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Dataset results
16 results for “fluidigm”
Data from: Don't put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array
With the increasing democratization of high-throughout sequencing (HTS) technologies, along with the concomitant increase in sequence yield per dollar, many researchers are exploring HTS for microbial community ecology. Many elements of experimental design can drastically affect the final observed community structure, notably the choice of primers for amplification prior to sequencing. Some targeted microbes can fail to amplify due to primer-targeted sequence divergence and be omitted from obtained sequences, leading to differences among primer pairs in the sequenced organisms even when targeting the same community. This potential source of taxonomic bias in HTS makes it prudent to investigate how primer choice will affect the sequenced community prior to investing in a costly community-wide sequencing effort. Here we use Fluidigm's micro-fluidic Access Arrays (IFC) followed by Illumina® MiSeq Nano sequencing on a culture-derived local mock community to demonstrate how this approach allows for a low-cost combinatorial investigation of primer pairs and experimental samples (up to 48 primer pairs and 48 samples) to determine the most effective primers that maximize obtained communities whilst minimizing taxonomic biases.
Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community analyses using the Fluidigm Access Array
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Single cell RNA seq of adult mouse mammary gland using Fluidigm C1 platform.
GEO Series GSE155627. Mus musculus. 103 samples. Type: Expression profiling by high throughput sequencing.
Fluidigm single-cell RNA-seq analysis for gene signatures associated with T-cell persistence
GEO Series GSE136610. Homo sapiens. 237 samples. Type: Expression profiling by high throughput sequencing.
Fluidigm real-time PCR analysis of mouse tissue
GEO Series GSE141960. Mus musculus. 41 samples. Type: Expression profiling by RT-PCR.
Preparing for the first breath at single cell level [single cell Fluidigm C1]
GEO Series GSE122330. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
Measuring anti-islet autoimmunity in mouse and human by profiling peripheral blood antigen specific CD4 T cells [Fluidigm]
GEO Series GSE235123. Homo sapiens. 48 samples. Type: Expression profiling by RT-PCR.
Single cell transcriptomic profiling of pluripotent stem cell-derived SCGB3A2+ airway epithelium reveals fate plasticity* - Fluidigm C1: bronchospheres day 27*
GEO Series GSE103919. Homo sapiens. 76 samples. Type: Expression profiling by high throughput sequencing.
Fluidigm Biomark 96.96 nanofluidic arrays for qRT-PCR of 93 miRNAs: salivary extracellular vesicles
GEO Series GSE78763. Homo sapiens. 192 samples. Type: Expression profiling by RT-PCR.
Mouse models of neutropenia reveal progenitor-stage-specific defects [Fluidigm]
GEO Series GSE142347. Homo sapiens. 380 samples. Type: Expression profiling by high throughput sequencing.
Single-nucleus RNA-seq identifies divergent populations of FSHD2 myotube nuclei [Fluidigm]
GEO Series GSE143492. Homo sapiens. 317 samples. Type: Expression profiling by high throughput sequencing.
Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells
GEO Series GSE77980. Mus musculus. 622 samples. Type: Expression profiling by high throughput sequencing.
Fluidigm C1 + Illumina HiSeq quantitative whole transcriptome analysis of unsorted population of E16.5 lung cells
GEO Series GSE69761. Mus musculus. 148 samples. Type: Expression profiling by high throughput sequencing.
ThPOK is a critical and multifaceted regulator of myeloid lineage development [Fluidigm]
GEO Series GSE163843. Mus musculus. 96 samples. Type: Expression profiling by high throughput sequencing.
A cost effective single cell RNA sequencing approach for the Fluidigm C1
GEO Series GSE79136. Homo sapiens. 361 samples. Type: Expression profiling by high throughput sequencing.
A unified workflow to define multipotent progenitor hierarchies [Fluidigm]
GEO Series GSE266522. Mus musculus. 337 samples. Type: Other.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.