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6 results for “cytosolic calcium”
Additional movies for a manuscript titled 'Leaf epidermal cells respond to application and removal of mechanical force by distinct cytosolic calcium waves'
<p>This repository provides 21 additional videos that highlight the spectrum of mechanically stimulated calcium responses observed in the leaf epidermis of <em>Arabidopsis t</em>. expressing the genetically encoded calcium indicator R-GECO1. These movies correspond to figures in a manuscript entitled 'Leaf epidermal cells respond to application and removal of mechanical force by distinct cytosolic calcium waves'. In each movie, a cantilever exerts approximately 5 mN force to the epidermis (where the cantilever looks like a shadow in the movies). Each movie contains a scale bar and the relative time (hr:min:sec). Placement of the cantilever generally occurs after approximately 1 minute.</p>
Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling
<p><span>L-type Ca<sup>2+</sup> (Ca<sub>V</sub>1) channels transduce channel activities into nuclear signals critical to neuritogenesis. Also, standalone peptides encoded by </span><span><span>Ca<sub>V</sub>1</span> DCT (distal carboxyl-terminus) act as nuclear transcription factors reportedly promoting neuritogenesis. Here, by focusing on exemplary </span><span><span>Ca<sub>V</sub>1</span>.3 and cortical neurons under basal conditions, we discover that cytosolic DCT peptides downregulate neurite outgrowth by the interactions with </span><span><span>Ca<sub>V</sub>1</span>'s apo-calmodulin binding motif. Distinct from nuclear DCT, various cytosolic peptides exert a gradient of inhibitory effects on </span><span>Ca<sup>2+</sup></span><span> influx via CaV1 channels and neurite extension and arborization, and also the intermediate events including CREB activation and c-Fos expression. The inhibition efficacies of DCT are quantitatively correlated with its binding affinities. Meanwhile, c</span><span>ytosolic inhibition tends to facilitate neuritogenesis indirectly by favoring </span><span>Ca<sup>2+</sup>-sensitive nuclear retention of DCT. In summary, DCT peptides as a class of </span><span><span>Ca<sub>V</sub>1</span> inhibitors specifically regulate the channel activity-neuritogenesis coupling in a variant-, affinity-, and localization-dependent manner.</span></p>
Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling
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Data from: Sources of variability in cytosolic calcium transients triggered by stimulation of homogeneous uro-epithelial cell monolayers
Epithelial tissue structure is the emergent outcome of the interactions between large numbers of individual cells. Experimental cell biology offers an important tool to unravel these complex interactions, but current methods of analysis tend to be limited to mean field approaches or representation by selected subsets of cells. This may result in bias towards cells that respond in a particular way and/or neglect local, context-specific cell responses. Here, an automated algorithm was applied to examine in detail the individual calcium transients evoked in genetically homogeneous, but asynchronous populations of cultured non-immortalized normal human urothelial cells when subjected to either the global application of an external agonist or a localized scratch wound. The recorded calcium transients were classified automatically according to a set of defined metrics and distinct sub-populations of cells that responded in qualitatively different ways were observed. The nature of this variability in the homogeneous cell population was apportioned to two sources: intrinsic variation in individual cell responses and extrinsic variability due to context-specific factors of the environment, such as spatial heterogeneity. Statistically significant variation in the features of the calcium transients evoked by scratch wounding according to proximity to the wound edge was identified. The manifestation of distinct sub-populations of cells is considered central to the coordination of population-level response resulting in wound closure.
Data from: Sources of variability in cytosolic calcium transients triggered by stimulation of homogeneous uro-epithelial cell monolayers
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Cytosolic calcium and nuclear calcium-regulated transcription networks in response to ABA and JA
GEO Series GSE109611. Arabidopsis thaliana. 29 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.