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280 results for “erythropoietin”
Erythropoietin directly remodels the clonal composition of murine hematopoietic multipotent progenitor cells
<p>## In version 1 of the repository the scRNAseq data was corrupted -- in version 2 this has been corrected and the bam files of both scRNAseq runs have been uploaded ##</p> <p>This dataset consists of the raw sequencing files of 9 independent barcoding experiments and of one 10X Genomics scRNAseq experiment. These are the source files for the main figures of the associated publication.</p> <p>HSPCs (C-Kit+ Sca1+ CD150+ Flt3-) or MPP2 (C-Kit+ Sca1+ Flt3- CD150+ CD48+) were isolated form mouse bone marrow through flushing, MACS-enrichment and sorting. The cells were barcoded by spin infection for 6h, and cultured in StemSpanMedium SFEM with 50 ng/ml mSCF (STEMCELL Technologies) for 16h with or without human recombinant EPO (Eprex, erythropoietin alpha, Janssen) at 1000 ng/ml or 160 ng/ml. At this stage scRNAseq was perfromed on the 10X Chromium platfom (10X Genomics), or cells were transplanted by tail vein injection into 6Gy irradiated recipient mice. When appropirate an additional injection of EPO 133ug/kg was given at the moment of transplantation. Different mature hematopoietic cells were isolated form the transplanted mice after 4 weeks or 16 weeks.</p> <p>For barcoding experiments, a three-step PCR was performed to amplify barcode sequences, to add Read1 and Read2 Illumina sequencing adapters, P5 and P3 flow cell attachment sites as well as plate and sample indices. Libraries were sequenced on an Illumina HiSeq SR65 with 10% of PhiX spike-in. For scRNAseq experiment, libraries were made using the Chromium SIngle Cell 3' v2 kit and sequencing was performed on a HiSeq PE26-98.</p> <p>The repository encompasses the following datasets:</p> <ul> <li>A1006.tar.gz -- AE05Low -- HSPCs EPO 160 ng/ml + injection 4 weeks -- main figure 2</li> <li>A1007.tar.gz -- AE05High -- HSPCs EPO 1000 ng/ml + injection 4 weeks -- main figure 2</li> <li>A984.tar.gz -- AE03Low -- HSPCs EPO 160 ng/ml 4 weeks -- main figure 2 and HSPC part of main figure 4</li> <li>A1008.tar.gz -- AE03High -- HSPCs EPO 1000 ng/ml 4 weeks -- main figure 1, 2 and DC part of main figure 3</li> <li>A1012.tar.gz -- AE04Low -- HSPCs EPO 160 ng/ml 4 months -- main figure 8</li> <li>A1013.tar.gz -- AE04High -- HSPCs EPO 1000 ng/ml 4 months -- main figure 8</li> <li>A1105.tar.gz -- AE07part1 -- HSPCs EPO 1000 ng/ml 4 weeks part 1 -- MkP part of main figure 3</li> <li>A1107.tar.gz -- AE07part2 -- HSPCs EPO 1000 ng/ml 4 weeks part 2 -- MkP part of main figure 3</li> <li>A1166.tar.gz -- AE13part1 -- HSPCs EPO 1000 ng/ml 4 weeks part 1 -- HSPC part of main figure 4</li> <li>A1166.tar.gz -- AE13part2 -- HSPCs EPO 1000 ng/ml 4 weeks part 2 -- HSPC part of main figure 4</li> <li>D757_3697.tar.gz -- LP26MPP2 -- MPP2 EPO 1000 ng/ml 4 weeks -- main figure 7</li> </ul> <p>In version two of the repository the scRNAseq data is changed into:</p> <ul> <li>possorted_genome_bam_T1.bam -- 10X of EPO exposed (T1) HSPCs -- main figure 5 and 6</li> <li>possorted_genome_bam_T2.bam -- 10X of control (T2) HSPCs -- main figure 5 and 6</li> </ul> <p>The folder for each barcode experiment encompasses fastq files for each sample index used in the experiment. These have to be further de-multiplexed by plate index and barcode reads have to be called. These, and all subsequent processing steps till the making of the main figures are described in the github folder accompanying the associated publication.</p> <p> </p> <p> </p> <p> </p> <p> </p>
Distal and proximal hypoxia response elements cooperate to regulate organ-specific erythropoietin gene expression
<p>While it is well-established that distal hypoxia response elements (HREs) regulate hypoxia-inducible factor (HIF) target genes such as erythropoietin (Epo), an interplay between multiple distal and proximal (promoter) HREs has not been described so far. Hepatic Epo expression is regulated by a HRE located downstream of the <i>EPO</i> gene, but this 3' HRE is dispensable for renal <i>EPO</i> gene expression. We previously identified a 5' HRE and could show that both HREs direct exogenous reporter gene expression. Here, we show that whereas in hepatic cells the 3' but not the 5' HRE is required, in neuronal cells both the 5' and 3' HREs contribute to endogenous Epo induction. Moreover, two novel putative HREs were identified in the <i>EPO</i> promoter. In hepatoma cells HIF interacted mainly with the distal 3' HRE, but in neuronal cells HIF most strongly bound the promoter, to a lesser extent the 3' HRE, and not at all the 5' HRE. Interestingly, mutation of either of the two distal HREs abrogated HIF binding to the 3' and promoter HREs. These results suggest that a canonical functional HRE can recruit multiple, not necessarily HIF, transcription factors to mediate HIF binding to different distant HREs in an organ-specific manner.</p>
Erythropoietin restrains the inhibitory potential of interneurons in the mouse hippocampus: electrophysiology data
<p>This publication contains the recording data of the electrophysiology section of the manuscript entitled<strong> Erythropoietin restrains the inhibitory potential of interneurons in the mouse hippocampus</strong>. Files are organized as follows:</p> <ul> <li>Raw_LFP.zip contains the raw local field potential (LFP) recordings of mice's dorsal hippocampus (CA1), under head-fix recording set up using Open Ephys GUI.</li> <li>README.txt file contains de information about the codification data of mice.</li> </ul> <p>Matlab and R scripts used for data processing are available upon request</p>
High-dose Erythropoietin for Asphyxia and Encephalopathy
ClinicalTrials.gov study NCT02811263. IPD Sharing: YES. Countries: 1. Publications: 10.
Effects of Recombinant Human Erythropoietin on Platelet Function in Patients With Acute Myocardial Infarction
ClinicalTrials.gov study NCT00367991. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Erythropoietin in Hemolytic Uremic Syndrome
ClinicalTrials.gov study NCT03776851. IPD Sharing: YES. Countries: 1. Publications: 9.
A Study to Investigate the Effect of Roxadustat Versus Recombinant Human Erythropoietin (rHuEPO) on Oral Iron Absorption in Chinese Patients With Anemia of Chronic Kidney Disease (CKD)
ClinicalTrials.gov study NCT04655027. IPD Sharing: YES. Countries: 1. Publications: 1.
Azacitidine and Erythropoietin Versus Azacitidine Alone for Patients With Low-Risk Myelodysplastic Syndromes
ClinicalTrials.gov study NCT00379912. IPD Sharing: NO. Countries: 1. Publications: 1.
Comparing Efficacy and Safety of CinnaGen Beta Erythropoietin (CinnaPoietin®) Versus Eprex® on the Treatment of Anemia in ESRD Hemodialysis Patients
ClinicalTrials.gov study NCT03408639. IPD Sharing: NO. Countries: 1. Publications: 37.
Erythropoietin to Enhance Recovery of Erectile Function in Men Following Radical Prostatectomy
ClinicalTrials.gov study NCT00737893. IPD Sharing: NO. Countries: 1. Publications: 5.
Neonatal Erythropoietin And Therapeutic Hypothermia Outcomes in Newborn Brain Injury (NEATO)
ClinicalTrials.gov study NCT01913340. IPD Sharing: Not stated. Countries: 1. Publications: 7.
Erythropoietin and Pediatric Cardiac Surgery
ClinicalTrials.gov study NCT00451698. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Impact of Erythropoietin on Hematological Adaptations and Physical Performance
ClinicalTrials.gov study NCT05078138. IPD Sharing: NO. Countries: 1. Publications: 0.
A Phase 3b Study of Erythropoietin Drugs Using a Specified Dosing Algorithm in Patients With Chronic Kidney Disease on Hemodialysis
ClinicalTrials.gov study NCT02504294. IPD Sharing: Not stated. Countries: 2. Publications: 2.
Effects of Erythropoietin on Cerebral Vascular Dysfunction and Anemia in Traumatic Brain Injury
ClinicalTrials.gov study NCT00313716. IPD Sharing: Not stated. Countries: 1. Publications: 5.
Effect of Erythropoietin (EPO) in Kidney After Cardiac Surgery
ClinicalTrials.gov study NCT00654992. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Erythropoietin for Neonatal Encephalopathy in LMIC (EMBRACE Trial)
ClinicalTrials.gov study NCT05395195. IPD Sharing: YES. Countries: 3. Publications: 3.
Brain Imaging and Developmental Follow up of Infants Treated With Erythropoietin
ClinicalTrials.gov study NCT01207778. IPD Sharing: UNDECIDED. Countries: 1. Publications: 2.
Erythropoietin to Prevent Unnecessary Transfusions In Patients With Cyanotic CHD - A Prospective Control Trial
ClinicalTrials.gov study NCT02564796. IPD Sharing: NO. Countries: 1. Publications: 4.
The Prevention of Erythropoietin on Cardiac Surgery-associated Acute Kidney Injury
ClinicalTrials.gov study NCT03007537. IPD Sharing: NO. Countries: 1. Publications: 10.
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