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20 results for “fluorescence spectroscopy”
Leaf spectroscopy and active fluorescence datasets for early drought and nitrogen stress diagnosis in tomato
<p>The dataset contains different plant physiological parameters collected during a 14-day stress and recovery experiment on tomato (<em>Solanum lycopersicum</em> L. cv Moneymaker) plants, undergoing a nitrogen deficiency, drought or control treatment. </p> <p>A full description of the experiment, together with the scientific results, is published by Pescador-Dionisio et al. (2024), and can be found through: <a href="https://doi.org/10.1111/nph.20253">https://doi.org/10.1111/nph.20253.</a></p> <p>The goal of the dataset collection was to obtain a non-invasive proximal sensing dataset at leaf level (reflectance, transmittance, upward and downward fluorescence), in parallel to gas exchange and active fluorescence measurements. The leaf spectroscopy dataset was further processed by a pigment spectral unmixing algorithm according to Van Wittenberghe et al. (2024), to calculate fluorescence quantum efficiency (<em><strong>FQE</strong></em>) and effective absorbance (<strong><em>A_eff</em></strong>) changes associated to the activation of regulated heat dissipation (<strong><em>A_eff_535_Xan</em></strong>). The latter absorption feature is linked to the xanthophyll ('<strong>Xan</strong>') absorption in the 500-600 nm range, which is modelled by the sum of three Gaussians. For a full description of this feature, see Van Wittenberghe et al. (2021).</p> <p>Gas exchange and active fluorescence measurements were carried out with a LI-6400 portable photosysthesis system (LI-COR Biosciences, Lincoln, USA) equipped with a 6400-40 leaf chamber fluorometer. Steady-state measurements were done at 300 and 1000 μmol m−2 s−1 ('<strong><em>PAR300</em></strong>' and '<em><strong>PAR1000</strong></em>'), i.e. growing light conditions and light saturating conditions. Light response curves were taken on different days. Common fluorescence parameters (e.g., <em><strong>Fv/Fm, Fo, Fm, NPQ, YNO, YNPQ</strong></em>) are provided together with 'sustained' and reversible' NPQ parameters calculated according Porcar-Castell (2011).</p> <p>Leaf spectroscopy and active steady-state fluorescence measurements were performed on the same measuring days ('<em><strong>d0</strong></em>', '<em><strong>d2</strong></em>', '<em><strong>d4</strong></em>', '<em><strong>d7</strong></em>', '<em><strong>d14</strong></em>') and on the same leaf, both at 300 and 1000 μmol m−2 s−1 ('<em><strong>PAR300</strong></em>' and '<em><strong>PAR1000</strong></em>'), taking into account an adaptation time. We used a LED light source and several filters, placed in front of a FluoWat leaf clip, which was connected to two high-performance VIS-NIR spectroradiometers (QEPRO, Ocean Insight Inc., Orlando, Florida, USA). The spectroscopy measurements are presented in the Matlab structures for each measuring day, e.g. "<strong><em>2023_d0_Leaf_Spec_Tomato_Stress.mat</em></strong>".</p> <p>The outputs of the pigment spectral fitting code are presented by Matlab structures, e.g. "<strong><em>2023_d0_Leaf_Fitting_Tomato_Stress.mat</em></strong>", which contains the effective absorbance fitting (<strong><em>A_eff</em></strong>) of each pigment (<strong>Chl a, Chl b, Carotene-b, Anthocyanins, and Xanthophylls</strong>) for the wavelength range [500-780] nm, the absorbed photosynthetically active radiation by Chlorophyll a ('<em><strong>APAR_Chla</strong></em>') for the wavelength range [400-800] nm, and the fluorescence quantum efficiency, calculated as the ratio of the emitted fluorescence photons and the flux of photons absorbed by Chlorophyll a. </p> <p>Additional metadata from HPLC photosynthetic pigment analyses, xanthophyll-related enzyme expression, biomass and total content of elemental nitrogen are provided.</p> <p>Please follow the README files for more detailed information.</p> <p> </p>
Allocation of rhodamine-loaded nanocapsules from blood circulatory system to adjacent tissues assessed in vivo by fluorescence spectroscopy
<p>Modern fluorescent modalities play an important role in the functional diagnostic of various physiological processes in living tissues. Utilizing the fluorescence spectroscopy approach we observe the circulation of fluorescent-labelled nanocapsules with rhodamine tetramethylrhodamine in a microcirculatory blood system. The measurements were conducted transcutaneously on the surface of healthy Wistar rat thighs in vivo. The administration of the preparation capsule suspension with a rhodamine concentration of 5 mg kg−1 of the animal weight resulted in a two-fold increase of fluorescence intensity relative to the baseline level. The dissemination of nanocapsules in the adjacent tissues via the circulatory system was observed and assessed quantitatively. The approach can be used for the transdermal assessment of rhodamine-loaded capsules in vivo.</p>
Analysis of heritage stones and model wall paintings by pulsed laser excitation of Raman, laser-induced fluorescence and laser-induced breakdown spectroscopy signals with a hybrid system
<p>Analysis of heritage stone samples, alabaster, gypsum, limestone and marble, and model wall paintings was carried out with a laboratory, hybrid system based on the pulsed laser excitation of Raman, laser-induced fluorescence and laser-induced breakdown spectroscopy signals. The system is based on a nanosecond Q-switched Nd:YAG laser operating at its second (532 nm), third (355 nm) and fourth (266 nm) harmonics and a spectrograph coupled to a time-gated intensified charge coupled device for spectral analysis allowing detection with temporal resolution. For the stone samples, Raman spectra display the characteristic vibration modes of SO<sub>4</sub><sup>2-</sup> of calcium sulfate, in alabaster and gypsum, and of free CO<sub>3</sub><sup>2- </sup>of calcium carbonate, in limestone and marble. Simultaneously acquired laser-induced fluorescence spectra reveal characteristic bands that help to distinguish between heritage stone types. The elemental composition of stone samples is obtained by laser-induced breakdown spectroscopy upon excitation at 355 nm. Spectra of all stone samples reveal their elemental composition that includes Ca, Na, Mn and Sr and the presence of molecular species, such as CN, C<sub>2</sub> and CaO. Additional emission lines, ascribed to Mg, Si, Al and K, appear with different intensities according to the nature of the stone material. Model wall paintings, based on a red pigment, prepared as fresco or mixed with two different binders, were also studied. The complementary information provided by the three spectroscopic modes allows the identification of the pigment as red vermillion and of the different preparations based on the pigment alone or in mixtures with linseed oil and egg yolk binders.</p>
Fluorescence correlation spectroscopy TCSPC data with and without peak artifacts - PEX5 applied experiment
<p>This is a dataset of Fluorescence Correlation Spectroscopy (FCS) Time-Correlated Single Photon Counting (TCSPC) data with and without peak artifacts. The provenance of the data is recorded in <a href="https://github.com/aseltmann/fluotracify/blob/data/data/exp-220120-correlate-ptu/LabBook-exp-220120-correlate-ptu.org">this file</a> (see a rendered version <a href="https://aseltmann.github.io/fluotracify/data/LabBook-all.html#sec-2-5">here</a>). This parent project (<a href="https://github.com/aseltmann/fluotracify">https://github.com/aseltmann/fluotracify</a>) also contains examples of how to use these models and related Python code.</p> <p>The following connected paper is currently under review and should be cited together with these model versions: <em>Seltmann, A.; Carravilla, P.; Reglinski, K.; Eggeling, E.; Waithe, D. Neural Network Informed Photon Filtering Reduces Artifacts in Fluorescence Correlation Spectroscopy Data. 2023 (currently under review)</em></p> <p><strong>Note on the file formats and notation:</strong></p> <p>"Primary data" refers to the <code>.ptu</code> files, so the actual TCSPC data. "Secondary data" refers to the <code>.pqres</code> files, which are derived files by the proprietary PicoQuant software.</p> <p><strong>Note on sample preparation (from the Supplementary Note of the paper above):</strong></p> <p>The peak artifacts measurements were produced by 20 nM Trypanosoma brucei-PEX5 N-term fused to eGFP in solution, and the corresponding control measurements by 5 nM Homo sapiens-PEX5 N-term fused to eGFP in solution. The detailed sample preparation is described elsewhere. We prepared the samples on #1.5 coverslips mounted on Attofluor Cell Chambers (Thermo Fisher Scientific). We acquired the data on a MicroTime 200 microscope (PicoQuant) equipped with an Olympus UPlanSApo 60× 1.2NA water immersion objective lens and a HydraHarp 400 TCSPC module (PicoQuant). Excitation was achieved with a 488 nm pulsed laser (PicoQuant) with a power of 5 mW measured at the sample plane. We used a 20 nM solution of Alexa Fluor for calibrating the correction collar. One TCSPC measurement had a length of 20 s for PEX5 experiments.</p>
Fluorescence correlation spectroscopy TCSPC data with and without peak artifacts - AlexaFluor 488 applied experiment
<p>This is a dataset of Fluorescence Correlation Spectroscopy (FCS) Time-Correlated Single Photon Counting (TCSPC) data with and without peak artifacts. The provenance of the data is recorded in <a href="https://github.com/aseltmann/fluotracify/blob/data/data/exp-220120-correlate-ptu/LabBook-exp-220120-correlate-ptu.org">this file</a> (see a rendered version <a href="https://aseltmann.github.io/fluotracify/data/LabBook-all.html#sec-2-5">here</a>). This parent project (<a href="https://github.com/aseltmann/fluotracify">https://github.com/aseltmann/fluotracify</a>]) also contains examples of how to use these models and related Python code.</p> <p>The following connected paper is currently under review and should be cited together with these model versions: <em>Seltmann, A.; Carravilla, P.; Reglinski, K.; Eggeling, E.; Waithe, D. Neural Network Informed Photon Filtering Reduces Artifacts in Fluorescence Correlation Spectroscopy Data. 2023 (currently under review)</em></p> <p><strong>Note on the file formats and notation:</strong></p> <p>"Primary data" refers to the <code>.ptu</code> files, so the actual TCSPC data. "Secondary data" refers to the <code>.pqres</code> files, which are derived files by the proprietary PicoQuant software.</p> <p><strong>Note on sample preparation (from the Supplementary Note of the paper above):</strong></p> <p>The peak artifact measurements were produced by mixing 20 nM of the small dye Alexa Fluor 488 (Thermo Fisher Scientific) with 10 μM of slow, large (100 nm) unilamellar vesicles (LUVs) labelled with DiO in the membrane (0.1 % mol lipid:dye). The corresponding control measurements were 20 nM Alexa Fluor 488 in solution. We prepared the LUVs by the extrusion method: 1−palmitoyl−2−oleoyl−glycero−3−phosphocholine (POPC, Avanti Polar Lipids) was vacuum dried on a glass vial for 1 h and resuspended on phosphate buffered saline to form lipid vesicles. The vesicles were extruded 30 times through 100 nm pore size polycarbonate membranes using a Mini-Extruder (Avanti Polar Lipids). We prepared the samples on #1.5 coverslips mounted on Attofluor Cell Chambers (Thermo Fisher Scientific). We acquired the data on a MicroTime 200 microscope (PicoQuant) equipped with an Olympus UPlanSApo 60× 1.2NA water immersion objective lens and a HydraHarp 400 TCSPC module (PicoQuant). Excitation was achieved with a 488 nm pulsed laser (PicoQuant) with a power of 5 mW measured at the sample plane. We used a 20 nM solution of Alexa Fluor for calibrating the correction collar. One TCSPC measurement had a length of 10 s for AF488 experiments.</p>
Fluorescence correlation spectroscopy time-series data with and without peak artifacts - simulated data
<p>This is a dataset of FCS time-series with and without peak artifacts. It was created by 2D Monte Carlo simulations of diffusing particles. The provenance of the data is recorded in <a href="https://github.com/aseltmann/fluotracify/blob/data/data/exp-201231-clustsim/LabBook-exp-201231-clustsim.org">this file</a> (see a rendered version <a href="https://aseltmann.github.io/fluotracify/data/LabBook-all.html#sec-2-2">here</a>). This parent project (<a href="https://github.com/aseltmann/fluotracify">https://github.com/aseltmann/fluotracify</a>]) also contains examples of how to use this data and related Python code to load it.</p> <p>The following connected paper is currently under review and should be cited together with this dataset: Seltmann, A.; Carravilla, P.; Reglinski, K.; Eggeling, E.; Waithe, D. Neural Network Informed Photon Filtering Reduces Artifacts in Fluorescence Correlation Spectroscopy Data. 2023 (currently under review)</p> Data structure inside each .csv file <table><tbody> <tr> <td><header></td> <td> <p>10 to 12 lines, contains metadata</p> </td> <td> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td>...</td> <td>...</td> <td> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td><source_1></td> <td><target_1a></td> <td><target_1b></td> <td><source_2></td> <td><target_2a></td> <td><target_2b></td> <td>...</td> </tr> <tr> <td> <p>FCS time-series with artifact</p> </td> <td>Artifact time series</td> <td> <p>FCS time series without artifact</p> </td> <td> <p>FCS time series with artifact</p> </td> <td>Artifact time series</td> <td> <p>FCS time-series without artifact</p> </td> <td>...</td> </tr> <tr> <td>...</td> <td>...</td> <td>...</td> <td>...</td> <td>...</td> <td>...</td> <td>...</td> </tr> </tbody> </table> <p> </p>
Molecular factors determining brightness in fluorescence-encoded infrared vibrational spectroscopy
<p>Uploaded to this link are the datasets used for calculating the FEIR activities of the normal modes of ten coumarins studied in this work. We recommend going through the readme file (readme_file.txt), which should guide the reader through the data files.</p>
tttrlib: modular software for integrating fluorescence spectroscopy and imaging
<p>This repository contains scripts and datasets associated with the manuscript "tttrlib: modular software for integrating fluorescence spectroscopy and imaging". The software is designed to facilitate the analysis of fluorescence spectroscopy and imaging data to enable integration with molecular modeling.</p> <p>The provided scripts encompass various functionalities, including:</p> <ul> <li>Single-molecule FRET (smFRET) analysis for studying conformational dynamics, particularly focusing on human guanylate binding protein 1 (hGBP1).</li> <li>Comprehensive image spectroscopy workflows applicable to murine guanylate binding protein 2 (mGBP2), featuring intensity and time-resolved analyses.</li> <li>Tools for burst variance analysis (BVA), multiparameter fluorescence detection, and correlative analysis of fluorescence lifetime and intensity.</li> </ul> <p>Datasets for both hGBP1 and mGBP2 are included to exemplify the software's capabilities in real-world applications.</p>
Data for "Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics"
<p>We provide the raw data for the paper "Shrinking gate fluorescence correlation spectroscopy yields equilibrium constants and separates photophysics from structural dynamics". The files hold time tagged and time resolved single photon data from experiments on immobilized structures as well on free diffusion structures. The files have PicoQuant's .ptu files-format.</p> <p>Additionally we provide the python scripts used to analyze these data sets.</p>
Identification of Goat Milk Adulterated with Cow Milk Based on Total Synchronous Fluorescence Spectroscopy
<p>This data comes from the article "<span>Identification of Goat Milk Adulterated with Cow Milk Based on Total Synchronous Fluorescence Spectroscopy Combined with CNN</span>". Among them, "origin_data" is the original experimental data; "data" is the original experimental data extracted and merged into Excel data; "plot_data" is the contour map drawn for a single sample. For detailed experimental configuration, please refer to the article. If you need detailed data analysis, please contact 19851781820@163.com.</p>
Fluorescence spectroscopy data (both regular and solid phase fluorescence spectroscopy)
<p>Data attached to an Emergent Scientist type of article, produced for IDCS class.</p>
Analysis of heritage stones and model wall paintings by pulsed laser excitation of Raman, laser-induced fluorescence and laser-induced breakdown spectroscopy signals with a hybrid system
<p>Laser based analysis of artworks benefits from the development of hybrid instruments where a single laser source serves to excite fluorescence, Raman and laser induced breakdown spectroscopy (LIBS) signals. Laser induced fluorescence (LIF) and Raman spectra provide information at the molecular level, while LIBS serves for identifying the elemental composition of the substrate under consideration. Studies using several excitation wavelengths on different types of materials and substrates help to develop and establish these hybrid systems for the conservation of artworks.</p>
Fluorescence Spectroscopy Guided Surgery
ClinicalTrials.gov study NCT02473380. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Fluorescence Spectroscopy for Gut Permeability Assessment
ClinicalTrials.gov study NCT03434639. IPD Sharing: YES. Countries: 1. Publications: 1.
Data from: Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy
To understand complex regulatory processes in multicellular organisms, it is critical to be able to quantitatively analyze protein movement and protein-protein interactions in time and space. During Arabidopsis development, the intercellular movement of SHORTROOT (SHR) and subsequent interaction with its downstream target SCARECROW (SCR) control root patterning and cell fate specification. However, quantitative information about the spatio-temporal dynamics of SHR movement and SHR-SCR interaction is currently unavailable. Here, we quantify parameters including SHR mobility, oligomeric state, and association with SCR using a combination of Fluorescent Correlation Spectroscopy (FCS) techniques. We then incorporate these parameters into a mathematical model of SHR and SCR, which shows that SHR reaches a steady state in minutes, while SCR and the SHR-SCR complex reach a steady-state between 18 and 24 hours. Our model reveals the timing of SHR and SCR dynamics and allows us to understand how protein movement and protein-protein stoichiometry contribute to development.
Data from: Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy
Open the record for dataset details and reuse information.
Biomonitoring of Environmental Pollution: An Exploratory Investigation Using Mosses and X-Ray Fluorescence(XRF) Spectroscopy
<p>Raw data and voucher information used for a study</p>
Measurement of Digital Colposcopy for Fluorescence Spectroscopy of Cervical Intraepithelial Neoplasia
ClinicalTrials.gov study NCT00513123. IPD Sharing: Not stated. Countries: 3. Publications: 0.
Fluorescence and Reflectance Spectroscopy During Colposcopy in Detecting Cervical Intraepithelial Neoplasia and Dysplasia in Healthy Participants With a History of Normal Pap Smears
ClinicalTrials.gov study NCT00084903. IPD Sharing: Not stated. Countries: 2. Publications: 0.
Measurement of Digital Colposcopy for Fluorescence Spectroscopy of TNC Using a Second Device
ClinicalTrials.gov study NCT00503919. IPD Sharing: Not stated. Countries: 0. Publications: 0.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.