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7 results for “ergot alkaloid”
Virulence of three Aspergillus species to the model insect Galleria mellonella and the contribution of ergot alkaloids to the pathogenic potential of Aspergillus leporis
<p>Opportunistically pathogenic fungi have varying potential to cause disease in animals. Factors contributing to their virulence include specialized metabolites, which is some cases evolved in contexts unrelated to pathogenesis. Specialized metabolites that increase fungal virulence in the model insect <em>Galleria mellonella</em> include the ergot alkaloids fumigaclavine C in <em>Aspergillus fumigatus</em> (syn. <em>Neosartorya fumigata</em>) and lysergic acid α-hydroxyethylamide (LAH) in the entomopathogen <em>Metarhizium brunneum</em>. Three species of <em>Aspergillus</em> recently found to accumulate high concentrations of LAH were investigated for their pathogenic potential in <em>G. mellonella</em>. A<em>spergillus leporis</em> was most virulent, <em>A. hancockii </em>was intermediate, and <em>A. homomorphus</em> had very little pathogenic potential. <em>Aspergillus leporis</em> and <em>A. hancockii </em>emerged from and sporulated on dead insects, thus completing their asexual life cycles. Inoculation by injection resulted in more lethal infections than did topical inoculation, indicating <em>A. leporis</em> and <em>A. hancockii</em> were pre-adapted for insect pathogenesis but lacked an effective means to breach the insect's cuticle. All three species accumulated LAH in infected insects, with <em>A. leporis</em> accumulating the most. Concentrations of LAH in <em>A. leporis</em> were similar to those observed in the entomopathogen <em>M. brunneum</em>. LAH was eliminated from <em>A. leporis</em> through a CRISPR/Cas9-based gene knockout, and the resulting strain had reduced virulence to <em>G. mellonella</em>. The data indicate <em>A. leporis</em> and <em>A. hancockii</em> have considerable pathogenic potential and that LAH increases the virulence of <em>A. leporis</em>.</p>
Analyses of ergot alkaloid production in Aspergillus leporis and an easD knockout and sequences for phylogenetic analyses of rugulovasine-associated genes
<p>Ergot alkaloids are fungal specialized metabolites that are important in agriculture and serve as sources of several pharmaceuticals. <em>Aspergillus</em> <em>leporis</em> is a soil saprotroph that possesses two ergot alkaloid biosynthetic gene clusters encoding lysergic acid amide production. We identified two additional, partial biosynthetic gene clusters within the <em>A</em>. <em>leporis</em> genome containing some of the ergot alkaloid synthesis (<em>eas</em>) genes required to make two groups of clavine ergot alkaloids, fumigaclavines and rugulovasines. Clavines possess unique biological properties compared to lysergic acid derivatives. Bioinformatic analyses indicated the fumigaclavine cluster contained functional copies of <em>easA</em>, <em>easG</em>, <em>easD</em>, <em>easM</em>, and <em>easN</em>. Genes resembling <em>easQ</em> and <em>easH</em>, which are required for rugulovasine production, were identified in a separate gene cluster. The pathways encoded by these partial, or satellite, clusters would require intermediates from the previously described lysergic acid amide pathway to synthesize a product. Chemical analyses of <em>A. leporis</em> cultures revealed the presence of fumigaclavine A. Rugulovasine was only detected in a single sample, however, prompting a heterologous expression approach to confirm functionality of <em>easQ</em> and <em>easH</em>. An <em>easA</em> knockout strain of <em>Metarhizium</em> <em>brunneum</em>, which accumulates the rugulovasine precursor chanoclavine-I aldehyde, was chosen as expression host. Strains of <em>M. brunneum</em> expressing <em>easQ</em> and <em>easH</em> from <em>A. leporis</em> accumulated rugulovasine as demonstrated through mass spectrometry analysis. These data indicate that <em>A. leporis</em> is exceptional among fungi in having the capacity to synthesize products from three branches of the ergot alkaloid pathway and for utilizing an unusual satellite cluster approach to achieve that outcome.</p>
Analyses of ergot alkaloid production in Aspergillus leporis and an easD knockout and sequences for phylogenetic analyses of rugulovasine-associated genes
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Virulence of three Aspergillus species to the model insect Galleria mellonella and the contribution of ergot alkaloids to the pathogenic potential of Aspergillus leporis
Open the record for dataset details and reuse information.
Mid-and Long-term Efficacy of Ergot Alkaloids in the Treatment of Salivation in Patients With Parkinson's Disease.
ClinicalTrials.gov study NCT06035913. IPD Sharing: NO. Countries: 1. Publications: 0.
Ergot alkaloid induced changes during late gestation in ewes
GEO Series GSE195593. Ovis aries. 15 samples. Type: Expression profiling by high throughput sequencing.
Expression Profiling of Heat Stress Effects on Mice Fed Ergot Alkaloids.
GEO Series GSE5642. Mus musculus; Rattus norvegicus. 29 samples. Type: Expression profiling by array.
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