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ShareScore release 0.9.0
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210 results for “c2c12”
C2C12 and THP1 images Brightfield and Hoechst
<p>20221207_1700_gt_C2C12 has 2x 96 TIF files with Brightfield and Hoechst stained images of C2C12 cells plated in 96 well plates the day before imaging</p> <p>20231106_1300_mm_THP1 has 2x 96 TIF files with Brightfield and Hoechst stained images of THP1 cells cultured in 96 well plates for two days in 100ng/ml PMA, and 1 day in routine culture meidum before imaging.</p> <p><span>Wells of the 96-well plates were imaged automatically using a Cytation-1 Cell imaging multimode reader (Agilent) set at 37 degrees Celsius using the Gen5 (version 3.12) software. For brightfield images, one image per well was captured with an Olympus 4x UPLFLN objective, using user-trained auto-focus and manual exposure settings that were set before imaging of each plate. For nuclei counting, 4 </span><span><span>m</span></span><span>M</span><span> of Hoechst (33342, #B2261, Sigma) from a 40 </span><span><span>m</span></span><span>M</span><span> stock solution was added 15 minutes before imaging to each well. Nuclei image fluorescence readings were taken using the same Olympus 4x UPLFLN objective and a 365 nm LED with an EX337/EM447 DAPI filter cube.</span></p> <p> </p>
Optimising C2C12 muscle myoblast transfection with polyethylenimine (PEI) or Lipofectamine-2000
<p>Optimisation of DNA transfection of the C2C12 muscle myoblast cell line, comparing use of polyethylenimine (PEI) and Lipofectamine-2000 with varying treatment conditions. </p>
Efficacy of inhibitor on wild-type ACVR1 and R206H mutant in C2C12 cells (by DLA)
<p>There are concerns that compounds that are effective in inhibiting ALK2 by occupying its ATP-binding pocket might have reduced efficacy against mutant ALK2. That will be undesirable since the compounds should also target the mutant gain-of-function ALK2 in DIPG cells. The following experiment takes advantage of the fact that ALK2-R206H mutant confers a neofunction of being activated by Activin A (not activating towards wild-type ALK2). Therefore, using Activin A as the stimulation ligand, the effectiveness of inhibition on ALK2-R206H can be specifically determined. BMP6 which activates both wild-type ALK2 and ALK2-R206H is also included.</p>
Determining the suitability of C2C12 and HEK293 in dual luciferase assay (DLA) for ACVR1 (ALK2) and TGFBR1 (ALK5)
<p>Aside from having high potency towards ACVR1/ALK2, inhibitor compounds ideally should be highly selective and not target other members of the Transforming Growth Factor beta (TGFb) superfamily. TGFBR1/ALK5 is selected for off-target screening because of its potential role in cardiac functions.</p>
Gene Enhancer Predictions in C2C12 (Mouse Myoblast)
<p>This file contains the complete enhancer prediction output of the analysis done in this study published on Nature Communications:</p> <p><a href="https://www.nature.com/articles/s41467-025-57758-x" target="_blank" rel="noopener">https://www.nature.com/articles/s41467-025-57758-x</a></p> <p>using the Activity by Contact Model following the pipeline described here: <a href="https://github.com/broadinstitute/ABC-Enhancer-Gene-Prediction">https://github.com/broadinstitute/ABC-Enhancer-Gene-Prediction</a></p> <p>To be used in this analysis, a genomewide Hi-C interaction matrix was generated with <a>Juicer </a> v1.6, expression counts for each gene were generated with STAR v2.7.10b and <a>Rsubread</a> v2.8.2, and the sequence alignment maps of ATAC-seq and H3K27ac ChIP-seq were generated with NextGenMap v0.5.5, all using raw sequencing reads downloaded from <a>SRA</a>: Hi-C (SRR16220088), RNA-seq (SRR074113 and SRR074114), <a>ATAC-seq (SRR2999996) and H3K27ac ChIP-seq (SRR358589, SRR358590, and SRR358591).</a></p> <p><a>The chromosome coordinates are of the GRCm38 - mm10 assembly, and the gene IDs are from ENSEMBL annotation.</a></p> <p>Please use the Version 3, and for more information please refer to our publication that used these enhancer predictions.</p>
Fig. 5. C2C12 in Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium
Fig. 5. C2C12 myotube cells were exposed to compounds 2 and 4 (5 μM and 20 μM, respectively) and incubated for 1 h. The phosphorylation of the protein AMPK in the cells was assessed by Western blot analysis. Fold expression was calculated as p-AMPK/AMPK and normalized to the protein level of β-actin. Values are expressed as the mean ± SD (n = 3).
Gene Enhancer Predictions in C2C12 (Mouse Myoblast)
<p>This file contains the complete enhancer prediction output of the analysis done in this study published on Nature Communications:</p> <p><a href="https://www.nature.com/articles/s41467-025-57758-x" target="_blank" rel="noopener">https://www.nature.com/articles/s41467-025-57758-x</a></p> <p>using the Activity by Contact Model following the pipeline described here: <a href="https://github.com/broadinstitute/ABC-Enhancer-Gene-Prediction">https://github.com/broadinstitute/ABC-Enhancer-Gene-Prediction</a></p> <p>To be used in this analysis, a genomewide Hi-C interaction matrix was generated with <a>Juicer </a> v1.6, expression counts for each gene were generated with STAR v2.7.10b and <a>Rsubread</a> v2.8.2, and the sequence alignment maps of ATAC-seq and H3K27ac ChIP-seq were generated with NextGenMap v0.5.5, all using raw sequencing reads downloaded from <a>SRA</a>: Hi-C (SRR16220088), RNA-seq (SRR074113 and SRR074114), <a>ATAC-seq (SRR2999996) and H3K27ac ChIP-seq (SRR358589, SRR358590, and SRR358591).</a></p> <p><a>The chromosome coordinates are of the GRCm38 - mm10 assembly, and the gene IDs are from ENSEMBL annotation.</a></p> <p> </p> <p>This research was funded in whole or in part by the Austrian Science Fund (FWF) [P29713-B28, P32512-B and P36503-B] to Roland Foisner and a doctorate program funded by the Austrian Science Fund (FWF) [W1261-B28].</p> <p> </p> <p>For more information please refer to our publication that used these enhancer predictions titled:</p> <p>MyoD1 localization at the nuclear periphery is mediated by association of WFS1 with active enhancers</p>
Transcriptional profiling of MLL5 Knock down vs. control C2C12 myoblasts (S phase enriched)
GEO Series GSE14931. Mus musculus. 2 samples. Type: Expression profiling by array.
C2C12 expressing desmin: WT vs Mutations R406W and I451M
GEO Series GSE185589. Mus musculus. 4 samples. Type: Expression profiling by array.
Genome-wide analysis of mRNA expression in control and ChRO1 depleted C2C12 (Myoblasts and Myotubes)
GEO Series GSE104304. Mus musculus. 4 samples. Type: Expression profiling by array.
RNA-Seq analysis of Mapk14 knockout and myogenic differentiation in C2C12 cells
GEO Series GSE315765. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
Expression analysis of C2C12 cells treated with scramble siRNA or siRNA directed against Fndc1
GEO Series GSE217125. Mus musculus. 10 samples. Type: Expression profiling by high throughput sequencing.
long read RNA-seq from C2C12 (ENCSR221XGR)
GEO Series GSE219595. Mus musculus. 2 samples. Type: Expression profiling by high throughput sequencing.
RNA-sequencing analysis to investigate genes regulated by CASZ1 in C2C12 cells
GEO Series GSE126144. Mus musculus. 15 samples. Type: Expression profiling by high throughput sequencing.
meRIP-seq and RNA-seq on proliferating and differentiating cultured C2C12 cells
GEO Series GSE154720. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing; Other.
Transcriptomic response of ginsenoside Rc (gRc) in C2C12 myotube under dexamethasone (DEX) treatment
GEO Series GSE261787. Mus musculus. 18 samples. Type: Expression profiling by high throughput sequencing.
Enzymatic treatment using trypsin and cold-active protease for C2C12 myoblasts
GEO Series GSE168452. Mus musculus. 18 samples. Type: Expression profiling by high throughput sequencing.
Identification of targets genes of PRDM2 in G0 arrested myoblasts of C2C12
GEO Series GSE58748. Mus musculus. 3 samples. Type: Genome binding/occupancy profiling by array.
polyA mRNA RNA-seq from C2C12 (ENCSR000AHY)
GEO Series GSE90173. Mus musculus. 1 samples. Type: Expression profiling by high throughput sequencing.
long read RNA-seq from C2C12 (ENCSR418ZYU)
GEO Series GSE219813. Mus musculus. 2 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.