactive Focus coupled to a reverse-phase C18 column. The column was maintained at 40°C with a flow rate of 400 μl/min, and the eluent system consisted of water (eluent A) and acetonitrile (eluent B), both containing 0.1% formic acid. Mass spectra were acquired in full scan mode over a range of 100-1500 m/z using data-independent acquisition (DIA) with high-energy collisional dissociation (HCD) energy set at 30 eV in positive mode.<br>9 For primary metabolite metabolites, the dried polar was derivatized as described in Lisec et al. (2006). Derivatization was carried out at 37°C for 120 min using 40 μl of 20 mg/ml methoxyamine hydrochloride in pyridine, followed by a 30-min treatment at 37°C with 70 μl of trimethylsilyl-N-methyl trifluoroacetamide (MSTFA). The derivatized samples (1 μl) were injected in splitless mode into a gas chromatograph coupled to a time-of-flight mass spectrometer (Pegasus HT TOF-MS). Gas chromatography was performed on a 30-m DB-35 column using helium as the carrier gas. The initial temperature of the oven was 85°C, and it was ramped up at a rate of 15°C/min to a final temperature of 360°C. Mass spectra were recorded in the range of 70-600 m/z at a rate of 20 scans/s. Data Processing and Compound Annotation. LC-MS full scan data were processed using MS Refiner (Expressionist 14.0). Processing of chromatograms, peak detection, and integration were performed using RefinerMS (version 5.3; GeneData). Metabolite identification and annotation were performed using in-house reference compound library, tandem MS (MS/MS) 22 fragmentation, and metabolomics databases (Alseekh et al., 2021). For the annotation of metabolites measured by GC-MS the Golm Metabolome Database was used (Kopka et al., 2005).<br><br></p> <div> <div> <p><span><span>#heatmap of fold changes</span></span><span> </span></p> </div> <div> <p><span><span>ts</span><span> <</span><span>-read.csv(</span><span>"all_log2fc_heat_all.csv", </span><span>sep</span><span>=";", </span><span>fileEncoding</span><span>='latin1</span><span>',check</span><span>.names=F, header=TRUE)</span></span><span> </span></p> </div> <div> <p><span><span>ts1 <- </span><span>ts</span></span><span> </span></p> </div> <div> <p><span><span>ts1$class <- NULL </span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>row.names</span><span>(ts1) <- ts1$compound</span></span><span> </span></p> </div> <div> <p><span><span>ts1$compound <- NULL</span></span><span> </span></p> </div> <div> <p><span><span>m <- </span><span>as.matrix</span><span>(ts1)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>ann</span><span> <- </span><span>ts</span><span>[1:2]</span></span><span> </span></p> </div> <div> <p><span><span>row.names</span><span>(</span><span>ann</span><span>) <- </span><span>ann$compound</span></span><span> </span></p> </div> <div> <p><span><span>ann$compound</span><span> <- NULL</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>install.packages</span><span>("</span><span>pheatmap</span><span>")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>library("</span><span>pheatmap</span><span>")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>m1 <- m[</span><span>rownames</span><span>(</span><span>ann</span><span>)</span><span>, ]</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>pheatmap</span><span>(m1, </span><span>annotation_row</span><span> = </span><span>ann</span><span>, </span><span>cluster_rows</span><span> = </span><span>F,cluster_cols</span><span> = F, </span><span>cellheight</span><span> = 10, </span></span><span> </span></p> </div> <div> <p><span><span> </span><span>cellwidth</span><span> = 10, </span><span>gaps_row</span><span> = </span><span>cumsum</span><span>(</span><span>c(</span><span>3,105,30,11,21,4,32,5,17)), </span><span>gaps_col</span><span> = </span><span>cumsum</span><span>(</span><span>c(</span><span>2,2,2)), scale = 'none')</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>#boxplots</span></span><span> </span></p> </div> <div> <p><span><span>install.packages</span><span>("ggplot2")</span></span><span> </span></p> </div> <div> <p><span><span>install.packages</span><span>("</span><span>readr</span><span>")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>library(ggplot2)</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>readr</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>install.packages</span><span>("</span><span>tidyverse</span><span>")</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>tidyverse</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>grid <- </span><span>read.csv(</span><span>"sec_exp1_logfc_20.csv", </span><span>sep</span><span>=";", header=TRUE)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>grid_g</span><span> <- grid%>% </span><span>gather(</span><span>"compound", "value", 6:25)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>d=</span><span>ggplot</span><span>(data = </span><span>grid_g</span><span>, </span><span>aes</span><span>(x=compound, y=value, fill=factor(group)</span><span>))+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>geom_boxplot</span><span>(</span><span>outlier.shape</span><span> = </span><span>NA)+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>geom_point</span><span>(position = </span><span>position_jitterdodge</span><span>(0.1))</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>d+theme</span><span>(text = </span><span>element_text</span><span>(size = 15</span><span>))+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>scale_fill_manual</span><span>(values = </span><span>c(</span><span>"#ca5826","#e4ab92", "#008080", "#99cccc"</span><span>))+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>labs(</span><span>y="log2</span><span>fc(</span><span>metabolite content)</span><span>",fill</span><span>="</span><span>gtype</span><span>")+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>theme(</span><span>axis.text.x</span><span> = </span><span>element_text</span><span>(angle = 60, </span><span>hjust</span><span> = 1, size = 5)) + </span></span><span> </span></p> </div> <div> <p><span><span> </span><span>theme_classic</span><span>() +</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>coord_flip</span><span>() +</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>facet_grid</span><span>(condition </span><span>~ .</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>ggsave</span><span>("logfcsum_exp1_15_bri1.png", width = 10, height = 10)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>#boxplots all data</span></span><span> </span></p> </div> <div> <p><span><span>library(ggplot2)</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>readr</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>viridis</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>tidyverse</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>dplyr</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sPCA</span><span> <</span><span>-read.csv(</span><span>"GCMS_polar.csv", </span><span>sep</span><span>=",", </span><span>fileEncoding</span><span>='latin1', </span><span>check.names</span><span> = F, header=TRUE)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>row.names</span><span>(</span><span>sPCA</span><span>) <- </span><span>sPCA$Sample</span></span><span> </span></p> </div> <div> <p><span><span>sPCA_D</span><span> <- </span><span>sPCA</span><span>[6:155]</span></span><span> </span></p> </div> <div> <p><span><span>sPCA_Dlog</span><span> <- log(</span><span>sPCA_D</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>sPCA_Dlog10 <- log10(</span><span>sPCA_D</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sPCA_Dlog</span><span> <- </span><span>cbind</span><span>(</span><span>sPCA_Dlog</span><span>, group = </span><span>sPCA$Sample_group</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>sPCA_Dlog</span><span> <- </span><span>sPCA_Dlog</span><span>%>% </span><span>select(</span><span>group, </span><span>everything(</span><span>))</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sPCA_Dlog</span><span> <- </span><span>cbind</span><span>(</span><span>sPCA_Dlog</span><span>, sample = </span><span>sPCA$Sample</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>sPCA_Dlog</span><span> <- </span><span>sPCA_Dlog</span><span>%>% </span><span>select(</span><span>sample, </span><span>everything(</span><span>))</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>write.csv(</span><span>sPCA_Dlog10, "GCMS_polar_lg.csv")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sPCA_Dlog_g</span><span> <- </span><span>sPCA_Dlog</span><span>%>% </span><span>gather(</span><span>"compound", "value", 3:152)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>d <- </span><span>ggplot</span><span>(</span><span>sPCA_Dlog_g</span><span>, </span><span>aes</span><span>(x=sample, y=value, fill=factor(group)</span><span>))+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>geom_boxplot</span><span>(</span><span>outlier.shape</span><span> = </span><span>NA)+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>geom_point</span><span>(position = </span><span>position_jitterdodge</span><span>(0.1))</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>d + </span><span>theme(</span><span>text = </span><span>element_text</span><span>(size = 15</span><span>))+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>scale_fill_manual</span><span>(values = </span><span>c(</span><span>"#CC6677","#882255","#44AA99", "#117733"</span><span>))+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>labs(</span><span>y="</span><span>lg</span><span>(normalized metabolite content)", x= "sample</span><span>" ,</span><span> fill="group</span><span>")+</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>theme(</span><span>axis.text.x</span><span> = </span><span>element_text</span><span>(angle = 60, </span><span>hjust</span><span> = 1)) + </span></span><span> </span></p> </div> <div> <p><span><span> </span><span>theme_classic</span><span>() +</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>geom_hline</span><span>(</span><span>yintercept</span><span>=0, </span><span>linetype</span><span>="dashed", </span><span>color</span><span> = "black")</span></span><span> </span></p> </div> <div> <p><span><span>#PCA</span></span><span> </span></p> </div> <div> <p><span><span>install.packages</span><span>("</span><span>plotly</span><span>")</span></span><span> </span></p> </div> <div> <p><span><span>install.packages</span><span>("</span><span>ggfortify</span><span>")</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>plotly</span><span>)</span></span><span> </span></p> </div> <div> <p><span><span>library(</span><span>ggfortify</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sPCA</span><span> <</span><span>-read.csv(</span><span>"prim.csv", </span><span>sep</span><span>=";", header=TRUE)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sPCA</span><span> <- </span><span>subset(</span><span>sPCA</span><span>, </span><span>experiment!=</span><span>"2")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sPCAtd</span><span> <- </span><span>all_d</span><span>[7:240]</span></span><span> </span></p> </div> <div> <p><span><span>sPCAtdn</span><span> <- </span><span>sPCAtd</span><span>[-</span><span>c(</span><span>24)</span><span>, ]</span></span><span> </span></p> </div> <div> <p><span><span>sPCAn</span><span> <- </span><span>sPCA</span><span>[-</span><span>c(</span><span>24)</span><span>, ]</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>#normal</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sec.pcat</span><span> <- </span><span>prcomp</span><span>(</span><span>sPCAtd</span><span>, </span><span>center</span><span> = </span><span>TRUE,scale</span><span>. = TRUE)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>summary(</span><span>sec.pcat</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>p <- </span><span>autoplot</span><span>(</span><span>sec.pcat</span><span>, data = </span><span>all_d</span><span>, colour = 'condition', shape = 'Genotype') + </span></span><span> </span></p> </div> <div> <p><span><span> </span><span>geom_text</span><span>(</span><span>aes</span><span>(label = Replicate), </span><span>nudge_y</span><span> = 0.02) +</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>theme_classic</span><span>() +</span></span><span> </span></p> </div> <div> <p><span><span> </span></span><span><span>scale_color_manual</span><span>(</span><span>values</span><span>=</span><span>c(</span><span>"#56B4E9", "#E69F00"))</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>ggplotly</span><span>(p)</span></span><span> </span></p> </div> <div> <p><span><span>#</span><span>experiment</span><span> wise fold changes</span></span><span> </span></p> </div> <div> <p><span><span>write.csv(</span><span>sec_fc_mean</span><span>, "sec_foldchange_mean.csv")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1 <- </span><span>sect[</span><span>1]</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$bri1.drought <- </span><span>foldchange(</span><span>sect$X72.bri</span><span>1.drought</span><span>, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$bri1.drought1 <- </span><span>foldchange(</span><span>sect$X72.bri</span><span>1.drought</span><span>.1, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$bri1.drought2 <- </span><span>foldchange(</span><span>sect$X72.bri</span><span>1.drought</span><span>.2, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$bri1.control <- </span><span>foldchange(</span><span>sect$X72.bri</span><span>1.control</span><span>, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$bri1.control1 <- </span><span>foldchange(</span><span>sect$X72.bri</span><span>1.control</span><span>.1, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$bri1.control2 <- </span><span>foldchange(</span><span>sect$X72.bri</span><span>1.control</span><span>.2, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$WT.drought <- </span><span>foldchange(</span><span>sect$X</span><span>72.WT..drought</span><span>, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$WT.drought1 <- </span><span>foldchange(</span><span>sect$X</span><span>72.WT..drought</span><span>.1, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$WT.drought2 <- </span><span>foldchange(</span><span>sect$X</span><span>72.WT..drought</span><span>.2, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$WT.control <- </span><span>foldchange(</span><span>sect$X</span><span>72.WT..control</span><span>, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$WT.control1 <- </span><span>foldchange(</span><span>sect$X</span><span>72.WT..control</span><span>.1, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span><span>sec_fc_exp1$WT.control2 <- </span><span>foldchange(</span><span>sect$X</span><span>72.WT..control</span><span>.2, sect$median.WT.c1)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>write.csv(</span><span>sec_fc_exp1, "sec_fc_exp1.csv")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>#</span><span>all</span><span> compounds for </span><span>RNAsq</span> <span>comarision</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>all_R</span><span> <</span><span>-read.csv(</span><span>"all_RNAsq.csv", </span><span>sep</span><span>=";", </span><span>fileEncoding</span><span>='latin1</span><span>',check</span><span>.names=F, header=T)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>all_fc_exp2 <- </span><span>all_R</span><span>[1]</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>all_fc_exp2$drought <- </span><span>foldchange(</span><span>all_R$bri1_drought, </span><span>all_R$WT_drought</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>all_fc_exp2$control <- </span><span>foldchange(</span><span>all_R$bri1_control, </span><span>all_R$WT_control</span><span>)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>write.csv(</span><span>all_fc_exp2, "all_foldchange_exp2.csv")</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>#students </span><span>t</span><span> test</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>secfc_2_t <</span><span>-read.csv(</span><span>"sec_exp1_logfc.csv", </span><span>sep</span><span>=";", </span><span>fileEncoding</span><span>='latin1</span><span>',check</span><span>.names=F, header=T)</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>secfc_2_t <- secfc_2_</span><span>t[</span><span>-2]</span></span><span> </span></p> </div> <div> <p><span><span>secfc_2_t <- secfc_2_</span><span>t[</span><span>-(14:25)]</span></span><span> </span></p> </div> <div> <p><span> </span></p> </div> <div> <p><span><span>st2<- secfc_2_t %>% </span><span>gather(</span><span>"compound", "value", 6:171)</span></span><span> </span></p> </div> <div> <p><span><span>stat.test1 <- st2 %>%</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>group_by</span><span>(compound) %>%</span></span><span> </span></p> </div> <div> <p><span><span> </span><span>t_test</span><span>(value ~ group)</span></span><span> </span></p> </div> <div> <p><span><span>add_significance</span><span>()</span></span><span> </span></p> </div> <div> <p><span><span>stat.test1</span></span><span> </span></p> </div> </div> <p> </p>
Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root
<p>Supplemental datasets for "Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root". </p>
Data from: The clathrin adaptor complex AP-2 mediates endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis
Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptor-mediated endocytosis.
Raw data for: Phosphate deprivation-induced changes in tomato are mediated by an interaction between brassinosteroid signaling and zinc
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Data from: The clathrin adaptor complex AP-2 mediates endocytosis of BRASSINOSTEROID INSENSITIVE1 in Arabidopsis
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Data from: Brassinosteroids act as a positive regulator of NBR1-dependent selective autophagy in response to chilling stress in tomato
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Transcriptome for: MYB42 inhibits hypocotyl cell elongation by coordinating brassinosteroid homeostasis and signaling in Arabidopsis
<p><span>The precise control of brassinosteroids (BRs) homeostasis and signaling is a prerequisite for hypocotyl cell elongation in plants. Little is known, however, of such regulators of BR homeostasis and signaling. Here, we have demonstrated that MYB42 negatively regulates hypocotyl elongation through partial inhibition of BRASSINAZOLE-RESISTANT 1 (BZR1) signaling and promotion of BR inactivation. Transgenic <i><span>Arabidopsis</span></i> plants repressing the expression of <i><span>MYB42</span></i> and its paralog <i><span>MYB85</span></i> (<i><span>MYB85 RNAi;myb42</span></i>) exhibited longer hypocotyls than wild-type (WT) plants in darkness, while <i><span>MYB42</span></i> or <i><span>MYB85 </span></i>overexpression inhibits hypocotyl elongation. Hypocotyl length of <i><span>MYB85 RNAi;myb42 </span></i><span>plants can</span><i> </i><span>be </span>resorted <span>into WT level by </span><i><span>MYB42 </span></i>overexpression<span>. </span><span>MYB</span><span>42</span> inhibits hypocotyl elongation by mediating BR signaling, because <i><span>MYB42 </span></i>expression is repressed with BR treatment and in the dominant BR mutant <i><span>bzr1-1D</span></i><span>,</span> and <span>mutation of</span><i> </i><span>both</span><i><span> MYB42</span></i><span> and </span><i><span>MYB85 </span></i>enhances the dwarf phenotype of the BR receptor mutant <i><span>bri1-5</span></i><span>. BZR1 directly represses </span><i><span>MYB42 </span></i><span>expression in response to BR, and </span>t<span>he</span><i> </i><span>hypocotyl length of </span><i><span>bzr1-1D</span></i> is reduced in <i><span>MYB42</span></i><span> overexpression plants but increased in</span><i><span> MYB85 RNAi;myb42 </span></i><span>plants. These results show </span><span><span>MYB42 is a negative target of BZR1</span></span><span>. Transcriptome data revealed that</span><span><span> a number of</span></span><span> BZR1-induced </span>genes associated with <span>cell elongation are down-regulated by MYB42, suggesting that </span><span><span>MYB42</span></span> may partially inhibit BZR1 signaling. In addition, MYB42 enlarges its action in BR signaling through feedback activation of <span>the </span>BR-inactivating enzyme<span> DOGT1</span>. The present study shows a MYB42-mediated multilevel system that contributes to fine regulation of BR-induced hypocotyl elongation.</span></p>
RIP2 interacts with REL1 to control leaf architecture by modulating brassinosteroid signaling in rice
<p>FPKM (Fragments per kilobase of exon model per million mapped fragments) value of all detected genes in in WT, rel1, rip2, and rel1 rip2 mutants. ZH11_1, ZH11_2, ZH11_3 represent samples were collected from ZH11 with three independent biological replicates. rel1_1, rel1_2, rel1_3 represent samples were collected from <i>rel1 </i>mutants with three independent biological replicates. rip2_1, rip2_2, rip2_3 represent samples were collected from rip2 mutant with three independent biological replicates. rel1rip2_1, rel1rip2_2, rel1rip2_3<b> </b>represent samples were collected from rel1rip2 double mutant with three independent biological replicates.</p>
Figure 2 in Mitigation of the effects of salt stress in cowpea bean through the exogenous aplication of brassinosteroid
Figure 2. Effect of 24-epibrasinolide in the number of leaves of cowpea plants under salt stress. Capital letters indicate statistical differences between EBL treatments (p <0.05) based on upon a Tukey's test; small letters indicate statistical differences between salt treatments (p <0.05) based on upon a Tukey's test.
Figure 1 in Mitigation of the effects of salt stress in cowpea bean through the exogenous aplication of brassinosteroid
Figure 1. Effect of 24-epibrasinolide in the height of cowpea plants under salt stress. Capital letters indicate statistical differences between EBL treatments (p <0.05) based on upon a Tukey's test; small letters indicate statistical differences between salt treatments (p <0.05) based on upon a Tukey's test.
Transcriptome for: MYB42 inhibits hypocotyl cell elongation by coordinating brassinosteroid homeostasis and signaling in Arabidopsis
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RIP2 interacts with REL1 to control leaf architecture by modulating brassinosteroid signaling in rice
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Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses [RNA-seq]
GEO Series GSE37159. Arabidopsis thaliana. 8 samples. Type: Expression profiling by high throughput sequencing.
A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root
GEO Series GSE181178. Arabidopsis thaliana. 2 samples. Type: Expression profiling by high throughput sequencing.
The AP2/ERF Transcription Factor TINY Modulates Brassinosteroid-Regulated Plant Growth and Drought Response in Arabidopsis
GEO Series GSE128946. Arabidopsis thaliana. 9 samples. Type: Expression profiling by high throughput sequencing.
Brassinosteroid (BR) plant hormone signaling
GEO Series GSE25135. Arabidopsis thaliana. 10 samples. Type: Genome binding/occupancy profiling by genome tiling array; Expression profiling by array.
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