Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
64
datasets available to search
ShareScore release 0.9.0
Dataset results
64 results for “chloroplast sequence”
Simulated Arabidopsis thaliana sequencing datasets for chloroplast assembler benchmarking
<p><strong>Changes</strong></p> <ul> <li>Fixed non-circular sampling from chloroplast and mitochondrion in version 1.1.0</li> <li>Fixed off-by-one error in reverse read in version 1.0.0</li> </ul> <p><strong>Purpose and Documentation</strong></p> <p>See: <a href="https://github.com/chloroExtractorTeam/benchmark">github.com/chloroExtractorTeam/benchmark</a></p> <p><strong>Original data</strong><br> The original <em>Arabidopsis thaliana </em>sequences were downloaded from TAIR: </p> <p>The Arabidopsis Information Resource (<a>TAIR</a>) on www.arabidopsis.org, Mar 22, 2019 available under the <a href="http://www.arabidopsis.org/doc/about/tair_terms_of_use/417">TAIR Terms of Use</a> </p> <p><em>Tanya Z. Berardini, Leonore Reiser, Donghui Li, Yarik Mezheritsky, Robert Muller, Emily Strait and Eva Huala. "The Arabidopsis Information Resource: Making and mining the "gold standard" annotated reference plant genome." genesis 2015 <a href="https://doi.org/10.1002/dvg.22877">doi:10.1002/dvg.22877</a></em></p> <p><strong>Programs used to generate this data</strong><br> - <a href="https://github.com/shenwei356/seqkit">seqkit</a> (v0.10.1): Shen W, Le S, Li Y, Hu F (2016) "SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation." PLOS ONE 11(10): e0163962. <a href="https://doi.org/10.1371/journal.pone.0163962">doi:10.1371/journal.pone.0163962</a></p> <p> </p>
Fig. 3 in Evolution of Polyscias sect. Tieghemopanax (Araliaceae) based on nuclear and chloroplast DNA sequence data
Fig. 3. — Strict consensus of 10,000 most parsimonious trees resulting from the analysis of 58 ITS sequences; tree length = 725 steps; consistency index = 0.518; retention index = 0.734. Clades denoted by brackets are those referred to in text. Values along branches are bootstrap percentages. Placement of Polyscias elegans (discussed in text) is denoted by an asterisk. Labels for the Tieghemopanax group and other clades in "Polyscias sensu lato" follow PLUNKETT et al. (2001).
Fig. 2 in Evolution of Polyscias sect. Tieghemopanax (Araliaceae) based on nuclear and chloroplast DNA sequence data
Fig. 2. — The single most parsimonious tree resulting from the combined analysis of ITS + trnL-trnF + 5S spacer sequences, based on the identical 24-taxon sampling as used in Fig. 1; tree length = 349 steps; consistency index = 0.663; retention index = 0.770. Values along nodes are bootstrap percentages; nodes with dashed branches have bootstraps below 70%. Identical or near identical clades (labeled A-E) from Fig. 1 are also labeled, as is the placement of Polyscias sambucifolia and P. microbotrys (asterisks), as discussed in text. Outgroups indicated by "OG."
Fig. 4 in Evolution of Polyscias sect. Tieghemopanax (Araliaceae) based on nuclear and chloroplast DNA sequence data
Fig. 4. — Strict consensus of 156 most parsimonious trees resulting from the analysis of 40 trnL-trnF sequences; tree length = 122 steps; consistency index = 0.938; retention index = 0.972. Values along branches are bootstrap percentages. Placement of Polyscias elegans (discussed in text) is denoted by an asterisk. Labels for the Tieghemopanax group and other clades in "Polyscias sensu lato" follow PLUNKETT et al. (2001).
Fig. 5 in Evolution of Polyscias sect. Tieghemopanax (Araliaceae) based on nuclear and chloroplast DNA sequence data
Fig. 5. — Strict consensus of 156 most parsimonious trees resulting from the analysis of 26 5S spacer sequences; tree length = 147 steps; consistency index = 0.744; retention index = 0.849. Values along branches are bootstrap percentages.
Chloroplast genome sequencing reads from snow gum
<p>Tutorial data for chloroplast genome assembly: fastq reads from illumina and nanopore sequencing for the snow gum, <em>Eucalyptus pauciflora</em>.</p> <p>Data from: Wang, W., Schalamun, M., Morales-Suarez, A. et al. Assembly of chloroplast genomes with long- and short-read data: a comparison of approaches using Eucalyptus pauciflora as a test case. BMC Genomics 19, 977 (2018) doi:10.1186/s12864-018-5348-8</p> <p>Data hosted at NCBI under accession numbers: illumina (SRR7153063) and nanopore (SRR7153095). Additional illumina file SRR7153071 not used here. </p> <p>This is how the files have been changed from the original datasets: </p> <p>Using the Galaxy platform (usegalaxy.org): </p> <ul> <li> <p>Each dataset was separately mapped to the NCBI Reference Sequence for <em>Eucalyptus pauciflora </em>chloroplast NC_039597.1, using BWA-MEM. </p> </li> <li> <p>Unmapped reads were filtered out using a SAMtools flag. </p> </li> <li> <p>Bam files were converted to fastq files.</p> </li> <li> <p>Each fastq file was then reduced in size:</p> </li> <li> <p>snow-gum-illumina-cp-reduced: has the first 62,500 reads only. Note that original pairing of reads has not been preserved so consider these to be unpaired reads for this tutorial.</p> </li> <li> <p>snow-gum-nanopore-cp-reduced: has only reads that are longer than 90,000 bp.</p> </li> </ul>
The alignments of chloroplast genome sequences and nuclear ribosomal DNA fragments of six oak species sampled in the hot-dry valley of the Jinsha River, southwestern China
<p>Both chloroplast (cp) genome sequences and nuclear ribosomal (nr) DNA were assembled using GetOrganelle v.1.7.6.1 for 18 oak trees sampled in the Panzhihua Cycad National Nature Reserve, Sichuan Province, China. These trees belong to six oak species, including Quercus cocciferoides, Q. dolicholepis, Q. franchetii, Q. griffithii, Q. longispica, and Q. variabilis. We used PhyloSuite v.1.1.152 to extract coding sequences (CDSs), tRNA genes, rRNA genes, introns, and intergenic spacers (IGSs) of the 18 oak cp genomes. These sequences were aligned separately using MAFFT v.7.3.13 and manually adjusted with BioEdit v.7.2.5. Length variations in mononucleotide repeats were excluded and inversions were replaced with their reverse complements because of their tendency for homoplasy. Other indels were coded as binary characters according to the simple gap coding method using GapCoder. Separate assignments were concatenated according to their respective positions in the cp genome to obtain the alignments of LSC, SSC, IRb, and the whole cp genome.</p>
Supplementary Materials from the article Characterization and molecular evolution analysis of Periploca forrestii inferred from its complete chloroplast genome sequence
<p>Table S1. Base composition of chloroplast genome in <em>P. forrestii</em>, Table S2. The lengths of introns and exons for the splitting genes, Table S3. The GC content of the codons from <em>P. forrestii </em>chloroplast genome, Table S4. Preferred codons in chloroplast genome of <em>P. forrestii</em>, Table S5. Long repeat sequences in the <em>P. forrestii </em>chloroplast genome, Figure S1. Codon bias analysis of P. forrestii chloroplast genome. (A) Neutrality plot analysis; (B) Analysis of PR2 bias plot; (C) Analysis on ENC and GC3 relationship.</p>
Microsatellite data, chloroplast and nuclear rRNA sequences of Avicennia marina from Vietnam, Malaysia, and The Philippines
Open the record for dataset details and reuse information.
Concatenated data matrix of DNA sequences from two nuclear and four chloroplast gene regions
Open the record for dataset details and reuse information.
The data of complete chloroplast genome sequence of Sorbus amabilis (Rosaceae) in China
<p>This dataset includes the complete chloroplast genome of <em>Sorbus amabilis </em> in China.</p>
DNA sequences for six chloroplast loci concatenated, representing haplotypes found in Colocasia esculenta, and closely related Araceae
<p><span>As an ancient clonal root and leaf crop, taro (<i>Colocasia esculenta</i>) is highly polymorphic with uncertain genetic and geographic origins. We explored chloroplast DNA variation in wild and cultivated taros and other <i>Colocasia</i> species, and found cultivated taro to be polyphyletic, with tropical and temperate clades originating in Southeast Asia. A third clade was found exclusively in wild populations from Southeast Asia to Australia and Papua New Guinea. Our findings do not support the hypothesis of taro domestication in Papua New Guinea. </span></p>
Data from: Assemblage Accumulation Curves: A framework for resolving species accumulation in biological communities using chloroplast genome sequences
The timing and tempo of the processes involved in community assembly are of substantial concern to community ecologists and conservation managers. The fossil record is a valuable source of data for studying past changes in community composition, but it is not always detailed enough to allow the process of community assembly to be resolved at regional or site scales while tracing the trajectories of known species with associated known traits. We present a three‐step framework for studying present‐day species accumulation through time: DNA sampling from multiple individuals from multiple species within a community; estimates of coalescence times for each species using molecular dating methods; and plotting the accumulation of present‐day species through time using the inferred population ages. Our approach is illustrated using whole chloroplast genomes from plants from three rainforest communities in eastern Australia. Expected times to coalescence for multiple species in each community were inferred from pooled high‐throughput sequence libraries. Local assemblage accumulation curves for each community were constructed. We also explored the variation in assemblage accumulation curves of species with different functional traits. Models of equilibrium species richness informed our null hypothesis and largely explained the shape of the assemblage accumulation curves and indicated that the complexities of the accumulation process should be explored with additional parameters, for example allowing species classes with different extinction rates. The assemblage accumulation curves for the study sites showed evidence of recent population expansions within each of the communities. This signal of recent accumulation is consistent with the increase in suitable rainforest habitat that followed the Last Glacial Maximum. Our method of constructing assemblage accumulation curves provides a simple approach for visualizing species‐accumulation data. It can be used to test hypotheses such as the relative survival potential of species‐specific ecological attributes. Although our example used single‐nucleotide polymorphisms derived from whole‐chloroplast sequencing, this framework can be applied to mitochondrial genomes and to communities of other organisms.
Data from: Phylogenetic inferences using nuclear ribosomal ITS and chloroplast sequences provide insights into the biogeographic origins, diversification timescales and trait evolution of Rubus in the Japanese Archipelago
<p><span>This study aimed to reveal the evolutionary timescale and processes underlying the diversity of <em>Rubus</em> in the Japanese Archipelago. We conducted molecular phylogenetic analyses of most native species (35 species), along with previously published data from 116 foreign species, based on nuclear ribosomal internal transcribed spacer (ITS) and chloroplast DNA sequences. Most of the northern species of Japan, that is, <em>R. chamaemorus</em>, <em>R. pedatus</em>, <em>R. vernus</em>, <em>R. pseudojaponicus</em>, and <em>R. ikenoensis</em>, were found to belong to anciently diverged lineages; in particular, <em>R. ikenoensis</em> formed a unique lineage distinct from other species. The other species diverged into two evolutionary groups. One included subg. <em>Malachobatus</em>, <em>Chamaebatus</em>, and sects. <em>Pungentes</em>, <em>Idaeanthi</em>, and <em>Parvifolii</em> (subg. <em>Idaeobatus</em>), which was further divided into two clades in the chloroplast phylogenies. Although the phylogenetic structures within this group were unresolved, <em>R. sieboldii</em> has been proven to be recently derived. The second group represented a well-supported clade, comprising sects. <em>Microphylli</em>, <em>Corchorifolii</em>, <em>Peltati</em>, and <em>Rosifolii</em> (subg. <em>Idaeobatus</em>), and suggested early Miocene diversification of this Asian lineage associated with character specialization in vegetative reproduction and leaf shape. This clade was further resolved into lower clades primarily representing the sectional classifications, although the placement of the earliest diverged species, <em>R. sumatranus</em>, <em>R. peltatus</em>, <em>R. corchorifolius</em>, and <em>R. chingii</em>, was incongruent among gene trees. At the lower taxonomic levels, <em>R. illecebrosus</em>, <em>R. grayanus</em>, and the thornless species of sect. <em>Microphylli</em> showed earlier divergence.</span></p>
FIGURE 2 in Is Ronnbergia (Bromeliaceae, Bromelioideae) a geographically disjunct genus? Evidence from morphology and chloroplast DNA sequence data
FIGURE 2. Majority rule consensus tree from the BI analysis of the "total evidence" dataset from morphological and molecular data. Black lines represent the branches that remained identical in the strict consensus tree of the MP analysis. Grey lines represent branches obtained only in the BI analysis. Numbers in the nodes correspond to posterior probabilities and bootstrap values (PP/BT); dashes (-) represent low (<50%) or no BT support.
FIGURE 1 in Is Ronnbergia (Bromeliaceae, Bromelioideae) a geographically disjunct genus? Evidence from morphology and chloroplast DNA sequence data
FIGURE 1. Comparison of the phylogenetic hypothesis provided by the independent morphological and molecular datasets. Species of Ronnbergia are highlighted in red. A. Strict consensus of the four most parsimonious trees from the MP analysis of morphological data. Numbers in the nodes correspond to bootstrap values. B. Majority rule consensus tree from the BI analysis of the molecular dataset. Numbers in the nodes correspond to posterior probabilities and bootstrap values (PP/BT); dashes (-) represent low (<50%) or no BT support.
FIGURE 3 in Is Ronnbergia (Bromeliaceae, Bromelioideae) a geographically disjunct genus? Evidence from morphology and chloroplast DNA sequence data
FIGURE 3. Geographic distribution of the three clades containing species of Ronnbergia. Data points were obtained from the Global Biodiversity Information Facility—GBIF (www.gbif.org).
FIGURE 2 in Phylogenetic position and independent generic status of Indocypraea (Asteraceae-Heliantheae-Ecliptinae): evidence from chloroplast DNA sequences
FIGURE 2. Phylogeny of the subtribe Ecliptinae (Asteraceae: Heliantheae) based on chloroplast DNA sequences by using maximum likelihood analysis. Bootstrap values (≥ 70%; maximum likelihood/maximum parsimony) are indicated above branches, and posterior probabilities (≥ 0.95) below branches.
FIGURE 1. Indocypraea montana. A. Habit. B in Phylogenetic position and independent generic status of Indocypraea (Asteraceae-Heliantheae-Ecliptinae): evidence from chloroplast DNA sequences
FIGURE 1. Indocypraea montana. A. Habit. B. Flowering capitulum (top view). C. Flowering capitulum (lateral view). D. Fruiting capitulum. E. Achene (lateral view). F. Achene (top view). A–C from Huaiji, Guangdong, China (L.Y. Wang & M. Tang 106, IBSC); D from Lingui, Guangxi, China (M. Tang & L.Y. Wang 380, IBSC); E, F from Changjiang, Hainan, China (Z.X. Li 3850, IBSC).
FIGURE 2 in Molecular phylogeny of Faberia (Asteraceae: Cichorieae) based on nuclear and chloroplast sequences
FIGURE 2. Strict consensus tree of Faberia and relatives based ITS sequences. Tree length = 1227 steps, CI = 0.50, RI = 0.55, and RC = 0.28. Bootstrap values greater than 50% are above the lines and Bayesian posterior probabilities are below the lines.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.