Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
13
datasets available to search
ShareScore release 0.9.0
Dataset results
13 results for “cytosolic protein”
A proteome-wide map of chaperone-assisted protein refolding in a cytosol-like milieu
<p>The journey by which proteins navigate their energy landscapes to their native structures is complex, involving (and sometimes requiring) many cellular factors and processes operating in partnership with a given polypeptide chain's intrinsic energy landscape. The cytosolic environment and its complement of chaperones play critical roles in granting many proteins safe passage to their native states; however, it is challenging to interrogate the folding process for large numbers of proteins in a complex background with most biophysical techniques. Hence, most chaperone-assisted protein refolding studies are conducted in defined buffers on single purified clients. Here, we develop a limited-proteolysis mass spectrometry approach paired within an isotope-labeling strategy to globally monitor the structures of refolding <em>E</em>. <em>coli</em> proteins in the cytosolic medium and with the chaperones, GroEL/ES (Hsp60) and DnaK/DnaJ/GrpE (Hsp70/40). GroEL can refold the majority (85%) of the <em>E</em>. <em>coli</em> proteins for which we have data, and is particularly important for restoring acidic proteins and proteins with high molecular weight, trends that come to light because our assay measures the structural outcome of the refolding process itself, rather than binding or aggregation. For the most part, DnaK and GroEL refold a similar set of proteins, supporting the view that despite their vastly different structures, these two chaperones both unfold misfolded states, as one mechanism in common. Finally, we identify a cohort of proteins that are intransigent to being refolded with either chaperone. The data support a model in which chaperone-nonrefolders may fold most efficiently cotranslationally, and then remain kinetically trapped in their native conformations.</p>
A proteome-wide map of chaperone-assisted protein refolding in a cytosol-like milieu
Open the record for dataset details and reuse information.
Cytosolic splice isoform of Hsp70 nucleotide exchange factor Fes1 is required for the degradation of misfolded proteins in yeast
GEO Series GSE78136. Saccharomyces cerevisiae. 18 samples. Type: Expression profiling by high throughput sequencing.
Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response [time course]
GEO Series GSE299855. Saccharomyces cerevisiae. 35 samples. Type: Other.
Cytosolic aggregation of mitochondrial proteins enhances degeneration of cellular homeostasis
GEO Series GSE147284. Saccharomyces cerevisiae. 68 samples. Type: Expression profiling by high throughput sequencing.
Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response [steady state]
GEO Series GSE299862. Saccharomyces cerevisiae. 21 samples. Type: Other.
The cytosolic iron-sulfur cluster assembly protein MMS19 regulates transcriptional gene silencing, DNA repair, and flowering time in Arabidopsis
GEO Series GSE67669. Arabidopsis thaliana. 3 samples. Type: Expression profiling by high throughput sequencing.
Cytosolic Ribosomal Protein Haploinsufficiency affects Mitochondrial Morphology and Respiration
GEO Series GSE280071. Caenorhabditis elegans. 40 samples. Type: Expression profiling by high throughput sequencing; Other.
Cellular response to unfolded proteins in cytosol and nucleus
GEO Series GSE65504. Mus musculus. 15 samples. Type: Expression profiling by high throughput sequencing.
Nuclear and cytosolic J-domain proteins provide synergistic control of Hsf1 at distinct phases of the heat shock response
GEO Series GSE298376. Saccharomyces cerevisiae. 12 samples. Type: Expression profiling by high throughput sequencing.
Plant response to misfolded protein in the cytosol
GEO Series GSE11758. Arabidopsis thaliana. 22 samples. Type: Expression profiling by array.
Retrograde Control of Cytosolic Translation Targets Synthesis of Plastid Proteins and Nuclear Responses for High-Light Acclimation.
GEO Series GSE211494. Arabidopsis thaliana. 12 samples. Type: Expression profiling by high throughput sequencing; Other.
Retrograde Control of Cytosolic Translation Targets Synthesis of Plastid Proteins and Nuclear Responses for High-Light Acclimation
GEO Series GSE211805. Arabidopsis thaliana. 16 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.