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138 results for “dissolved organic carbon”
Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) from river, lagoon, and open ocean sites along the Alaska Beaufort Sea coast, 2018-ongoing
Multiple water types (river, lagoon, ocean) from the North Slope of Alaska and nearshore Beaufort Sea are sampled seasonally by the Beaufort Lagoon Ecosystems LTER (BLE LTER) Core Program to investigate biogeochemical linkages between terrestrial, lagoon, and open ocean ecosystems. Water samples are collected during full ice cover (April), ice break-up (mid-June to early July), and open water (late July and August) periods and analyzed for dissolved organic carbon and total dissolved nitrogen content.
Total dissolved nitrogen (TDN), dissolved organic carbon (DOC), radiocarbon (14C-DOC), and stable carbon (13C-DOC) of surface waters from the Canning River watershed, 2019 and 2021
Sites along the Canning River mainstem and contributing streams near the Kavik River Camp, Alaska, were visited to track changes in stream and river total dissolved nitrogen (TDN) concentration, dissolved organic carbon (DOC) concentration, and the stable carbon (13C) and radiocarbon (14C) isotopic composition of DOC across transitions between the Brooks Range, Brooks foothills, and Arctic Coastal Plain. The dataset also includes water samples collected from lakes, springs, groundwater, and streams and rivers outside the Canning River watershed. Water samples were collected in late April and early August 2019 and in late July and early August 2021. Data include measurements of individual samples for TDN (milligrams nitrogen per liter), DOC (milligrams carbon per liter), carbon-13 of DOC (reported as delta-13C, per mil), carbon-14 of DOC (reported as fraction modern), and analytical error in the fraction modern values. Additional water chemistry data for these samples can be found in Koch et al. (2024). References: Koch, J. C., Connolly, C. T., Repasch, M., Best, H. R., Couvillion, C. S., Hunt, A. (2024). [Dataset] Hydrochemistry and age date tracers from springs, streams, and rivers in the Arctic National Wildlife Refuge, 2019-2022, U.S. Geological Survey data release, https://doi.org/10.5066/P95CXJIT.
Ramped Pyrolysis Oxidation (RPO) coupled radiocarbon (14C-DOC) and stable carbon (13C-DOC), high-resolution molecular composition (FT-ICR MS), and biodegradable dissolved organic carbon (BDOC) of groundwater, river water, and lagoon water in northeast Alaska, 2017
Supra-permafrost groundwater (SPGW), river water, and lagoon water were sampled near Kaktovik, AK to assess the reactivity and origin of dissolved organic matter (DOM) across interconnected hydrologic systems during late summer. Water samples were collected on August 17th 2017 from SPGW along the beach of Jago Lagoon (Jago GW), surface water from the Jago River’s main channel above tidal influence (Jago R), and from the water column of Kaktovik Lagoon at 2–3 m depth (KA LW). Measurements were made from grab samples for river and lagoon water, and from a composite sample for SPGW gathered from 10 individual shoreline locations. Data include dissolved organic carbon concentration (DOC, mg C L-1), Ramped Pyrolysis Oxidation (RPO) derived fraction compositions of 13C-DOC (δ13C ‰), 14C-DOC (in fraction modern), and method/instrumental error in the 14C and 13C results, and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) molecular composition and summarized compound classes. Biodegradable DOC (BDOC) bottle experiments were performed using all three sample types, where DOC concentration was subsequently measured at 2, 7, 14, and 28 days. FT-ICR MS composition was subsequently measured at the 28-day timepoint to track changes in molecular formulae and compound class relative abundance following biodegradation. Data from RPO serial thermal oxidation include temperature and normalized CO2 profiles for each background sample. Thermal-oxidation profiles of CO2 were transformed into non-parametric activation energy (E) distributions using an inverse model. Model output includes C mass of oxidized CO2 (µg C), Tmax (K), Emax (kJ mol-1), Emean (kJ mol-1), Estd (kJ mol-1), and p(0,E)max of user-defined sample fractions. FT-ICR MS results include a summary table of the relative abundance of compound classes (e.g., unsaturated phenolic, polyphenolic, aliphatic, condensed aromatics, peptide-like) and elemental groupings (e.g., CHO-type, CHON-type, CHOS-type, CHON
Correction Factors for Dissolved Organic Carbon Extracted from Soil in New England 2012-2013
Oxidizable dissolved organic carbon (DOC) is regularly measured in environmental samples using a colorimetric method with Mn(III)-pyrophosphate as the oxidizing agent. It is simpler to use and has a much higher throughput than the commonly used dichromate oxidation and combustion methods. Here, we demonstrate that the method often leads to an underestimation or overestimation of the concentration of common organic compounds in solutions. To our knowledge, no published study has taken this fact into account when analyzing DOC data. Hence, we compared Mn(III)-pyrophosphate-based results with measurements performed with a total organic carbon combustion analyzer for samples of organic and mineral soil horizons of two temperate deciduous forests (Harvard Forest, Hubbard Brook), of organic soil horizon of a primary growth hemlock stand (Harvard Forest), and of a peatland (Caribou Bog) located in New England, USA. The Mn(III)-pyrophosphate method consistently underestimated DOC concentration in soil extracts. We present correction factors for the different types of soil studied. By employing correction factors, we find the method can be an inexpensive, accurate, and high throughput tool to measure DOC in environmental samples.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water years 2017 and 2018 (1 Oct 2016 - 30 Sep 2018)
These data were collected by the University of Montana and Montana State University to support the Upper Clark Fork River restoration monitoring project supported by the US NSF Long Term Research in Environmental Biology (LTREB) program. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2017 and 2018 water year (1 Oct 2016 to 30 Sep 2018). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2020 (1 Oct 2019 - 30 Sep 2020)
These data were collected to support monitoring of the Upper Clark Fork River restoration, and data collection was funded by the US NSF Long Term Research in Environmental Biology (LTREB) program and the US NSF EPSCoR funded Montana Consortium for Research on Environmental Water Systems. The LTREB monitoring project consists of monthly or bi-weekly water quality monitoring across a 200-km restoration gradient contaminated by historic mining practices to monitor inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and heavy metal contamination. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2020 water year (1 Oct 2019 to 30 Sep 2020). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Multispectral absorbance and fluorescence analysis of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water years 2017 and 2018 (1 Oct 2016 - 30 Sep 2018)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding likely to be extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are multispectral absorbance and fluorescence analyses of organic carbon dissolved in samples of well-mixed river thalweg water. Data include excitation-emission matrices, absorbance spectroscopy, as well as absorbance and fluorometric summary indices calculated at specific wavelengths of excitation and emission. Data are from the 2017 and 2018 water years (1 Oct 2016 to 30 Sep 2018). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at 13 project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA. These data are a correction of a previously published data product (doi:10.6073/pasta/6ba30f4ebb63175a4399c5d0aa6a8698). Inconsistencies between availability of EEMS data, absorbance data, and fluorometric summary metrics have been corrected. P
Multispectral absorbance and fluorescence analysis of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2019 (1 Oct 2018 - 30 Sep 2019)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding likely to be extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are multispectral absorbance and fluorescence analyses of organic carbon dissolved in samples of well-mixed river thalweg water. Data include excitation-emission matrices, absorbance spectroscopy, as well as absorbance and fluorometric summary indices calculated at specific wavelengths of excitation and emission. Data are from the 2019 water year (1 Oct 2018 to 30 Sep 2019). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at 13 project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2021 (1 Oct 2020 - 30 Sep 2021)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2021 water year (1 Oct 2020 to 30 Sep 2021) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2023 (1 Oct 2022 - 30 Sep 2023)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are total organic carbon combustion analyses (Shimadzu Scientific Instruments) of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2023 water year (1 Oct 2022 to 30 Sep 2023) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2024 (1 Oct 2023 - 30 Sep 2024)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are total organic carbon combustion analyses (Shimadzu Scientific Instruments) of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2024 water year (1 Oct 2023 to 30 Sep 2024) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2022 (1 Oct 2021 - 30 Sep 2022)
The Upper Clark Fork River (UCFR) Long Term Research in Environmental Biology (LTREB) umbrella monitoring project generating these data is conducted separately and complementarily to the 200-million-dollar (USD) superfund project for ecological restoration of the UCFR, associated tributaries, and head water streams including Silver Bow and Warm Springs Creeks. Restoration along the UCFR in western Montana includes removal of metal-laden floodplain soils, lowering of the floodplain to its original elevation, and re-vegetation of over 70 km of the river’s floodplain closest to contaminant sources. The UCFR LTREB project includes bi-weekly water quality monitoring across the first 200 km of the river and its major tributaries along a gradient of heavy metal contamination associated with historic mining. Monitoring includes inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and dissolved and whole-water heavy metal concentrations. The monitoring program began in 2017 with funding extended through 2028. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are primarily Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. A few samples from the final campaign in the dataset were analyzed with a Shimadzu instrument using a similar method. Data are from the 2022 water year (1 Oct 2021 to 30 Sep 2022) from samples collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Concentration of dissolved organic carbon in water samples taken from the Upper Clark Fork River (Montana, USA) during water year 2019 (1 Oct 2018 - 30 Sep 2019)
These data were collected to support monitoring of the Upper Clark Fork River restoration, and data collection was funded by the US NSF Long Term Research in Environmental Biology (LTREB) program and the US NSF EPSCoR funded Montana Consortium for Research on Environmental Water Systems. The LTREB monitoring project consists of monthly or bi-weekly water quality monitoring across a 200-km restoration gradient contaminated by historic mining practices to monitor inorganic phosphorus and nitrogen concentrations, biotic standing stocks, and heavy metal contamination. The original analytical intent for these data was to assess the response of river dissolved organic carbon to the floodplain restoration. Data are Aurora Total Organic Carbon combustion analyses of the concentration of organic carbon dissolved in filtered samples of well-mixed river thalweg water. Data are from the 2019 water year (1 Oct 2018 to 30 Sep 2019). Data were collected on the Upper Clark Fork River (USGS HUC 17010201) at project sites distributed along the river from the vicinity of Anaconda to Missoula, Montana, USA.
Salt River Wetlands denitrification rate, dissimilatory nitrate reduction to ammonium rate, dissolved organic carbon concentration in June 2016 as well as soil porosity and bulk density
Raw and derived data used to calculate denitrification and dissimilatory nitrate to ammonium (DNRA) from push-pull experiments with added isotopically labelled nitrate. Experiments were conducted in 2016 in the Salt River Accidental Wetlands in three different patch types: Unvegetated, dominated by Ludwigia peploides, and dominated by Typha species (T. domingensis and T. latifolia). Data include start and end of incubation concentration of nitrate, ammonium, atom percent 15N in ammonium, dissolved organic carbon, excess mass 29-N2, and excess mass 30-N2. Soil data was collected from the same patch types including soil moisture, porosity, and bulk density.
Spatial and Temporal Patterns in Atmospheric Deposition of Dissolved Organic Carbon
Atmospheric deposition of dissolved organic carbon (DOC) to terrestrial ecosystems is a small, but rarely studied component of the global carbon (C) cycle. Emissions of volatile organic compounds (VOC) and organic particulates are the sources of atmospheric C and deposition represents a major pathway for the removal of organic C from the atmosphere. Here, we evaluate the spatial and temporal patterns of DOC deposition using 70 datasets at least one year in length ranging from 40° south to 66° north latitude. Globally, the median DOC concentration in bulk deposition was 1.7 mg L-1. The DOC concentrations were significantly higher in tropical (< 25°) latitudes compared to temperate (> 25°) latitudes. DOC deposition was significantly higher in the tropics because of both higher DOC concentrations and precipitation. Using the global median or latitudinal specific DOC concentrations leads to a calculated global deposition of 202 or 295 Tg C yr-1 respectively. Many sites exhibited seasonal variability in DOC concentration. At temperate sites, DOC concentrations were higher during the growing season; at tropical sites, DOC concentrations were higher during the dry season. Thirteen of the thirty-four long-term (> 10 years) datasets showed significant declines in DOC concentration over time with the others showing no significant change. Based on the magnitude and timing of the various sources of organic C to the atmosphere, biogenic VOCs likely explain the latitudinal pattern and the seasonal pattern at temperate latitudes while decreases in anthropogenic emissions are the most likely explanation for the declines in DOC concentration.
Hydrochemical Data from a Tropical Andean Glacierized Catchment: δ18O, electrical conductivity, maximum fluorescence intensity, and dissolved organic carbon concentrations from short-term sampling campaigns, Ecuador (2022 and 2024)
Fluorescent dissolved organic matter (FDOM) quality, dissolved organic carbon (DOC) concentration, electrical conductivity (EC), and stable water isotopes (δ¹⁸O and δ2H) were determined in water, snow, and ice samples from a tropical glacierized catchment in the Ecuadorian Andes. The sampling locations were selected to capture the major hydrologic inputs to the main stream channel (glacial melt, tributaries, wetlands, and groundwater springs) and constrain the in-stream spatiotemporal variation in DOM quality and other hydrochemical characteristics. Two sets of high-resolution time series were collected on Oct 13, 2022 and Jun 14, 2024. Time series samples were collected at various upper catchment locations and the outlet simultaneously. DOM quality was characterized via fluorescence spectroscopy and processed using parallel factor analysis (PARAFAC). The DOM quality data are expressed as %FMax values obtained through a 4-component PARAFAC model, where %Fmax 1– 4 are interpreted as terrestrial humic-like, tyrosine-like, tryptophan-like, and microbial humic-like fluorescent components, respectively. DOC concentrations were quantified using high-temperature catalytic combustion, stable water isotopes were analyzed using laser-based spectroscopy, and EC was measured in situ with handheld multiparameter water quality probes.
High-frequency dissolved organic carbon and nitrate from the Oksrukuyik Creek outlet near Toolik Field Station,Alaska, summer 2017-2019
Data file describing high frequency (every ~10 minutes), optial sensor-derived chemistry of river water from Oksukuyik Creek near Toolik Field Station, North Slope of Alaska. Data file includes date, time, dissolved organic carbon (DOC) concentration, and nitrate concentration. Sensors (V2 s::can uv-vis spectrophotometers) were continuously deployed from June through August or September and optically determined nitrate and dissolved organic carbon concentrations.
Model estimates of runoff, dissolved organic carbon, soil temperature and moisture for Elson Lagoon watershed, Alaska, 1981-2020
This dataset contains model estimates of dissolved organic carbon (DOC) yield (mg C/m^2) and runoff (mm), for surface and subsurface flows, soil temperature (degree C), and soil moisture (% of soil volume) for grid cells spanning the Elson Lagoon watershed in northwest Alaska. Daily air temperature, precipitation, and wind speed data from Utqiagvik airport were used for meteorological forcings for the daily simulation by the Permafrost Water Balance Model (PWBM) from 1981 to 2020. The DOC and runoff data files are organized by grid cell and month. The soil temperature and soil moisture files are organized by grid cell and day of year (DOY), and contain values for the first eight model soil layers, with centers of the layers at 1, 3, 8, 13, 23, 33, 45, 55 cm depth. The estimates are most useful for analyses of the dynamics of the watershed’s surface and subsurface runoff and DOC yield. Leachate DOC concentrations can be obtained using the gridded runoff and yield values. A manuscript describing the data and associated analysis has been accepted for publication in Environmental Research Letters (Rawlins et al., 2021).
Quantitative and qualitative aspects of dissolved organic carbon leached from plant biomass in Taylor Slough, Shark River and Florida Bay (FCE) for samples collected in July 2004
Plant biomass was collected from Taylor Slough, Shark River and Florida Bay in Everglades National Park. Samples were taken to the lab and incubated with Milli-Q water in the dark for a period of 36 days. NaN3 was added to half the bottles to test the role of microbial activity on the leaching rates and composition of leachate. Every three days the water was decanted and replaced with fresh Milli-Q water. The decanted samples were filtered and analyzed for DOC concentration, sugar content, and total phenol content.
Dissolved Organic Carbon Stable Isotopes and Lignin Phenols from Everglades National Park (FCE LTER), South Florida, USA, January 2019 - ongoing
Estuarine and coastal waters are home to more than half of the world's population and provide many social and economic benefits to society. Additionally, these ecosystems are sites of intense carbon cycling and are under pressure from changing land uses and climate. DOM is functionally defined as the OM passed through a 0.7 µm borosilicate glass fiber filter (GF/F) and is primarily made up of viruses, bacteria, colloids, lignins, humic substances, organic acids, and other small organic compounds (Repeta, 2015). Approximately 50% of DOM is generally considered to be dissolved organic carbon (DOC) (Stedmon and Nelson, 2015). Thus, DOC can be used as a proxy for quantifying DOM. Whole water samples were collected monthly from three different transects representing marl-dominated (Taylor Slough), peat-dominated (Shark River Slough), and seagrass-dominated (Florida Bay) environments. The water was filtered using 0.7 µm porosity glass fiber filters, then acidified to pH 2 and stored at 4°C until processing occurred. DOC concentration and the stable isotope ratio of DOC (δ13C-DOC) was measured on an OI Analytical TOC analyzer coupled to an Agilent Delta V plus Isotope Ratio Mass Spectrometer using high-temperature combustion (Lalonde et al., 2014). Dissolved vanillyl (vanillin, acetovanillone, vanillin acid), syringyl (syringaldehyde, acetosyringone, syringic acid), cinnamyl (p-coumaric acid, ferulic acid), and 3,5-hydroxybenzoic acid lignin phenols were isolated from ~2 liters of 0.7 µm filtered, acidified (pH 2) water by solid phase extraction (SPE) using 1g PPL cartridges that were pre-conditioned using pH 2 water and methanol. SPE cartridges were extracted using methanol, then dried and redissolved in 2N NaOH. Cupric oxide oxidation and liquid-liquid ethyl acetate extraction were used to extract lignin oxidation products (Hedges and Ertel, 1982, modified by Goñi and Hedges, 1995; Louchouarn et al., 2000; Goñi and Montgomery, 2000; Benner and Kaiser, 2011). Briefly, NaO
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Allen Brain Atlas
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