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18,200 results for “breast”
Phindr3D: Test Data Set 2 (human MCF10A breast cancer organoids)
<p>3D confocal image stacks of human MCF10A breast cancer organoids expressing different oncogenes to test the functionality of Phindr3D. Explanatory .txt file contained in the ZIP files.</p> <p>Please see the manuscript for details and on how to access the full data set:</p> <p> </p> <p><strong>Rapid 3D phenotypic analysis of neurons and organoids using data-driven cell segmentation-free machine learning</strong></p> <p>Philipp Mergenthaler*, Santosh Hariharan*, James M. Pemberton, Corey Lourenco, Linda Z. Penn, David W. Andrews</p> <p><em>PLOS Computational Biology, DOI: <a href="https://dx.doi.org/10.1371/journal.pcbi.1008630">10.1371/journal.pcbi.1008630</a></em></p> <p> </p> <p><strong>Phindr3D is available on GitHub</strong>: <a href="https://github.com/DWALab/Phindr3D">GitHub - DWALab/Phindr3D</a></p>
Primary Breast Tumor Atlas
<p>Integrated scRNA-seq atlas of primary breast tumors containing 236,363 cells from 119 biopsy samples across eight original source datasets. <a title="Persistent link using digital object identifier" href="https://doi-org.foyer.swmed.edu/10.1016/j.xcrm.2024.101511" target="_blank" rel="noreferrer noopener"><span>https://doi.org/10.1016/j.xcrm.2024.101511</span></a></p>
Inter-Chemical Correlation results for the study: HHEARx2017-1977 (Microbial and metabolic variations mediate the influence of childhood and adolescent EDC and trace element exposure on breast density.)
Title: Microbial and metabolic variations mediate the influence of childhood and adolescent EDC and trace element exposure on breast density. <br>Species: Homo sapiens <br>Number of samples: 1116 <br>Number of named analytes: 41 <br>Datasource url: https://hheardatacenter.mssm.edu/PublicFile/ViewPublicFile?projectid=46 <br>
Infrared Chemical Image of a Breast Cancer Tissue Microarray
<p>This data set relates to an open access paper published in Analyst <em>Exploring AdaBoost and Random Forests machine learning approaches for infrared pathology on unbalanced data sets</em> by Jiayi Tang, Alex Henderson* and Peter Gardner. <a href="https://doi.org/10.1039/D0AN02155E"> https://doi.org/10.1039/D0AN02155E</a></p> <p>The files in this archive are mid-infrared spectroscopy chemical images of a breast cancer tissue microarray. The tissue microarray is BR20832 from Biomax. <a href="http://www.biomax.us/tissue-arrays/Breast/BR20832">http://www.biomax.us/tissue-arrays/Breast/BR20832</a></p> <p>Processed versions of these data in MATLAB file format can be found in another Zenodo archive at <a href="https://doi.org/10.5281/zenodo.4730312">https://doi.org/10.5281/zenodo.4730312</a></p> <p>This processed data refers to a paper published in Analyst</p>
Pre-training with simulated ultrasound images for breast mass segmentation and classification - dataset
<p>Dataset assosiated with the MICCAI Workshop on Data Engineering in Medical Imaging paper: "Pre-training with Simulated Ultrasound Images for Breast Mass Segmentation and Classification"</p>
Kuskokwim River Floodplain: White Spruce (Picea glauca) annual tree-ring width measurements (mm) at breast height from tree-core samples taken above Red Devil on the Kuskokwim River in July, 2007
This dataset contains annual raw ring width measurements in the Tucsan (decadal format) (.rwl file extension) of 14 large white spruce trees growing within 50m of the Kuskokwim River. Ring-widths were measured to 0.001mm on a velmex laser micrometer and accuracy was checked by crossdating using COFECHA. Annual values were measured from 1779-2006.
Canopy Trimming Experiment (CTE) plants greater than 1 centimeter diameter at breast height (DBH)
Plant diameters and growth measurements were taken both before and after treatments to determine how alterations in canopy openness and detritus affect measures of growth, mortality, and production. Both types of trimmed plots are predicted to have relatively quick evidence of re-sprouting (1-3 months; based on Hurricane Hugo studies), especially palms. Because essentially complete defoliation to the canopy took place in the trim plots, the large stems that were trimmed should experience delayed growth and higher mortality than those in non-trimmed plots. The understory vegetation, or otherwise those that were not trimmed (i.e., stems <10cm at dbh) should experience increased growth in both trim plots as a result of increased light. Some nutrient immobilization may occur in the detritus addition plots, but this is predicted to affect belowground processes most. Little influence on aboveground processes from detritus addition might occur and cause some stunted growth rates of stems. Support for this work was provided by grants BSR-8811902, DEB-9411973, DEB-9705814 , DEB-0080538, DEB-0218039 , DEB-0620910 , DEB-1239764, DEB-1546686, and DEB-1831952 from the National Science Foundation to the University of Puerto Rico as part of the Luquillo Long-Term Ecological Research Program. Additional support provided by the University of Puerto Rico and the International Institute of Tropical Forestry, USDA Forest Service.
Tumor growth kinetics of human LM2-4LUC+ triple negative breast carcinoma cells
<p><strong>Cell culture and data set</strong></p> <p>Tumor growth data used in this study were obtained from experiments involving the use of a LM2-4<sup>LUC+</sup> cells (or LM2-4), a metastatic variant of the human triple-negative breast carcinoma MDA-MB-231 cells. Animal studies were performed as described previously under Roswell Park Comprehensive Cancer Center (RPCCC) Institutional Animal Care and Use Committee (IACUC) protocol number 1227M [1-7]. Tumor growth data were pooled from eight separate experiments conducted with a total of 581 observations, and represent control (vehicle-treated) animals from published studies [1-7]. Vehicle formulation was carboxymethylcellulose sodium (USP, 0.5% w/v), NaCl (USP, 1.8% w/v), Tween-80 (NF, 0.4% w/v), benzyl alcohol (NF, 0.9% w/v), and reverse osmosis deionized water (added to final volume) and adjusted to pH 6 (see [3]) and was given at 10ml/kg/day for 7-14 days prior after tumor implantation and before tumor resection [1-7].</p> <ul> </ul> <p><strong>Tumor injections</strong></p> <p>LM2-4<sup>LUC+</sup> cells were orthotopically implanted (10<sup>6</sup> cells per injection) into the right inguinal mammary fat pads of 6- to 8-week-old female severe combined immunodeficient (SCID) mice.</p> <p><strong>Tumor measurements</strong></p> <p>Tumor size was measured regularly with calipers to a maximum volume of 2 cm<sup>3</sup>, calculated by the formula </p> <p><span class="math-tex">\(V = \frac{\pi}{6} w^2 L\)</span></p> <p>(ellipsoid) where <em>L</em> is the largest and <em>w</em> is the smallest tumor diameter.</p> <p><strong>Please cite: </strong>Vaghi C, Rodallec A, Fanciullino R, Ciccolini J, Mochel JP, et al. (2020) Population modeling of tumor growth curves and the reduced Gompertz model improve prediction of the age of experimental tumors, PLoS Comput Biol, 16, p. e1007178. <a href="https://doi.org/10.1371/journal.pcbi.1007178">https://doi.org/10.1371/journal.pcbi.1007178</a></p> <p> </p> <p>In the file, the columns correspond to:</p> <ul> <li>ID: identifier of the animal</li> <li>Time: day of the tumor measurement after implantation</li> <li>Observation: tumor measurement (in mm<sup>3</sup>)</li> </ul> <p> </p> <p><strong>References</strong></p> <p>[1] Benzekry, S., Lamont, C., Beheshti, A., Tracz, A., Ebos, J. M. L., Hlatky, L., & Hahnfeldt, P. (2014). Classical mathematical models for description and prediction of experimental tumor growth. PLoS Comput Biol, <em>10</em>(8), e1003800. http://doi.org/10.1371/journal.pcbi.1003800</p> <p>[2] Benzekry S, Tracz A, Mastri M, Corbelli R, Barbolosi D, Ebos JML. (2016) Modeling Spontaneous Metastasis Following Surgery: An In Vivo-In Silico Approach. Cancer Res.;76(3):535–547. doi:10.1158/0008-5472.CAN-15-1389.</p> <p>[3] Ebos JML, Lee CR, Bogdanovic E, Alami J, Van Slyke P, Francia G, et al. (2008) Vascular Endothelial Growth Factor-Mediated Decrease in Plasma Soluble Vascular Endothelial Growth Factor Receptor-2 Levels as a Surrogate Biomarker for Tumor Growth. Cancer Res.;68(2):521–529. doi:10.1158/0008-5472.CAN-07-3217.</p> <p>[4] Ebos JML, Mastri M, Lee CR, Tracz A, Hudson JM, Attwood K, et al. (2014) Neoadjuvant antiangiogenic therapy reveals contrasts in primary and metastatic tumor efficacy. EMBO Mol Med;6:1561–76. https://doi.org/10.15252/emmm.201403989</p> <p>[5] Ebos JML, Lee CR, Cruz-Munoz W, Bjarnason GA, Christensen JG, Kerbel RS. (2009) Accelerated metastasis after short-term treatment with a potent inhibitor of tumor angiogenesis. Cancer Cell;15:232–9. https://doi.org/10.1016/j.ccr.2009.01.021</p> <p>[6] Mastri M, Tracz A, Lee CR, Dolan M, Attwood K, Christensen JG, et al. (2018) A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal. Cell Rep.; 25 (13):3706–20 e8. Epub 2018/12/28. https://doi.org/10.1016/j.celrep.2018.12.017</p> <p>[7] Vaghi C, Rodallec A, Fanciullino R, Ciccolini J, Mochel JP, et al. (2020) Population modeling of tumor growth curves and the reduced Gompertz model improve prediction of the age of experimental tumors, PLoS Comput Biol, 16, p. e1007178. <a href="https://doi.org/10.1371/journal.pcbi.1007178">https://doi.org/10.1371/journal.pcbi.1007178</a></p>
Detection of HER2+ Breast Cancer Cells using Bioinspired DNA-Based Signal Amplification
<p>Circulating tumor cells (CTC) are promising biomarkers for metastatic cancer detection and monitoring progression. However, CTC detection remains challenging due to their low frequency and heterogeneity. Herein, we report a bioinspired approach to detect individual cancer cells, based on a signal amplification cascade using a programmable DNA hybridization chain reaction (HCR) circuits. We applied this approach to detect HER2+ cancer cells using the anti-HER2 antibody (trastuzumab) coupled to initiator DNA eliciting a HCR cascade that leads to a fluorescent signal at the cell surface. At 4°C, this HCR detection scheme resulted in highly efficient, specific and sensitive signal amplification of the DNA hairpins specifically on the membrane of the HER2+ cells in a background of HER2- cells and peripheral blood leukocytes, which remained almost non-fluorescent. The results indicate that this system offers a new strategy that may be further developed toward an in vitro diagnostic platform for the sensitive and efficient detection of CTC.</p>
Raw read counts and phased SNP counts for every single cell in the sequencing datasets of the breast cancer patient S1 from "Characterizing allele- and haplotype-specific copy numbers in single cells with CHISEL"
<p>This dataset contains the raw read counts and phased SNP counts for every single cell in the sequencing datasets of breast cancer patient S1 from “Characterizing allele- and haplotype-specific copy numbers in single cells with CHISEL” [Zaccaria & Raphael, 2020]. These data enable the full reproduction of all the results in the related manuscript for breast cancer patient S1. Specifically, the data are provided in two files for every dataset <em>DAT</em> of patient S1 with the following format:</p> <ol> <li><em>DAT.raw</em>_<em>read</em>_<em>counts.bed.gz </em>is a multi-cell BED file containing the raw read counts in the following fields: <ul> <li>CHROMOSOME: the name of a human chromosome</li> <li>START: the starting genomic position of a genomic bin in the chromosome</li> <li>END: the ending genomic position of the genomic bin in the chromosome</li> <li>CELL: the cell barcode that uniquely identifies a cell</li> <li>NORMAL: the raw read count for the specified bin from a matched-normal sample</li> <li>COUNT: the raw read count for the specified bin in the specified cell</li> <li>RDR: the estimated read-depth ratio for the specified bin in the specified cell</li> </ul> </li> <li><em>DAT.phased</em>_<em>snps</em>_<em>counts.pos.gz </em>is a multi-cell POS file containing the phased SNP counts in the following fields: <ul> <li>CHROMOSOME: the name of a human chromosome</li> <li>POS: the genomic position in the chromosome of a germline SNP</li> <li>CELL: the cell barcode that uniquely identifies a cell</li> <li>COUNT_HAPLOTYPE_A: the count of reads that cover the SNP and that belong to haplotype A in the specified cell</li> <li>COUNT_HAPLOTYPE_B: the count of reads that cover the SNP and that belong to haplotype B in the specified cell</li> </ul> </li> </ol> <p>All the files have been compressed using standard <em>gzip</em>.</p>
Raw read counts and phased SNP counts for every single cell in the sequencing datasets of the breast cancer patient S0 from "Characterizing allele- and haplotype-specific copy numbers in single cells with CHISEL"
<p>This dataset contains the raw read counts and phased SNP counts for every single cell in the sequencing datasets of breast cancer patient S0 from “Characterizing allele- and haplotype-specific copy numbers in single cells with CHISEL” [Zaccaria & Raphael, 2020]. These data enable the full reproduction of all the results in the related manuscript for breast cancer patient S0. Specifically, the data are provided in two files for every dataset <em>DAT</em> of patient S0 with the following format:</p> <ol> <li><em>DAT.raw</em>_<em>read</em>_<em>counts.bed.gz </em>is a multi-cell BED file containing the raw read counts in the following fields: <ul> <li>CHROMOSOME: the name of a human chromosome</li> <li>START: the starting genomic position of a genomic bin in the chromosome</li> <li>END: the ending genomic position of the genomic bin in the chromosome</li> <li>CELL: the cell barcode that uniquely identifies a cell</li> <li>NORMAL: the raw read count for the specified bin from a matched-normal sample</li> <li>COUNT: the raw read count for the specified bin in the specified cell</li> <li>RDR: the estimated read-depth ratio for the specified bin in the specified cell</li> </ul> </li> <li><em>DAT.phased</em>_<em>snps</em>_<em>counts.pos.gz </em>is a multi-cell POS file containing the phased SNP counts in the following fields: <ul> <li>CHROMOSOME: the name of a human chromosome</li> <li>POS: the genomic position in the chromosome of a germline SNP</li> <li>CELL: the cell barcode that uniquely identifies a cell</li> <li>COUNT_HAPLOTYPE_A: the count of reads that cover the SNP and that belong to haplotype A in the specified cell</li> <li>COUNT_HAPLOTYPE_B: the count of reads that cover the SNP and that belong to haplotype B in the specified cell</li> </ul> </li> </ol> <p>All the files have been compressed using standard <em>gzip</em>.</p>
2 million histological images of breast cancer tumors with her2 labels
<p><strong>Data Description</strong><br> This is a 2 million set of non-overlapping image patches from hematoxylin & eosin (H&E) stained histological images of human breast cancer tumor tissue.</p> <p>The anonymized dataset comes from a cohort of BC patients from the A. C. Camargo Cancer Center (ACCCC, N = 504). All patients were treated for breast cancer at the ACCCC between 2019 and 2021. As part of their diagnosis, in HER2 IHC score 2+ cases, patients' HER2 status was determined following the ASCO guidelines updated in 2018, with visual evaluation of IHC assay and either a FISH or DDISH test. All cases with metastasis or neoadjuvant treatment were excluded.</p> <p>A total of 426 H&E stained high resolution images (40x magnification) were scanned from biopsy and resection tissue samples with a Leica Aperio AT2 scanner. Ethical approval of the ACCCC study was given by the ethics committee of the Fundação Antônio Prudente. We divided the cases into the following 3 groups according to the results of the IHC and ISH tests: HER2-negative, HER2-low and HER2-high.</p> <p>The slides were divided into 256 px x 256 px tiles at 0.5 um/pixel magnification. Then, we used a custom trained ConvNext-tiny neural network to only include tiles from the tumor region and its environment, generating a total of 2051877 image patches.</p> <p>A sample is considered her2-negative with an IHC score of 0; her2-low with an IHC score of 1+ or an IHC score of 2+ with a negative ISH-based test result, and her2-high with an IHC score of 2+ with a positive ISH-based test or an IHC score of 3+.</p> <p>The accompanying code used for training the models is available at https://github.com/tojallab/wsi-mil</p>
Sociocultural correlates of exclusive breast feeding among postpartum mothers in Kwara state
<p>The data is about the determinants of exclusive breast feeding among postpartum mothers in Kwara state, Nigeria</p>
Breast Micro-Calcifications Dataset with Precisely Annotated Sequential Mammograms
<p><strong>Dataset Version 3 Update</strong></p> <p><strong>The ground truth images (.jpg) match the dimensions of the corresponding original images (.dcm), ensuring consistency across the dataset.</strong><br><br></p> <p><strong>Breast Micro-Calcifications Dataset with Precisely Annotated Sequential Mammograms</strong></p> <p><strong>Citing the Dataset</strong></p> <p>The dataset is released under a Creative Commons Attribution license, so please cite the dataset if it is used in your work in any form. Published academic papers should use the academic paper citation for our paper. Personal works, such as projects or blog posts, should provide a URL to this Zenodo page, though a reference to our paper would also be appreciated.</p> <p><em>Academic paper citation</em></p> <p>Loizidou, K., Skouroumouni, G., Pitris, C. <em>et al.</em> Digital subtraction of temporally sequential mammograms for improved detection and classification of microcalcifications. <em>Eur Radiol Exp</em> <strong>5, </strong>40 (2021). https://doi.org/10.1186/s41747-021-00238-w</p> <p><em>Personal use citation</em></p> <p>Include a link to this Zenodo page - 10.5281/zenodo.14859694</p> <p><strong>ACKNOWLEDGMENT</strong></p> <p>This research is funded by the European Union’s Horizon 2020 research and innovation program under grant agreement No. 739551 (KIOS CoE) and from the Republic of Cyprus through the Directorate General for European Programs, Coordination and Development.</p> <p><strong>Contact Information</strong></p> <p>If you would like further information about the dataset, or if you experience any issues downloading files, please contact us at cloizi01@ucy.ac.cy.</p> <p><strong>General Information</strong></p> <p>This dataset consists of 100 pairs of mammograms, from two temporally sequential rounds. Specifically, this dataset includes the prior and recent mammograms of CC and MLO view of each patient. This is a complete dataset for the detection and BI-RADS classification of breast micro-calcifications, using digital mammograms. It contains normal (BI-RADS 1), benign (BI-RADS 2), and suspicious (BI-RADS 4-5) cases, and for each mammogram, an image with precise annotation of each individual micro-calcification, by two expert radiologists, is provided. In 32 suspicious cases, the biopsy results are also available.</p> <p><strong>More details are available in the README.txt</strong></p>
Multi-omic machine learning predictor of breast cancer therapy response
<p>H&E slides used in the training dataset described in "Multi-omic machine learning predictor of breast cancer therapy response" published in <em>Nature</em>: <a href="https://www.nature.com/articles/s41586-021-04278-5">https://www.nature.com/articles/s41586-021-04278-5</a></p> <p>Metadata associated with these images also included in file Slide metadata.xlsx</p>
Breast Masses Dataset with Precisely Annotated Sequential Mammograms
<p><strong>BREAST MASSES DATASET WITH PRECISELY ANNOTATED SEQUENTIAL MAMMOGRAMS</strong></p> <p><strong>Citing the Dataset</strong></p> <p>The dataset is released under a Creative Commons Attribution license, so it is mandatory to cite the dataset if you use it in your work in any form. Published academic papers should use the academic paper citation of our paper. Personal works, such as projects or blog posts, should provide a URL to this Zenodo page, though a reference to our paper would also be appreciated.</p> <p><em>Academic paper citation</em></p> <p>TBA</p> <p><em>Personal use citation</em></p> <p>Include a link to this Zenodo page - 10.5281/zenodo.11446259</p> <p><strong>ACKNOWLEDGMENT</strong></p> <p>The publication of this paper is supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No 739551 (KIOS CoE) and the Government of the Republic of Cyprus through the Cyprus Deputy Ministry of Research, Innovation and Digital Policy.</p> <p><strong>Contact Information</strong></p> <p>If you would like further information about the dataset, or if you experience any issues downloading files, please contact us at cloizi01@ucy.ac.cy.</p> <p><strong>General Information</strong></p> <p>This dataset consists of 100 pairs of mammograms, from two temporally sequential rounds. Specifically, this dataset includes the prior and recent mammograms with two mammographic views for each patient. This is a complete dataset for the detection and classification of breast masses, using sequential mammograms. It contains normal (BI-RADS 1), benign (BI-RADS 2), and biopsy-confirmed malignant cases (BI-RADS 6). For each mammogram, an image with precise annotation of each individual mass, by two expert radiologists, is provided.</p> <p><strong>More details are available in the README.txt</strong></p>
Evaluation of transcription factor knockout impact on paclitaxel response for Triple Negative Breast Cancer
<div>Data and code related to Zenodo repository: 10.5281/zenodo.11238552</div> <div> </div> <div>Two experimental formats included:</div> <div>'fixed' prefix: data from terminal time point of siRNA screen applied to HCC1143, HCC1806, and MDA-MB-468 Triple Negative Breast Cancer cell lines.</div> <div>'live' prefix: data from live-cell imaging of cell cycle reporter (HDHB-mClover/NLS-mCherry) HCC1143 Triple Negative Breast Cancer cell line.</div> <div>Note: 'live' level 1 data is available upon request (heiserl@ohsu.edu, calistri@ohsu.edu).</div> <div> </div> <div>Experimental goal:</div> <div>Evaluate whether siRNA knockdown of transcription factors elevated during paclitaxel response impact cell count, cell morphology or cycling dynamics.</div> <div> </div> <div>Methods:</div> <div>siRNA Knockdown: Cells were plated in 90ul of serum free media per well of a 96 well plate. 24 hours later, siRNA knockdown mixture was prepared using a cell-line optimized concentration of Lipofectamine RNAiMAX (cat 13778075-075, Invitrogen) and siRNA (Horizon Discovery ON-TARGETplus) following RNAiMAX recommended protocol. The final concentration of siRNA per well was 1pmol and the final volume of RNAiMAX per well was 75nL for HCC1143, and 37.5nL for HCC1806 or MDA-MB-468 in 100uL of cell containing volume. 24 hours after siRNA transfection cells were treated with an addition of 100uL complete media containing either DMSO vehicle control or paclitaxel. </div> <div> </div> <div>Fixed-cell assays: Cells were plated at 3000 cells in 100ul of complete media per well in a 96 well plate (#08-772-225, FisherScientific). After 24 hours, an additional 100ul of either vehicle (0.1% DMSO) or paclitaxel containing complete media was added. After 72 hours cells were fixed with 4% Formaldehyde (#28908, ThermoFisher Scientific) for 15 minutes at room temperature, then permeabilized with 0.3% Triton X-100 (#X100-100ML, Sigma Aldrich) for 10 minutes at room temperature, then washed twice with PBS. Fixed cells were then stained with 0.5ug/mL DAPI (4083S, Cell Signaling Technology) in PBS for 15 minutes at room temperature. Following DAPI staining, wells were washed once with PBS, then stained with 1:20,000 HCS CellMask Green in PBS (H32714, Invitrogen) for 15 minutes at room temperature. Wells were washed twice with room temperature PBS and then 4 fields of view per well imaged on an InCell 6000 (GE Healthcare). Images were segmented with two custom Cellpose models to segment the nucleus (using parameters: diameter = 50, chan = DAPI, chan2 = Cellmask Orange) and cytoplasm (using parameters: diameter = 90, chan = Cellmask Orange, chan2 = DAPI). Image quantification was performed in R (v4.3.1) using EBImage (v4.42.0), and cells were annotated based on the number of distinct nuclei segmented within each cytoplasmic mask. </div> <div> </div> <div>HDHB reporter live-cell assays: siRNA knockdown and drug treatment was performed as described above, and then the plate was loaded on an Incucyte S3 (Sartorious) and cells imaged every 15 minutes for 72 hours post drug treatment. At each timepoint 4 fields of view were captured at 20x magnificantion in each well using the phase, red and green channels. A cytoplasmic mask was computed from the mean of normalized red/green channel (cellpose parameters: diameter = 57, chan = mean(normalized(red), normalized(green)), and a nuclear mask was computed from the red channel (cellpose parameters: diameter = 30, chan = DAPI) using custom trained Cellpose models. Image quantification was performed in R (v4.3.1) using EBImage (v4.42.0). An additional perinuclear ring mask was computed as the 11 pixel dilation from the nuclear mask, but still bound by the cytoplasmic mask. To determine mClover localization thresholds for cell cycle assignment, 250 cell images were randomly selected and manually assigned to the G1, S/G2 or M cell cycle state based on mClover localization. The mClover intensity ratios were then used to determine thresholds for automated cell cycle phase calling which was applied to the rest of the data set (Supplemental Figure 5A). Mononuclear cells with a Perinuclear:Nuclear mean intensity ratio greater than 0.8 and Nuclear:Cytoplasmic total intensity less than 0.5 were assigned to the S/G2 phase. Mononuclear and Multinuclear cells with a Nuclear:Cytoplasmic total intensity ratio greater than 0.8 and Perinuclear:Nuclear mean intensity ratio less than 0.8 were assigned to the ‘M’ phase. The remainder of mononuclear cells were assigned ‘G1’, and the remainder of multinucleated cells were assigned ‘Multinucleated’. </div> <div> </div> <div>Included files:</div> <div>fixed_level_1-plate_#.zip : Six .zip archives containing the raw images (DAPI/CellMask/Brightfield) from fixed-cell experiments.</div> <div>plate 1: HCC1143 cells treated with plate A schema</div> <div>plate 2: HCC1143 cells treated with plate B schema</div> <div>plate 3: HCC1806 cells treated with plate A schema</div> <div>plate 4: HCC1806 cells treated with plate B schema</div> <div>plate 5: MDA-MB-468 cells treated with plate A schema</div> <div>plate 6: MDA-MB-468 cells treated with plate B schema</div> <div>fixed_level_2: Data quantified from cellpose masks at the single-nuclei level (redundant cytoplasm information)</div> <div>fixed_level_3: Data from 'fixed_level_2.csv' collapsed to the single cell level, including staining intensity and aggregate nuclear information</div> <div>fixed_incell_to_cellpose.rmd: R markdown code for converting original incell files (fixed_level_1) to RGB images for cellpose segmentation</div> <div>fixed_image_quantification.rmd: R markdown code for quantifying images using cellpose segmentation masks and original images (fixed_level_1)</div> <div>fixed_cellpose_models.zip: Archive including cellpose models used for fixed experiment</div> <div>live_level_2: Data quantified from cellpose masks at the single-nuclei level (redundant cytoplasm information)</div> <div>live_level_3: Data from 'live_level_2.csv' collapsed to the single cell level, including staining intensity and aggregate nuclear information</div> <div>live_level_4: Data from 'live_level_3.csv' collapsed to the single condition level summarizing the number, multinucleation status and phase of cells at each time point.</div> <div>live_image_quantification.rmd: R markdown code for quantifying images using cellpose segmentation masks and original images (live_level_1).</div> <div>l ive_incu_archive2rgb.rmd: R markdown code for converting incucyte archive formatted data into RGB images, where the blue channel is the arithmetic mean of the min-max (0-1) normalized red and green channels.</div> <div>live_cellpose_models.zip: Archive including cellpose models used for live experiment.</div> <div> </div> <div> </div>
Impact of paclitaxel treatment on the Triple Negative Breast Cancer Cell line HCC1143
<div>Data and code related to Zenodo repository: 10.5281/zenodo.11237850</div> <div> </div> <div>Experimental goal:</div> <div>Evaluate the impact of escalating paclitaxel dose on cell count, nuclear morphology and cellular outcome.</div> <div> </div> <div>Methods:</div> <div>Cells were plated at 3000 cells in 100ul of complete media per well in a 96 well plate (#08-772-225, FisherScientific). After 24 hours, an additional 100ul of either vehicle (0.1% DMSO) or paclitaxel containing complete media was added. After 72 hours cells were fixed with 4% Formaldehyde (#28908, ThermoFisher Scientific) for 15 minutes at room temperature, then permeabilized with 0.3% Triton X-100 (#X100-100ML, Sigma Aldrich) for 10 minutes at room temperature, then washed twice with PBS. Fixed cells were blocked with 1% BSA (A7906-100G, Millipore Sigma) in PBS for 1 hour at room temperature and then stained overnight with 1:100 anti-CDKN2A/p16INK4A+CDKN2B/p15INK4B-AF644 (#ab199756, Abcam), and 1:100 anti-cPARP-AF647 (#6987S, Cell Signaling Technology) or 1:500 anti-TUBB3-AF647 (#ab190575, Abcam) overnight at 4C. Each well was washed twice with room temp PBS then stained with 0.5ug/mL DAPI (4083S, Cell Signaling Technology) in PBS for 15 minutes at room temperature. Following DAPI staining, wells were washed once with PBS, then stained with 1:20,000 HCS CellMask in PBS (Orange: #H32713, Green: #H32714, Invitrogen) for 15 minutes at room temperature. Wells were washed twice with room temperature PBS and then 4 fields of view per well imaged on an InCell 6000 (GE Healthcare). Images were segmented with two custom Cellpose models to segment the nucleus (using parameters: diameter = 45, chan = DAPI, chan2 = Cellmask Orange) and cytoplasm (using parameters: diameter = 90, chan = Cellmask Orange, chan2 = DAPI). Image quantification was performed in R (v4.3.1) using EBImage (v4.42.0), and cells were annotated based on the number of distinct nuclei segmented within each cytoplasmic mask. </div> <div> </div> <div>Included files:</div> <div>row_#_level_1.zip : 6 zip file containing original images from InCell 6000, one zip per row</div> <div>level_2.csv : Data quantified to the nuclear level (cytoplasmic quantification is duplicates across multiplet nuclei)</div> <div>level_3.csv: Data quantified at the cellular level including number of nuclei and stain intensities for segmented compartments</div> <div>platemap.csv: Description of each well from the stained plate</div> <div>cellpose_modelz.zip: Zip file containing the two CellPose models used for segmentation</div> <div>image_quantification.rmd : R markdown file containing code for extracting and quantifying image intensities using the raw images (level_1) and segmentation masks created from cellpose.</div>
Quality of Life of Breast Cancer Patients in Albania
<p>Quality of Life of Breast Cancer Patients in Albania. <span>For this study, the European Organization for Research and Treatment of Cancer Quality of Life (EORTC QLQ – C30) questionnaire was used</span></p>
Single-cell RNA-seq of breast cancer infiltrating T cells (case 1)
<p>Single cell suspensions were generated from two individual TNBC primary tumor samples (this entry contains case 2) and the viable cells were FACS sorted for CD3<sup>+</sup> T cells. Sorted cells were then counted and assessed for viability. Single cell library preparation was carried out as per the 10X Genomics Chromium Single cell protocol for the v2 reagent kit (10X Genomics, Pleasanton, CA, USA). Cell suspensions were loaded onto a Chromium Single Cell Chip along with the reverse transcription (RT) mastermix and single cell 3’ gel beads. Following generation of single cell gel bead-in-emulsions (GEMs), reverse transcription was performed using a C1000 Touch Thermal Cycler with a Deep Well Reaction Module (Bio-Rad Laboratories, Hercules, CA, USA). Amplified cDNA was purified using SPRIselect beads (Beckman Coulter, Lane Cove, NSW, Australia) and sheared to approximately 200bp with a Covaris S2 instrument (Covaris, Woburn, MA, USA) using the manufacturer’s recommended parameters. Sequencing libraries were generated with unique sample indices (SI) for each sample. Libraries were sequenced on an Illumina HiSeq 2500 High Output Mode using V4 clustering and sequencing chemistry.</p> <p>This dataset contains the raw .bcl files.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.