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6 results for “egg composition”
Diversity and composition of the microbiome associated with eggs of the Southern green stinkbug, Nezara viridula (Hemiptera: Pentatomidae)
<p><strong>Background:</strong> Although microbial communities of insects from larval to adult stage have been increasingly investigated in recent years, little is still known about the diversity and composition of egg-associated microbiomes. In this study, we used high-throughput amplicon sequencing and qPCR to get a better understanding of the microbiome of insect eggs and how they are established using the Southern green stinkbug <em>Nezara viridula </em>(L.) (Hemiptera: Pentatomidae) as study object. First, in order to determine the bacterial community composition, egg masses from two natural populations in Belgium and Italy were examined. Subsequently, microbial community establishment was assessed by studying stinkbug eggs of different ages obtained from laboratory strains (unlaid eggs collected from the ovaries, eggs less than 24 hours old, and eggs collected four days after oviposition). Both the external and internal egg-associated microbiome were analyzed by investigating egg washes and surface-sterilized washed eggs, respectively.</p> <p><strong>Results:</strong> Eggs from the ovaries were completely devoid of bacteria, indicating that egg-associated bacteria were deposited on the eggs during or after oviposition. Bacterial diversity of deposited eggs was very low, with on average 6.1 zOTUs in the external microbiome and 1.2 zOTUs in internal samples of egg masses collected from the field. Bacterial community composition and density did not change significantly over time, suggesting limited bacterial growth. A <em>Pantoea</em>-like symbiont previously found in the midgut of <em>N. viridula</em> was found in every sample and generally occurred at high relative and absolute densities, especially in the internal egg samples. Additionally, some eggs harbored a <em>Sodalis</em> symbiont, which has previously been found in the abdomen of several insects, but so far not in <em>N. viridula</em> populations.</p> <p><strong>Conclusions:</strong> We conclude that the egg-associated bacterial microbiome of <em>N. viridula</em> is species-poor and dominated by a few symbionts, particularly the species-specific obligate <em>Pantoea</em>-like symbiont.</p>
Data from: Experimental increase in temperature affects eggshell thickness, and not egg mass, eggshell spottiness or egg composition in the great tit (Parus major)
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Data from: Effect of composition on shape of bird eggs
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Mother-specific composition of the maternal transcriptome in Zebrafish eggs
GEO Series GSE72839. Danio rerio. 24 samples. Type: Expression profiling by array.
Effects of Increased, Egg-Based Protein Intake on Muscle Composition,
ClinicalTrials.gov study NCT01396915. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Long-Term Consumption Of Poultry Egg Products Provided Stable Growth Demographics, Body Composition, Glucose Control, And Liver Gene Expression In The Zebrafish Danio rerio
GEO Series GSE184565. Danio rerio. 8 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.