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121 results for “exocrine”
Data for: 3D in vitro modeling of the exocrine pancreatic unit using tomographic volumetric bioprinting
<p><strong>Abstract</strong></p> <div> <div> <p><span><span>Pancreatic ductal adenocarcinoma (PDAC) is the most frequent type of pancreatic cancer, one of the leading causes of cancer-related deaths worldwide. The first lesions associated with PDAC occur within the functional units of exocrine pancreas</span><span>. T</span><span>he crosstalk between PDAC cells and stromal cells plays a key role in tumor progression.</span><span> Thus,</span> <span>i</span></span><span><span>n vitro</span></span><span><span>, fully human models of the pancreatic cancer microenvironment are needed to foster the development of new, more effective therapies</span><span>.</span> <span>However,</span><span> it is challenging to make these models anatomically and functionally relevant. Here, we used tomographic volumetric bioprinting, a novel method to fabricate </span><span>three-dimensional </span><span>cell-laden constructs</span><span>,</span><span> to produce a </span><span>portion</span><span> of the </span><span>complex convoluted </span><span>exocrine pancreas</span> </span><span><span>in vitro</span></span><span><span>.</span><span> Human fibroblast-laden gelatin methacrylate-based pancreatic models were processed to reassemble the </span><span>tubuloacinar</span><span> structures of the exocrine pancreas and, then human pancreatic ductal epithelial (HPDE) cells overexpressing the KRAS oncogene (HPDE-KRAS) were seeded in the acinar lumen to reproduce the pathological exocrine pancreatic tissue. The growth and organization of HPDE cells within the structure was evaluated and the formation of a thin epithelium which covered the acini inner surfaces in a physiological way inside the 3D model was</span> <span>successfully</span> <span>demonstrated</span><span>. Interestingly, immunofluorescence assays revealed a significantly higher expressions of alpha smooth muscle </span><span>actin</span><span> (α-SMA) vs. </span><span>actin</span><span> in the fibroblasts co-cultured with cancerous than with wild-type HPDE cells. Moreover, α-SMA expression increased with time, and it was found to be higher in fibroblasts that laid closer to HPDE cells than in those </span><span>laying </span><span>deeper into the model. Increased levels of interleukin (IL)-6 were also quantified in supernatants from co-cultures of stromal and HPDE-KRAS cells. These findings correlate with inflamed tumor-associated fibroblast behavior, thus being relevant biomarkers to </span><span>monitor</span><span> the early progression of the disease and to target drug efficacy. </span></span><span> </span></p> </div> <div> <p><span><span>To our knowledge, this is the first</span> <span>demonstration of a </span><span>3D </span><span>bioprinted</span> <span>portion</span><span> of </span><span>pancreas that</span> <span>rec</span><span>apit</span><span>ulates</span> <span>its</span> <span>true 3-dimensional </span><span>microanatomy</span><span>,</span><span> and which shows </span><span>tumor triggered </span><span>inflammation</span><span>. </span></span><span> </span></p> </div> </div> <p> </p> <p><strong>Contents</strong></p> <p>This repository contains the raw data, materials list, protocols, and code necessary to reproduce the work in the namesake preprint.</p> <p> </p>
Additional data for publication: Simple protocol for combined extraction of exocrine secretions and RNA in small arthropods.
<p>Additional data and results are given in this repository. It contains the trimmed reads (fastp; raw reads also on SRA accession numbers SRR29851544-SRR29851549, Bioproject PRJNA1136254), full busco reports for individual transcriptomes, assembly of all six RNAseqs together (transcriptome as base for differential expression analysis) and results of salmon.</p>
Fig. 4 in Hitherto undescribed interommatidial exocrine glands in Chilopoda
Fig. 4. Transmission electron micrographs (TEM), showing fine structural organisation of the interommatidial exocrine gland within the lateral compound eye of Lithobius forficatus. A. Transverse section through proximal region of one secretory gland cell containing typical glandular organelles. The gland cell lobe is surrounded by dense layers of covering and proximal (basal) retinula cells. B. Transverse section through distal region of two neighbouring, unequally sized secretory gland cells; the upper one being penetrated by a small proximal process of the canal cell with the cuticular gland ductule. C. Oblique-transverse section through a triangular interstitium in distal eye region. Two neighbouring, but not yet fused canal cells fill the interommatidial space and are surrounded by covering cells and distal retinula cells. D. Transverse section through middle part of one canal cell containing axially placed cuticular gland ductule. ax = nerve bundle, cc = canal cell, co = cornea, coc = covering (interommatidial pigment) cells, dic = dictyosome, du = cuticular gland ductule, gc = secretory gland cell, mt = mitochondrium, nu = nucleus, pro = cuticular projections, rc = retinula cell, rER = rough endoplasmatic reticulum, sv = secretory vesicle.
Fig. 2 in Hitherto undescribed interommatidial exocrine glands in Chilopoda
Fig. 2. Scanning electron micrographs (SEM) showing the outer morphology of the compound eyes of Scutigera coleoptrata and Lithobius forficatus. A. Lateral view of right compound eye of S. coleoptrata, reproducing fairly dense hexagonal packing of the facets (chosen counting regions marked). B. Dorsal view of one triangular interommatidial space, housing one gland pore opening (S. coleoptrata). C. Lateral view of the left eye of L. forficatus representing a lateral field of 25 condensed, sometimes hexagonally arranged, ommatidia. D. Antero-lateral view of two interommatidial gland pore openings on the corneal surface of the left eye of L. forficatus. an = anterior eye region, ap = apical eye region, do = dorsal, gp = interommatidial gland pore, ld = latero-dorsal eye region, lv = latero-ventral eye region, om = ommatidium, po = posterior eye region, tö = Tömösváry Organ.
Fig. 1 in Hitherto undescribed interommatidial exocrine glands in Chilopoda
Fig. 1. Semi-schematic reconstruction of two different types of scutigeromorph and lithobiomorph interommatidial exocrine gland. A. A longitudinal section through one interommatidial exocrine gland present within the compound eye of Scutigera coleoptrata. B. The probably more advanced lithobiomorph type, where up to three glandular modules are fused to one single interommatidial exocrine gland (Lithobius forficatus, cut-away view). cc = canal cell, co = cornea, du = cuticular gland ductule, gc = secretory gland cell, igc = intermediary gland cell, ipc = interommatidial pigment cell (covering cell), om = ommatidium.
Proton Pump Inhibitors (PPI) and Fat Absorption in Cystic Fibrosis (CF) and Exocrine Pancreatic Insufficiency (EPI)
ClinicalTrials.gov study NCT03551691. IPD Sharing: YES. Countries: 1. Publications: 21.
Study Investigating a Delayed-Release Pancrelipase in Patients With Exocrine Pancreatic Insufficiency Due to Cystic Fibrosis
ClinicalTrials.gov study NCT00510484. IPD Sharing: Not stated. Countries: 5. Publications: 1.
PERT for Treatment of Exocrine Pancreatic Insufficiency in Patients With Unresectable Pancreatic Cancer
ClinicalTrials.gov study NCT02985801. IPD Sharing: NO. Countries: 1. Publications: 19.
Evaluation of Safety and Tolerability of Creon Micro in Children Younger Than Four Years With Pancreatic Exocrine Insufficiency
ClinicalTrials.gov study NCT01747330. IPD Sharing: Not stated. Countries: 1. Publications: 1.
A Double-blind, Randomized, Multicenter, Cross-over Study to Compare the Effect of Creon N and Creon® on Fat Digestion in Subjects ≥ 12 Years of Age With Pancreatic Exocrine Insufficiency Due to Cysti
ClinicalTrials.gov study NCT02137382. IPD Sharing: Not stated. Countries: 2. Publications: 1.
A Study to Investigate How Common Pancreatic Exocrine Insufficiency (PEI) is in Patients With Type 2 Diabetes and Also to Investigate the Uptake of a Single Dose of EPANOVA® or OMACOR® in Patients Wit
ClinicalTrials.gov study NCT02370537. IPD Sharing: Not stated. Countries: 6. Publications: 1.
Study Investigating a Delayed-Release Pancrelipase in Patients With Pancreatic Exocrine Insufficiency Due to Cystic Fibrosis
ClinicalTrials.gov study NCT00775528. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Study Investigating a Delayed-Release Pancrelipase in Patients With Pancreatic Exocrine Insufficiency (PEI) Due to Cystic Fibrosis (CF)
ClinicalTrials.gov study NCT00690820. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Study to Evaluate the Safety and Efficacy of EUR-1008 (APT-1008) Pancreatic Enzyme Product in Participants With Cystic Fibrosis and Exocrine Pancreatic Insufficiency
ClinicalTrials.gov study NCT00297167. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation
<p><em><strong>Supplemental Fig. S1 </strong>(related to Fig. 2)</em>. Mapping and detection of sensory neurons in human pancreatic duct lesion formation.</p> <p><strong><em>Supplemental Table S1.</em></strong> Summary of color codes presented in illustrations.</p> <p><em><strong>Supplemental Table S2. </strong></em>Summary of primary and secondary antibodies used in illustrations.</p> <p> </p> <p><strong>Supplemental Video Legends</strong></p> <p><em><strong>Supplemental Video S1</strong> (related to Fig. 2) </em></p> <p><strong>Human pancreatic substance P<sup>+</sup> sensory (afferent) nerves. </strong></p> <p><strong><em>Left</em> (still image): projection of pancreatic innervation at the acinar-islet boundary. </strong>Blue: glucagon (islet). Green: neuroendocrine marker PGP9.5 (nerve and islet). Red: substance P (SP, sensory nerve). Sensory nerves appear as yellow fibers (overlap of red SP and green PGP9.5 signals). Broken arrow: enlarged area (right panel).</p> <p><strong><em>Right</em>: in-depth recording of peri-ductal sensory nerves. </strong>Sensory nerves (yellow) are around the islet but not penetrating into the core. White: nuclei. Note that a portion of islet cells are SP dim. The still image and recording were derived from a normal human pancreas (female/age, 51 years/BMI, 20).</p> <p> </p> <p><em><strong>Supplemental Video S2</strong> (related to Fig. 2 and Supplemental Fig. S1)</em><em> </em></p> <p><strong>Mapping and detection of immunoreactive substance P<sup>+</sup> neuron in duct lesion formation. </strong></p> <p><strong><em>Left</em> (still image): tissue map of human pancreas distal to ductal adenocarcinoma. </strong>Acinar atrophy is apparent in this condition. Markers: nuclei, white; PGP9.5, green; substance P (SP), red. Broken arrow: intra-pancreatic ganglion (right panel).</p> <p><strong><em>Right</em>: in-depth recording of immunoreactive SP<sup>+</sup> neuron in ganglion. </strong>The nucleus (00:04) and the SP<sup>+</sup> varicosities are prominently seen. The latter contacts the surrounding SP<sup>-</sup> neurons. The still image and recording were derived from an area 5-cm distal to the pancreatic ductal adenocarcinoma (male/age, 77 years/staging, T1N0).</p> <p> </p> <p><em><strong>Supplemental Video S3</strong> (related to Fig. 3) </em></p> <p><strong>Mouse pancreatic substance P<sup>+</sup> sensory (afferent) nerves. </strong></p> <p><strong><em>Left</em> (still image): projection of pancreatic sensory innervation. </strong>In mice, the SP<sup>+</sup> sensory nerves follow the arteriole to the islet mantle and penetrating into the core (enlarged in right panel). Markers: blood vessels, blue; PGP9.5, green; substance P (SP), red.</p> <p><strong><em>Right</em>: in-depth recording of sensory innervation of islet. </strong>Unlike the human pancreatic sensory innervation, the mouse SP<sup>+</sup> sensory nerves (varicosities) are inside the islet and associate with the peri-islet ganglion. White: nuclear staining and tissue autofluorescence. The still image and recording were derived from a B6 mouse pancreas (male/age, 12 weeks).</p>
Exocrine Pancreatic Insufficiency After Acute Pancreatitis and Pancreatic Enzyme Replacement Therapy
ClinicalTrials.gov study NCT05480241. IPD Sharing: NO. Countries: 1. Publications: 28.
Investigation on the Current Situation and Risk Factors Analysis of Exocrine Insufficiency in Chronic Pancreatitis
ClinicalTrials.gov study NCT06946108. IPD Sharing: NO. Countries: 1. Publications: 2.
Post Acute Pancreatitis Pancreatic Exocrine Insufficiency
ClinicalTrials.gov study NCT03063398. IPD Sharing: UNDECIDED. Countries: 1. Publications: 4.
Reorganization of the Healthcare System During COVID-19 Pandemic: Impact on Management of Patients With Exocrine Pancreatic Cancer
ClinicalTrials.gov study NCT04406571. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Role of Pancreatic Exocrine Secretion in Weight Gain After Pancreas Transplantation
ClinicalTrials.gov study NCT04690738. IPD Sharing: NO. Countries: 1. Publications: 5.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.