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18 results for “flagella”
Distinct States in an Active Liquid Crystal of Flagella and Kinesin-Powered Microtubules under Varying Kinesin Concentrations or Active Stress
<p>At a constant nematic elasticity (flagella concentration) and confinement (H=100μm), higher KSA (120nM) or motor concentrations induce robust active stress that disrupts nematic alignment, resulting in chaotic behavior. Conversely, lower KSA (<60nM) concentrations fail to overcome the nematic elastic background's alignment, leading to a unique state where active microtubule bundles phase-separate into lanes within a uniform flagella background. The confocal micrograph reveals this distinct phenomenon, showing uniform flagella distribution alongside microtubule segregation into lane-like structures within the channel's limited confinement.</p>
Distinct States in an Active Liquid Crystal of Flagella and Kinesin-Powered Microtubules under Varying Confinement
<p>At significant confinement levels, the potent active stress disrupts the alignment of the flagella, leading to a turbulent state in the composite liquid crystal. Conversely, minimal confinement enables the dominant nematic alignment to counteract the active stress, inducing the phase separation of microtubules within a uniform flagella background. The confocal micrograph unveils uniform flagella distribution across the medium, while the microtubules segregate into a distinct lane-like structure within the channel's narrowest confinement.</p>
Figure 105-120. Notaepytus spp., male genital flagella. 105 in Pleasing fungus beetles of the West Indies (Coleoptera: Erotylidae: Erotylinae)
Figure 105-120. Notaepytus spp., male genital flagella. 105) N. flavitarsis. 106) N. tarsatus. 107) N. modestus. 108) N. elateroides. 109) N. cyanoros. 110) N. cyclosignatus. 111) N. inversus. 112) N. neibaensis. 113) N. haitensis.114) N. ignotensis. 115) N. fulvitarsis (DR: Hato Mayor). 116) N. fulvitarsis (DR: La Vega). 117) N. fulvitarsis (DR: Barahona). 118) N. decoregens. 119) N. baorucoensis. 120) N. lavegaensis. All reproduced to same scale, scale line = 1 mm.
Data from: High-speed motility originates from cooperatively pushing and pulling flagella bundles in bilophotrichous bacteria
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Coordination of two opposite flagella allows high-speed swimming and active turning of individual zoospores
<p>This contains the datasets of zoospores swimming in water at global scale and during a turning event. We also include the Matlab files for computing the data of global scale data and the simulation for zoospore spreading.</p>
Atypical flagella assembly and haploid genome coiling during male gamete formation in Plasmodium
<p>Movies of Plasmodium berghei SBF-SEM and serial tomogram datasets including 3D models used in publication 'Atypical flagella assembly and haploid genome coiling during male gamete formation in <em>Plasmodium</em>. Molly Hair, Flavia Moreira-Leite, David Ferguson, Mohammed Zeeshan, Rita Tewari, Sue Vaughan. bioRxiv 2023.05.17.540968; doi: https://doi.org/10.1101/2023.05.17.540968'</p> <p><strong>Movie 1.</strong> Serial block face scanning electron microscopy datasets used in this work. The section thickness (Z resolution) is 100 nm, and each dataset consist of 197 images (pixel dimension and X-Y resolution). </p> <p><strong>Movie 2.</strong> SBF-SEM data and segmentation of a single microgametocyte post-activation showing 8 axonemes coiling around the nucleus in two orientations. Organelles are modelled on the following colours: plasma membrane (white), nucleus (blue), basal body (red), axonemes (yellow/blue – two colours to highlight different directions of coiling). </p> <p><strong>Movie 3.</strong> SBF-SEM imaging and 3D model of a microgamete cell undergoing exflagellation. Structures are modelled on the following colours: plasma membrane (white), nucleus (blue), basal body (red), flagella (pink). </p> <p><strong>Movie 4.</strong> Serial tomogram of a microgamete highlighting the coiling of the nucleus around the axoneme. </p> <p><strong>Movie 5.</strong> Serial tomogram of a microgametocyte showing a nuclear projection surrounding the axoneme, with the 3D reconstruction of the nuceloplasm (cyan), which forms a coil within the nuclear projection. </p>
Data from: Dynamic curvature regulation accounts for the symmetric and asymmetric beats of Chlamydomonas flagella
Cilia and flagella are model systems for studying how mechanical forces control morphology. The periodic bending motion of cilia and flagella is thought to arise from mechanical feedback: dynein motors generate sliding forces that bend the flagellum, and bending leads to deformations and stresses, which feed back and regulate the motors. Three alternative feedback mechanisms have been proposed: regulation by the sliding forces, regulation by the curvature of the flagellum, and regulation by the normal forces that deform the cross-section of the flagellum. In this work, we combined theoretical and experimental approaches to show that the curvature control mechanism is the one that accords best with the bending waveforms of Chlamydomonas flagella. We make the surprising prediction that the motors respond to the time derivative of curvature, rather than curvature itself, hinting at an adaptation mechanism controlling the flagellar beat.
Data from: Acclimation temperature changes spermatozoa flagella length relative to head size in brown trout
Temperature is a ubiquitous environmental factor affecting physiological processes of ectotherms. Due to the effects of climate change on global air and water temperatures, predicting the impacts of changes in environmental thermal conditions on ecosystems is becoming increasingly important. This is especially crucial for migratory fish, such as the ecologically and economically vital salmonids, because their complex life histories make them particularly vulnerable. Here, we addressed the question whether temperature affects the morphology of brown trout, Salmo trutta L. spermatozoa. The fertilising ability of spermatozoa is commonly attributed to their morphological dimensions, thus implying direct impacts on the reproductive success of the male producing the cells. We show that absolute lengths of spermatozoa are not affected by temperature, but spermatozoa from warm acclimated S. trutta males have longer flagella relative to their head size compared to their cold acclimated counterparts. This did not directly affect sperm swimming speed, although spermatozoa from warm acclimated males may have experienced a hydrodynamic advantage at warmer temperatures, as suggested by our calculations of drag based on head size and sperm swimming speed. The results presented here highlight the importance of increasing our knowledge of the effects of temperature on all aspects of salmonid reproduction in order to secure their continued abundance.
Data from: Dynamic curvature regulation accounts for the symmetric and asymmetric beats of Chlamydomonas flagella
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Data from: Acclimation temperature changes spermatozoa flagella length relative to head size in brown trout
Open the record for dataset details and reuse information.
Three paralogous small proteins required for flagella-dependent motility in Salmonella
GEO Series GSE113988. Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S. 6 samples. Type: Expression profiling by high throughput sequencing.
The immunogenic potential of bacterial flagella for Salmonella-mediated tumor therapy
GEO Series GSE116623. Salmonella enterica subsp. enterica serovar Typhimurium. 6 samples. Type: Expression profiling by high throughput sequencing.
The effect of ionomycin-induced oocyte activation on multiple morphological abnormalities of the sperm flagella
GEO Series GSE216618. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Global transcriptional analysis of Campylobacter jejuni NCTC 11168 after inactivation of flagella biosynthesis regulator FlhF
GEO Series GSE87852. Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819. 6 samples. Type: Expression profiling by array.
A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella
GEO Series GSE65247. Mus musculus. 12 samples. Type: Expression profiling by array.
The interplay between a protein and an mRNA antagonist controls CsrA-mediated regulation of flagella genes in Campylobacter jejuni
GEO Series GSE58419. Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; Campylobacter jejuni subsp. jejuni 81-176. 4 samples. Type: Expression profiling by high throughput sequencing.
Sigma 28-dependent small RNAs regulate timing of flagella biosynthesis
GEO Series GSE174487. Escherichia coli K-12. 16 samples. Type: Expression profiling by high throughput sequencing.
Host Acid Signal Controls Salmonella Flagella Biogenesis through CadC-YdiV Axis
GEO Series GSE210570. Salmonella enterica subsp. enterica serovar Typhimurium. 6 samples. Type: Expression profiling by high throughput sequencing.
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