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2 results for “flux balance analysis”
Distributed flux balance analysis simulations of serial biomass fermentation by two organisms
<p><span>Intelligent biorefinery design that addresses both the composition of the biomass feedstock as well as fermentation microorganisms could benefit from dedicated tools for computational simulation and computer-assisted optimization. Here we present the BioLego Vn2.0 framework, based on Microsoft Azure Cloud, which supports large-scale simulations of biomass serial fermentation processes by two different organisms. BioLego enables the simultaneous analysis of multiple fermentation scenarios and the comparison of fermentation potential of multiple feedstock compositions. Thanks to the effective use of cloud computing it further allows resource intensive analysis and exploration of media and organism modifications. We use BioLego to obtain biological and validation results, including (1) exploratory search for the optimal utilization of corn biomasses – corn cobs, corn fiber and corn stover – in fermentation biorefineries; (2) analysis of the possible effects of changes in the composition of <i>K. alvarezi</i> biomass on the ethanol production yield in an anaerobic two-step process (<i>S. cerevisiae</i> followed by <i>E. coli</i>); (3) analysis of the impact, on the estimated ethanol production yield, of knocking out single organism reactions either in one or in both organisms in an anaerobic two-step fermentation process of <i>Ulva</i> sp. into ethanol (<i>S. cerevisiae</i> followed by <i>E. coli</i>); and (4) comparison of several experimentally measured ethanol fermentation rates with the predictions of BioLego.</span></p>
Distributed flux balance analysis simulations of serial biomass fermentation by two organisms
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