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7 results for “carbon isotope analysis”
PIE LTER, stable isotope chemistry (carbon, nitrogen and sulfur) for food web analysis of functional groups in the Plum Island Sound Estuary, Massachusetts.
Stable isotopes of primary producers will be compared to stable isotopes of functional groups of organisms at primarily three sites within the estuary that have different dominant sources of organic matter. The three sites are: Lower (IBYC, SO-3, mouth of Plum Island Sound, 2-3 km upstream of the mouth of the estuary), Middle (OTL, PR-10.1, upper Sound, lower Parker, 8-11 km upstream of the mouth of the estuary) and Upper (P2, PR-21.9, upper Parker, above Middle Rd Bridge (22 km upstream of the mouth of the estuary). The Lower site is dominated by marine phytoplankton, the Middle site is dominated by a mixture of salt marsh and phytoplankton and the Upper site is dominated by oligohaline phytoplankton and fresh marsh. Ten functional groups will be sampled at each site (Surface sediment, benthic diatoms, Nereis, mummichog, ribbed mussels, POM, blue mussels, pelagic copepods (Acartia), silversides and soft shell clams (Mya). Marsh, benthic algae and phytoplankton inputs or benthic vs. pelagic pathways will be evident in the isotopic signals of these functional groups. Samples will be collected between the middle and end of August to reflect a growing season using recently produced OM. Samples of 15 – 20 individuals will be pooled for analysis. Some silverside samplings will have 3 different pooled samples for determination of variance. Often times the same species are not collected at each site due to habitat differences (salinity/discharge) so additional species are collected to try to accomplish task of getting functional groups collected.
Acid fractionation during carbonate digestion with phosphoric acid – Assessment of two different techniques applied for clumped and stable isotope analysis using a Tuneable Infrared Laser Differential Absorption Spectrometer (TILDAS) [dataset]
<p>Date files : </p> <table> <tbody> <tr> <td> <p>Data 1 (Relationship between Mixing ratio difference of sample and WRG and D638 value).xlsx;Data 2 (Role of bulk isotope composition (δ628 & δ636) on Δ638).xlsx;Data 3 (CDES scale conversion).xlsx;Data 4 (VPDB scale conversion for d628 and d636).xlsx;Data 5 (Reference material values).xlsx;Data 6 (Break seal method acid fractionation factor of calcite).xlsx;Data 7 (IAB method acid fractionation factor of calcite).xlsx</p> </td> </tr> </tbody> </table>
Stable carbon and nitrogen isotope analysis data for sequentially sampled whiskers of caracals (Caracal caracal) on the Cape Peninsula, South Africa
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Paired X-ray Micro CT Scanning and individual foraminifera isotopic analysis reveal (de)coupled changes in carbonate preservation and temperature
<p>To assess the coupling between drivers of isotopic variability and carbonate dissolution in foraminifera, we combine individual foraminiferal carbon and oxygen isotopic analyses (IFA) and X-ray MicroCT Scanning (XMCT)-based measurements of test densities (a proxy for the extent of post-depositional dissolution). As a proof-of-concept application of this approach, we analyze Globigerina bulloides tests from both coretop and downcore (latest Miocene/earliest Pliocene-aged) sediment from Ocean Drilling Project (ODP) Site 1088 (Agulhas Ridge).<br>Sediments from both the core intervals were wet-sieved over a 150 μm sieve and oven-dried at 55°C overnight. Individual tests of G. bulloides were picked from the >150 μm size fraction and gently brushed with the wet tip of a paintbrush to remove any adhering clay. Broken or partially fragmented tests were avoided. 25-50 individuals were mounted on a 1.2cm x 1 cm cardboard sample holder using double-sided carbon and imaged at a time on a Bruker Skyscan 1272 Micro-CT Scanner. In total, 123 individuals from the coretop sample and 88 individuals from the downcore sample were scanned.<br>Stable carbon (δ¹³C) and oxygen (δ¹⁸O) isotopic compositions of individual foraminifera were measured using a Kiel Carbonate Device IV coupled to a Thermo Fisher MAT 253 Plus Isotope Ratio Mass Spectrometer (IRMS). All individuals were weighed on a Sartorius ultramicrobalance before analysis, and only samples with mass >10 µg were analyzed as per the long-term precision setup of analyzing small quantities of carbonates on this machine. The XMCT-IFA dataset in this study consists of δ¹³C and δ¹⁸O values of 90 foraminifera (67 coretop and 23 downcore individuals). We compare population averages of XMCT parameters and isotopic compositions from both the coretop and downcore sediments.</p>
Data from: Starvation effects on nitrogen and carbon stable isotopes of animals: an insight from meta-analysis of fasting experiments
Nitrogen and carbon stable isotopic compositions (δ15N and δ13C) of consumers have been used for physiological and food web studies. Previous studies have shown δ15N and δ13C values are affected by several biological and environmental factors during starvation, but the generality of the effect of starvation on δ15N and δ13C values has not yet been tested. Here, we performed a meta-analysis to evaluate the effects of starvation on δ15N and δ13C values of consumers, and the underlying factors that may explain the observed variation. The δ15N and δ13C values were calculated as the differences between the final δ15N and δ13C values of consumers (post-starvation) and the pre-starvation values on each experiment. Our meta-analysis showed a large variation in the δ15N and δ13C values of consumers (δ15N range: –0.82 to 4.30‰; mean: 0.47‰ and δ13C range: –1.92 to 2.62‰; mean: 0.01‰). The δ15N values of most consumers increased along the length of the starvation period and were influenced by nitrogen excretion and thermoregulation types, probably because differences in nitrogen metabolism and thermoregulation affect nitrogen processing and excretion rates. None of our predictor variables accounted for the variation in δ13C values, which showed both increases and decreases due to fasting. Our findings suggest that starvation results in changes in consumer δ15N values which are mainly explained by the length of the fasting period and by nitrogen and energy metabolism, but the underlying mechanisms of the starvation effects on δ13C values seem to be more complex than previously thought.
Data from: Starvation effects on nitrogen and carbon stable isotopes of animals: an insight from meta-analysis of fasting experiments
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Data from: Quantifying terrestrial carbon in freshwater food webs using amino acid isotope analysis—case study with an endemic cave fish
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