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Fig 1. A parasitoid wasp and 1 in Predatory behavior of long-legged flies (Diptera: Dolichopodidae) and their potential negative effects on the parasitoid biological control agent of the Asian citrus psyllid (Hemiptera: Liviidae)

Fig 1. A parasitoid wasp and 1 of 7 species of predaceous long-legged flies collected in this study. The photograph is insufficient for identification. Although predation events could not be duplicated in captivity, the parasitoid wasp ap- pears to be within a size range that the long-legged fly would attack (e.g., Barrentine 2011). Scale bar = 2 mm.

Fig. 1 in Evaluation of mass trapping and bait stations to control Anastrepha (Diptera: Tephritidae) fruit flies in mango orchards of Chiapas, Mexico

Fig. 1. Devices used as treatments during field evaluations: a) 600 mL polyethylene terephthalate (PET) bottle with three 1 × 1 cm holes in the upper third, baited with 50 mL of hydrolyzed protein; b) 600 mL PET bottle with two 3 × 3 cm windows in the upper middle, baited with 150 mL of GF-120 Naturalyte (80 ppm); c) MS® is a commercial bait station consisting of a 2-piece, bottle-shaped device, with a transparent upper piece with three 3 × 3 cm windows in the middle part and a yellow bottom, baited with Atrayente® (mix of 30% hydrolyzed protein, 10% propylene glycol, 5% malathion, and adjuvants; d) MS2® is the same MS® device as in 'c' but with the upper piece containing three 1-cm-diameter holes in the middle part, baited with 250 mL of Cera Trap®; e) wax matrix bait station is a waxed green box with slots baited with BioLure® synthetic attractant (ammonium acetate and putrescine); f) INIFAP trap is a 2 L, 14-cm-diameter, and 14 cm high cylindrical container, with three 1-cm-diameter holes at mid-height, baited with 250 mL of Cera Trap®.

Fig. 4 in Evaluation of mass trapping and bait stations to control Anastrepha (Diptera: Tephritidae) fruit flies in mango orchards of Chiapas, Mexico

Fig. 4. Recapture of sterile Anastrepha ludens and Anastrepha obliqua in Multilure® traps in different treatments. For each species, trap capture percentages topped by the same letter are not significantly different (α = 0.05).

Fig. 2 in Evaluation of mass trapping and bait stations to control Anastrepha (Diptera: Tephritidae) fruit flies in mango orchards of Chiapas, Mexico

Fig. 2. Recapture percentages of sterile Anastrepha ludens (top) and sterile Anastrepha obliqua (bottom) in Multilure® traps located in plots with PET bottle mass trapping devices, ground-sprayed with GF-120 Naturalyte, or untreated (control) in a mango orchard. For each season, trap capture percentages topped by the same letter are not significantly different (α = 0.05).

Fig. 5 in Evaluation of mass trapping and bait stations to control Anastrepha (Diptera: Tephritidae) fruit flies in mango orchards of Chiapas, Mexico

Fig. 5. Recapture percentages of sterile and wild Anastrepha ludens and Anastrepha obliqua in 2 types of mass trapping devices. For each species and strain, trap capture percentages topped by the same letter are not significantly different (α = 0.05).

Fig. 3 in Evaluation of mass trapping and bait stations to control Anastrepha (Diptera: Tephritidae) fruit flies in mango orchards of Chiapas, Mexico

Fig. 3. Recapture percentages of sterile Anastrepha ludens and Anastrepha obliqua flies in Multilure® traps in plots with different bait station devices. For each species, trap capture percentages topped by the same letter are not significantly different (α = 0.05).

Fig. 9 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 9. Number and sex ratio of fruit fly collected from funnel traps in bitter gourd plot treated with Thai bait.

Fig. 8 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 8. Number and sex ratio of fruit fly collected from funnel traps in bitter gourd plot treated with Pinnacle bait.

Fig. 5 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 5. Species and number of fruit fly collected from funnel traps in angled luffa plots treated with Pinnacle bait.

Fig. 4 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 4. Percentage of infested fruits of bitter gourd in plots treated with Pinnacle, Thai baits and untreated. (Only Bactrocera cucurbitae and B. tau were found.)

Fig. 1 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 1. Funnel trap for dead fruit flies collecting in plots treated with baits (1=waxed cardboard funnel; 2=plastic basket; 3=plastic glass, 4=formalin (5%), 5=string and 6=post).

Fig. 6 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 6. Species and number of fruit fly collected from funnel traps in bitter guard plots treated with Pinnacle and the Thai baits (Both sexes were collected).

Fig. 7 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 7. Number and sex ratio of fruit fly collected from funnel traps in angled luffa plot treated with Pinnacle bait.

Fig. 2 in Efficacy Of Protein Bait Sprays In Controlling Fruit Flies (Diptera: Tephritidae) Infesting Angled Luffa And Bitter Gourd In Thailand

Fig. 2. Number of fruit flies collected from fallen flowers of angled luffa in plots treated with Pinnacle bait and untreated. (Only Bactrocera diversa was found.)

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Targeted DNA-seq analysis was performed on sorted population of CD45+/CD34+ HSPCs from control or FLI-1 modified mRNA treated mPB after co-culture with vascular niche cells

Human mPB HSPCs were harvested isolated and transduced with either control or FLI-1 modified mRNA. HSPCs were introduced into co-culture with vascular niche ECs. Cultures were harvested and CD45+/CD34+ HSPCs were resoerted and processed for trageted DNA-seq analysis. Contains raw FASTQ sequencing files, unfiltered VCFs, and curated results in an excel.

opencc-zeroMay 2022View details →

Genomic insights into evolution and control of Wohlfahrtia magnifica, a widely distributed myiasis-causing fly of warm-blooded vertebrates

Wohlfahrtia magnifica is a pest fly species, invading livestock in many European, African and Asian countries, and causing heavy agro-economic losses. In the life cycle of this obligatory parasite, adult flies infect the host by depositing the first-stage larvae into body cavities or open wounds. The feeding larvae cause severe (skin) tissue damage and potentially fatal infections if untreated. Despite serious health detriments and agro-economic concerns, genomic resources for understanding the biology of W. magnifica have so far been lacking. Here, we present a complete genome assembly from a single adult female W. magnifica using a Low-DNA Input workflow for PacBio HiFi library preparation. The de novo assembled genome is 753.99 Mb in length, with a scaffold N50 of 5.00 Mb, consisting of 16,718 predicted protein-encoding genes. Comparative genomic analysis revealed that W. magnifica has the closest phylogenetic relationship to Sarcophaga bullata followed by Lucilia cuprina. Evolutionary analysis of gene families showed expansions of 173 gene families in W. magnifica that were enriched for gene ontology (GO) categories related to immunity, insecticide-resistance mechanisms, heat stress response and cuticle development. In addition, 45 positively selected genes displaying various functions were identified. This new genomic resource contributes to the evolutionary and comparative analysis of dipterous flies and an in-depth understanding of many aspects of W. magnifica biology. Furthermore, it will facilitate the development of novel tools for controlling W. magnifica infection in livestock.

opencc-zeroAug 2023View details →

Data from: A novel protein-based fruit fly trap in melon flies Bactrocera cucurbitae for effective pest control management

Agriculture remains a major source of subsistence for local communities in India. However, agricultural yield can be strongly affected by agricultural pest outbreaks. This can result in economic losses for small-scale farmers who already experience socioeconomic challenges, such as lack of appropriate infrastructure and subsidies. Sophisticated pest management techniques (e.g., sterile insect technique) are less accessible to small farmers in developing countries and therefore, alternative cost-effective approaches for pest management are needed. Here, we report our findings of a three-year-long field trial (2018 to 2020) in India which was designed to test for the potential effectiveness of a novel, slow-release formulation protein-based trap, compared to standard Cuelure traps against melon flies Bactrocera cucurbitae (Diptera: Tephritidae). Protein-bait traps can attract flies from both sexes (as opposed to males-only, chemical traps), bearing the potential to have greater long-term impact on pest populations by decreasing future reproductive potential of trapped individuals. We found that Cuelure had overall higher trapping performance, while protein-bait traps, despite trapping at lower efficiency, were equally effective for males and females. Simulations with our field data revealed that protein-bait traps can have an 'inclusive' advantage by trapping females and thereby preventing future individuals. Overall, our study highlights the potential benefits of using this alternative trapping technique to supplement pest management in developing countries.

zenodo36/100

Safety, effectiveness, and skin immune response in a controlled human infection model of sand fly transmitted cutaneous leishmaniasis.

Safety, effectiveness, and skin immune response in a controlled human infection model of sand fly transmitted cutaneous leishmaniasis.Vivak Parkash1, Helen Ashwin1, Shoumit Dey1, Jovana Sadlova2, Barbora Vojtkova2, Katrien Van Bocxlaer1,3, Rebecca Wiggins1, David Thompson4 Nidhi Sharma Dey1, Charles L. Jaffe5, Eli Schwartz6, Petr Volf2, Charles J. N. Lacey1, Alison M. Layton1,3 * and Paul M. Kaye1,3 *. 1 York Biomedical Research Institute, Hull York Medical School, University of York, York, UK2 Department of Parasitology, Faculty of Science, Charles University, Viničná 7, Prague, Czech Republic3 Skin Research Centre, Hull York Medical School, York, UK4 York and Scarborough Teaching Hospitals NHS Foundation Trust, York, UK  5 Department of Microbiology and Molecular Genetics, Kuvin Center for the Study of Infectious and Tropical Diseases, IMRIC, The Hebrew University – Hadassah Medical School, Jerusalem, Israel6 The Center for Geographic Medicine and Tropical Diseases, Chaim Sheba Medical Center, and The School of Medicine, Tel Aviv University, Israel. *Corresponding authors: paul.kaye@york.ac.uk and alison.layton@hyms.ac.uk

opencc-by-4.0Oct 2023View details →

publicDec 2025View details →

Data and code from: Controlled hyperthermia by flying-foxes in the wild: Understanding mammalian tolerance to hotter summer conditions

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