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4 results for “electrofishing survey”
Interagency Ecological Program: USFWS Delta Boat Electrofishing Survey, 2018-2025
The United States Fish and Wildlife Service's (USFWS) Delta Boat Electrofishing Survey was designed to estimate spatial and temporal trends in abundance, occupancy, capture probabilities, and related environmental drivers of fish assemblages across nearshore littoral habitats within the Sacramento-San Joaquin River Delta. The USFWS Lodi Field Office, in collaboration with the United States Geological Survey, developed the survey in 2018 to expand fish monitoring and inferences in nearshore habitats not monitored by long-term USFWS beach seine surveys within the San Francisco Estuary. The survey uses a robust statistical framework, including a stratified random sampling design and segmented transects that act as spatial replicates for occupancy modeling. For more information on the Lodi U.S. Fish and Wildlife Office: https://www.fws.gov/lodi/
Interagency Ecological Program: Sacramento-San Joaquin Delta boat electrofishing survey during the critical drought of 2014
The objective of this study was to assess the relative distributions of non-native resident fishes occurring in littoral habitats during the drought year of 2014 within the western and northern portion of California's Sacramento-San Joaquin Delta (Delta), USA. A number of non-native resident fish species (e.g., black bass, Micropterus spp.) in the Delta have undergone remarkable increases in density in the early 2000s and they are potential predators of endangered native fishes such as Chinook Salmon (Oncorhynchus tshawytscha) and Delta Smelt (Hypomesus transpacificus). The Delta makes up the tidal freshwater portion of the San Francisco Estuary in California; however, the critically dry year of 2014 caused a considerable intrusion of salinity field within this region and may have caused a shift in the resident fish community. To evaluate the response of non-native resident fishes to this salinity intrusion, a boat electrofishing survey was conducted in November of 2014 in the western and northern part of the Delta by the United States Fish and Wildlife Service, Lodi, California Office as part of the Interagency Ecological Program for the San Francisco Estuary. Replicate sampling was done for each site in this study in order to account for false zeroes through the estimation of capture probability.
Multi-species models reveal that eDNA metabarcoding is more sensitive than backpack electrofishing for conducting fish surveys in freshwater streams
Environmental DNA (eDNA) sampling can provide accurate, cost-effective, landscape-level data on species distributions. Previous studies have compared the sensitivity of eDNA sampling to traditional sampling methods for single species, but similar comparative studies on multi-species eDNA metabarcoding are rare. Using hierarchical species occupancy-detection models, we examined whether key choices associated with eDNA metabarcoding (primer selection, low-abundance read filtering, and the number of positive water samples used to classify a species as present at a site) affect the sensitivity of metabarcoding, relative to backpack electrofishing for fish in freshwater streams. Under all scenarios (teleostei and vertebrate primers; 0%, 0.1% and 1% read filtering thresholds; 1 or 2 positive samples required to classify species as present), we found that eDNA metabarcoding is, on average, more sensitive than electrofishing. Combining vertebrate and teleostei markers resulted in higher detection probabilities relative to the use of either marker in isolation. Increasing the threshold used to filter low abundance reads decreased species detection probabilities but did not change our overall finding that eDNA metabarcoding was more sensitive than electrofishing. Using a threshold of two positive water samples (out of 5) to classify a species as present typically had negligible effects on detection probabilities compared to using one positive water sample. Our findings demonstrate that eDNA metabarcoding is generally more sensitive than electrofishing for conducting fish surveys in freshwater streams, and that this outcome is not sensitive to methodological decisions associated with metabarcoding.
Multi-species models reveal that eDNA metabarcoding is more sensitive than backpack electrofishing for conducting fish surveys in freshwater streams
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