Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
139
datasets available to search
ShareScore release 0.9.0
Dataset results
139 results for “chloroquine”
pH-dependence of the Plasmodium falciparum chloroquine resistance transporter is linked to the transport cycle
<p>This file contains the original data underpinning the following study:</p> <p>The chloroquine resistance transporter, PfCRT, of the human malaria parasite <em>Plasmodium falciparum </em>displays a strong pH-sensitivity in the weakly acidic range. Consequently, PfCRT operates only at 60% of its maximal drug transport activity at the pH of 5.2 of the digestive vacuole, a proteolytic organelle from which PfCRT expels drugs that interfere with heme detoxification. Despite structural information, the molecular mechanism by which PfCRT senses pH changes has remained unclear. Here we show, by alanine-scanning mutagenesis and functional transport studies, that E207 plays a critical role in pH sensing. The E207A mutant displayed a pH-insensitive transport activity, while preserving drug substrate specificity. Replacement of E207 by Asp or His, but not by any other proteinogenic amino acid, reconstituted pH sensitivity. Molecular dynamics simulations and kinetics analyses suggest an allosteric binding model in which PfCRT can simultaneously accept both protons and chloroquine in a partial non-competitive manner, with increasing proton concentrations reducing the drug transport activity. Molecular dynamics simulations of the open-to-vacuole, the occluded and the open-to-cytosol conformation of PfCRT revealed a drastic relocation of E207 from a peripheral to an engaged location during the transport cycle, resulting in E207 forming a salt bridge with residue K80. We propose that the ionized carboxyl group of E207 acts as a hydrogen acceptor for interactions accelerating progression through the transport cycle and that the pH sensing is a by-product of this function.</p>
Convergent evolution of artemisinin and chloroquine resistance in Ethiopian Plasmodium falciparum parasites
Open the record for dataset details and reuse information.
pH-dependence of the Plasmodium falciparum chloroquine resistance transporter is linked to the transport cycle
Open the record for dataset details and reuse information.
Data from Mehra et al. (2020-05-29), "Hydroxychloroquine or chloroquine with or without a macrolide for treatment of COVID-19: a multinational registry analysis"
<p>Recent <a href="http://doi.org/dw8t">reports</a> have identified errors and implausible results in the <a href="http://doi.org/ggwzsb">title paper</a>, few of which have been corrected to date. To facilitate analysis, I provide a spreadsheet containing all data from tables in the corrected version (2020-05-29), from both the main text (html) and supporting information (PDF).</p> <p><br> The data is uploaded to this publicly-funded repository with the <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255293/">permission</a> of the copyright holder: <em>"Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories ... with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source."</em> Text and data mining are also <a href="http://web.archive.org/web/20200602210529/https://www.elsevier.com/connect/coronavirus-information-center">permitted</a>: <em>"These articles are also available to download with rights for full text and data mining, re-use and analyses for as long as needed."</em></p>
The knock-down of the chloroquine resistance transporter PfCRT is linked to oligopeptide handling in Plasmodium falciparum
<p class="MsoNormal">The chloroquine resistance transporter, PfCRT, is an essential factor during intraerythrocytic development of the human malaria parasite <em>Plasmodium falciparum</em>. PfCRT resides at the digestive vacuole of the parasite, where hemoglobin taken up by the parasite from its host cell is degraded. PfCRT can acquire several mutations that render PfCRT a drug transporting system expelling compounds targeting hemoglobin degradation from the digestive vacuole. The non-drug related function of PfCRT is less clear, although a recent study has suggested a role in oligopeptide transport based on studies conducted in a heterologous expression system. The uncertainty about the natural function of PfCRT is partly due to a lack of a null mutant and a dearth of functional assays in the parasite. Here, we report on the generation of a conditional PfCRT<em> </em>knock-down mutant in <em>P. falciparum</em>. The mutant accumulated oligopeptides 2 to at least 8 residues in length under knock-down conditions, as shown by comparative global metabolomics. The accumulated oligopeptides were structurally diverse, had an isoelectric point between 4.0 and 5.4 and were electrically neutral or carried a single charge at the digestive vacuolar pH of 5.2. Fluorescently-labeled dipeptides and live cell imaging identified the digestive vacuole as the compartment where oligopeptides accumulated. Our findings suggest a function of PfCRT in oligopeptide transport across the digestive vacuolar membrane in <em>P. falciparum</em> and associated with it a role in nutrient acquisition and the maintenance of the colloid osmotic balance.</p>
Docking of chloroquine and piperaquine to PfCRT_Dd2 and to the PfCRT_Dd2 mutations conferring piperaquine resistance
<p>PDB files of the docking of chloroquine and piperaquine to the PfCRT_Dd2 variant and to the Dd2 mutants investigated</p> <p>reported in the manuscript</p> <p>"PfCRT mutations conferring piperaquine resistance in falciparum malaria shape the kinetics of quinoline drug binding and transport"</p> <p>by Guillermo Martin Gomez; Giulia D'Arrigo; Fiona Berger; Cecilia P. Sanchez; Rebecca C. Wade and Michael Lanzer</p> <p>submitted to PLOS Pathogens</p> <p> </p> <p>1. <strong>Dockings.zip </strong>contains <strong>a)</strong> the individual pdb files of the PfCRT_Dd2 variants models prior to preparation with maestro and <strong>b)</strong> sub-folders with the docking results for each PfCRT_Dd2 variant and for PfCRT_7G8</p> <p>2. <strong>Simulations.zip </strong>contains the input file for the simulated systems. Each sub-folder contains:</p> <ul> <li>assembly.pdb - structure of the complex in PDB format</li> <li>assembly.prmtop - topology file in AMBER</li> <li>assembly.inpcrd - coordinates file in AMBER</li> <li>final.pdb - structure after NPT production</li> </ul>
Chloroquine Alone or in Combination for Malaria in Children in Malawi
ClinicalTrials.gov study NCT00379821. IPD Sharing: Not stated. Countries: 1. Publications: 1.
TES of Artemether-lumefantrine for Pf and Chloroquine for Pv in the Philippines From 2013-2014
ClinicalTrials.gov study NCT04222088. IPD Sharing: YES. Countries: 0. Publications: 2.
Chloroquine in Combination With VELCADE and Cyclophosphamide for Relapsed and Refractory Multiple Myeloma
ClinicalTrials.gov study NCT01438177. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Evaluate Azithromycin Plus Chloroquine And Sulfadoxine Plus Pyrimethamine Combinations For Intermittent Preventive Treatment Of Falciparum Malaria Infection In Pregnant Women In Africa
ClinicalTrials.gov study NCT01103063. IPD Sharing: Not stated. Countries: 5. Publications: 2.
Sanaria PfSPZ Challenge With Pyrimethamine or Chloroquine Chemoprophylaxis Vaccination (PfSPZ-CVac Approach): A Randomized Double Blind Placebo Controlled Phase I/II Trial to Determine Safety and Prot
ClinicalTrials.gov study NCT03952650. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Chloroquine/ Hydroxychloroquine Prevention of Coronavirus Disease (COVID-19) in the Healthcare Setting
ClinicalTrials.gov study NCT04303507. IPD Sharing: YES. Countries: 10. Publications: 4.
Daily Trimethoprim-sulfamethoxazole or Weekly Chloroquine Among Adults on ART in Malawi
ClinicalTrials.gov study NCT01650558. IPD Sharing: NO. Countries: 1. Publications: 3.
Evaluation of the Immune Response of a HIV Candidate Vaccine After Administration of One Chloroquine Dose
ClinicalTrials.gov study NCT00972725. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Azithromycin Plus Chloroquine Versus Artemether-Lumefantrine For The Treatment Of Uncomplicated P. Falciparum Malaria In Children In Africa
ClinicalTrials.gov study NCT00677833. IPD Sharing: Not stated. Countries: 5. Publications: 1.
Chloroquine for Reducing Immune Activation in HIV- Infected Individuals
ClinicalTrials.gov study NCT00819390. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Study of the Efficacy of Chloroquine in the Treatment of Ductal Carcinoma in Situ (The PINC Trial)
ClinicalTrials.gov study NCT01023477. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Efficacy of 3 Regimens of Chloroquine and Primaquine for Treatment of P. Vivax Malaria, Cruzeiro do Sul, Acre, Brazil
ClinicalTrials.gov study NCT03610399. IPD Sharing: NO. Countries: 1. Publications: 1.
Chloroquine to Treat People With Metabolic Syndrome Aim2 (ARCH-MS)
ClinicalTrials.gov study NCT00455325. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Atheroma Reduction With Chloroquine in Patients With the Metabolic Syndrome (ARCH-MS)
ClinicalTrials.gov study NCT00455403. IPD Sharing: Not stated. Countries: 1. Publications: 2.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.