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5 results for “comet assay”

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zenodo28/100

Figure 3 from: Gonçalves MW, Gambale PG, Godoy FR, Alves AA, Rezende PH, Cruz AD, Maciel NM, Nomura F, Bastos RP, de Marco-Jr P, Silva DM (2017) The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay. Zoologia 34: 1-8. https://doi.org/10.3897/zoologia.34.e19865

Figure 3 - Principal component analysis (PCA) of the descriptor variables and the covariate pond area. Relationship between the PCA 1 and PCA 2, points were grouped according to the soybean (white triangle), corn (white circles) and non-agricultural lands (black circles). (TL) Tail Length, (% DNA) percentage of DNA in Tail, (OTM) Olive tail moment, (RNV) Remnant native vegetation.

opencc-by-4.0Aug 2017View details →
zenodo28/100

Figures 4-6 from: Gonçalves MW, Gambale PG, Godoy FR, Alves AA, Rezende PH, Cruz AD, Maciel NM, Nomura F, Bastos RP, de Marco-Jr P, Silva DM (2017) The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay. Zoologia 34: 1-8. https://doi.org/10.3897/zoologia.34.e19865

Figures 4-6 - Relationship between comet assay parameters and the pond area according to soybean (gray squares), corn (white circles) and non-agricultural lands (black circles); 4) TL with pond area; 5)% DNA; 6) OTM with pond area. (TL) Tail Length, (% DNA) percentage of DNA in Tail, (OTM) Olive tail moment.

opencc-by-4.0Aug 2017View details →
zenodo28/100

Figure 2 from: Gonçalves MW, Gambale PG, Godoy FR, Alves AA, Rezende PH, Cruz AD, Maciel NM, Nomura F, Bastos RP, de Marco-Jr P, Silva DM (2017) The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay. Zoologia 34: 1-8. https://doi.org/10.3897/zoologia.34.e19865

Figure 2 - Differences between the average scores of Soybean, Corn and Non-agricultural lands, generated by the discriminant function scores.

opencc-by-4.0Aug 2017View details →
zenodo24/100

Comet assay annotation data for comet segmentation and scoring (1037 comet assay images)

<p>Comet assay annotation images used in the following paper</p> <p>Hong, Y., Han, HJ., Lee, H.&nbsp;<em>et al.</em>&nbsp;Deep learning method for comet segmentation and comet assay image analysis.&nbsp;<em>Sci Rep</em>&nbsp;<strong>10,&nbsp;</strong>18915 (2020). https://doi.org/10.1038/s41598-020-75592-7</p> <p>DeepComet online service website:&nbsp;<strong>https://ad3.io/</strong></p> <p>sign up-&gt;dashboard-&gt;APPs-&gt;Deep Comets-&gt;new job</p> <p>&nbsp;</p> <p>Two biology researchers who were experienced in&nbsp;the comet assay for over half a year under the guidance of a toxicology expert in the assay annotated the images independently and compared each other&rsquo;s work to ensure consistency. Here, the <strong>VGG Image Annotator&nbsp;sofware</strong> was used to annotate the images manually. We annotated the intact comet heads without a tail using a circular region tool that outputs the center and radius of the circle. Further, comets with a tail and DNA damage were annotated with a polyline tool, where the frst dot was marked at the center of a comet head, and the second dot was marked at the bottom of the comet head. Tese two points were used to identify the head of a comet. Subsequently, dots were marked along the boundary of the comet tail counterclockwise from the second dot. Te total number of dots were at least eight per each comet.</p> <p>Comets can be sorted into non-ghost cells with a distinct head and tail, and ghost cells with a small or no nucleoid head and a broad tail. The ghost cells are usually classifed as the highest degree of DNA damage by visual scoring. Until now, there is a controversy over the cause of ghost cells and how they should be analyzed appropriately. Therefore, our method is designed to classify these two kinds of cells, so one can use some custom analysis techniques for ghost cells.</p> <p>The comets in our datasets were all classifed as non-ghost or ghost cells. Furthermore, we assigned tags to the comets if they were overlapped or located at the boundary of an image (i.e., only a part of the comet is visible).&nbsp;After manual annotation with dots and tags (in the red box), each dotted contour was flled up to give mask images.</p>

openother-ncMar 2022View details →
zenodo24/100

Figure 1 from: Gonçalves MW, Gambale PG, Godoy FR, Alves AA, Rezende PH, Cruz AD, Maciel NM, Nomura F, Bastos RP, de Marco-Jr P, Silva DM (2017) The agricultural impact of pesticides on Physalaemus cuvieri tadpoles (Amphibia: Anura) ascertained by comet assay. Zoologia 34: 1-8. https://doi.org/10.3897/zoologia.34.e19865

Figure 1 - Spatial distribution of the ponds sampled at five municipalities in the state of Goiás.

opencc-by-4.0Aug 2017View details →

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