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15 results for “cysteine metabolism”
Human adenovirus serotype 5 infection dysregulates cysteine, purine, and unsaturated fatty acid metabolism in fibroblasts
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The critical role of MetR/MetB/MetC/MetX in cysteine and methionine metabolism, fungal development and virulence of Alternaria alternata
<p>Methionine is a unique sulfur-containing amino acid, which plays an important role in biological protein synthesis and various cellular processes. Here, we characterize the biological functions of <em>AaMetB</em>, <em>AaMetC</em>, and <em>AaMetX</em> in the tangerine pathotype of <em>Alternaria alternata</em>. Morphological analysis showed that mutants lacking <em>AaMetB</em>, <em>AaMetC</em>, or <em>AaMetX</em> resulted in less aerial hypha and fewer conidia in artificial media. The virulence assays revealed that <em>AaMetB</em>, <em>AaMetC</em>, and <em>AaMetX </em>are required for full virulence. The defects of <em></em><em>MetB</em>, <em></em><em>MetC</em>, and <em></em><em>MetX</em> in vegetative growth, conidiation and virulence can be restored by exogenous methionine and homocysteine, indicating that <em>AaMetB</em>, <em>AaMetC</em>, and <em>AaMetX </em>are required for methionine biosynthesis. The defects of <em></em><em>MetR </em>in vegetative growth and virulence can be restored by exogenous cysteine, indicating that <em>AaMetR</em> is essential for cysteine biosynthesis. The oxidant sensitivity assay showed that only <em></em><em>MetR</em> is sensitivity to H<sub>2</sub>O<sub>2</sub> and many ROS-generating compounds, which indicates that <em>AaMetR</em> is essential for oxidative tolerance. Interestingly, indoor bioassays of these mutants on fungicides showed that only the <em></em><em>MetR </em>mutants<em> </em>are susceptive to chlorothalonil, which can interact with the cysteine of glyceraldehyde-3-phosphate dehydrogenase. Comparative transcriptome analysis showed that the inactivation of <em>MetB</em>, <em>MetC</em>, <em>MetX</em>, or <em>MetR</em> significantly affected the expression of many genes related to methionine metabolism. Moreover, the inactivation of Aa<em>MetR</em> significantly affected the expression of many genes related to glutathione metabolism, which is required for ROS tolerance. In conclusion, our study provides genetic evidence to define the critical roles of <em>AaMetB</em>, <em>AaMetC</em>, <em>AaMetX</em>, and <em>AaMetR</em> in the metabolism of cysteine and methionine, fungal development and virulence of <em>Alternaria alternata.</em></p>
Cysteine S-acetylation is a post-translational modification involved in metabolic regulation
<p><span>Cysteine is a reactive amino acid central to the catalytic activities of many enzymes. It is also a common target of post-translational modifications (PTMs), such as palmitoylation. This long-chain acyl PTM can modify cysteine residues and induce changes in protein subcellular localization. We hypothesized that cysteine could also be modified by short-chain acyl groups, such as cysteine <em>S</em>-acetylation. To test this, we developed sample preparation and non-targeted mass spectrometry protocols to analyze the mouse liver proteome for cysteine acetylation. Our findings revealed hundreds of sites of cysteine acetylation across multiple tissue types, revealing a previously uncharacterized cysteine acetylome. Cysteine acetylation shows a marked cytoplasmic subcellular localization signature, with tissue-specific acetylome patterns and specific changes upon metabolic stress. This study uncovers a novel aspect of cysteine biochemistry, highlighting short-chain modifications alongside known long-chain acyl PTMs. These findings enrich our understanding of the landscape of acyl modifications and suggest new research directions in enzyme activity regulation and cellular signaling in metabolism.</span></p>
Characterization of PlcRa, a new PlcR-like regulator involved in oxidative stress response and cysteine metabolism during early stationary phase in Bacillus cereus
GEO Series GSE30514. Bacillus cereus ATCC 14579. 8 samples. Type: Expression profiling by array.
Conserved Spermatogonial Gene Networks Couple to Glutathione and Pentose Phosphate Metabolism but not Cysteine Biosynthesis
GEO Series GSE163302. Rattus norvegicus. 8 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
The Food Environment, Microbial Cysteine Metabolism, and Cancer Disparities
ClinicalTrials.gov study NCT07086833. IPD Sharing: NO. Countries: 1. Publications: 0.
N-acetyl-cysteine and Carbohydrate Metabolism Disorder in Obese Women
ClinicalTrials.gov study NCT03902327. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Histone H3 cysteine 110 enhances iron metabolism and modulates replicative life span in Saccharomyces cerevisiae
GEO Series GSE281358. Saccharomyces cerevisiae. 26 samples. Type: Expression profiling by high throughput sequencing.
Inhibition of EZH2 restores normal expression of genes associated with cysteine metabolism and ferroptosis in hepatocellular carcinoma [ATAC-seq]
GEO Series GSE237948. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Hypoxia-induced cysteine metabolism reprogramming are crucial for the tumorigenesis of colorectal cancer [HCT116]
GEO Series GSE223118. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Hypoxia-induced cysteine metabolism reprogramming are crucial for the tumorigenesis of colorectal cancer [tissue pair]
GEO Series GSE223119. Homo sapiens. 40 samples. Type: Expression profiling by high throughput sequencing.
Integration of epigenomic and transcriptomic profiling uncovers EZH2 target genes linked to cysteine metabolism in hepatocellular carcinoma
GEO Series GSE237952. Homo sapiens. 24 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
Inhibition of EZH2 restores normal expression of genes associated with cysteine metabolism and ferroptosis in hepatocellular carcinoma [RNA-seq]
GEO Series GSE237951. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Inhibition of EZH2 restores normal expression of genes associated with cysteine metabolism and ferroptosis in hepatocellular carcinoma [CUT&Tag]
GEO Series GSE268195. Homo sapiens. 12 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Hypoxia-induced cysteine metabolism reprogramming are crucial for the tumorigenesis of colorectal cancer.
GEO Series GSE223120. Homo sapiens. 52 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.