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ShareScore release 0.9.0
Dataset results
13 results for “fluorescence assay”
Data of quantitative, ratiometric dual fluorescence reporter assay at the single-cell level
<p>The data set shows the evaluation of a new dual fluorescence reporter assay using three test genes, vimentin, lamin A/C and Arp3. This fluorescence protein-based reported assay successfully predicts siRNA efficacy with high fidelity. Data include images of Hela cells transfected with Vim-T4 ad Vim-T5 knockdown contructs; images of dual fluorescence assay; data analysis for knockdown experiments, Western blotting analysis of vimentin-knockdown in Hela cells, knockdown of endogenous lamin A/C in Hela cells; quantification of lamin A/C knockdown in Hela cells; dual fluorescence assay of human vimentin genes using three plasmids; Western blotting of Arp3 knockdown in SCC9 cells.</p> <p>All files can be opened using LibreOffice or OpenOffice. Some of the ODS files have a minor problem when opening them in Excel in the Windows computers. Excel will give warning about some unreadable contents. By choosing the repair option, the file contents will be shown without changes compared to the original data. Detailed explanation is given in the “Explanation file”.</p>
Resazurin-based time-kill assay in Acinetobacter baumannii AB074. Fluorescence measurement data for excitation 544 nm, emission 590 nm, gain 1000.
<p>There were 21 groups: 3 groups of transferrin alone in 3 different concentrations (1/3, 1, and 3 times of its MIC); 4 groups of antibiotics alone (ciprofloxacin and meropenem in 1/3 and 1 MIC concentration each), 6 combo groups for ciprofloxacin and 6 combo groups for meropenem, 1 group of positive control with bacteria but without any drug and 1 group of negative control without bacteria, with RPMI only. 20 µL of 0.1% aqueous resazurin solution was added to each well and the plate was incubated at 37°C without shaking for 24 hours. After incubation fluorescence was measured (excitation 544 nm, emission 590 nm) at 0h, 1h, 2h, 4h, 6h, 8h, and 24 hrs using the FLUOstar Omega plate reader (BMG LABTECH GmbH, Germany). Plate layouts may vary.</p> <p>For more information contact author.</p>
USP5 Zf-UBD Purification & Fluorescence Polarization Displacement Assay Development
<p>USP5 zinc-finger ubiquitin binding domain (Zf-UBD) expression and purification from <em>E. coli, </em>and screening conditions for a fluorescence polarization (FP) displacement assay with a FITC-labelled ubiquitin peptide. </p>
Chromophore-driven optical path reduction as an indirect inner filter effect correction strategy in fluorescence microplate assays - experimental data
<p>Experimental data for the paper entitled <em>Chromophore-driven optical path reduction as an indirect inner filter effect mitigation strategy in fluorescence microplate assays</em> (<a title="Persistent link using digital object identifier" href="https://doi.org/10.1016/j.saa.2025.127049" target="_blank" rel="noreferrer noopener"><span><span>https://doi.org/10.1016/j.saa.2025.127049</span></span></a>). </p> <p>An Excel file with multiple worksheets is provided for the following:</p> <p>1. Fluorescence data:</p> <p>The worksheets contain fluorescence values measured in tetraplicates using two microplate readers (Tecan Spark M10, Tecan, Austria and SpectraMax iD3, Molecular Devices, USA), with baseline values recorded in octuplicates. The results were averaged and baseline-corrected. Detailed notes on the titration experiments are included in the manuscript. Data include both IFE-uncorrected fluorescence and fluorescence corrected (fully or partially) by the addition of an absorbing chromophore.</p> <p>2. Absorbance data:</p> <p>All measured absorbance values obtained using the Tecan Spark M10 microplate reader with UV-Vis transparent microplates are included. The absorbance values at specific wavelengths suitable for the Lakowicz IFE correction method or for determining the concentration of the compound are given separately.</p> <p>3. Fluorescein spectra:</p> <p>UV-Vis and fluorescence excitation/emission spectra for highly dilute fluorescein concentrations where the inner filter effect (IFE) is negligible are reported. These measurements were performed to obtain “true” excitation and emission spectra without the influence of IFE quenching effects.</p> <p>4. ZINFE/NINFE IFE corrections:</p> <p>The dataset contains the results of ZINFE/NINFE IFE corrections (<a href="https://doi.org/10.1021/acs.analchem.2c01031">https://doi.org/10.1021/acs.analchem.2c01031</a>) applied to the fluorescence data and processed using the web service available at <a href="https://ninfe.science">https://ninfe.science</a>.</p> <p>The notation of the fluorophores and microplates is the same as in the manuscript. For reasons of visual clarity, the numerical titration indices are not in subscript.</p> <p> </p>
USP5 Zf-UBD Fluorescence Polarization Displacement Assay Optimization
<p>Optimization of a fluorescence polarization (FP) assay for USP5 zinc finger ubiquitin binding domain (Zf-UBD) with an N-terminal FITC-labeled ubiquitin RLRGG peptide. </p>
USP5 Zf-UBD Fluorescence Polarization Displacement Assay #3
<p>Development of a fluorescence polarization (FP) assay for USP5 zinc finger ubiquitin binding domain (Zf-UBD) with different lengths of N-terminal FITC-ubiquitin peptides. </p>
Developing a Tryptophan Fluorescence Assay for Screening Ligands against USP5 Zf-UBD
<p>Development of a fluorescence based assay by measuring changes in UV tryptophan fluorescence of USP5 zinc finger ubiquitin binding domain (Zf-UBD) with addition of ligands</p>
Data from: Scalable, semi-automated fluorescence reduction neutralization assay for qualitative assessment of Ebola virus-neutralizing antibodies in human clinical samples
Antibody titers against a viral pathogen are typically measured using an antigen binding assay, such as an enzyme-linked immunosorbent assay (ELISA), which only measures the ability of antibodies to identify a viral antigen of interest. Neutralization assays measure the presence of virus-neutralizing antibodies in a sample. Traditional neutralization assays, such as the plaque reduction neutralization test (PRNT), are often difficult to use on a large scale due to being both labor and resource intensive. Here we describe an Ebola virus fluorescence reduction neutralization assay (FRNA), which tests for neutralizing antibodies, that requires only a small volume of sample in a 96-well format and is easy to automate. The readout of the FRNA is the percentage of Ebola virus-infected cells measured with an optical reader or overall chemiluminescence that can be generated by multiple reading platforms and the readout is compatible with lytic and non-lytic viruses. Using blinded human clinical samples (EVD survivors or contacts) obtained in Liberia during the 2013–2016 Ebola virus disease outbreak, we demonstrate that FRNA-measured antibody titers are highly correlated with those measured by the Filovirus Animal Non-clinical Group (FANG) ELISA - the current standard for anti-EBOV antibody measurement with the important distinction of providing information on the neutralizing capabilities of the antibodies.
Data from: Scalable, semi-automated fluorescence reduction neutralization assay for qualitative assessment of Ebola virus-neutralizing antibodies in human clinical samples
Open the record for dataset details and reuse information.
A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [MNase-seq]
GEO Series GSE249469. Saccharomyces cerevisiae. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae
GEO Series GSE249628. Saccharomyces cerevisiae; Saccharomyces cerevisiae S288C. 14 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae [RNA-seq]
GEO Series GSE249580. Saccharomyces cerevisiae S288C. 6 samples. Type: Expression profiling by high throughput sequencing.
Fluorescence wound edge assay
<p>RGB images (16-bit) of fixed and stained 3T3 cells in a wound edge assay. 6 conditions are available: unstimulated, serum, dmso, LPA, nocodazole, and cytochalasinD.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.